• Asian Pacific Journal of Cancer Prevention
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• v.17 no.5
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• pp.2519-2525
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• 2016

There are several existing reports of microarray chip use for assessment of altered gene expression in different diseases. In fact, there have been over 1.5 million assays of this kind performed over the last twenty years, which have influenced clinical and translational research studies. The most commonly used DNA microarray platforms are Affymetrix GeneChip and Quality Control Software along with their GeneChip Probe Arrays. These chips are created using several quality controls to confirm the success of each assay, but their actual impact on gene expression profiles had not been previously analyzed until the appearance of several bioinformatics tools for this purpose. We here performed a data mining analysis, in this case specifically focused on ovarian cancer, as well as healthy ovarian tissue and ovarian cell lines, in order to confirm quality control results and associated variation in gene expression profiles. The microarray data used in our research were downloaded from ArrayExpress and Gene Expression Omnibus (GEO) and analyzed with Expression Console Software using RMA, MAS5 and Plier algorithms. The gene expression profiles were obtained using Partek Genomics Suite v6.6 and data were visualized using principal component analysis, heat map, and Venn diagrams. Microarray quality control analysis showed that roughly 40% of the microarray files were false negative, demonstrating over- and under-estimation of expressed genes. Additionally, we confirmed the results performing second analysis using independent samples. About 70% of the significant expressed genes were correlated in both analyses. These results demonstrate the importance of appropriate microarray processing to obtain a reliable gene expression profile.

• Journal of Life Science
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• v.15 no.5 s.72
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• pp.763-766
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• 2005

The purpose of this study was to investigate effects of regular exercise on ADL (activities of daily living) and lipid profiles (total cholesterol, triglyceride, HDL-C, LDL-C) in male patients with dementia. The subjects were consisted of 24 male patients with dementia, they were divided into two groups. the exercise group (EC, n=12) and the control group (CG, n=12). The exercise group participated in regular exercise program, and their ADL, lipid profiles levels were evaluated at baseline (pre, 0 mo), after 6 months (mid, 6 mo), and after 12 months (post, 12 mo). The subjects carried on exercising $30\∼60$ minutes a day, $2\∼3$ times per week for 12 months. Statistical techniques for data analysis was paired samples t-test. The level of statistical significance was $p\leq.0.5$. The results of this study were summarized as follows: In the case of EG, mid and post ADL values significantly higher than that of pre value, whereas there was no significant difference in the CG. Mid and post values of TC, TG, and LDL-C were significantly lower than that of pre value in the EG. However, mid and post HDL-C values were higher in the EG compared to pre value. In conclusion, these results suggest that regular exercise have an positive effect on ADL and lipid profiles in male patient with dementia. In addition, regular exercise may be helpful to reduce the incidence of heart disease and coronary sclerosis.

• The Korean Journal of Community Living Science
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• v.19 no.1
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• pp.113-123
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• 2008

This study was performed to find and compare the characteristics of dietary factors and obesity indices on serum lipid profiles using 407 healthy adult males that had physical examinations conducted at the general hospital located in Suwon. The subjects were divided 56.3% for the normal group, 16.7% for TG, 14.5% for TC and 12.5% for TC TG group, respectively. When comparing the blood lipid profiles to normal group, TC group was high in both total cholesterol and LDL-cholesterol, TG group was high in triglyceride and low in HDL-cholesterol, TC TG group was high in triglyceride, total cholesterol, LDL-cholesterol and low in HDL-cholesterol. In comparison of blood lipid profiles with normal group, among anthropometric factors, body weigh, BMI (Body Mass Index) and WHR (Waist Hip Ratio) showed significant differences. TC group was high in both total cholesterol and LDL-cholesterol, and TG group was high in triglyceride and low in HDL-cholesterol. TC TG group was high in triglyceride, total cholesterol and LDL-cholesterol, and was low in HDL-cholesterol. Body weigh of TG group was significantly higher than that of normal group (p<0.05). BMI and WHR of TG group and TC TG group were significantly higher than those of normal group (p<0.05). Total cholesterol was mainly related to the intake of animal lipids and alcoholic beverage. Serum triglyceride levels were closely related with lipid intake and high BMI in this group. In TC TG group, BMI and WHR were high. As well, serum LDL-cholesterol was high and HDL-cholesterol was low which indicates TC TG group had the most undesirable blood lipid profiles. Therefore, it may be necessary to manage BMI and WHR to prevent hyperlipidemia and obesity for adult males in Suwon.

• Korean Journal of Community Nutrition
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• v.10 no.5
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• pp.633-644
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• 2005

The body mass index, blood pressures and blood profiles of adults, who worked at three colleges in the Daejeon area, were evaluated for use in developing well-balanced menus at their workplace cafeterias and/or in creating nutrition education programs tailored to their health statuses. The data analyzed were from physical examinations performed in 2002 and in 2004 by the National Health Insurance Corporation. Overall, levels of systolic and diastolic blood pressure and serum cholesterol were lower in the 2004 samples than in those of 2002, but most samples in 2004 showed higher levels of SGOT. In 2002, statistical differences occured in the levels of blood pressure and serum cholesterol among age groups, but not in 2004. And in 2004, the older the age group, the higher the BMI. Among subjects in their twenties, three variables (systolic and diastolic blood pressure, and serum cholesterol) showed statistical differences; this increased to four variables (systolic and diastolic blood pressure, blood glucose and serum cholesterol) among subjects in their thirties and to six variables (Hb, SGOT, systolic and diastolic blood pressure, blood glucose and serum cholesterol) among those in their forties, followed by a decline to four variables (systolic and diastolic blood pressure, Hb and serum cholesterol) among subjects in their fifties. In general, the results of physical examinations showed improvements in blood pressure and blood profiles in 2004 in comparison with results in 2002. The level of SGOT was the exception. Despite these improvements, of those employees who had physical examinations in 2004, $5.2\%$ had high blood glucose and $18.1\%$ hypertension, $27.9\%$ hypercholesterolemia, $8.3\%$ registered abnormal liver functions, and $31.3\%$ were classified as overweight and obese. Moreover, because some vulnerable blood profiles were found in the older age groups, various programs, including nutrition education, and/or development of healthful diets as well as excercise activities, may be needed within their workplaces.

• Journal of Pharmaceutical Investigation
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• v.33 no.3
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• pp.245-253
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• 2003

Dissolution profile comparsions can be done by virtue of the similarity factor $(f_2)$. It is a logarithmic reciprocal square root transformation of the sum of squared error of % dissolution differences between two profiles at several time points. It gives information on the degree of similarity between the two profiles: An $f_2$ value between 50 and 100 suggests the similarity/equivalence of the two dissolution curves being compared. The objective of this report was to provide a careful examination on the $f_2$ metrics in detail. It was shown that $f_2$ values exceeded 50, when relative differences in % dissolved between two products were less than 15% at all time points. The similarity factor value was also found to be greater than 50, in cases when absolute % dissolution differences were below 10% at all time points. Interestingly, the $f_2$ value was changed by the number of the time points selected for calculation. In particular, $f_2$ tended to have higher values, when the $f_2$ metrics used a large number of time points in which % dissolved reached plateau. Finally, since the similarity factor was a sample statistics, it was impossible to infer type I/II errors and sampling error. Despite certain limitations inherited in the $f_2$ metrics, it was easy and convenient to evaluate how similar the two dissolution profiles were.

• Journal of Nutrition and Health
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• v.37 no.10
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• pp.888-898
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• 2004

This study was designed to investigate the effects of 8-week resistance exercise by using dumbell on the body com-position, serum hormone profiles and feeding patterns in young and healthy female students with over 30% of body fat, aged 22 years in Masan, Korea. The subjects were all six, performed successfully the entire exercise-program. Anthropometry, serum lipid and neurohormone profiles of subjects were analyzed at 0, 4 and 8 weeks after exercise. And also their food and nutrient intakes were self-reported by 3-day record and food frequency questionnaire. Triceps skinfold-thickness of the subject students significantly reduced after the exercise to 46% less than before (p < 0.002) . The serum concentrations of leptin, insulin and catecholamine appeared to decrease steadily, but not significantly. Total protein in serum increased significantly (p<0.05), and Na (p<0.005), K (p<0.001) and Fe (p<0.058) concentrations increased remarkably within the normal range. The 8-week resistance exercise tended to induce fiber consumption (p<0.089) of subject students and changed the P/M/S ratio changed into the desirable way from 0.8 : 1 : 1 to 1 : 1 : 1, and that of n-6/n-3 PUPA from 11 : 1 to 6 : 1. After taking exercise, the intake frequency of cookies (p<0.058) and raymeun (p<0.085) decreased, but finally this feeding pattern returned to the way as before even though at marginal significance. Consequently the resistance exercise may induce the desirable changes of body fat and improve serum lipid profiles and feeding patterns in young obese females.

• Journal of the Korean Society for Nondestructive Testing
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• v.31 no.5
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• pp.479-486
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• 2011

In image-based CFD modeling of carotid bifurcation hemodynamics, it is often not possible (or at least not convenient) to impose measured velocity profiles at the common carotid artery inlet. Instead, fully-developed velocity profiles are usually imposed based on measured flow rates. However, some studies reported a pronounced influence of inflow boundary conditions that were based on actual velocity profiles measured by magnetic resonance imaging which showing the unusual presence of a high velocity band in the middle of the vessel during early diastole inconsistent with a Dean-type velocity profile. We demonstrated that those velocity profiles were induced by the presence of modest secondary curvature of the inlet and set about to test whether such more "realistic" velocity profiles might indeed have a more pronounced influence on the carotid bifurcation hemodynamics. We found that inlet boundary condition with axisymmetric fully-developed velocity profile(Womersley flow) is reasonable as long as sufficient CCA inlet length of realistic geometry is applied.

• Journal of Nutrition and Health
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• v.38 no.1
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• pp.11-19
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• 2005

There has been increasing research interests that green vegetables play beneficial roles in human health. This study was performed to investigate the effects of freeze-dried green vegetable extract of Angelica keiskei Koidz (A) and Brassica oleracea acephala (B) on lipid profiles and antioxidant status in rats. Seven-weeks old male Sprague Dawley rats were divided into 6 groups and fed diets containing 5% A & B and 0.5% cholesterol (cho) for 8 weeks [Control Diet (C) & C + chol (CC), A & A + chol (AC), B & B + chol (BC)]. Lipid profiles and antioxidant status were determined by enzyme assay methods. The serum levels of [LDL + VLDLJ-cholesterol of the rats fed vegetable extract diets A and B were significantly lower than that of group C and the ratios of HDL/[LDL + VLDL] were significantly higher in groups A and B. Addition of cholesterol in the diet, however, abolished this effect. The Brassica oleracea acephala juice lowered serum TG level even when cholesterol was added to the diet. Serum total antioxidant status(TAS) were significantly higher in groups A and B as compared to the control group and the ratios of [GSH-Px +Catalase]/total-SOD in the liver were also significantly higher in groups A and B indicating that H202 produced be efficiently removed. In conclusion, freeze-dried green vegetable extract diets (A and B) improved serum lipid profiles by increasing the HDL/[LDL + VLDL〕 ratio and exerted favorable influences on antioxidant systems by improving total antioxidant status (TAS) in serum and by significantly increasing the ratio of [GSH-Px + Catalase]/total-SOD in the liver.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.10
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• pp.4217-4224
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• 2014

Cholangiocarcinoma (CCA), a slow growing but highly metastatic tumor, is highly prevalent in Northeast Thailand. Specific tests that predict prognosis of CCA remain elusive. The present study was designed to investigate whether peripheral blood leukocyte (PBL) transcriptional profiles might be of use as a prognostic test in CCA patients. Gene expression profiles of PBLs from 9 CCA and 8 healthy subjects were conducted using the Affymetrix HG_U133 Plus 2.0 GeneChip. We indentified informative PBLs gene expression profiles that could reliably distinguish CCA patients from healthy subjects. Of these CCA specific genes, 117 genes were up regulated and 60 were down regulated. The molecular and cellular functions predicted for these CCA specific genes according to the Gene Ontology database indicated differential PBL expression of host immune response and tumor progression genes (EREG, TGF ${\beta}1$, CXCL2, CXCL3, IL-8, and VEGFA). The expression levels of 9 differentially expressed genes were verified in 36 CCA vs 20 healthy subjects. A set of three tumor invasion related genes (PLAU, CTSL and SERPINB2) computed as "prognostic index" was found to be an independent and statistically significant predictor for CCA patient survival. The present study shows that CCA PBLs may serve as disease predictive clinically accessible surrogates for indentifying expressed genes reflective of CCA disease severity.

• Journal of the Korean Geographical Society
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• v.49 no.3
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• pp.339-356
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• 2014

Easy access to DEMs and the development of technology treating DEMs make it easier to extract stream longitudinal profiles from DEMs than previously done. Since such profiles possess many problems such as artificial flats and steps, it should be required for them to be smoothed like natural profiles to estimate gradient values along those sections. However smoothing itself comes with much distortion of raw profile from original DEMs. There has been no research evaluating quantitatively the effects due to smoothing process. Here we attempt to quantify the effects of major smoothing methods on raw and real profiles, suggest a new method to overcome the limitations of them, and evaluate it. This study not only suggests a new smoothing method, but also provides a guideline for choosing a proper smoothing method.