• Journal of Microbiology and Biotechnology
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• v.15 no.2
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• pp.296-301
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• 2005

The effect of aqueous methanol on the catalytic properties of porcine pancreatic lipase has been investigated. The k$_{CAT}$, values for the hydrolysis of N$^{alpha}$-benzyloxycarbonyl-L­lysine p-nitrophenyl ester at 0$^{circ}$C increased in a linear manner with increasing methanol concentration. However, the K$_{M}$ values were not influenced at methanol concentrations lower than $30\%$ and then began to increase at higher concentrations in an exponential fashion. Based on product analysis, the increase in k$_{CAT}$, with increasing methanol concentration can be accounted for by nucleophilic competition of methanol for the acyl enzyme intermediate, indicating that the rate-limiting step of the porcine pancreatic lipase-catalyzed reaction is deacylation under current experimental conditions. The exponential increase in K$_{M}$ at methanol concentrations higher than $30\%$ is attributed to the hydrophobic partitioning effect on substrate binding. There was no loss of lipase activity over a 4 h period in $60\%$ methanol concentration at pH$^{circ}$ 5.5 and 0$^{circ}$C. By monitoring the intrinsic fluorescence and absorbance, no evidence for structural changes by methanol was observed.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2016.05a
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• pp.207-208
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• 2016

The most important things for the production of recycled aggregates are saving energy, suppressing the generation of by-product fine particles and sustaining the performance of concrete. As solutions, this study proposes this technology of improving the performance of recycled aggregates through forced carbonation.1) It is to stimulate and carbonate the bond paste part that causes the deterioration of recycled aggregates. Particularly, the purpose of this technology is to fill and chemically stabilize pores inside the bond paste, further improving the quality of recycled aggregates with a decreased absorption rate and an enhanced aggregate strength. Ultimately, it is possible to obtain a carbonation model, depending on the paste ratio and particle-size distribution of recycled aggregates. Moreover, by calculating the optimum carbonation period through the verification of this carbonation model, it is possible to examine how much the strength is improved by the reformation of recycled aggregated.

• The Journal of the Korea institute of electronic communication sciences
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• v.13 no.1
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• pp.53-60
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• 2018

The ICT concept has been introduced to realize a highly productive smart factory and respond to the demand for small quantity and mass production between textile processes. ICT convergence monitoring system that can produce high productivity textile products by improving product development period, cost, quality and delivery time through ICT based production and optimization of manufacturing process is needed. In this paper, we propose and implement a system design that senses the amount of remaining sewing material using a non-contact sensor that can be mounted on a sewing machine and displays it on a display using IOT-based LATTE-PANDA board.

• Proceedings of the Safety Management and Science Conference
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• 2013.11a
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• pp.39-48
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• 2013

Recently, the using of prefabricated temporary equipments is increasing in the industrial field because it can reduce the installation and dismantling period. The various types of prefabricated temporary equipments are manufacturing with various materials in domestic and overseas countries. However, those equipments should win the safety certificate according to the "Governmental Notification about Safety Certification" which regulate the member-based safety certification. It tends to cause over performance of temporary equipments because it couldn't consider structural benefit of the prefabricated products. As the result of this study, it is concluded that the establishment of new safety certification standard for the prefabricated temporary equipments is resonable and the movable scaffold is appropriate for those prefabricated temporary equipment. The movable scaffolds are using as single structure and cannot be expands horizontally. Other types of temporary equipments are using as complex structure which can freely expand horizontally according to the main structure. From the results of the study, the standard of vertical compression performance of prefabricated movable scaffolds should be more than double the performance of single main frame. Other test items of safety certification standard should be fixed when the Notification is amended. The prefabricated temporary equipments which are adopted for the safety standard on assembled structure should be used, moved and kept in assembled state and not separated discretionally. The establishment of new standard for the assembled structure based on this study leads to the development of various types of temporary equipments using structural efficiency of prefabricated structure.

• YAKHAK HOEJI
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• v.34 no.3
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• pp.161-165
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• 1990

An automated, simple, and reliable method was developed for determining in vitro drug release rate from transdermal delivery dosage forms. The patch is held in position in the heating block by sandwiching it between the middle plate and the bottom plate of diffusion cell. The dissolution profile of the commercially available transdermal scopolamine patch was determined over a 72-h period, and the results were compared with those obtained with other methods; paddle-over-disk method, reciprocating method, and diffusion cell method. It was demonstrated that the flow-through method is equivalent in terms of release rate profile and accumulated released drug amount over the lifetime of the dosage form tested. Also this method is simple, reliable and reproducible. Therefore, this technique can be used in a quality control for assuring product uniformity.

• Journal of the Korean Society for information Management
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• v.32 no.4
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• pp.289-305
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• 2015

This study aims to investigate information seeking behavior of Popshoes users. Transaction logs of Popshoes, a major Korean shopping search engine, were analyzed. These transaction logs were collected over 3 months period, from January 1 to March 31, 2015. The results of this study show that Popshoes users behave in a simple and passive way. In the total sessions, more users chose to browse a directory than typing and submitting a query. However, queries played a more crucial role in important decision makings such as search results clicks and product purchases than directory browsing. The results of this study can be implemented to the effective development of shopping search engines.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.5 no.4
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• pp.7-12
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• 2006

Galvanized steel pipe, copper pipe and stainless steel pipe, which is being used in waterworks piping materials. In case of galvanized steel pipe, the precipitation of a product is being generated due to the pollution of the tap water, a white water phenomenon, and various corrosion reaction because a zinc ion is melted by tap water. And in case of a cupper pipe, many problems which is harm in sanitation appeared because of a inflow of harmfulness substance by a frequent accident of a water leakage. So, to prevent these problems, it is substituted for stainless steel pipe. However, those problems is still occurring because of badness of welding, a problem of a water leakage in connection part, and a increment of construction expenses. Therefore, this research has examined the laying period according to each piping thickness and a corrosion shape according to each laying depth after laying in various soils(sandy loam, loamy, clay loam, clay) using galvanized steel pipe, copper pipe, and stainless steel pipe. That is, we has studied the data which is necessary for a rational method of preserving the quality of water by examining the corrosion properties of piping materials in the soil environment which waterworks piping materials is being used.

• Proceedings of the Korea Concrete Institute Conference
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• 2004.11a
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• pp.537-540
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• 2004

Recently, for industrial development period, concrete structures in domestics have been increased. They were deteriorated by attack of carbonation, freeze-thaw and corrosion etc. In hence they were demolished and reconstructed, resulted in waste concrete particles. In this paper, waste concrete particles (WCP) by product from different crushing and selecting process were used in soil cement-based pavement in the various recycling. For using WCP in soil cement-based pavement, the Qualities, physical and chemical properties, of WCP should be researched. In the first step, the specified compressive strength of mortar for two types of clay sand soil and clay soil respectively was experimented to be 15 Mpa and then optimum mixing ratio of chemical solidification agent were decided in the range of $1.5\~3.0\%$ in the replacement with cement weight content. In the second step, based on the prior experimental results, recycling possibility of WCP in soil cement-based pavement was studied. In the result of experiment the mixing ratio of WCP were 5, 10, 15 and $20\%$ in the replacement with soil weight and the compressive strength of mortar was somewhat decreased according to the increase of the mixing ratio of WCP.

• Journal of the Korean Society of Safety
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• v.28 no.3
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• pp.33-38
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• 2013

Many occupational workers or professionals have to walk on the various floors for a long period of time. The objective of this study was to develop the safety shoes with increased traction through the material selection. In order to fulfill our objective, first, two kinds of filler were selected to compare the wear mechanism at outsole surface. The developed rubber materials were tested with two kinds of portable slip meters. The sample safety shoes with developed rubber materials were also tested with subject in the laboratory. During walking, the safety shoes were naturally abraded with counter surface. The coefficient of friction(COF) was gradually decreased with number of steps to 30,000, while the COF was abruptly increased from 30,000 to 40,000. The experimental results showed that COF tested with silica rubber was at least 10% higher than that with carbon black rubber in wet or detergent condition. It has been well recognized that filler properties play a important role in wet traction in the tire industry. However it has been unclear that filler properties would be decisive factor in safety shoes. Our study shows that silica exhibits a higher slip resistance than carbon black without reference to wear states in wet or detergent condition. So, this results will provide guides for outsole compounders to develop new products and improve product performance.

• Applied Microscopy
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• v.28 no.4
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• pp.539-549
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• 1998

The silk glands of the spiders are of several types. Among the several types of spider's silk glands, the principal fibers used in constructing the eggcase are products of the tubuliform glands (TBG), which are present only in females. Development of these glands parallels maturations of the ovaries and fat bodies. In order to understand the mechanism of eggcase-silk production, this paper has examined the fine structural changes of the TBG during the period of egg maturation in the garden spider, Argiope aurentia. Between the two kinds of secretory granules observed in the glandular epithelium of the mature TBG, the electron-dense granules which have paracrystalline structure are revealed to be the precursors of the eggcase silk fibers. During the production of eggcase, rapid release of the secretory product occurs at apical surface by the mechanism of apocrine secretion. Moreover, secondary lysosomes appear due to the rapid disorganization of cellular components during the eggcase formation. Examinations of formed fibers indicate a multicomponent internal structure, and electron micrographs reveal each fiber contains numerous electron lucent fibrils embedded in an amorphous electron dense matrix. The secretory precursors are produced as separated vesicles via well-oriented rER, and no Golgi complex has been found in the glandular epithelial cells.