• The Korean Journal of Physiology and Pharmacology
• /
• v.10 no.1
• /
• pp.51-58
• /
• 2006

The promoting effect of hydrogen peroxide ($H_2O_2$) against the cytotoxicity of 1-methyl-4-phenylpyridinium ($MPP^+$) in differentiated PC12 cells was assessed by measuring the effect on the mitochondrial membrane permeability. Treatment of PC12 cells with $MPP^+$ resulted in the nuclear damage, decrease in the mitochondrial transmembrane potential, cytosolic accumulation of cytochrome c, activation of caspase-3, increase in the formation of reactive oxygen species (ROS) and depletion of GSH. Addition of $H_2O_2$ enhanced the $MPP^+-induced$ nuclear damage and cell death. Catalase, Carboxy-PTIO, Mn-TBAP, N-acetylcysteine, cyclosporin A and trifluoperazine inhibited the cytotoxic effect of $MPP^+$ in the presence of $H_2O_2$. Addition of $H_2O_2$ promoted the change in the mitochondrial membrane permeability, ROS formation and decrease in GSH contents due to $MPP^+$ in PC12 cells. The results show that the $H_2O_2$ treatment promotes the cytotoxicity of $MPP^+$ against PC12 cells. $H_2O_2$ may enhance the $MPP^+$-induced viability loss in PC12 cells by promoting the mitochondrial membrane permeability change, release of cytochrome c and subsequent activation of caspase-3, which is associated with the increased formation of ROS and depletion of GSH. The findings suggest that $H_2O_2$ as a promoting agent for the formation of mitochondrial permeability transition may enhance the neuronal cell injury caused by neurotoxins.

• Research in Plant Disease
• /
• v.12 no.1
• /
• pp.46-50
• /
• 2006

The microorganisms with the antifungal activity against Phytophthora capsici and Colletotrichum acutatum and the plant growth-promoting activity were screened from a forest and natural fields of Gajang-Dong, Sangju-city. One of the isolates, Streptomyces griseofuscus 200401, was selected as a good plant growth-promoting strain in this study. In greenhouse test, the number of leaf, fresh weight, and dry weight of pepper plants, that were grown with treatment of culture suspension or powder containing S. griseofuscus 200401, were higher than those without the bacterial cells. Cultivation of S. griseofuscus 200401 strain for 7 days in a nutrient rich medium produced ammonium chloride up to 0.13 ${\mu}g/ml$ in the culture solution of S. griseofuscus. Treatment of the selected strain significantly reduced the severity of the late blight of pepper plants to show the equivalent disease control activity to chemical fungicide. This study suggests that S. griseofuscus 200401 strain could be a potential biological agent with the biocontrol activity and the plant growth-promoting activity.

• Korean Journal of Environmental Agriculture
• /
• v.20 no.1
• /
• pp.57-62
• /
• 2001

Twelve antagonistic strains against Fusarium wilt of watermelon-rootstock gourd were selected from 54 bacterial isolates which were isolated from the rhizosphere of crop plants growing in various locations. They showed strong inhibitory effects on growth of Fusarium osysporum f. sp. niveum, the causal agent of watermelon-rootstock gourd Fusarium wilt. Among these antagonists, the isolate YJ-3 was the most pronounced in growth-promoting ability for watermelon-rootstock gourd. The growth of watermelon-rootstock gourd in bed soil inoculated with YJ-3 was better by 46 and 13% than those in commercial bed soil alone and in bed soil inoculated with commercial microbial inoculant, respectively. The antagonistic plant growth-promoting rhizobacterium, strain No. YJ-3, was identified as Bacillus sp. on MIDI system. Furthermore, Bacillus sp. YJ-3 showed antifungal activity on growth against Alternaria cucumerina, Botrytis cinerea, Colletotrichum orbiculare, Didymella bryoniae, Rhizoctonia solani and Fusarium oxysporum.

• Korea Journal of Hospital Management
• /
• v.15 no.2
• /
• pp.44-60
• /
• 2010

This research, which is designed to introduce the concept of the WHO's health promoting hospital project to Korea, was conducted in a total of 34 local hospitals across the nation. To evaluate the level of health promotion at hospitals, an evaluation index for health promoting hospital environments was made using the Analytic Hierarchy Process Decision-Making Method, from which a total of 20 questions were developed in the five areas of no-smoking, moderation in drink, exercise, nutrition and rest in Korea. Through this analysis, it was found that local hospitals across the nation were on average excellent in terms of their no-smoking environments, but poor in their rest and moderation in drink environments. A comparison of local public hospital environments by region showed that Busan, Daegu, and South Gyeongsang Province were good, while South Chungcheng Province, Jeju Province and Gwangwon Province were poor. In terms of the number of beds, mid-size local hospitals (200-299 beds) came first. This research revealed that local hospitals across the nation had different health promotion environments according to area and size, and in particular, their environments for rest and moderation in drink turned out to be lacking, which vividly showed that these areas desperately needed to be supplemented in order to introduce the concept of health promotion at hospitals in Korea.

• Journal of the Korean Home Economics Association
• /
• v.46 no.3
• /
• pp.103-116
• /
• 2008

With increasing of communication technology in cyber-space, the space of consumer complain behavior moved from off-line to on-line, so called anti-site. No researches, however, really have done how the anti-sites worked for promoting consumer rights through consumer complain behaviors in cyber space. The purpose of this research was to give some suggestions for activating anti-site for promoting consumer rights. For this purpose, questionnaire on-line survey was conducted from April, 12 to 24, 2004. And 143 samples, who were visiting anti-site during survey period, were selected. Main findings were as following: First, visitors had rather positive confidence with informations in anti-sites. Second, visitors thought that anti-sites were working rather well in implementing consumer movement by sharing their experiences, and by supporting complaining space in cyber. Visitors, however, thought that anti-sites were not working well as a space for companies, not by giving their attentions on consumers' complains. Third, the satisfaction level of visitors was found to be rather high in terms of informations in anti-sites, and to be rather low in terms of companies' attentions on anti-sites and management of anti-sites. Based on results, some suggestions of activating anti-site for promoting consumer rights were as following: First, visitors should not only get information, but support new and useful informations for others. And also it was suggested that visitors should join consumer movement through anti-site actively. Second, companies should pay attentions on anti-sites for their sake because anti-sites could give very useful information improving customer satisfaction, and finally reducing cost and increasing profit.

• The Journal of Korean Academic Society of Nursing Education
• /
• v.7 no.2
• /
• pp.298-307
• /
• 2001

The purpose of this study was to test the effect of the health education on college students. The content of the health education was consisted of significance of health, smoking, alcohol use and sexuality, birth control, exercise and nutrition, chronic illness, contagious disease, stress management. Two groups non-equivalent to pre and post test quasi-experimental research design were used in this study. The total number of subjects were 174 college students who did not have a health related major. The experimental group attended this health education program for 16 weeks and the control group attended other liberal arts and science classes for 16 weeks. Data were analysed with the SPSS window program. The homogeneity between the experimental and the control group pretest data was tested by $X^2$ test and t-test. The differences in stress, self-esteem, general self-efficacy, locus of control and health promoting behaviors after the health education were tested with the repeated measure ANOVA. The results were as follows: 1. There was no significant difference in general characteristics, stress, self- esteem, general self-efficacy, locus of control and health promoting behaviors between the two groups before the health education. 2. There was a significant difference in stress(p= .000) and health promoting behavior (p= .000) between the two groups after the health education. 3. There was no significant difference in self-esteem(p= .531), self-efficacy(p= .110) and locus of control between the two groups after the health education. From the results above, it can be concluded that this health education for the college students was effective to relieve stress and improve health promoting behaviors. The above results suggest that repeated studies are needed for another college students and various health education programs should be developed to promote the health of the populace considering many health related concepts.

• Journal of Microbiology and Biotechnology
• /
• v.28 no.6
• /
• pp.968-975
• /
• 2018

In the course of screening for microbes with nematicidal activity, we found that Enterobacter asburiae HK169 displayed promising nematicidal activity against the root-knot nematode Meloidogyne incognita, along with plant growth-promoting properties. Soil drenching of a culture of HK169 reduced gall formation by 66% while also increasing root and shoot weights by 251% and 160%, respectively, compared with an untreated control. The cell-free culture filtrate of the HK169 culture killed all juveniles of M. incognita within 48 h. In addition, the nematicidal activity of the culture filtrate was dramatically reduced by a protease inhibitor, suggesting that proteolytic enzymes contribute to the nematicidal activity of HK169. In order to obtain genomic information about the HK169 isolate related to its nematicidal and plant growth-promoting activities, we sequenced and analyzed the whole genome of the HK169 isolate, and the resulting information provided evidence that the HK169 isolate has nematicidal and plant growth-promoting activities. Taken together, these observations enable the future application of E. asburiae HK169 as a biocontrol agent for nematode control and promote our understanding of the beneficial interactions between E. asburiae HK169 and plants.

• Research in Community and Public Health Nursing
• /
• v.14 no.1
• /
• pp.66-74
• /
• 2003

Purpose: This study was conducted to identify the difference of the health promoting behaviors between average Internet users and excessive Internet users. Method: Data were collected from a convenient sample of 465 middle school students from June 7th to 17th, 2002, based on a self reported questionnaire. The instruments included were the Health Promotion Behaviors reconstructed by the author based on the Health Promoting Lifestyle Profile (Walker, Sechrist & Pender, 1987) and Internet addiction test translated by Center for Internet Addiction Prevention and Counseling based on Young's test. Data were analyzed with N, %, x2 test, t-test and Pearson's correlation coefficient. Result : 1. There were no significant differences in general characteristics between average Internet users and excessive Internet users. 2. There were significant differences between two groups in diet behavior(p=.030), even though there were no differences in general health promoting behaviors(p=.109). 3. There were significant negative correlations between Internet use and diet behavior (r=-.193, p=.000). Therefore, average Internet users had more desirable diet behavior than excessive Internet users. Conclusion: Internet addiction prevention program should be conducted as part of a comprehensive school health promotion program. In addition, the results of this study should be considered in developing the school health education curriculum to rear students' responsibility on their health behaviors.

• Korean Journal of Health Education and Promotion
• /
• v.26 no.4
• /
• pp.49-62
• /
• 2009

Objectives: Few studies have focused on health behaviors and health status among teachers even though these are one of principles and values of WHO's Health Promoting Schools. In this study, we described health behaviors and health status among teachers and explored the differences of these by personal and school characteristics. Methods: A stratified and random cluster sampling design was used to obtain a cross-sectional sample. A total of 59 elementary, middle and high schools in Seoul and Gyeonggi areas were included in which 333 teachers (males 40.6%, females 59.4%) participated in this survey. Chi-square tests were conducted with SPSS 17.1. Results: Overall, health behaviors and health status among teachers were better than the general public according to Korea National Health and Nutrition Examination Survey. Male and middle/high school teachers had higher prevalence of alcohol dependence and of driving under the influence of alcohol. Also, their rate of wearing seat belts were lower compare to female. In other hand, female, health and nutrition teachers, rural school teachers had lower prevalence of meeting recommended levels of physical activity. Underweight were more prevalent to them in contrast to male with higher prevalence of obesity. In addition, teachers of health promoting schools were not likely to ride on a car with a drunk driver. They also have a lower prevalence of skipping breakfast and have higher positive perception of health for themselves. Conclusion: Teachers' health should be given higher concerns as a key component of school based health promotion. Gender and age specific programs should be considered to develop health promoting programs for them.

• Journal of Haehwa Medicine
• /
• v.9 no.1
• /
• pp.215-223
• /
• 2000

To understand immunopharmacologic effects on Cervi Pantotrichum Cornu, Morindae Officinalis Radix, Cistanches Herba, Curculginis Rhizoma, Epimedii Herba, Eucommiae Cortex, we investigated chinese experimental documents, and we could reach conclusions as follows : 1. The effects on cell-mediated immune system were as follows. 1) The effects on macrophage (1) The herbal medicines promoting to increase the number of WBC in the peripheral blood were Morindae Officinalis Radix, Epimedii Herba and that promoting to reinforce the phagocytic functions of neutrophil was Curculginis Rhizoma. (2) The herbal medicines promoting the phagocytic functions of mononuclear, macrophage were Cervi Pantotrichum Cornu, Cistanches Herba, Eucommiae Cortex. 2) The herbal medicines stimulating the activities of T lymphocytes were Cervi Pantotrichum Cornu, Curculginis Rhizoma, Epimedii Herba, Eucommiae Cortex. 2. The effects on humoral immune system were as follows. 1) The herbal medicines increasing the activity of complement receptor were Cervi Pantotrichum Cornu, Curculginis Rhizoma. 2) The herbal medicines reinforcing immunity of spleen cells were Cervi Pantotrichum Cornu, Cistanches Herba, Epimedii Herba. 3) The herbal medicines promoting proliferation of spleen cells that produce antibody after having been immunized by SRBC were Cervi Pantotrichum Cornu, Cistanches Herba, Epimedii Herba. 3. The herbal medicines, reinforcing immunity on delayed type hypersensitivity were Cervi Pantotrichum Cornu, Cistanches Herba, Eucommiae Cortex. As you know in the many bibliological documents, the studies on the effects of Drugs for Tonifying Yang were started along right lines. Recently the studies on those were accomplished more rapidly and applied many immune diseases. We thought that Drugs for Tonifying Yang could be important immunopotentiators. Therefore we can apply those herbal medicines not only to immune diseases but also inflammatory diseases, senile infirmity and all sorts of tumor.