• Korean Journal of Animal Reproduction
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• v.20 no.2
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• pp.179-190
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• 1996

Sexing and developing from splitted embryos which were fertilized in vitro implicate a possibility of production of the superior and sex controlled individuals. This study was carried out to investigate the production of transferable late blastocysts from in vitro fertilized embryos and to analyze sex by chromosome analysis from same embryos. In results, the ratio of cleavage and fertility of bovine follicular oocytes matured in vitro was 90% in co-cultured with granulosa cells. The competence of embryonic development from in vitro matured and fertilized bovine oocytes was 38% in co-cultured with bovine oviductal epithelial cells. To produce a lot of transferable embryos, therefore, the best conditon of culture system was co-cultured with granulosa cells for immature bovine oocytes and then co-cultured with bovine oviductal eptithelial cells for matured and fertilized oocytes. In chromosome analysis, 93% of in vitro fertilized embryos were very important aspect in chromosome preparation from bovine embryos such as duration of colcemid treatment, weakening of zona pellucida, methods of hypotonic treatment and fixation treatment.

• Asian Journal of Turfgrass Science
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• v.15 no.3
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• pp.137-146
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• 2001

Stolen and rhizome are used as planting material fur sod production in hybrid types of bermudagrass. The new technology of Leaves and Stems Dressing (LSD) uses leaves and stems collected from mowing for sod production and turf establishment. The procedure of Leaf and Stem Dressing is as follows; 1) Collection of leaves and stems of bermudagrass using rotary mower with bucket. 2) Preparation of turf bed. 3) Dressing of leaves and stems of bermudagrass by man power or spray machine. 4) first irrigation. 5) Topdressing with sand or rotary with tractor. 6) Final irrigation.

• Journal of Plant Biotechnology
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• v.6 no.2
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• pp.125-130
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• 2004

Callus and cell suspension cultures of Eurycoma longifolia Jack could be an alternative supply of 9-hydroxycanthin-6-one and 9-methoxycanthin-6-one. The callus tissues were initiated from leaves of different trees. The friable calli were used for the preparation of the cell suspension cultures of E. longifolia. The leaf explant of tree Eu-9 produced the most callus and also induced high cell biomass in the cell suspension culture, but it produced low quantity of 9-methoxycanthin- 6-one and 9-hydroxycanthin-6-one. The leaf explant from tree Eu-8 produced low quantity of callus and cell biomass, but produced the highest quantity of 9-methoxycanthin- 6-one and 9-hydroxycanthin-6-one. Optimum production of cell biomass was obtained on cell culture medium with pH 5.75 prior to autoclaving, but high alkaloids content could be induced in culture medium in acidic condition with pH 4.75 and 5.25 prior to autoclaving.

• Journal of Industrial Technology
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• v.35
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• pp.59-65
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• 2015

As the fossil fuels are depleted nowadays, development of alternative energies is absolutely required in the world. Efficient production of hydrogen by water-splitting using solar energy can be one of the methods to solve the global energy and environmental problems. But this method has a problem of low conversion efficiency. The application of graphene can be one method to help increase the conversion efficiency. For this reason, mass production of high quality graphene is required. In this study, we prepared graphene using the chemical exfoliation method. We applied the Hummer's method and Tour's method to oxidize the graphite and could get the different Graphene Oxide(GO) from different process conditions. We also tried to convert the GO to graphene by thermal reduction and could remove functional group of GO effectively. The control of oxidation conditions was quite important to obtain the high quality graphene.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.9
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• pp.1342-1351
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• 2001

Over the years, the embryo transfer industry has grown from the simple collection & transfer of embryos into an advanced field of embryo biotechnology. Currently a large demand exists for bovine oocytes and early embryos in both research and commercial settings. Bovine embryos can now be produced in-vitro. Primary oocytes collected from antral follicles of abattoir - obtained ovaries can be induced to undergo the maturation process. In-vitor maturation system, however must ensure that the resulting oocyte is capable of undergoing normal fertilization and yields a zygote competent of developing to term after embryo transfer. Sperm preparation for IVF has improved with the use of heparine. The use of co-culture system has proved beneficial in circumventing the developmental block in IVM/IVF bovine embryos.

• Journal of Microbiology and Biotechnology
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• v.3 no.4
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• pp.266-269
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• 1993

The production of free non-volatile and volatile organic acids in Kimchi during fermentations at 30, 20 and $5^{\circ}C$, were determined by gas chromatography. The order in the amount of non-volatile organic acid, soon after preparation, was malic, citric, tartaric, pyroglutamic, oxalic, lactic, succinic and ${\alpha}-ketoglutaric$ acids. The major non-volatile acids at the optimum ripening time were malic, tartaric, citric and lactic acids, and as the temperature was lowered, the amount of lactic, succinic, oxalic, pyroglutamic and fumaric acids increased, while that of malic and tartaric acids decreased. The order in the amount of volatile acids at the beginning was acetic, butyric, propionic and formic acids. Among these acids, acetic acid was significantly increased in its amount during fermentation and the Kimchi fermented at low temperature produced more acetic acid than that fermented at high temperature.

• KIEAE Journal
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• v.7 no.3
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• pp.71-79
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• 2007

The starting point of successful building production process is the feasibility study of the project. If the feasibility study is poor which is affected in direct production processes such as designing and engineering, it incurs a great unrecoverable loss to the company. The government institutionalized the feasibility study for projects that budgets over 50billion won in an effort to ensure competitiveness of the construction industry after IMF; however, the system and effort to created systematic studies or guidance preparation for private construction development projects are lacking in great deal. Therefore, the objective of this study is to establish the analysis factors of feasibility study and evaluation standard by systematic analysis in consideration of the changes of various variables and mutual relationships using system dynamics technique for office buildings among commercial buildings.

• The Korean Journal of Ceramics
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• v.4 no.4
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• pp.368-371
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• 1998

The work aims to evaluate the necessary physical properties of Abrasion Resistant Pavers designed for high volume pedestrian and road vehicle traffic and their influence on the selection of raw materials and ceramic processes. The pavers' specifications such as high strength and ware resistance demand a careful clay preparation, slow drying, slow firing and a balanced chemical and mineralogical composition. Therefore, developing abrasion Resistant Pavers in existing modern brickmaking plants, which are designed primarily for making bricks and pavers for domestic applications, has become a challenge for manufacturers and ceramic professionals. The significance of quality control and research and development in the production of these high class pavers is also emphasised in this work through the investigation of a paver that exhibits shrinkage cracking.

• Journal of the East Asian Society of Dietary Life
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• v.14 no.5
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• pp.443-448
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• 2004

In order to investigate the optimal factors for frozen dough production, the freezing and thawing condition such as temperature and time, storage period and the effect of ingredient addition were determined. A pre-fermentation of dough at 30℃ for 120 minutes was appeared to be the best for the production of frozen dough. The dough was frozen at -18℃ and then stored for 7 days. The quality of frozen dough was found to be optimal when thawed at 30℃ for 80 minutes. As ingredient of frozen dough, an addition of 3% of yeast and 4% of butter was good as well as the addition of skim milk and sugar in terms of fermentation capacity after thawing.

• BMB Reports
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• v.37 no.4
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• pp.387-393
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• 2004

The L-asparaginase (E. C. 3. 5. 1. 1) enzyme was purified to homogeneity from Pseudomonas aeruginosa 50071 cells that were grown on solid-state fermentation. Different purification steps (including ammonium sulfate fractionation followed by separation on Sephadex G-100 gel filtration and CM-Sephadex C50) were applied to the crude culture filtrate to obtain a pure enzyme preparation. The enzyme was purified 106-fold and showed a final specific activity of 1900 IU/mg with a 43% yield. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the purified enzyme revealed it was one peptide chain with $M_r$ of 160 kDa. A Lineweaver-Burk analysis showed a $K_m$ value of 0.147 mM and $V_{max}$ of 35.7 IU. The enzyme showed maximum activity at pH 9 when incubated at $37^{\circ}C$ for 30 min. The amino acid composition of the purified enzyme was also determined.