• BMB Reports
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• v.37 no.3
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• pp.269-274
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• 2004

DNA polymerases without the 3' exonuclease function ($exo^-$ pol) have been widely used in sequencing and SNP genotyping. As a major player that expedited the coming of the postgenomic era, $exo^-$ polymerases worked remarkably well in the Human Genome Sequencing Project. However, it has become a challenge for this class of polymerases to efficiently screen the large number of SNPs that are found in the human genome. For more than three decades it has been recognized that polymerase fidelity varied according to the presence of proofreading activity that is mediated by its internal 3' exonuclease. Polymerases with proofreading function are famous for their high fidelity in DNA replication both in vivo and in vitro, but this well-known class of polymerases has been almost completely neglected in genetic analysis in the postgenomic era. We speculate that $exo^+$ polymerases may exhibit higher nucleotide identification ability when compared to $exo^-$ polymerases for an in vitro genetic analysis. With the application of $exo^+$ polymerases in SNP assays, a novel mechanism for the maintenance of DNA replication, the on/off switch, was discovered. Two new SNP assays have been developed to carry out genome-wide genotyping, taking advantage of the enzymatic properties of $exo^+$ polymerases. Furthermore, the on/off switch mechanism embodies a powerful nucleotide identification ability, which can be used to discriminate the bases that are upstream of the 3' terminus, and thus defines a new concept in de novo sequencing technology. Application of $exo^+$ polymerases to genetic analysis, and especially SNP assays, will greatly accelerate the pace to personalized medicine.

• Archives of design research
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• v.15 no.3
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• pp.93-102
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• 2002

The changing pattern of living and communication, combined with wireless technologies has given rise to more fluidity between spaces and relationships. Despite this, the way we use technology and the qualities of product and service are limited. For example, ON or OFF is like BLACK or WHITE, it presents a harsh distinction This severity in definition can make me uncomfortable when using products. The aim of this project is to improve the use of a product's functions and Qualities. As an example, the switch button has limited the possible interaction of a product. even each function is different. 1 intend this study is to imbue the switch with a greater freedom of interpretation. What will happen If I create a journey in between on and off\ulcorner For instance, time, pattern, state, rhythm and so on are analogue properties of a switch. The playful, spontaneous, and enjoyable behaviour of a switch will be able to bring other qualities of function and service. The switch will then be able to be an object with more human qualities, not simply an on and off button.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2014.11a
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• pp.78-80
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• 2014

Silicone structural glazing (SSG) is a method utilizing a silicone adhesive to attach glass, metal, or other panel material to the structure of a building. Windload and other impact loads on the facade are transferred from the glass or panel through the silicone structural sealant to the systems' framework. Silicone structural glazing systems are currently a very common method of glazing throughout the world. Locally, structural silicone glazing has become very common to achieve aesthetically pleasing and high utilization of small land for both residential and commercial building. Although structural silicone glazing has been utilized for approximately thirty years in Korea, the understanding of its technology was low and limited. Consequently, Korean projects experienced many quality issues during assembly and construction, even in very recently finished buildings. Adhesion loss and water infiltration occurred on more than one project, and the time and cost to repair these issues were substantial. In general, there are two kinds of structural silicones depending on fabrication methods. 1part structural silicone is for site glazing system and 2part structural silicone is for unitized factory glazing system. In this paper, 2part structural silicone which is very common for factory fabricating curtainwall systems was evaluated with regards to various mixing ratio. Since the structural performance of 2part sealant can be affected by mixing ratios, some extra ranges of recommended mixing ratio were evaluated to see any performance differences. Besides on cure profile, comparative evaluations for mechanical properties and adhesion develop on common building substrates were conducted.

• International conference on construction engineering and project management
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• 2011.02a
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• pp.213-220
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• 2011

In the current construction industry where various stakeholders take part, BIM Data exchange using standard format can provide a more efficient working environment for related staffs during the life-cycle of the building. Currently, the formats used to exchange the data from 3D-CAD application to structure energy analysis at the design stages are IFC, the international standard format provided by IAI, and gbXML, developed by Autodesk. However, because of insufficient data compatibility, the BIM data produced in the 3D-CAD application cannot be directly used in the energy analysis, thus there needs to be additional data entry. The reasons for this are as follows: First, an IFC file cannot contain all the data required for energy simulation. Second, architects sometimes write material names on the drawings that are not matching to those in the standard material library used in energy analysis tools. DOE-2.2 and Energy Plus are the most popular energy analysis engines. And both engines have their own material libraries. However, our investigation revealed that the two libraries are not compatible. First, the types and unit of properties were different. Second, material names used in the library and the codes of the materials were different. Furthermore, there is no material library in Korean language. Thus, by comparing the basic library of DOE-2, the most commonly used energy analysis engine worldwide, and EnergyPlus regarding construction materials; this study will analyze the material data required for energy analysis and propose a way to effectively enter these using semantic web's ontology. This study is meaningful as it enhances the objective credibility of the analysis result when analyzing the energy, and as a conceptual study on the usage of ontology in the construction industry.

• The Journal of the Korea Contents Association
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• v.14 no.2
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• pp.172-180
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• 2014

The purpose of this study is to examine how the Munjeonsa(SPC) has influenced the way terrestrial broadcasting networks produce drama. The study has three research objectives. First, this study investigates why the networks use SPC for drama production. Second, the study examines the features in the process of the setup and operation of SPC. Third, the study identifies the impacts of SPC on networks' drama production. This study analyzes which was produced by KBS SPC. The results of this study are as follows. First, networks introduce SPC to procure an enormous production costs and to manage the production system transparently and efficiently. Second, the feature of networks' SPC(KGCS SPC) is that it has a unique dual structure with main and project SPC. Third, SPC influences drama production system in various ways; revitalization of external investment, accounting transparency, growing of network's planning dramas, securing intellectual properties and revenue diversification.

• Tunnel and Underground Space
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• v.13 no.6
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• pp.455-464
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• 2003

Four assessment systems for planning and evaluation of TBM tunnelling are discussed, and their characteristics and input data are analyzed. Two of the systems are determined to be adequate for post-evaluation of TBM performance because the time, such as repair time, downtime, installation time and transport time, must be included for calculations. The others are adequate for pre-planning because the basic data of the systems consist of only the basic properties of rocks and rock masses, and the specification of TBM. In order to apply these assessment systems, a number of equations, graphs and charts are generally required, which seems to be very inconvenient and complicated. In this study, therefore, a user-friendly program operated on Windows system is developed, and each system can be selected by the corresponding input data. It will be possible fer tunnel engineers to select a system according to their objectives and available input data, and to apply the system to TBM tunnel project.

• Journal of the Institute of Electronics and Information Engineers
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• v.51 no.2
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• pp.148-155
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• 2014

In this paper a commute time embedding is implemented by organizing patches according to the graph-based metric, and its properties are investigated via changing the number of nodes on the graph.. It is shown that manifold embedding methods generate the intrinsic geometric structures when waveforms such as speech or music instrumental sound signals are embedded on the low dimensional Euclidean space. Basically manifold embedding algorithms only project the training samples on the graph into an embedding subspace but can not generalize the learning results to test samples. They are very effective for data clustering but are not appropriate for classification or recognition. In this paper a commute time guided transform is adopted to enhance the generalization ability and its performance is analyzed by applying it to the classification of 6 kinds of music instrumental sounds.

• Journal of the Korea Convergence Society
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• v.8 no.5
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• pp.137-143
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• 2017

Augmented reality makes people feel realistic and reduces the damage to cultural properties that can come into contact with the actual cultural heritage. Augmented reality has the advantage that it can show the past appearance of the historical building or relics, the environment, or related information in addition to the current real environment. Despite these values ​and advantages, As a result of researches and analyzes of the Augmented Reality contents of the National Museum, 'palace in my hand', and the Miruksa project In the field of domestic cultural heritage warriors, augmented reality technology has shown a very negative attitude such as simply showing cultural heritage content or using it as a guide. However, various conditions such as display and sensor hardware and technology needed to realize augmented reality more effectively are improving day by day. And If you have various facilities such as the idea of ​adding storytelling to the exhibits and WiFi building, In the near future, it will be equipped with a display system of cultural heritage contents that combines augmented reality with a more complete one.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.1
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• pp.242-249
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• 2010

As the scale of domestic building construction becomes larger, more complicated and more specialized, demands for quality improvement, cost reduction and construction period shortening increase. Construction materials management becomes a main factor to perform the project successfully. Therefore, various researches using RFID(Radio Frequency Identification) are being studied to manage the construction materials efficiently. This research aims to suggest the method of construction materials management using RFID in apartment housing. First, the technical properties of RFID are grasped. Second, problems are extracted by analyzing the case studies and related research using RFID in construction field. Third, construction materials are classified according to the construction process and process of materials management is analyzed. Lastly, method of construction materials management using RFID is suggested. The results of this research are expected to contribute the improvement of construction productivity through effective material management.

• Smart Structures and Systems
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• v.16 no.3
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• pp.383-399
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• 2015

Today, as railways increase their capacity and speeds, it is more important than ever to be completely aware of the state of vehicles fleet's condition to ensure the highest quality and safety standards, as well as being able to maintain the costs as low as possible. Operation of a modern, dynamic and efficient railway demands a real time, accurate and reliable evaluation of the infrastructure assets, including signal networks and diagnostic systems able to acquire functional parameters. In the conventional system, measurement data are reliably collected using coaxial wires for communication between sensors and the repository. As sensors grow in size, the cost of the monitoring system can grow. Recently, auto-powered wireless sensor has been considered as an alternative tool for economical and accurate realization of structural health monitoring system, being provided by the following essential features: on-board micro-processor, sensing capability, wireless communication, auto-powered battery, and low cost. In this work, an original harvester device is designed to supply wireless sensor system battery using train bogie energy. Piezoelectric materials have in here considered due to their established ability to directly convert applied strain energy into usable electric energy and their relatively simple modelling into an integrated system. The mechanical and electrical properties of the system are studied according to the project specifications. The numerical formulation is implemented with in-house code using commercial software tool and then experimentally validated through a proof of concept setup using an excitation signal by a real application scenario.