• Asian-Australasian Journal of Animal Sciences
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• v.16 no.6
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• pp.910-927
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• 2003

Transgenesis is a very powerful tool not only to help understanding the basics of life science but also to improve the efficiency of animal production. Since the first transgenic mouse was born in 1980, rapid development and wide application of this technique have been made in laboratory animals as well as in domestic animals. Although pronuclear injection is the most widely used method and nuclear transfer using somatic cells broadens the choice of making transgenic domestic animals, the demand for precise manipulation of the genome leads to the utilization of gene targeting. To make this technique possible, a pluripotent embryonic cell line such as embryonic stem (ES) cell is required to carry genetic mutation to further generations. However, ES cell, well established in mice, is not available in domestic animals even though many attempt to establish the cell line. An alternate source of pluripotent cells is embryonic germ (EG) cells derived from primordial germ cells (PGCs). To make gene targeting feasible in this cell line, a better culture system would help to minimize the unnecessary loss of cells in vitro. In this review, general methods to produce transgenic domestic animals will be mentioned. Also, it will focus on germ cell engineering and methods to improve the establishment of pluripotent embryonic cell lines in domestic animals.

• Korean Journal of Computational Design and Engineering
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• v.19 no.2
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• pp.148-156
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• 2014

Generally, over 95% of manufacturing cost is determined in the design and manufacturing preparation step, especially a great part of productivity is determined in the manufacturing preparation step. In order to improve the manufacturing competitiveness, we have to verify the problems that can be occurred in the production step and remove the unnecessary factors in the manufacturing preparation step. Thus, manufacturing industries are adopting digital manufacturing system based on modeling & simulation. In this paper, we introduce e-FEED system (electronic based Front End Engineering and Design) that is the integrated design and analysis system for optimized manufacturing line development based on simulation automation and explain the work process (Design, Analysis and Optimization) about manufacturing line development using e-FEED system. Also, the effect is described through the real implementation cases.

• Proceedings of the KIEE Conference
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• 1999.07b
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• pp.668-670
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• 1999

We have developed a automatic surface inspection system for cold Rolled strips in steel making process for several years. We have experienced the various kinds of surface inspection systems, including linear CCD camera type and the laser type inspection system which was installed in cold rolled strips production lines. But, we did not satisfied with these inspection systems owing to insufficient detection and classification rate, real time processing performance and limited line speed of real production lines. In order to increase detection and computing power, we have used the Dark Field illumination with Infra_Red LED, Bright Field illumination with Xenon Lamp, Parallel Computing Processor with Area typed CCD camera and full software based image processing technique for the ease up_grading and maintenance. In this paper, we introduced the automatic inspection system and real time image processing technique using the Object Detection, Defect Detection, Classification algorithms. As a result of experiment, under the situation of the high speed processed line(max 1000 meter per minute) defect detection is above 90% for all occurred defects in real line, defect name classification rate is about 80% for most frequently occurred 8 defect, and defect grade classification rate is 84% for name classified defect.

• Journal of the Korean Society of Clothing and Textiles
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• v.20 no.1
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• pp.54-65
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• 1996

The purposes of this study were 1) to present the optimum slacks pattern for young men, 2) to develope a methodology to draft basic slacks pattern using AutoCAD The total crotch legth and the shape of the crutch line were determined by anthropometric data analysis. The total crotch length was calculated with the waist girth, the hip girth and the crotch length measurements. The anthropometric data utilized for this procedure was National Anthropometric Survey of Korea, 1992. And multidimensional anthropometric measurements were carried out for 6 male college students between the age of 18 to 24. The subjects were measured with the Martin's anthropometer and the sliding gauge. Mean, standard deviation and t-test were performed for statistical analysis of the data. The automatic drafting method was programmed by AutoLISP in AutoCAD. The automatic drafting was based on the Muller's slacks pattern drafting method, the measurements of slacks construction components and the curve of crotch line. The crotch line was drafted using of the arc function in AutoCAD. The total crotch length was calcuated using the multiple regression equation. The experimental pattern developed to accomodate individual body wleasurements expected to produce customized apparel production in QRS(Quick Response System) production system.

• Journal of Microbiology and Biotechnology
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• v.16 no.10
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• pp.1485-1490
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• 2006

In this study, comparative replicational properties of Hyphantria cunea nucleopolyhedrovirus (HycuNPV) in Lymantria dispar (IPLB-LdElta) and Spodoptera frugiperda (IPLB-Sf21) cell lines were investigated. Our microscopic observations showed that cytopathic effects (CPEs) in LdElta cells appeared 12 h later than those in Sf21 cells. Whereas polyhedral inclusion bodies (PIBs) formed at 48 h postinfection (p.i.) in LdElta cells, it formed at 36 h p.i. in Sf21 cells. Extracellular virus production determined according to the 50% tissue culture infective dose ($TCID_{50}$) method in LdElta cells started about 12 h later when compared with Sf21 cells. Titers of extracellular virus in LdElta and Sf21 cells were calculated as $1.77{\times}10^9$ plaque forming units (PFU)/ml and $5.6{\times}10^9PFU/ml$, respectively, at 72 h p.i. We also showed that viral DNA replication began at 12 h p.i. in both cell lines. Viral protein synthesis was determined by SDS-polyacrylamide gel electrophoresis (PAGE) and polyhedrin synthesis was observed at 12 h p.i. in both cell lines. The results indicate that while the synthesis of macromolecules is 12 h later and production of extracellular virus is almost 3-fold lower in LdElta cells compared with those in Sf21 cells, the LdElta cell line is still a productive cell line for infection of HycuNPV.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.9
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• pp.1276-1284
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• 2014

In southeastern regions of the US, herbage systems are primarily based on grazing or hay feeding with low nutritive value warm-season perennial grasses. Nutritious herbage such as annual ryegrass (Lolium multiflorum Lam.) may be more suitable for preserving as baleage for winter feeding even with more intensive production inputs. Emerging in-line wrapped baleage storage systems featuring rapid wrapping and low polyethylene film requirements need to be tested for consistency of storing nutritive value of a range of annual ryegrass herbage. A ryegrass storage trial was conducted with 24-h wilted 'Marshall' annual ryegrass harvested at booting, heading and anthesis stages using three replicated in-line wrapped tubes containing ten round bales per tube. After a six-month storage period, nutritive value changes and fermentation end products differed significantly by harvest stage but not by bale location. Although wilted annual ryegrass exhibited a restricted fermentation across harvest stages characterized by high pH and low fermentation end product concentrations, butyric acid concentrations were less than 1 g/kg dry matter, and lactic acid was the major organic acid in the bales. Mold coverage and bale aroma did not differ substantially with harvest stage or bale location. Booting and heading stage-harvested ryegrass baleage were superior in nutritive value to anthesis stage-harvested herbage. Based on the investigated nutritive value and fermentation characteristics, individual bale location within in-line tubes did not significantly affect preservation quality of ryegrass round bale silages.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.1
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• pp.59-63
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• 1999

Yield and growth characteristics were compared for five rice cultivars; a new Tongil-type, so called "super-rice", Dasanbyeo, an old Tongil-type Milyang 23, two japonicas Dongjinbyeo and Ilpumbyeo, and a new plant type (NPT)line IR65600-27-1-2. The objective of this stusy was to clarify the high yielding capacity of Dasanbyeo in terms of growth characteristics. The average grain yield (9 t/ha) of Dasanbyeo was higher than that of Milyang 23 by ca. 9% that of japonicas by 20 to 30%, and that of NPT line by ca. 100%. The higher grain yield of Dasanbyeo was attributable not only to the greater dry matter production but also to the higher harvest index (HI). Dasanbyeo showed the greatest dry matter at harvest owing not only to the rapid leaf expan-sion at early growth stage and the resulting high LAI through the entire growth stage but also to the high NAR despite the high LAI. The rapid leaf expansion of Dasanbyeo at early growth stage seemed to be related in part to the profuse tillering capacity. HI was 0.53 in Dasanbyeo, 0,51 in Milyang 23, 0.41 in japonicas, and 0.35 in NPT line. Dasanbyeo was indebted for its higher HI to the relatively high grain filling ratio in spite of a much greater sink size than the other cultivars. Dasan had a greater source to sink ratio during grain ripening as measured by LAD/spikelet and dry matter production/spikelet which showed positove correlations with the grain ripening ratio. New plant type (NPT) line showed the lowest grain yield owing to the small sink size and the low grain filling ratio which seemed to have resulted from the abundant occurrence of weak-strength spikelets. The weak sink strength, in turn, seemed to have suppressed photosynthesis during the grain ripening stage.

• Textile Coloration and Finishing
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• v.22 no.3
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• pp.282-291
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• 2010

Digital textile printing(DTP) is becoming more important because the production trend of textile printing goods is adapting to small-lot multiple items. Recently enhanced use of DTP is closely connected with production of high value-added products in fashion industry, which is also appropriate for quick response system(QRS). Quality of DTP depends on pre-treatment, after-treatment, ink, media, printer, etc. One of these parameters, Selection of good media is very important to obtain high quality of DTP products. Especially, the effects of media on printing quality of DTP according to yarn twist and structure of knitting fabric were examined in this study. Two types of yarn twist of 830 t.p.m and 1630 t.p.m for cotton knit were used and five types of media structures were knitted with single circular knitting machine. First, MIU, MMD, SMD's values are closely related with surface roughness of sample as well as printing quality. The hard twist samples were higher values than normal twist samples in the same media structure. In case of SMD, the values increased from plain to corduroy types. Second, aspect of line sharpness, line area, and line width values of hard twist samples decreased from plain to corduroy than those of normal twist samples. Third, line deviation values of hard twist samples, blurriness of line, also decreased than those of normal twist samples.

• Korean Journal of Breeding Science
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• v.42 no.3
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• pp.298-301
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• 2010

A new intermediate parent 'Wonye 30002' (Allium cepa L.) was developed by Bioenergy Crop Research Center, NICS in 2009. As a male sterile line, 'Wonye 30002' can be used to hybrid seed production by crossing with pollen parent. The first cross was conducted between male sterile plants of 402AC203 and M1 in 2002. The male sterile line 'Wonye 30002' has circular bulb and bulb weight is 283 g. As early maturing type, lodging date is May 6. Plant height and pseudostem diameter are 43 cm and 15.5 mm, respectively. In seed harvesting characteristics, number of flower stalks and the length are 43 cm and 110 cm, respectively. The flowering date of 'Wonye 30002' is around May 24 and is completely male sterile. 'Wonye 30002' is a promising male sterile line for hybrid bulb onion seed production.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.43 no.4
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• pp.23-32
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• 2020

Many small and medium-sized manufacturing companies process various product types to respond different customer orders in a single production line. To improve their productivity, they often apply batch processing while considering various product types, constraints on batch sizes and setups, and due date of each order. This study introduces a batch scheduling heuristic for a production line with multiple product types and different due dates of each order. As the process times vary due to the different batch sizes and product types, a recursive equation is developed based on a flow line model to obtain the upper bound on the completion times with less computational complexity than full computation. The batch scheduling algorithm combines and schedules the orders with same product types into a batch to improve productivity, but within the constraints to match the due dates of the orders. The algorithm incorporates simple and intuitive principles for the purpose of being applied to small and medium companies. To test the algorithm, two case studies are introduced; a high pressure coolant (HPC) manufacturing line and a press process at a plate-type heat exchanger manufacturer. From the case studies, the developed algorithm provides significant improvements in setup frequency and thus convenience of workers and productivity, without violating due dates of each order.