• Animal Bioscience
• /
• v.35 no.4
• /
• pp.587-595
• /
• 2022

Objective: The present study was conducted to evaluate the effects of dietary supplementation with Bifidobacterium animalis, Agave fructans, and symbiotic of both encapsulated on growth performance, feed efficiency, blood parameters, and immune status in broiler chickens, and to compare these with diets including antibiotic growth promoters and without additives. Methods: A comparative experimental study was carried out with 135 male Ross 308 broiler chickens. Each trial was divided into 5 equal groups. Control group (CON) received a standard diet without growth promoter; GPA, a standard diet with colistin sulfate and zinc bacitracin (0.25 g/kg of feed); PRE, a standard diet with 1% Agave fructans; PRO, a standard diet with Bifidobacterium animalis (11.14±0.70 log CFU/g); SYM, a standard diet with B. animalis and Agave fructans. Results: A significant decrease in food consumption was found for the GPA, PRE, and SYM, compared to the CON group. The results show a better feed conversion index in PRE and GPA with respect to the CON group with the highest conversion index. Interestingly, the weight of the gastrointestinal tract shows a statistically significant difference between GPA and PRE groups. Moreover, the length of the gastrointestinal tract of the GPA group was less than the PRE group. In the total leukocyte count, there was a statistically significant increase in the GPA group compared to the CON, PRE, and PRO groups, and the heterophiles-lymphocytes index was lower in PRO. Regarding the cytokines, interleukin 10 (IL-10) decreased in PRO compared to CON and PRE, while IL-1β increased in the SYM group. Conclusion: Alternative treatments were shown to achieve similar productive results as growth-promoting antibiotics and showed improvement over diet without additives; however, they have immunomodulatory properties and improved the development of the gastrointestinal tract compared to the treatment of growth-promoting antibiotics.

• Korean Journal of Soil Science and Fertilizer
• /
• v.18 no.2
• /
• pp.156-160
• /
• 1985

24 cultivation areas at fields of the Tobacco Experiment Station in Suweon, Eumseong, and Daegu were selected to get the fertilizer response and evaluate fertility level of upland soil. Fertilizer levels treated were 0, 70, 100, and 130kg/10a of compound fertilizer (10-10-20) for tobacco. The results are as follows; 1. Fertility level could be evaluated by dry weight of tobacco loaves harvested from non-fertilized fields. 2. There was significant in yield differences between fertilizer levels of 70 and 100kg/10a but was no difference between 100 and 130kg/10a of fertilizer level. The lower fertilizer efficiencies were showed at the fields harvested high yield of tobacco leaf without fertilizer. 3. Optimum fertilizer level for non-fertilized field with leaf yield less than 208.5kg/10a was 100kg/10a, and at low productive field with dry leaf weight less than 33.3kg/10a, higher level of fertilizer, 130kg/10a, was considered to be the optimum to get the got income with tobacco cultivation.

• Journal of English Language & Literature
• /
• v.57 no.6
• /
• pp.977-998
• /
• 2011

The purpose of this study is to investigate prospective teachers' perceptions of the peer review comments readily available to them during the writing process in a teacher training class. Given these needs, we employ a qualitative method of inquiry giving voice to the learner's own view of peer feedback. The data we wish to consider is first-person narratives elicited from four EFL college students, who are prospective teachers of English. With regard to the EFL students' narrative considered here, all were attentive to the feedback they received. Moreover, the way in which these EFL writers talk about peer response activity reflects that they still welcome peer feedback because of the benefits to be accrued from it. Although this study, covering only four EFL students in total, can hardly be considered conclusive, we attempt to offer a synthesis of their stories. First of all, students indicate that they received responses from "authentic readers" (Mittan 1989, 209). We do note, consequently, that students gain a clear understanding of readers' needs by receiving feedback on what they did well and on what seems unclear. Perhaps the greater effect of peer feedback claimed by these students is that they take active roles in utilizing peer comments. Since they feel uncertain about the validity of their classmates' responses, students feel that they have autonomy over their own text and can make their own decisions on whether they should accept their peer comments or not. This contrasts with their treatment of teacher comments that they accept begrudgingly even if they disagree with them. Four EFL writers talked a lot, typically in a positive way, about peer response to their writing, yet they have expressed reservations about the extent to which they should put any credence in comments offered by their fellow students. Perhaps this is because their fellow students are still developing writers and EFL learners. In turn, they were sometimes reluctant to accept the peers' comments. Thus, in EFL contexts, L1 use can be suggested during peer feedback sessions. In particular, we have come to feel that L1 use enables both reviewers and receivers to have more productive peer review experiences. Additionally, we need to train students not "to see peer feedback as potentially bad advice" (Silva et al. 2003, 111). Teachers should focus on training students to utilize their peers' comments. Without such training, students will either ignore feedback or fail to use it constructively.

• Korean Journal of Poultry Science
• /
• v.41 no.4
• /
• pp.323-329
• /
• 2014

The objective of this experiment was to investigate the effect of dietary supplementation of copper-soy proteinate (Cu-SP) and herbal mixture (HBM) on growth performance, blood parameter, and immune response in laying hens. A total 800 Hy-Line Brown laying hens (60 weeks old) were randomly allotted to 1 of 4 dietary treatments : (1) Control : control diet, (2) Cu-SP : control diet + 100 mg/kg Cu-soy proteinate, (3) HBM : control diet + 0.15% herbal mixture, and (4) Cu-SP + HBM : control diet + 100 mg/kg Cu-soy proteinate + 0.15% herbal mixture. Each treatment was replicated 5 times with forty birds units were arranged according to randomized block design. Feeding trial lasted 5 weeks under 16L : 8D lighting regimen. The diet and water were available ad libitum. Result indicated that during feeding trial of the experiment, hen-day egg production was significantly (P<0.05) higher in Cu-SP and HBM treated groups than control. However, feed intake, feed conversion ratio, egg weight, broken and shell less egg production were not significantly influenced by treatments. Eggshell strength was significantly (P<0.05) higher in Cu-SP than control. Eggshell thickness, eggshell color, egg yolk color, Haugh unit were not significantly influenced by treatments. The level of WBC and stress index (heterophil : lymphocyte) were higher in supplemented groups than the control. The concentration of plasma IgG was higher in supplemented groups than the control. The result of this experiment showed that dietary copper-soy proteinate or herbal mixture tended to improve egg production and affect positively on immune response of laying hens.

• Korean Journal of Plant Resources
• /
• v.36 no.2
• /
• pp.172-180
• /
• 2023

In the current study, four groups; control, 500, 1000, and 2000 mg/L, were treated to investigate the effects of physiological and growth characteristics on Machilus thunbergii under various fertilization levels. As a result of the physiological response to the fertilization treatment, the fertilized group demonstrated relatively higher levels of A, ITE, WUEi, V_camx, PI_abs, and SFI_abs in comparison to the control. The best photosynthetic mechanism was most clearly seen at 1000 mg/L, which involved gas exchange through active stomatal opening and closing. For a productive photosynthetic mechanism. As seen in the growth response of M. thunbergii to fertilization treatment, the fertilized group has significantly higher height, DRC, leaf dry weight, stem dry weight, total dry weight, LWR, and SWR than the control group. A healthy seedling quality index was particularly evident at 1000 mg/L, and other growth indicators were also at a decent level. 500 mg /L also demonstrated growth characteristics that were comparable to those at 1000 mg/L. As a result, M. thunbergii featured the best physiological and growth characteristics in response to the fertilization treatment at 1000 mg/L, and 500 mg/L also showed a similar trend, which is considered to be a good option from an economical perspective.

• Asian-Australasian Journal of Animal Sciences
• /
• v.27 no.1
• /
• pp.55-61
• /
• 2014

Forty-eight Pelibuey${\times}$Katahdin male intact lambs ($23.87{\pm}2.84$ kg) were used in an 84-d feeding trial, with six pens per treatment in a $2{\times}2$ factorial design arrangement. The aim of the study was to evaluate the interaction of two dietary energy levels (3.05 and 2.83 Mcal/kg ME) and two dietary protein levels (17.5% and 14.5%) on growth performance, dietary energetics and carcass traits. The dietary treatments used were: i) High protein-high energy (HP-HE); ii) High protein-low energy (HP-LE); iii) Low protein-high energy (LP-HE), and iv) Low protein-low energy (LP-LE). With a high-energy level, dry matter intake (DMI) values were 6.1% lower in the low-protein diets, while with low-energy, the DMI values did not differ between the dietary protein levels. Energy levels did not influence the final weight and average daily gain (ADG), but resulted in lower DMI values and higher gain efficiencies. No effects of protein level were detected on growth performance. The observed dietary net energy (NE) ratio and observed DMI were closer than expected in all treatments and were not affected by the different treatments. There was an interaction (p<0.03) between energy and protein level for kidney-pelvic and heart fat (KPH), KPH was higher in lambs fed high energy and high protein diet but not in high energy and low protein diet. The KPH was increased (20.2%, p = 0.01) in high-energy diets, while fat thickness was increased (21.7%, p = 0.02) in high-protein diets. Therefore, it is concluded that dietary energy levels play a more important role in feed efficiency than protein levels in finishing lambs with a high-energy diet (>2.80 Mcal/kg ME). Providing a level of protein above 14.5% does not improves growth-performance, dietary energetics or carcass dressing percentage.

• Journal of Digital Convergence
• /
• v.10 no.3
• /
• pp.241-250
• /
• 2012

Low birth rate is a persisting national challenge that causes a decrease in productive population and an increase in encumbrances by ever rising insurance premiums, eventually threatening the existence of the country. This study investigated the effectiveness of the current government's undergoing economic support of the child-birth promotion policy, "go-un-mam card", and suggested improvement plans about the problems derived on the basis of the perceptions of the card users. Multi-response analysis was used to find out the preference of the policy, and chi-square test was conducted to discover the user satisfaction rate and intent of re-parturition. Also, descriptive analysis was performed to identify the degree of the policy recognition. The results illustrated that a significant association exists between the satisfaction rate and the intention of re-parturition. In addition, pregnant women gained information about the policy from governmental agencies and medical institution as well as by word of mouth; then, applied to the policy. Also, the card users only took an advantage of discounts in the hospitals within the supported monetary amounts. Moreover, the card users expressed their dissatisfaction at the monetary amounts. For instance, the users were dissatisfied with the limit on the amount to be used in a day and requested upgrade on the monetary amounts. Based on the result, the government will improve and develop the go-un-mam card for the ultimate purpose of policy, increasing birth-rate.

• Asian-Australasian Journal of Animal Sciences
• /
• v.14 no.6
• /
• pp.880-884
• /
• 2001

Rumen microbiology research has undergone several evolutionary steps: the isolation and nutritional characterization of readily cultivated microbes; followed by the cloning and sequence analysis of individual genes relevant to key digestive processes; through to the use of small subunit ribosomal RNA (SSU rRNA) sequences for a cultivation-independent examination of microbial diversity. Our knowledge of rumen microbiology has expanded as a result, but the translation of this information into productive alterations of ruminal function has been rather limited. For instance, the cloning and characterization of cellulase genes in Escherichia coli has yielded some valuable information about this complex enzyme system in ruminal bacteria. SSU rRNA analyses have also confirmed that a considerable amount of the microbial diversity in the rumen is not represented in existing culture collections. However, we still have little idea of whether the key, and potentially rate-limiting, gene products and (or) microbial interactions have been identified. Technologies allowing high throughput nucleotide and protein sequence analysis have led to the emergence of two new fields of investigation, genomics and proteomics. Both disciplines can be further subdivided into functional and comparative lines of investigation. The massive accumulation of microbial DNA and protein sequence data, including complete genome sequences, is revolutionizing the way we examine microbial physiology and diversity. We describe here some examples of our use of genomics- and proteomics-based methods, to analyze the cellulase system of Ruminococcus flavefaciens FD-1 and explore the genome of Ruminococcus albus 8. At Illinois, we are using bacterial artificial chromosome (BAC) vectors to create libraries containing large (>75 kbases), contiguous segments of DNA from R. flavefaciens FD-1. Considering that every bacterium is not a candidate for whole genome sequencing, BAC libraries offer an attractive, alternative method to perform physical and functional analyses of a bacterium's genome. Our first plan is to use these BAC clones to determine whether or not cellulases and accessory genes in R. flavefaciens exist in clusters of orthologous genes (COGs). Proteomics is also being used to complement the BAC library/DNA sequencing approach. Proteins differentially expressed in response to carbon source are being identified by 2-D SDS-PAGE, followed by in-gel-digests and peptide mass mapping by MALDI-TOF Mass Spectrometry, as well as peptide sequencing by Edman degradation. At Ohio State, we have used a combination of functional proteomics, mutational analysis and differential display RT-PCR to obtain evidence suggesting that in addition to a cellulosome-like mechanism, R. albus 8 possesses other mechanisms for adhesion to plant surfaces. Genome walking on either side of these differentially expressed transcripts has also resulted in two interesting observations: i) a relatively large number of genes with no matches in the current databases and; ii) the identification of genes with a high level of sequence identity to those identified, until now, in the archaebacteria. Genomics and proteomics will also accelerate our understanding of microbial interactions, and allow a greater degree of in situ analyses in the future. The challenge is to utilize genomics and proteomics to improve our fundamental understanding of microbial physiology, diversity and ecology, and overcome constraints to ruminal function.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.17 no.1
• /
• pp.1-6
• /
• 2011

The underlying principle of environmental conservation and restoration is to receive continuous services from nature. In this context, the basic objective of oil spill response and recovery activities is to allow affected residents to retain sustainability of their daily livelihood and productive environment. Hence, monitoring the status of socio-economic impact and the extent of restoration is an important parameter in the restoration effort to repair the damaged functions of environmental services with oil spill accident. However, assessment of socio-economic impact poses investigators with much difficulties in selecting the most appropriate approaches because the process often involves various stakeholders, directly and indirectly. Moreover, the analyses and interpretation of the results also pose a great challenge. The present study monitored fisheries and tourism numbers which were considered as major local socio-economic indicators of living and production environment affected by M/T Hebei Spirit oil-spill accident. This monitoring was conducted by examining the published papers and statistical reports. This was supplemented by surveying how the local residents actually felt about the damage and recovery for the first time in Korea. The results showed that the rate of the recovery was about 40~50 %, and the rate of the recovery seemed to be slowed or decreased. However, what the local residents actually felt was 2~10 points less than the literature surveys and statistical reports. These results suggested limits to using only the literature and statistical surveys for the traditional socio-economic impact assessment. The study also showed the need to include in the impact assessment process what and how the local residents actual feel about the oil spill damage and recovery process.

• Journal of The Korean Dental Society of Anesthesiology
• /
• v.14 no.3
• /
• pp.157-165
• /
• 2014

Background: Incisional site of surgical operation become transient ischemic state and then occur reoxygenation due to vasodilatation by inflammatory reaction, the productive reactive oxygen species (ROS) give rise to many physiologic results. Apoptosis have major role on elimination of inflammatory cell and formation of granulation tissue in normal wound healing process. Remifentanil can prevent the inflammatory response and can suppress inducible nitric oxide synthase expression in a septic mouse model. After cardiopulmonary bypass for coronary artery surgery, remifentanil can also inhibit the release of biomarkers of myocardial damage. Here we investigated whether remifentanil pretreatment has cellular protective effect against hypoxia-reoxygenation in HaCaT human keratinocytes, if so, the role of apoptosis and autophagy on this phenomenon. Methods: The HaCaT human keratinocytes were exposed to various concentrations of remifentanil (0.01, 0.05, 0.1, 0.5 and 1 ng/ml) for 2 h before hypoxia (RPC/HR group). These cells were cultured under 1% oxygen tension for 24h at $37^{\circ}C$. After hypoxia, to simulate reoxygenation and recovery, the cells were reoxygenated for 12 h at $37^{\circ}C$. 3-MA/RPC/HR group was treated 3-methyladenine (3-MA), autophagy inhibitor for 1h before remifentanil treatment. Cell viability was measured using a quantitative colorimetric assay with thiazolyl blue tetrazoliumbromide (MTT, amresco), showing the mitochondrial activity of living cells. To investigate whether the occurrence of autophagy and apoptosis, we used fluorescence microscopy and Western blot analysis. Results: The viability against hypoxia-reoxygenation injury in remifentanil preconditioning keratinocytes were increased, and these cells were showed stimulated expression of autophagy 3-MA suppressed the induction of autophagy effectively and the protective effects on apoptosis. Atg5, Beclin-1, LC3-II and p62 were elevated in RPC/HR group. But they were decreased when autophagy was suppressed by 3-MA. Conclusions: Remifentanil preconditioning showed the protective effect in human keratinocytes, and we concluded that autophagy may take the major role in the recovery of wound from hypoxia-reoxygenation injury. We suggest that further research is needed about the cell protective effects of autophagy.