• 한국미생물·생명공학회지
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• 제24권1호
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• pp.126-131
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• 1996

HL-60 was cultivated to produce tPA (tissue-type plasminogen activator) and study the mechanism of cell death. Maximum cell density and tPA production were obtained as $5.27{\times}10^6$ cells/ml and 324ng/ml, respectively under perfusion cultivation. tPA production was enhanced to 420ng/ml in adding 160nM of phorbol ester. The cells were gradually differentiated to granulocytes rather than proliferation. By Fluorescent microscope, apoptosis was prevailed except the death phase and in high agitation speed, but necrosis was prevailed in thawed cells and during the latter periods of the cultivation. It was also proved that tPA was most produced in apoptosis. To obtain higher tPA productivity, the cells must be maintained in apoptosis, not necrosis phase when the cells were dying.

• Nutritional Sciences
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• 제9권2호
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• pp.117-123
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• 2006

G. japonicum is a perennial hem and the flowering plant has been used as a diuretic and an astringent in Japan and China. However, little information is available about the anti-inflammatory action of G. japonicum. Therefore, the objective of this study was to investigate the antiinflammatory action of fractions from G. japonicum methanol extract. Inhibition of NO production was observed when cells were cotreated with fractions of G. japonicum and lipopolysaccharide. We observed that ethyl acetate fraction of G. japonicum inhibited NO production by LPS-activated RAW 264.7 cells, and that the suppression induced by ethyl acetate fraction of G. japonicum was associated with antioxidant activity and direct NO clearance. In addition, only ethyl acetate fraction of G. japonicum inhibited stimulated $PGE_2,\;TNF-\alpha,\;IL-1\beta$ production, whereas water and methyl chloride fractions showed no such effects. The ethyl acetate fraction of G. japonicum methanol extract showed a remarkable scavenging activity on the 1,1-diphenyl-2 picrylhydrazyl radical. Based on the results, ethyl acetate fraction of G. japonicum may be useful source as natural antioxidants and antiinflammation. Therefore, the results obtained from this study provide an alternative protective mechanism of ethyl acetate fraction of G. japonicum and provide information on the potential use of ethyl acetate fraction of G. japonicum in chemoprevention or pathogenic conditions related to overproduction of NO and $PGE_2$. However, the mechanism of the inflammatory effect must be evaluated through various parameters for induction of NO production.

• 한국경제지리학회지
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• 제17권4호
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• pp.876-891
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• 2014

기존의 에스닉 인클레이브 연구는 지리학자들의 참여가 상대적으로 부진한 상황에서 에스닉 네트워크를 강조함에 따라 지리적 맥락과 산업 생산체계의 공간적 함의에 대한 설명이 별로 이루어지지 않아 이에 대한 이해가 매우 제한적이었다. 본 연구는 브라질 봉헤찌로 한인 의류산업지구를 사례로 봉헤찌로의 발전 경로와 의류산업의 생산체계 그리고 에스닉 네트워크를 함께 고찰함으로써 한인 인클레이브의 형성 메커니즘을 규명하고자 했다. 연구지역의 형성에는 에스닉 네트워크의 역할도 중요하지만, 이주자 의류 산업지역이라는 지역의 발전 경로와 의류산업의 생산체계와 관련하여 나타나는 공간적 수렴현상에도 그 원인이 있으며, 이 과정에는 비동족 이주자 집단 및 브라질 현지인들의 참여도 활발하게 이루어졌음을 알 수 있었다.

• Environmental Analysis Health and Toxicology
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• 제20권1호
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• pp.87-95
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• 2005

Cadmium is well known as a toxic metal and has insulin mimicking effects in rat adipose tissue. This study was undertaken to investigate the effect of CdCl₂ on glucose transport and its mechanism in 3T3 - L1 adipocytes. CdCl₂ exhibits respectively 2.2 and 2.8 fold increases in the 2-deoxyglucose uptake when exposed to 10 and 25 μM of CdCl₂ for 12 hr. To investigate the stimulating mechanism of glucose transport induced by CdCl₂. Wortmannin and PD98059 were used respectively as PI3K inhibitor and MAPK inhibitor, which did not affect 2-DOG uptake. This results suggest that induced 2-deoxy-(l-3H)-D-glucose (2-DOG) uptake by CdCl₂ may not be concerned with the insulin signalling pathway. Whereas nifedipine, a calcium channel blocker inhibited the 2- DOG uptake stimulated by CdCl₂. In addition, we also measured the increased production of Reactive oxygen substances (ROS) and glutathione (GSH) level in 3T3-L1 adipocytes to investigate correlation between the glucose uptake and increased production of ROS with H2DCFDA. CdCl₂ increased production of ROS. Induced 2-DOG uptake and increased production of ROS by CdCl₂ were decreased by N-acetylcystein (NAC). And L-buthionine sulfoximine (BSO) a potent inhibitor of γ-GCS, decreased of 2-DOG uptake. Also NAC and BSO changed the cellular GSH level, but GSH/GSSG ratio remained unchanged at 10, 25 μM of CdCl₂.

• Journal of Acupuncture Research
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• 제23권2호
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• pp.61-72
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• 2006

Objectives : Human chondrocytes co-treated with Buthus martensi Karsch herbal acupuncture solution(BMK-HAS) extract produced significantly less NO compared with chondrocytes stimulated with $IL-1{\beta}$ alone Methods : Activation and translocation of and NF-kB DNA binding activity were determined by Western blotting and specific enzyme-linked immunosorbent assay. Results : The inhibition of NO production correlated with the suppression of induction and expression of nuclear factor-kB (NF-kB) and activation protein-1 (AP-1)-dependent gene. BMK-HAS inhibited the activation and translocation of NF-kB to the nucleus, indicating that BMK-HAS inhibits the $IL-1{\beta}-induced$ production of NO in human chondrocytes by interfering with the activation of NF-kB through a novel mechanism. In addition, BMK-HAS reduced prostaglandin E2 (PGE2)production in mouse peritoneal macrophages stimulated with lipopolysaccharide, whereas no influence on the activity of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2) or cyclooxygenase-1 (COX-1) was observed. My data, therefore, suggest that BMK-HAS may be a therapeutically effective inhibitor of $IL-1{\beta}-induced$ inflammatory effects that are dependent on NF-kB activation in human OA chondrocytes. Conclusion : The results indicate that BMK-HAS exerts anti-inflammatory effects related to the inhibition of neutrophil functions and of NO and PGE2 production, which could be due to a decreased expression of iNOS and COX-2 through the transcription factors NF-kB and AP-1.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.8-8
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• 2019

The stringent response (SR) is characterized as a bacterial defense mechanism in response to various growth-inhibiting stresses. It is activated by accumulation of a small nucleotide regulator, (p)ppGpp, and induces global changes in bacterial transcription and translation. Recent work from our group has shown that (p)ppGpp plays a critical role in virulence and survival in Vibrio cholerae. The genes, relA and relV, are involved in the production of (p)ppGpp, while the spoT gene encodes an enzyme that hydrolyzes it in V. cholerae. A mutant strain defective in (p)ppGpp production (i.e. ${\Delta}relA{\Delta}relV{\Delta}spoT$ mutant) lost the ability to produce cholera toxin (CT) and lost their viability due to uncontrolled production of organic acids, when grown with extra glucose. In contrast, the ${\Delta}relA{\Delta}spoT$ mutant, a (p)ppGpp overproducer strain, produced enhanced level of CT and exhibited better growth in glucose supplemented media via glucose metabolic switch from organic fermentation to acetoin, a neutral fermentation end product, fermentation. These findings indicates that (p)ppGpp, in addition to its well-known role as a SR mediator, positively regulates CT production and maintenance of growth fitness in V. cholerae. This implicates SR as a promising drug target, inhibition of which may possibly downregulate V. cholerae virulence and survival fitness. Therefore, we screened a chemical library and identified a compound that induces medium acidification (termed iMAC) and thereby loss of wild type V. cholerae viability under glucose-rich conditions. Further, we present a potential mechanism by which the compound inhibits (p)ppGpp accumulation. Together, these results indicate that iMAC treatment causes V. cholerae cells to produce significantly less (p)ppGpp, an important regulator of the bacterial virulence and survival response, and further suggesting that it has a therapeutic potential to be developed as a novel antibacterial agent against cholera.

• International Journal of Computer Science & Network Security
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• 제21권11호
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• pp.81-88
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• 2021

The presented scientific research substantiates the principles of the mechanism of the investment resources involvement in order to introduce innovations at enterprises in the context of digitalization using a resource-functional approach. The importance of attracting investment resources, which contributes to the modernization of production systems, the creation of a stable economic field of development of economic entities, is justified. The expediency of application of the resource-functional approach on research of the mechanism of attraction of investment resources for introduction of innovations at the enterprises in the conditions of digitalization is proved. The investment process is presented in the form of a chain of interdependent processes which include: attraction of investment resources, investments, increase of investment value, profit. It is proved that the mechanism of attracting investment resources for the introduction of innovations in enterprises in the context of digitalization cannot be considered in isolation from the process, due to the fact that the mechanism is aimed at performing specific functions. The functions of the mechanism include management, complex, coordination, monitoring, performance and control functions. Functions of the mechanism of attraction of investment resources for introduction of innovations at the enterprises in the conditions of digitalization are caused by the purposes of attraction of investment resources for innovative development; the presence of an objective nature; relative independence and homogeneity; implementation of functions in the process of investing in innovative activities of the enterprise.

• International Journal of Computer Science & Network Security
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• 제21권11호
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• pp.111-118
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• 2021

The authors of the study highlight the conceptual foundations of the investment mechanism of innovation of enterprises in the context of strategic development. Such indicators of investment attraction for the enterprise as investment attractiveness, investment activity of the enterprise, critical mass of investments, minimum sufficiency of investments and others are singled out. It is proved that the balance of investment resources is facilitated by the action of the investment mechanism of innovation activity at the enterprise in the context of achievements of strategic development tasks. Investment processes and their intensification have an impact on the expansion of production capacity of economic entities in strategic development and on improving the efficiency of existing capacities. The investment mechanism of innovative activity at the enterprise in the context of achievement of tasks of strategic development contains system of complex actions which provides: definition of the clear purpose and tasks for the mechanism and achievement of the purposes of the enterprise; assessment of the investment potential of the enterprise; definition of tasks of innovative development of enterprises and investment resources necessary for this purpose, etc. The tasks of the investment mechanism of innovative activity at the enterprise in the context of achievements of tasks of strategic development and its economic, organizational and information components are singled out, as well as levers of influence and regulators of the investment mechanism of innovation in the enterprise.

• 대한산업공학회지
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• 제12권1호
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• pp.119-131
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• 1986

Described in this paper is a modelling methodology for mass production system simulation. The mass production system under consideration consists of various types of flow lines, special purpose production facilities, conveyor lines, palletized carts, and storage facilities. This type of production system is typical in home appliance industry, automobile industry, footwear industry, etc. where a variety of product mix are mass-produced. The modelling methodology is based on the "discrete-event formalism", and an "activity-oriented world view" is adopted to formalize the system description. A distinctive feature of the modelling methodology is that only the static structure (ie, system components) is included in the fixed model. The dynamic structure of the system is specified through a "data-driven" mechanism, which is an extension of the "experimental frame" concept. Each type of system components (ie, flow line, conveyors, carts, etc.) is formally modeled by using Activity Cycle Diagrams. The issue of "model structuring" is also addressed. The modeling methodology has been successfully applied in a real simulation study of a mass production system.

• 한국대기환경학회:학술대회논문집
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• 한국대기환경학회 2007년도 추계학술대회 논문집
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• pp.535-537
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• 2007