• Korean Journal of Poultry Science
• /
• v.12 no.1
• /
• pp.1-6
• /
• 1985

To secure a higher percentage of fertility in hatching eggs and a higher percentage of hatchability of the fertile eggs should be of direct benefit to poultry breeder ana hatchery operators. This study was carried out to estimate the relationships of egg production and fertility in broiler breeder. The data analysed were the record of pullets (5 strains) of broiler breeder raised at Poultry Breeding Farm, Seoul National Univ. from 1982 to 1983. Since the purpose of the study was only to find out the relationships between egg Production and fertility, many other factors thought to be included in fertility were fixed as much as possible. The results were summarized as follows. 1. Mean figure of egg production rate and fertility from 30 weeks age to 60 Weeks of age to 60 weeks of age were 60.5-65.2 percent and 82.7-87.9 percent. 2. Correlation coefficients between egg Production rate and fertility were high and positive, except line K, and the coefficients have highly significant differences. 3. Regression coefficients of fertility on egg Production rate were 0.54-0.97, except line K. 4. Maximum fertility age estimated from the polynomial regression equation were 44-47 weeks. The results obtained in this study suggested that the selection of high performance strain in egg Production should improve the fertility.

• Animal cells and systems
• /
• v.16 no.4
• /
• pp.274-279
• /
• 2012

Thymoquinone (TQ), a drug extracted from the black seeds of Nigella sativa, has been shown to exhibit anti-inflammatory, anti-oxidant, and anti-neoplastic effects in numerous cancer cells. The effects of TQ on cyclooxygenase-2 (COX-2) expression and prostaglandin $E_2$ ($PGE_2$) production in MDA-MB-231, however, remain poorly understood. Western blot analysis and immunofluorescence staining were performed to study the expression levels of inflammation regulatory proteins in MDA-MB-231. $PGE_2$ assay was conducted to explore the TQ-induced production of $PGE_2$. In this study, we investigated the effects of TQ on COX-2 expression and $PGE_2$ production in MDA-MB-231. TQ significantly induced COX-2 expression and increased $PGE_2$ production in a dose-dependent manner, as determined by a Western blot analysis and $PGE_2$ assay. Furthermore, the activation of Akt and p38 kinase, respectively, was up-regulated in TQ treated cells. Inhibition of p38 kinase with SB203580 and PI3kinase (PI3K) with LY294002 abolished TQ-caused COX-2 expression and decreased $PGE_2$ production. These results collectively demonstrate that TQ effectively modulates COX-2 expression and $PGE_2$ production via PI3K and p38 kinase pathways in the human breast cancer cell line MDA-MB-231.

• Journal of Microbiology and Biotechnology
• /
• v.23 no.8
• /
• pp.1116-1122
• /
• 2013

Cell line development is the most critical and also the most time-consuming step in the production of recombinant therapeutic proteins. In this regard, a variety of vector and cell engineering strategies have been developed for generating high-producing mammalian cells; however, the cell line engineering approach seems to show various results on different recombinant protein producer cells. In order to improve the secretory capacity of a recombinant tissue plasminogen activator (t-PA)-producing Chinese hamster ovary (CHO) cell line, we developed cell line engineering approaches based on the ceramide transfer protein (CERT) and X-box binding protein 1 (XBP1) genes. For this purpose, CERT S132A, a mutant form of CERT that is resistant to phosphorylation, and XBP1s were overexpressed in a recombinant t-PA-producing CHO cell line. Overexpression of CERT S132A increased the specific productivity of t-PA-producing CHO cells up to 35%. In contrast, the heterologous expression of XBP1s did not affect the t-PA expression rate. Our results suggest that CERT-S132A-based secretion engineering could be an effective strategy for enhancing recombinant t-PA production in CHO cells.

• Journal of Korea Society of Industrial Information Systems
• /
• v.8 no.4
• /
• pp.55-63
• /
• 2003

The game & multimedia industry is a high-valued industry comparing to any industry. Also, it has been shown the possibility as considerably bright future industry due to a development of the Internet, an explosive growth of on-line game market, and a union with virtual reality technology Since an on-line game having a clear profit model is expanding to PC and other platform, it brings to us a large change throughout the whole game industry. In order to develop a game, we need a number of elements. A game character among them delivers to a user the overall characteristics of a game as a visual representation and makes us access to a game directly and sensuously. A game character of on-line game is considerable in an operation in the volume of initial sales from a viewpoint of marketing. In addition, it affects to secure users and be immersed in a game. In this paper, we propose a production skill of an efficient 3D game character for 3D on-line game.

• IE interfaces
• /
• v.10 no.2
• /
• pp.115-125
• /
• 1997

The main purpose of this study is to establish standard times of several manufacturing processes in a small-sized manufacturing company located in Taegu. This company produces two types of products; single parts and part assemblies of bicycles. Due to lack of the standards on daily or hourly production volumes, the production manager could not assign workers daily production volume at the start of production, and could not exercise tight supervision. The standard times established in this study were much less than the average production times found in the daily production report. Based on these standard times, we calculated the number of machines that a worker can operate properly without bringing overload on him, and established a line balanced production system in each manufacturing process to minimize work-in-process inventory.

• Proceedings of the Safety Management and Science Conference
• /
• 2003.11a
• /
• pp.203-206
• /
• 2003

Each method for economic evaluation has its own characteristics. Therefore adoption of each of them in evaluation production investment project results in many problems. Hence combination & modification of them are required to perform more accurate evaluation about investment project. This paper discuss evaluation method of investment projects expansion and replacement investment on each line or individual in the production. Generally investment evaluation method has add to a few method by Subsidiary means with use a especial method. And then in this paper, a Taguchi Techniques is presented, which may be effective to the facilities appraisal or improvement. We propose a decision model to incorporates the values assigned by a group of experts on different factors in production. Using this model, SN ratio of taguchi method for each of subjective factors as well as values of weights are used in this comprehensive method for reducing production rate in production management.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.196-196
• /
• 2017

Oilseed crop Camelina (Camelina sativa L.) is a suitable for biodiesel production that has high adaptability under low-nutrient condition like marginal land and requires low-input cost for cultivation. Enhanced abiotic stress tolerance of Camelina is very important for oil production under the wide range of different climate. CsRCI2s (Rare Cold Inducible 2) are related proteins in various abiotic stresses that predicted to localized at plasma membrane (PM) and endoplasmic reticulum (ER). These proteins are consist of eight-family that can be divided into tail (CsRCI2D/E/F/G) and no-tail (CsRCI2A/B/E/H) type of C-terminal. However, it is still less understood the function of C-terminal tail. In this study, CsRCI2D/H genes were cloned through gateway cloning system that used pCB302-3 as destination vector. And we used agrobacterium-mediated transformation system for generation of overexpression (OX) transformants. Overexpression of target gene was confirmed using RT-PCR and segregation ratio on selection media. We analyzed physiological response in media and soil under abiotic stresses using CsRCI2D and CsRCI2H overexpression plant. To compare abiotic stresses tolerance, wild type and CsRCI2D/H OX line seeds were sown on agar plate treated with various NaCl and mannitol concentration for 7 days. In the test of growth rate under abiotic stress on media, CsRCI2H OX line showed similar to NaCl and mannitol stress. In the other hand, CsRCI2D OX line showed to be improved stress tolerance that especially increased in 200mM NaCl but was similar on mannitol media. In greenhouse, WT and CsRCI2D/H OX lines for physiological analysis and productivity under abiotic stresses were treated 100, 150, 200mM NaCl. Then it was measured various parameters such as leaf width and length, plant height, total seed weight, flower number, seed number. CsRCI2H OX line in greenhouse did not show any changes in physiological parameters but CsRCI2D OX line was improved both physiological response and productivity under NaCl stress. Among physiological parameters of CsRCI2D OX line under NaCl stress, leaf length and width were observed shorter than WT but it were slightly longer than WT in 200mM NaCl stress. Furthermore, total seed weight of CsRCI2D OX line under stress displayed to decrease than WT in normal condition, but it was gradually raised with increasing NaCl stress then more than WT relatively. These results suggested CsRCI2D might be contribute to improve abiotic stress tolerance. However, function of CsRCI2H is need to more detail study. In conclusion, overexpression of CsRCI2s family can generate various environmental stress tolerance plant and may improve crop productivity for bio-energy production.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.55 no.2
• /
• pp.152-161
• /
• 2019

This research carried out a study on the job characteristics of the skipper of the coastal composite fishing vessels in order to find a way to prevent the ship collision caused by the highest human error among the marine casualty of fishing boats. Video observation was used as the research method in which six CCD cameras were installed on the vessel to collect image data and data extracted from the image were analyzed to derive the results of the functional activity of skipper according to the fishing operation process of experimental fishing vessel. The results are as follows. The working process of the experimental fishing vessel consisted of navigation for fishing ground, setting line, waiting for hauling line, hauling line and navigation to homeport. In these processes, the skipper was performing watchkeeping in the wheelhouse in which he carried out a single task, a dual task that performed two tasks simultaneously, and a triple task that performed two or more tasks simultaneously. In addition, one of the risk factors causing the collision was a no watchkeeping in the wheelhouse for navigating for fishing ground, waiting for hauling line, and hauling line at 25.4%, 64.6% and 0.3%, respectively among the marine casualty while drowsiness caused 1.2% of the marine casualty in navigating for fishing ground. Concurrent tasks that simultaneously perform two or more tasks that can overlook any other important duties while carrying out watchkeeping in the wheelhouse include 51.3% of navigation for fishing ground, 81.9% of setting line, 19.0% of waiting for hauling line, 87.9% of hauling, and 88.7% of navigation to homeport. The above concurrent tasks yielded an average of 66.1%. Experimental fishing vessels are required to focus on ship handling operations related to fishery operations, and the skipper is assigned more activities and attention to fishery related tasks. Therefore, it is considered desirable to build a collision prevention system that is appropriate to the characteristics of the skipper's work, escaping from transferring the responsibility of ship collision to the skipper completely.

• Journal of Institute of Control, Robotics and Systems
• /
• v.19 no.3
• /
• pp.262-267
• /
• 2013

This paper describes a vision based automation case study for the tire tread extruder line. To accurately measure the thread widths, two cameras with laser line illumination have been installed near the takeaway conveyer. The overall tread extruder line is then automated by controlling the speeds of take away conveyor and screw motor such that a difference between measured widths and the targeted data is minimized. By doing this, the conventional tread extruder line has been replaced by the developed automated computer system and with only one operator, increasing the production efficiency and reducing safety accidents.

• 제어로봇시스템학회:학술대회논문집
• /
• 1997.10a
• /
• pp.1840-1843
• /
• 1997

The task environment of a robot is changing rapidly and task itself becomes complicated due to current industrial trends of multi-product and small lot size production. A convenient user-interfaced off-line programming(OLP) system is being developed in order to overcome the difficulty in teaching a robot task. Using the OLP system, operators can easily teach robot tasks off-line and verify feasibility of the task through simulation of a robot prior to the on-line execution. However, some task errors are inevitable by kinematic differences between the robot model in OLP and the actual robot. Three calibration methods using image information are proposed to compensate the kinematic differences. These methods compose of a relative position vector method, three point compensation method, and base line compensation method. To compensate a kinematic differences the vision system with one monochrome camera is used in the calibration experiment.