• 대한수의학회지
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• 제29권4호
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• pp.469-474
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• 1989

The changes of plasma prostaglandin $E_2$ level, natural killer cell activity and tumor cell growth were assayed after transplantation of EL-4 leukemia cells in C57BL/6 mice. The results were summarized as follows; 1. Plasma prostaglandin $E_2$ level was increased in EL-4 bearing mice, but indomethacin treated mice group showed low level. 2. The tumor-derived prostaglandin $E_2$ inhibited the post-target binding cytolytic process of natural killer activity. 3. Indomethacin inhibited the growth of prostaglandin secreting EL-4 solid tumor.

• Journal of Korean Neurosurgical Society
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• 제29권10호
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• pp.1340-1344
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• 2000

Objective : A prospective biochemical assay of prostaglandin $E_2$ content in symptomatic lumbar disc materials was done in order to clarify the pathogenesis of lumbar radiculopathy. Patients and Methods : Forty-eight disc specimens were purified by a standard solid-phase extraction procedure and analyzed by an enzymelinked immunosorbent assay for prostaglandin $E_2$. Clinical and anatomic correlations were evaluated with analysis of variance and t-test. Results : Acute herniated lumbar disc diseases tended to be associated with a higher prostaglandin $E_2$ content than degenerative lumbar disc disease. Sequestered discs tended to be associated with a higher prostaglandin $E_2$ content than extruded discs, which also showed higher prostaglandin $E_2$ content than protruded ones. A sciatica and positive straight leg raising test appeared to be associated with a higher prostaglandin $E_2$ content than a negative test. Conclusion : This result suggests that the level of prostaglandin $E_2$ would be correlated with clinical symptom and sign in the inflammatory process of lumbar disc herniation.

• 대한화장품학회지
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• 제20권1호
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• pp.25-36
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• 1994

Gram 음성균의 세포벽 성분인 lipopolysaccharide는 각종 세포에서의 prostaglandin 합성을 증진 시키며 이는 cyclooxygenase-2의 선택적 발현에 기인한다는 사실이 이미 보고된 바 있다. 본 연구에서는 mouse peritoneal marophage를 대상으로 하여 LPS의 prostaglandin 합성 증진 작용에 대한 특성으로 검토함으로써, COX-2에 대한 선택적 저해제 검색에 이용될 수 있는지 그 가능성을 확인코자 하였다. LPS는 peritoneal macrophage에 처리시 약 8시간 정도의 lag time을 보인 후 prostaglandin 합성을 현저히 증진 시켰으며, 이는 주로 COX 활성의 증가에 기인하는 것으로 추정되었다. 또한 LPS의 작용은 항염증제인 dexamethasone에 의해서 강하게 억제 되었으며 metabolic labeling 결과 이는 COX-2의 생합성을 억제하는데 기인하는 것으로 확인되었다. 따라서 mouse peritoneal macrophage에서의 LPS에 의한 prostaglandin 합성 증진 작용은 rat alveolar macrophage와 정성적으로 동일함을 확인할 수 있었으며, 본 실험 조건은 COX-2에 대한 선택적 저해제 검색에 응용될 수 있음을 확인 하였다. 본 실험 조건하에서 비스테로이드성 항염제인 ketoprofen의 작용을 검토한 결과 ketoprofen은 COX-1에 비교적 선택적인 저해 작용을 보이는 것으로 추정 되었다.

• Archives of Pharmacal Research
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• 제21권3호
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• pp.361-362
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• 1998

• 대한수의학회지
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• 제27권2호
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• pp.185-189
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• 1987

The number of splenic lymphocyte, serum prostaglandin $E_2$ level and natural killer cell activity were assayed after single whole body irradiation of a sublethal dose of $^{60}Co-{\gamma}$ ray to C57BL/6J mice. With a view to knowing the relationships between radiation induced prostaglandin $E_2$ level and the normal natural killer cell activity after natural killer cell-target cell conjugation, The change of normal natural killer cell activity were measured by administration of prostaglandin $E_2$ containing serum from irradiated mice. The results were summarized as follows; 1. The total number of splenic lymphocyte was significantly decreased by irradiation and the number was not affected by indometacin, prostaglandin synthesis inhibitor, treatment. 2. Serum prostaglandin $E_2$ level was increased in irradiated mice, but indometacin treated mice group showed low level of prostaglandin $E_2$. 3. In the case of irradiated mice, natural killer cell activity was not shown any difference between irradiated group and indometacin combined group. But when natural killer cell-target cell conjugations were exposed to the serum of each group during cytotoxic activity assay, whereas the normal natural killer cell activity was significantly decreased by treatment of serum from irradiated mice, the activity was not changed by treatment of indometacin pretreated mice serum. This result indicated that the prostaglandin $E_2$ induced by the radiation inhibited the post-target binding cytolytic process of natural killer activity.

• 대한약리학회지
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• 제23권1호
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• pp.51-56
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• 1987

본 실험실에서는 회양목(Buxus microphylla var. koreana Nakai)으로 부터 steroid성 alkaloid인 cyclobuxine D를 분리하였고, 그의 여러 약리작용을 검색하고 있다. 본 연구에서는 cyclobuxine D의 guinea pig lung homogenate에서 prostaglandins의 합성에 미치는 영향과 carrageenin으로 유도한 염증에서 prostaglandin의 합성과 백혈구 유주에 대한 영향을 관찰하였다. Cyclobuxine D (up to 100ug/ml)는 guinea pig lung homogenate에 의한 prostaglandins 합성에 대해서는 현저한 영향이 없었으나, 20mg/kg에서 carrageenin으로 유도된 염증에서 prostaglandin의 합성과 백혈구 유주에 대해 현저한 억제작용을 나타낸다. Aspirin은 vivo와 vitro에서 prostaglandin의 합성을 억제하나, 염증 삼출물에서 백혈구 유주에 대해서는 영향이 거의 없다. Dexamethasone은 vitro에서 외인성 arachidcnic acid를 기질로 가했을때는 prostaglandin 합성에 대해 영향이 없었고 carrageenin으로 유도된 염증에서 prostaglandin의 합성과 백혈구 유주를 억제하였다. 이상의 결과로 cyclobuxine D의 항염증작용은 phospholipase $A_2$ activity를 저해하여 항염증 작용을 나타내는 것으로 사료되는 corticosteroid와 유사한 것으로 추정된다.

• 대한수의학회지
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• 제19권2호
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• pp.115-119
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• 1979

Prostaglandin이 배란에 미치는 영향을 관찰하기 위하여 정상군과 양쪽 자궁을 제거한 가토에 indomethacin(prostaglandin 합성억제제)을 투여한 후 HCG데 의해 유발된 배란부위를 조직학적으로 관찰하였던 바 아래와 같은 결과를 얻었다. 1. indomethacin (10 mg/kg bw) 투여로 배란은 억제되었으며 투여량에 비례하여 배란은 억제되었다(p<0.01). 2. 자궁적출 단독으로는 배란에 영향을 주지 못하였다(p>0.05). 3. indomethacin은 난포파열을 억제함으로써 배란을 억제한다. 4. 토끼에서는 자궁이 아닌 장기에서 유래된 prostaglandin이 난포파열과정에 작용함으로써 배란을 시키는 작용이 있는 것으로 추정된다.

• The Korean Journal of Pain
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• 제19권2호
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• pp.168-174
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• 2006

Background: Several biochemical mediators, such as substance P, calcitonin gene-related peptide (CGRP) and prostaglandin $E_2$, have been demonstrated to be involved in herniated or degenerated disc-induced radiculopathy. The authors tested the hypothesis that these mediators would existed in the epidural space of humans. Methods: Thirty nine patients were divided into two groups; 27 patients, who were diagnosed with spinal stenosis (stenosis group), and 12 scheduled for epidural anesthesia, without a history of back pain (control group). Under fluoroscopic guidance, an epidural catheter was introduced through the caudal space and placed into the anterior and posterior spaces, up to and around the epidural adhesive area, in the stenosis group. In the control group, the catheter was placed into the posterior epidural space through the L3⁣-4 or L4⁣-5 intervertebral space. Epidural irrigation was performed with 10 ml of saline, via an epidural catheter. Aspirated lavage fluid was collected, and the concentrations of biochemical mediators (substance P, CGRP and prostaglandin $E_2$) measured using an enzyme immunoassay kit. Results: Substance P, CGRP and prostaglandin $E_2$ were detected in all the epidural lavage fluids from both groups. The concentrations of substance P and prostaglandin $E_2$ in the stenosis group were higher than those of the control (P < 0.05). However, there was no difference in the CGRP levels between the two groups. In the stenosis group, the concentrations of these three mediators in the anterior epidural space were no different to those in the posterior space. Conclusions: These results suggest that biochemical mediators, such as substance P and prostaglandin $E_2$, in the epidural space might be partly involved in pain mechanism associated with spinal stenosis.

• 국제물리치료학회지
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• 제9권3호
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• pp.1558-1563
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• 2018

This study, based on physical therapy interventions for menstruation disorders, observed the effect of Pilates stabilization exercises and Kinesio taping on dysmenorrhea and prostaglandin $F2{\alpha}$ levels. Female college students (n=37) who had >70 points on the mood disorder questionnaire (MDQ) and >4 points on the visual analogue scale (VAS) were divided into three different groups, the Pilates group (PG, n= 13), Pilates and Taping group (PTG, n=12), Taping group (TG, n=12). Pilates and taping were performed 3 times a week for 6 weeks. Pre- and post-menstruation difficulties were measured through the MDQ. Changes in blood prostaglandin $F2{\alpha}$ levels were also measured. Changes in pre- and post-menstrual pain and prostaglandin $F2{\alpha}$ levels were significantly reduced in the PG, PTG, and TG. Changes in pre- and post-menstrual pain and prostaglandin $F2{\alpha}$ levels resulted in significant differences in the PTG and TG. Dysmenorrhea and prostaglandin $F2{\alpha}$ levels were significantly decreased in the TG than in the PTG. These findings suggest that Pilates stabilization exercises and Kinesio taping are effective in decreasing menstrual difficulties and pain in female college students.

• Journal of Acupuncture Research
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• 제27권3호
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• pp.65-73
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• 2010

Objectives : Recently, Pharmacopuncture therapy has been used for the treatment of inflammatory diseases such as rheumatoid arthritis. Especially, we have been interested in chemical mediators concerned with inflammation such as prostaglandin, nitric oxide. The purpose of this study is investigated that the effect of Ampelopsis Radix Pharmacopuncture solution in RAW 264.7 macrophages, performed several experimental items : those are Prostaglandin $E_2$, Nitric Oxide and Cyclooxygenase-2. Methods : The cytotoxicity of Ampelopsis Radix Pharmacopuncture solution in RAW 264.7 macrophages were measured by MTT assay. In order to observe cyclooxygenase-2 mRNA expression in lipopolysaccharide and interferon-gamma stimulated RAW 264.7 macrophages, RT-PCR was used. Prostaglandin $E_2$ production and Nitric Oxide production was measured by nitric oxide detection kit and Prostaglandin $E_2$ assay kit. Results : 1. The MTT assay demonstrated that cytotoxic effect of Ampelopsis Radix Pharmacopuncture solution in RAW 264.7 macrophages was not appeared. 2. Ampelopsis Radix Pharmacopuncture solution inhibited nitric oxide production in lipopolysaccharide and interferon-gamma stimulated RAW 264.7 macrophages. 3. Ampelopsis Radix Pharmacopuncture solution inhibited cyclooxygenase-2 mRNA expression in lipopolysaccharide and interferon-gamma stimulated RAW 264.7 macrophages. 4. Ampelopsis Radix Pharmacopuncture solution inhibited Prostaglandin $E_2$ production in lipopolysaccharide and interferon-gamma stimulated RAW 264.7 macrophages. Conclusions : On the basis of these results, It was shown that Ampelopsis Radix Pharmacopuncture solution was able to inhibit the production of $PGE_2$ and NO, as well as COX-2 mRNA expression. Our results may provide new mechanism by which Ampelopsis Radix Pharmacopuncture solution accounts for its beneficial effect on accelerating wound healing and anti-inflammation.