• Korean Journal of Oriental Medicine
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• v.4 no.1 s.4
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• pp.27-45
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• 1998

$\ulcorner$Euibangyoochui醫方類聚$\lrcorner$ (1445) is regarded as a treasure-house of the knowledge of traditional oriental medicine which contains over 50,000 prescriptions and enormerous amount of medical information. Despite the importance and information contained in this book, it has been rarely used since it was not convenient to use this book. In this study, therefore, the establishment of database on $\ulcorner$Euibangyoochui$\lrcorner$ was carried out. Before the database establishment of $\ulcorner$Euibangyoochui$\lrcorner$ , basic works such as correction, interpretation, proofreading and translation of original text should be done. The results obtained in this study are summaried as follows : 1) During the course of studying the original text of $\ulcorner$Euibangyoochui$\lrcorner$ , the editing process and transmission of medical books in early Chosun dynasty was figured out. 2) For better correction, interpretation, proofreading and translation of $\ulcorner$Euibangyoochui$\lrcorner$ , $\ulcorner$Euibangyoochui$\lrcorner$ microfilms which are the collection of Japanese Royal Library (宮內廳 圖書寮) were obtained in this study. Through this process, the errors in the republication were able to be corrected. 3) Analyzing the organization and compilatory method of $\ulcorner$Euibangyoochui$\lrcorner$ is one of the basic requirements of understanding the scale of the whole. book and establishing database as a result. So the analysis results were used for the basic structuring of database. 4) $\ulcorner$Euibangyoochui$\lrcorner$ CD- ROM was designed in a way that the images of microfilms, original text and Korean translation can be compared by 3-D device. In addition, the convenience and proficiency of imaging the information and prescriptions of the text is one of the remarkable features of this CD-ROM.

• Journal of the Daesoon Academy of Sciences
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• v.19
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• pp.21-45
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• 2005

The purpose of this study is to examine the establishment and development of Daesoon Thought Thesaurus. Specifically, this study examined the matters to be considered in the stage of Thesauri planning according to the Thesauri Construction process : presents the methods and standards of Thesauri Construction according to processes such as identification of the indexing policy, establishment of Thesauri system, collection of vocabulary, selection of preferred term, clustering of the terms, establishment of term relationships, overall adjustment, Thesauri test, proofreading by professional display, maintenance and updating. Since religion information is unique or totally different from the information in other areas, it is most important to construct the Thesauri suitable for system after carefully recognizing the concept of religion terms.

• Journal of the Korean Society of Food Culture
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• v.39 no.2
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• pp.83-95
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• 2024

『Hasimdangga Eumsikbeop』 is the second cookbook discovered in the Honam region. It is believed to have been copied by Hong Ju-song between the late 1800s and early 1900s, and excluding the missing parts, it contains a total of 14 items, including 7 liquor items and 7 general food items. Judging from the proofreading marks, it was not considered a complete creation. However, it was confirmed that the book was not influenced by existing ancient cookbooks from the Seoul-Gyeonggi, Chungcheong, and Yeongnam areas. Therefore, it is highly likely that the book was based on an undiscovered recipe book from an ancient family. It is valuable as a basis for comparative research on regional food culture in the traditional era in that the manufacturing methods, ingredient quantities, and description methods are different from existing cookbooks in many ways and contain unique terminologies and regional dialects.

• Journal of Microbiology and Biotechnology
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• v.8 no.5
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• pp.471-477
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• 1998

Taq DNA polymerase from Thermus aquaticus is very useful in the polymerase chain reaction. Taq DNA polymerase is classified in the pol I family, represented by E. coli DNA polymerase I. The three-dimensional structural alignment of 3'-5'exonuclease domains from the pol I family DNA polymerases explains why Taq DNA polymerase does not carry out proofreading in polymerase chain reactions. Three sequence motifs, Exo I, II, and III, must exist to carry out 3'-5'exonuclease activity for proof- reading by a 3'-5'exonuclease reaction, but these are abolished in Taq DNA polymerase. The key catalytic module in 3'-5'exonuclease is two metal ions chelated by four active-site carboxylic amino acids. Taq DNA polymerase was mutagenized to construct the catalytic module in the active site. The circular dichroism technique supported the formation of the catalytic module, and the radioactive assay showed that the 3'-5'exonuclease activity doubled in the mutant Taq DNA polymerase.

• Journal of Microbiology and Biotechnology
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• v.14 no.5
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• pp.1022-1030
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• 2004

The gene encoding Aquifex pyrophilus (Apy) DNA polymerase was cloned and sequenced. The Apy DNA polymerase gene consists of 1,725 bp coding for a protein with 574 amino acid residues. The deduced amino acid sequence of Apy DNA. polymerase showed a high sequence homology to Escherichia coli DNA polymerase I-like DNA polymerases. It was deduced by amino acid sequence alignment that Apy DNA polymerase, like the Klenow fragment, has only the two domains, the $3'{\rightarrow}5'$ exonuclease domain and the $5'{\rightarrow}3'$ polymerase domain, containing the characteristic motifs. The Apy DNA polymerase gene was expressed under the control of T7lac promoter on the expression vector pET-22b(+) in E. coli. The expressed enzyme was purified by heat treatment, and Cibacron blue 3GA and $UNO^{TM}$ Q column chromatographies. The optimum pH of the purified enzyme was 7.5, and the optimal concentrations of KCl and $Mg^{2+}$ were 20 mM and 3 mM, respectively. Apy DNA polymerase contained a double strand-dependent $3'{\rightarrow}5'$ proofreading exonuclease activity, but lacked any detectable $5'{\rightarrow}3'$ exonuclease activity, which is consistent with its amino acid sequence. The somewhat lower thermostability of Apy DNA polymerase than the growth temperature of A. pyrophilus was analyzed by the comparison of amino acid composition and pressure effect.

• BMB Reports
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• v.37 no.2
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• pp.167-176
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• 2004

Although efficient antiviral lamivudine is used for HBV-infected patients, a prolonged treatment with nucleoside analogs often results in lamivudine-resistant variants. In this study, we evaluated the fidelity of the lamivudine-resistant variants. The FLAG-tagged wild-type (FPolE) and Met550 variants (FPolE/M550A, M550V, and M550I) of HBV DNA polymerases were expressed in insect cells then purified. Like many other reverse transcriptases, no $3'{\rightarrow}5'$ exonuclease activity was detected in the HBV DNA polymerase. Since there is no proofreading activity, then the use of the site-specific nucleotide misincorporation method is beneficial. From the $f_{ins}$ value analysis, it is evident that M550I and M550V exhibit higher fidelity values than the wild-type HBV DNA polymerase, while M550A exhibits similar fidelity values. It is therefore suggested that lamivudine resistance comes from the stringency to dNTP binding and the discrimination of dCTP and lamivudine in M550V and M550I.

• The Journal of Korean Academy of Orthopedic Manual Physical Therapy
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• v.11 no.2
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• pp.19-25
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• 2005

Purpose : Facial palsy goes together not only physical difficulties but also social life's of relationship to other people. Therefore we was devised correction splint and rehabilitation set for facial palsy proofreading. Method : This article was used by questionnaire survey that intended for 140 patients who had got facial palsied such as universities hospitals and oriental hospitals over the country in Korea. The subject matter that faced consciousness of a patient as opposed the awkward rehabilitating tool that a general matter and patient. In the object that the rehabilitation tool which now patient used through a wraps a face in only as a treatment object. Results : 1. The most chief complaints among the facial palsy patients were eating activity (41%), relationship to other people (29%), communication (20% )(Fig. 3). 2. The most needs of the facial palsy patients was aids for early treat (53%), prevented face deformity (16%) etc, (Fig. 4). 3. So we are going to make a correction splint and rehabilitation set for facial palsy, that makes common use broadly in based of medical utility (CAD. 1~7). Conclusion : We invented a correction splint and rehabilitation set for facial palsied patients in based of questionnaire survey.

• Proceedings of the Korean Society of Computer Information Conference
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• 2012.07a
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• pp.75-77
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• 2012

최근 국내 과학기술분야 학회에서는 발행 학술지를 세계적인 학술지로 등재하고자 하는 노력이 증가하고 있다. 이에 따라, 해외연구자들을 학술지 편집위원으로 참여 시키고 많은 해외 연구자들이 자체 학술지에 논문을 많이 투고하도록 독려하고 있다. 해외 연구자들간에 이메일로 투고 심사를 처리하고 심사 총평을 작성하는 논문투고심사 프로세스는 심사 이력이 관리도 안 될 뿐만 아니라 논문투고 심사 진행 상황을 알 수 없음으로 인한 이용자의 불편함, 시간적 비효율성 등의 많은 단점이 있다. 이에 KISTI는 해외 연구자들이 학술지 생산을 위한 학술활동 전 과정에 참여할 수 있도록 하고자, 학술지를 체계적으로 운영하고 투명한 상호심사(Peer Review) 과정을 포함하는 학술지 전자 출판과정을 온라인으로 처리하고 모든 메뉴 용어를 국제적인 수준의 영어로 기술한 영문 Article Contribution Management System(ACOMS Ver. 4.0)를 개발하였다. 영문ACOMS는 기존 국문 ACOMS와 달리, 학술지 홈페이지를 포함하고 있으며, 저자와 편집위원 혹은 저자와 판사 간의 온라인 논문 교정 프로세스가 들어가 있다. 영문 ACOMS는 2012년 현재에도 국제적인 시스템으로 거듭나기 위해 지속적인 기능개선을 추진하고 있다. 개발된 영문 ACOMS 기능에 대해 살펴보고, 향후 개선방안에 대해 기술한다.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.6 no.2
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• pp.222-248
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• 1996

This study was designed to survey misused expression in 37 KS (Korean Industrial standards) related to personal protective equipment. The results were as follows: 1. One hundred and twenty five misused expressions were found - 44 expressions were used illogically (35.2 %). 80 expressions were wrongly described (64.0 %) The types of illogically misused are: 10 illogical cases, 4 cases of a wrongly designated provision when cited, 12 cases of the lack of provisons for KS, 7 cases of a inappropriate uses of units, 8 cases of an overlapped sizes between standards. - The types of expressions wrongly described are: 30 cases of confusion in using an terms, 4 case of making an error when proofreading, and 29 cases of choosing words not appropriate to describe technical standard, 18 cases of unclear provisons due to missing phrases and terms, and 11 cases of provisons described differently from original intended purposes. 2. There is one case, where the same standard is categorized as two different names. There are three major causes of the misused expressions as seen from the following summary: - Imitated the developed countries' standards without filtering them by considering domestic law and ocher KS standards. - Tended to create each definition for each standard instead use of the basic standard. - Insufficiency of synthetic examination to the correlation between standards.

• Journal of Microbiology and Biotechnology
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• v.15 no.6
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• pp.1359-1367
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• 2005

The gene encoding Pyrobaculum arsenaticum DNA polymerase (Par DNA polymerase) was cloned and sequenced. The gene consists of 2,361 bp coding for a protein with 786 amino acid residues. The deduced amino acid sequence of Par DNA polymerase showed a high similarity to archaeal family B-type DNA polymerases (Group I), and contained all of the motifs conserved in the family B-type DNA polymerases for $3'{\rightarrow}5'$ exonuclease and polymerase activities. The Par DNA polymerase gene was expressed under the control of the T7lac promoter on the expression vector pET-22b(+) in Escherichia coli BL21-CodonPlus(DE3)-RP. The expressed enzyme was purified by heat treatment, and Cibacron blue 3GA and $Hirap^{TM}$ Heparin HP column chromatographies. The optimum pH of the purified enzyme was 7.5. The enzyme activity was activated by divalent cations, and was inhibited by EDTA and monovalent cations. The half-life of the enzyme at $95^{\circ}C$ was 6 h. Par DNA polymerase possessed associated $3'{\rightarrow}5'$ proofreading exonuclease activity, which is consistent with its deduced amino acid sequence. PCR experiment with Par DNA polymerase showed an amplified product, indicating that this enzyme might be useful in DNA amplification and PCR-based applications.