• Korean Journal of Medicinal Crop Science
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• v.13 no.2
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• pp.91-94
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• 2005

Plants have been known to accumulate a very diverse range of triterpene saponins. We have investigated the regulation of saponin biosynthesis in higher plants using Centella asiatica (L.) Urban as a model plant. Effects of a feeding precursor on asiaticoside production from leaves and on the level of two-type OSCs mRNA were investigated. As a feeding precursor, squalene negatively affected the levels of CYS and bAS mRNA, but it also decreased the production of asiaticoside from whole plants. Plant hormones regulate secondary metabolism, and in plant tissue cultures they could affect both culture growth and secondary metabolite production. Although enhancement of asiaticoside production from whole plant cultures by addition of TDZ (thidiazuron) has been reported, the positive effect of TDZ on the levels of OSCs transcripts was not observed.

• Journal of Computational Design and Engineering
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• v.1 no.3
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• pp.173-186
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• 2014

The failure of a subsea production plant could induce fatal hazards and enormous loss to human lives, environments, and properties. Thus, for securing integrated design safety, core source technologies include subsea system integration that has high safety and reliability and a technique for the subsea flow assurance of subsea production plant and subsea pipeline network fluids. The evaluation of subsea flow assurance needs to be performed considering the performance of a subsea production plant, reservoir production characteristics, and the flow characteristics of multiphase fluids. A subsea production plant is installed in the deep sea, and thus is exposed to a high-pressure/ low-temperature environment. Accordingly, hydrates could be formed inside a subsea production plant or within a subsea pipeline network. These hydrates could induce serious damages by blocking the flow of subsea fluids. In this study, a simulation technology, which can visualize the system configuration of subsea production processes and can simulate stable flow of fluids, was introduced. Most existing subsea simulations have performed the analysis of dynamic behaviors for the installation of subsea facilities or the flow analysis of multiphase flow within pipes. The above studies occupy extensive research areas of the subsea field. In this study, with the goal of simulating the configuration of an entire deep sea production system compared to existing studies, a DES-based simulation technology, which can logically simulate oil production processes in the deep sea, was analyzed, and an implementation example of a simplified case was introduced.

• Current Research on Agriculture and Life Sciences
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• v.33 no.1
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• pp.7-14
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• 2015

This study identifies the complementarity or substitutability among apple production technology bundles. To identify the production technology bundle relationships, this paper focuses on the 8 most commonly used technology groups, including a high density plant, normal density plant, M9, M26, more than 70% adoption ratio of a medium-maturing variety, the adoption of a new variety, artificial pollination, and low-temperature storage. The results show that M9 has a complementary relationship with a high density plant, yet a substitute relationship with a normal density plant. Meanwhile, M26 has a complementary relationship with both a high and normal density plant. No substitute relationship is found between M9 and M26, yet a complementary relationship is identified between the adoption of artificial pollination and low-temperature storage and M9 or M26.

• Korean Journal of Medicinal Crop Science
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• v.14 no.2
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• pp.101-106
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• 2006

We established a practical method for rapid and large-scale production of Valeriana fauriei var. dasycarpa Hara roots by bioreactor culture and confirmed valerenic acids and valepotriates production. We also compared valerenic acids and valepotriates production patterns according to various media conditions. Among the media tested, B5 medium gave the maximum biomass production of 101 g fresh weight, which was a 5.03-fold multiplication rate obtained 4 weeks after inoculation of 20 g of fresh weight. The best production of total valerenic acids $(7.86\;mg/l)$ and valepotriates $(8.96\;mg/l)$ was B5 medium.

• Biomolecules & Therapeutics
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• v.25 no.4
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• pp.345-353
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• 2017

Plant expression systems have been developed to produce anti-cancer vaccines. Plants have several advantages as bioreactors for the production of subunit vaccines: they are considered safe, and may be used to produce recombinant proteins at low production cost. However, several technical issues hinder large-scale production of anti-cancer vaccines in plants. The present review covers design strategies to enhance the immunogenicity and therapeutic potency of anti-cancer vaccines, methods to increase vaccine-expressing plant biomass, and challenges facing the production of anti-cancer vaccines in plants. Specifically, the issues such as low expression levels and plant-specific glycosylation are described, along with their potential solutions.

• Korean Journal of Plant Resources
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• v.36 no.6
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• pp.588-596
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• 2023

Long-term culture of cell lines is an important issue in in vitro culture and in plant science. In this study, the regeneration ability and ex vitro acclimatization of regenerants were evaluated. The ploidy level of regenerants derived from long-term cultured cell lines was measured in Imperata cylindrica 'Rubra', Poaceae. Adventitious buds (shoots) were successfully induced from five-year-cultured calli on MS medium containing 0.1 mg/L BA or 2.0 mg/L TDZ, combined with 0.01 mg/L auxins (IAA, IBA, NAA and 2,4-D), respectively. Adventitious roots were also induced on MS medium containing 0.01 mg/L auxins (IBA, NAA and 2,4-D), respectively. Interestingly, regenerants with both red and green leaf were successfully obtained when regenerants were cultured on MS medium with 9% sucrose. Regenerants derived from long-term cultured calli were transferred to pots using an optimal acclimatization process and successfully adapted to both pot and soil conditions. Moreover, the ploidy level was measured using calli and regenerants that had been kept on MS medium containing various kinds of plant growth regulators (PGRs).

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2000.11c
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• pp.607-612
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• 2000

Microprecision agriculture for a fully controlled plant factory is proposed in this paper. Microprecision agriculture can be attained by using plant factories to realize profitable alternative agriculture. A closed, fully controlled, plant-growing factory is far better in terms of minimizing all sorts of waste. The limit and optimum design concept has to be applied to establish an economically feasible, fully controlled, plant-growing factory. To achieve this objective, microprecision technologies have to be developed. Microprecision technologies should be involved in sensing, modeling, controlling, and collecting information for the mechatronics for plant production. Basic technologies for microprecision are already available; they are SPA (speaking plant approach to environmental control), AI (artificial intelligence: expert systems, neural networks, genetic algorithms, photosynthetic algorithms etc.), bioinstrumentation, non-invasive measurement, biomechatronics, and biorobotics. A microprecision irrigation system for plug production is an example of a microprecision technology that has actually been implemented in a plug seedling production factory.

• BMB Reports
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• v.49 no.3
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• pp.149-158
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• 2016

Plants have evolved a vast chemical cornucopia to support their sessile lifestyles. Man has exploited this natural resource since Neolithic times and currently plant-derived chemicals are exploited for a myriad of applications. However, plant sources of most high-value natural products (NPs) are not domesticated and therefore their production cannot be undertaken on an agricultural scale. Further, these plant species are often slow growing, their populations limiting, the concentration of the target molecule highly variable and routinely present at extremely low concentrations. Plant cell and organ culture constitutes a sustainable, controllable and environmentally friendly tool for the industrial production of plant NPs. Further, advances in cell line selection, biotransformation, product secretion, cell permeabilisation, extraction and scale-up, among others, are driving increases in plant NP yields. However, there remain significant obstacles to the commercial synthesis of high-value chemicals from these sources. The relatively recent isolation, culturing and characterisation of cambial meristematic cells (CMCs), provides an emerging platform to circumvent many of these potential difficulties.

• Journal of the Korea Safety Management & Science
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• v.14 no.1
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• pp.117-122
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• 2012

This paper deals with the productivity increment through construction of lean production system for automobile factory in China. This lean production system has been progressed according to 5 steps. Step 1 is the work preparation. Step 2 is the process design. Step 3 is the establishment of method to count production quantity. Step 4 is the establishment of measuring method for input and output. Finally, step 5 is the construction of flexible production system able to adapt for environment change. This lean production system is expected to obtain the productivity increment by 50% for plastic plant and reduction by 50% in inventory quantity.

• Journal of the Korean Society of Industry Convergence
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• v.27 no.2_1
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• pp.269-276
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• 2024

Many countries across the world are making efforts beyond reducing CO₂ levels and declaring 'net zero,' which aims to cut greenhouse gas emissions to zero by not emitting any carbon or capturing carbon, by 2050. Hydrogen is considered a key energy source to achieve carbon neutrality goals. Korean companies are also interested in building overseas green ammonia production plants and importing hydrogen into Korea in the form of ammonia. Green hydrogen production uses renewable energy sources such as solar and wind power, but the variability of power production poses challenges in plant design. Therefore, optimization of the configuration of a green ammonia production plant using renewable energy is expected to contribute as basic information for securing the economic feasibility of green ammonia production.