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Large scale enzymatic production of chitooligosaccharides and their biological activities (키토산올리고당의 효소적 대량생산 및 생리활성)

  • Kim, Se-Kwon;Shin, Kyung-Hoon
    • Food Science and Industry
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    • v.53 no.1
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    • pp.2-32
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    • 2020
  • In recent years, significant importance has been given to chitooligosaccharides (COS) due to its potent notable biological applications. COS can be derived from chitosan which is commonly produced by partially hydrolyzed products from crustacean shells. In order to produce COS, there are several approaches including chemical and enzymatic methods which are the two most common choices. In this regard, several new methods were intended to be promoted which use the enzymatic hydrolysis with a lower cost and desired properties. Hence, the dual reactor system has gained more attention than other newly developed technologies. Enzymatic hydrolysis derived COS possesses important biological activities such as anticancer, antioxidant, anti-hypersentive, anti-dementia (Altzheimer's disease), anti-diabeties, anti-allergy, anti-inflammatory, etc. Results strongly suggest that properties of COS can be potential materials for nutraceutical, pharmaceutical, and cosmeceutical product development.

Southeast Asian Hindu Art from the 6th to the 7th Centuries (6-7세기의 동남아 힌두 미술 - 인도 힌두미술의 전파와 초기의 변용 -)

  • Kang, Heejung
    • The Southeast Asian review
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    • v.20 no.3
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    • pp.263-297
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    • 2010
  • The relics of the Southeast Asian civilizations in the first phase are found with the relics from India, China, and even further West of Persia and Rome. These relics are the historic marks of the ancient interactions of various continents, mainly through the maritime trade. The traces of the indic culture, which appears in the historic age, are represented in the textual records and arts, regarded as the essence of the India itself. The ancient Hindu arts found in various locations of Southeast Asia were thought to be transplanted directly from India. However, Neither did the Gupta Hindu Art of India form the mainstream of the Gupta Art, nor did it play an influential role in the adjacent areas. The Indian culture was transmitted to Southeast Asia rather intermittently than consistently. If we thoroughly compare the early Hindu art of India and that of Southeast Asia, we can find that the latter was influenced by the former, but still sustained Southeast Asian originality. The reason that the earliest Southeast Asian Hindu art is discovered mostly in continental Southeast Asia is resulted from the fact that the earliest networks between India and the region were constructed in this region. Among the images of Hindu gods produced before the 7th century are Shiva, Vishnu, Harihara, and Skanda(the son of Shiva), and Ganesha(the god of wealth). The earliest example of Vishnu was sculpted according to the Kushan style. After that, most of the sculptures came to have robust figures and graceful proportions. There are a small number of images of Ganesha and Skanda. These images strictly follow the iconography of the Indian sculpture. This shows that Southeast Asians chose their own Hindu gods from the Hindu pantheon selectively and devoted their faiths to them. Their basic iconography obediently followed the Indian model, but they tried to transform parts of the images within the Southeast Asian contexts. However, it is very difficult to understand the process of the development of the Hindu faith and its contents in the ancient Southeast Asia. It is because there are very few undamaged Hindu temples left in Southeast Asia. It is also difficult to make sure that the Hindu religion of India, which was based on the complex rituals and the caste system, was transplanted to Southeast Asia, because there were no such strong basis of social structure and religion in the region. "Indianization" is an organized expansion of the Indian culture based on the sense of belonging to an Indian context. This can be defined through the process of transmission and progress of the Hindu or Buddhist religions, legends about purana, and the influx of various epic expression and its development. Such conditions are represented through the Sanskrit language and the art. It is the element of the Indian culture to fabricate an image of god as a devotional object. However, if we look into details of the iconography, style, and religious culture, these can be understood as a "selective reception of foreign religious culture." There were no sophisticated social structure yet to support the Indian culture to continue in Southeast Asia around the 7th century. Whether this phenomena was an "Indianization" or the "influx of elements of Indian culture," it was closely related to the matter of 'localization.' The regional character of each local region in Southeast Asia is partially shown after the 8th century. However it is not clear whether this culture was settled in each region as its dominant culture. The localization of the Indian culture in Southeast Asia which acted as a network connecting ports or cities was a part of the process of localization of Indian culture in pan-Southeast Asian region, and the process of the building of the basis for establishing an identity for each Southeast Asian region.

The Aspect to Receive Pre-Chin Study of One Hundred Schools in Cho Sun Dynasty-Centered on the criticism and understanding of Hsün Tzu (조선조에서의 선진(先秦) 제자학(諸子學) 수용 양상 - 순자(荀子)에 대한 비판과 이해를 중심으로 -)

  • Yun, Muhak
    • The Journal of Korean Philosophical History
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    • no.25
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    • pp.251-292
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    • 2009
  • In this thesis, the status of Pre-Chin Study of One Hundred Schools in the history of Korean thoughts, and in addition, the reception of $Hs{\ddot{u}}n$ Tzu's ideology and the aspect of its development in Korea were examined. Moreover, the different understandings of $Hs{\ddot{u}}n$ Tzu and their trend of the times were summarized through the intellectuals' collection of works from the end of Goryeo Dynasty and the beginning of Cho Sun Dynasty to 18-19 century. What was revealed through the study can be summed up as the followings. From the age of Three States to the middle part of Goryeo Dynasty, few intellectuals regarded other schools or philosophers among Study of One Hundred Schools as heresies and blamed them. However, since Mencius had been established as the legitimate classical scripture of Confucianism after the end of Goryeo Dynasty and the beginning of Cho Sun Dynasty, other Pre-Chin philosophers including $Hs{\ddot{u}}n$ Tzu had started to be regarded and criticized as heresies. Intellectuals of Goryeo Dynasty and the early Cho Sun Dynasty made various estimates on $Hs{\ddot{u}}n$ Tzu's doctrine of the evilness of human nature, but we can see the understanding of $Hs{\ddot{u}}n$ Tzu became deeper. In the heyday of Neo-Confucianism in Cho Sun Dynasty, the intensity of the criticism on $Hs{\ddot{u}}n$ Tzu by intellectuals rather tended to decrease, compared to that of the early Cho Sun Dynasty, which produced contrary evidence that the world view of Sung Confucianism, based on Mencius' doctrine of the goodness of human nature, had been already established. Also, even the intellectuals criticizing the evilness of human nature positively quoted $Hs{\ddot{u}}n$ Tzu's other ideas in general. In 17th century, there were some arguments to conclude $Hs{\ddot{u}}n$ Tzu as Legalism, but the general trend came to accept Study of One Hundred Schools constructively, challenging the authority of Sung Confucianism, or based on positive school. In 18th century, it can be confirmed that the understanding on $Hs{\ddot{u}}n$ Tzu became broader, and particularly in the historical research of letters, $Hs{\ddot{u}}n$ Tzu's original texts and annotations were used in many ways. In short, the intellectuals' criticism on $Hs{\ddot{u}}n$ Tzu in Cho Sun Dynasty was always grounded on his argument of the evilness of human nature, and furthermore, on connecting it to Legalism, related to the Fenshukengru. On the other hand, it can be said that they generally accepted other ideas of $Hs{\ddot{u}}n$ Tzu positively, except for the idea of the evilness of human nature. However, it's worth paying attention to the fact that those intellectuals who criticized $Hs{\ddot{u}}n$ Tzu could easily meet with the books of Study of One Hundred Schools, and relatively had an open attitude in terms of knowledge.

Complete Genome Sequence and Antimicrobial Activities of Bacillus velezensis MV2 Isolated from a Malva verticillate Leaf (아욱 잎에서 분리한 Bacillus velezensis MV2의 유전체 염기서열 분석과 항균활성능 연구)

  • Lee, Hyeonju;Jo, Eunhye;Kim, Jihye;Moon, Keumok;Kim, Min Ji;Shin, Jae-Ho;Cha, Jaeho
    • Microbiology and Biotechnology Letters
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    • v.49 no.1
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    • pp.111-119
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    • 2021
  • A bacterial strain isolated from a Malva verticillata leaf was identified as Bacillus velezensis MV2 based on the 16S rRNA sequencing results. Complete genome sequencing revealed that B. velezensis MV2 possessed a single 4,191,702-bp contig with 45.57% GC content. Generally, Bacillus spp. are known to produce diverse antimicrobial compounds including bacteriocins, polyketides, and non-ribosomal peptides. Antimicrobial compounds in the B. velezensis MV2 were extracted from culture supernatants using hydrophobic interaction chromatography. The crude extracts showed antimicrobial activity against both gram-positive bacteria and gram-negative bacteria; however, they were more effective against gram-positive bacteria. The extracts also showed antifungal activity against phytopathogenic fungi such as Fusarium fujikuroi and F. graminearum. In time-kill assays, these antimicrobial compounds showed bactericidal activity against Bacillus cereus, used as indicator strain. To predict the type of antimicrobial compounds produced by this strain, we used the antiSMASH algorithm. Forty-seven secondary metabolites were predicted to be synthesized in MV2, and among them, fourteen were identified with a similarity of 80% or more with those previously identified. Based on the antimicrobial properties, the antimicrobial compounds may be nonribosomal peptides or polyketides. These compounds possess the potential to be used as biopesticides in the food and agricultural industry as an alternative to antibiotics.

Effect of Different Liquid Manure Anaerobic Digestates on the Growth and Yield of Rice and the Optimum Application Concentration (혐기소화발효액비의 벼 생육 및 수량에 미치는 영향과 적정 시용량)

  • Byeon, Ji-Eun;Lee, Hong-Ju;Ryoo, Jong-Won;Hwang, Sun-Goo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.66 no.1
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    • pp.97-104
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    • 2021
  • This research examined the effects of different liquid manure based anaerobic digestate on the growth and yield of rice compared to chemical fertilizer. The liquid manure was produced by aerobic fermentation from swine with cow or apple pomace anaerobic digestate and treated at different concentrations. The number of grains per panicle increased in both the liquid manure-treated and chemical fertilizer treated rice. The yield index did not vary significantly between the liquid manure and chemical fertilizer. An increased concentration of liquid manure did not correlate with increases in unhulled rice. However, pH and exchangeable K in the soil increased with an increase in liquid manure. In summary, we suggest a properly applied 100% liquid manure fertilizer can replace chemical fertilizer to reduce our excessive use of inorganic fertilizer.

Daesoon Jinrihoe's Geumgangsan Toseong Training Temple Complex as Appraised through the Hyeonggi Theory in Fengshui (풍수 형기론(形氣論)으로 본 대순진리회 금강산토성수련도장)

  • Shin, Young-dae
    • Journal of the Daesoon Academy of Sciences
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    • v.36
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    • pp.35-78
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    • 2020
  • This study aims to reveal the overall Fengshui figuration and geomantic features of Daesoon Jinrihoe's Geumgangsan (Mt. Geumgang) Toseong Training Temple Complex from the Hyeonggi (Energy of Form) Theory in Fengshui. This study first looked at the mountain landscape viewable from the surface, examined the influence of Qi (Energy) flowing inside it, comprehended the flow of its vitality in terms of its strengths and weaknesses, and gauged the depth of the energy produced from mountain streams to determine fortune and misfortune. There is a special significance to this site due to Sangje's teaching that "⋯ it will be prosperous with 12,000 Dotonggunja (Dao-empowered Sages)," and it is also known as a efficacious grounds for cultivation among ascetics due to it housing the royal mausoleum of Dojeon (interpreted by some as Maitreya). Concerning this, this study explores the geomantic symbolism and growth-supporting land of Geumgangsan Toseong Training Temple Complex as it corresponds to Fengshui theory, and in keeping with this, the topography and conditions are likewise examined. The mountain range and its energy pathways (veins) harmonize with the pure water energy coming from the East Sea. The mountain terrain of Mount Geumgang, and the geomantic location, topography, and energy pathways that influence Daesoon Jinrihoe Geumgangsan Toseong Training Temple Complex are all explored. The Baekdudaegan Mountain Range extends through Mount Geumgang to Sinseonbong Peak, and one range extends to Geumgangsan Toseong Training Temple Complex whereas the other range extends through Sangbong Peak down to Misiryeong Valley and Mount Seorak. Thus, this study demonstrates that Daesoon Jinrihoe has always strongly considered the relationship between its temple complexes and their surrounding environment. The order has always selected locations that exhibit optimal conditions which suit the construction of sacred spaces. The determinations in this paper were made through an academic approach that drew upon various theories of Fengshui while examining Daesoon Jinrihoe's Geumgangsan Toseong Training Temple Complex. The in-depth analysis was specifically based on Hyeonggi Fengshui. At the same time, this study also looked into the surroundings of Geumgangsan Toseong Training Temple Complex. In particular, the mountains and flow of nearby bodies of water were comprehensively examined to show how the surrounding topography corresponds to the principles of Fengshui. An integral approach combining all major theories of Fengshui revealed that Geumgangsan Toseong Training Temple Complex starts from Sinseonbong Peak, and its energy flows through the main mountain range, going through numerous geographical changes of yin and yang. When the range flows down, the water flows accordingly, and where the water whirls, the mountains are shaped accordingly. Eventually, this energy reaches Geumgangsan Toseong Training Temple Complex. From the organic relationship between mountains and bodies of water, which can be said to be the essence of the order of nature, it can be judged that the most prominent geomantic feature of Geumgangsan Toseong Training Temple Complex corresponds to traditional theories of Fengshui in that it forms a configuration wherein optimal water energy supports the Virtuous Concordance of Yin Yang and harmonizes the Blue Dragon with the White Tiger.

Quality characteristics of Halal chicken sausages prepared with biji powder (비지 분말 첨가 Halal 계육 소시지의 조직감 및 항산화특성)

  • Moon, Tae-Hwi;Park, Sun-Min;Yim, Sun-goo;You, Ye-Lim;Han, Jung-Ah
    • Korean Journal of Food Science and Technology
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    • v.54 no.3
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    • pp.334-342
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    • 2022
  • To meet the needs of Muslim consumers, sausages were prepared using Halal-certified chicken thighs and different amounts of biji powder (0, 20, 30, 40, and 50%), and then the properties of the sausages were compared. As the biji powder levels increased, both the moisture content and the pH of the sausages significantly decreased, whereas their fiber content increased. As the biji powder levels increased, the free radical scavenging effect (DPPH, ABTS) and water holding capacity also increased, and the textural properties also improved. When Muslim consumers evaluated the sensory attributes of the sausages prepared in this experiment and those currently on the market, the ones prepared in this experiment were preferred over the market products. Based on the above results, chicken sausage for Muslim consumers could be successfully produced, and their overall quality and antioxidant effects could be improved by the addition of biji powder (up to 30%).

Distributional Characteristics, Population Structures and Fruition Dynamics of Korean Endemic plant, Prunus choreiana H. T. Im (한국특산 복사앵도나무(Prunus choreiana H. T. Im)의 분포특성, 개체군구조 및 결실동태)

  • Kim, Young-Chul;Chae, Hyun-Hee;Son, Sung-Won
    • Korean Journal of Environment and Ecology
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    • v.36 no.2
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    • pp.177-201
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    • 2022
  • Following the adoption of the global plant conservation strategies at the Conference of the Parties for Biodiversity Conservation, diligent actions to achieve each targets are actively carried out. In particular, the need for ecological conservation research to achieve targets 2 and 7 of GSPC-2020 has increased. The priority taxa to accomplish the objectives of GSPC-2020 are rare and endemic plants. In particular, endemic plants with limited distribution in specific regions are evaluated to face a high risk of extinction. To address the necessity to preserve endemic plants, we investigated the distribution of Prunus choreiana H. T. Im, a Korean endemic plant. After that, we examined the vegetational environment of the habitat of P. choreiana and evaluated its population structure. The productivity of its fruits and the effects of pollinators on fruit production were evaluated as well. The fruiting ratio was calculated based on the number of flowers produced. Lastly, we observed the annual growth characteristics of P. choreiana. The habitats of P. choreiana did not show a specific type of vegetation. All of them were located in a limestone area of Gangwon-do in the central Korean Peninsula and occupied a site where the coverage of the tree layer and the sub-tree layer was not high or did not exist. The population structure of P. choreiana contained a high proportion of mature plants capable of producing fruits and a low proportion of seedlings and Juvenile plants. We found that the production of fruits required pollinators and was affected by the performance of each plant. Although P. choreiana produces many flowers, only a maximum of 20% and only 2-6% on average bear fruits. These flowering characteristics may be due to pollinators' low abundance and activity during the flowering season (between mid-March and early April), suggesting that many flowers are needed to attract more pollinators. We rarely observed the re-establishment of seedlings in the population of P. choreiana. Despite that, we predict the population to persist owing to its long lifespan and periodic production of numerous fruits. However, if the tree layer and sub-tree layer in competing status with P. choreiana increase their crown density, they are expected to inhibit the growth of P. choreiana and affect the risk of its extinction. Therefore, the current changes in the vegetational environment of the habitats are expected to decrease the number and extent of P. choreiana in the long term. The results of this study may serve as primary and important data necessary for the achievement of GSPC-2020 objectives.

Revision of Nutrition Quotient for Elderly in assessment of dietary quality and behavior (식사의 질과 식행동 평가를 위한 노인영양지수 개정 연구)

  • Lim, Young-Suk;Lee, Jung-Sug;Hwang, Ji-Yun;Kim, Ki-Nam;Hwang, Hyo-Jeong;Kwon, Sehyug;Kim, Hye-Young
    • Journal of Nutrition and Health
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    • v.55 no.1
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    • pp.155-173
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    • 2022
  • Purpose: This study was undertaken to update the Nutrition Quotient for Elderly (NQ-E), which reflects dietary quality and behavior among Korean older adults. Methods: The first 29 items of the measurable food behavior checklist were obtained from a previous NQ-E checklist, recent literature reviews, and national nutrition policies and recommendations. One-hundred subjects (50 men and 50 women) aged ≥ 65 years living in the Seoul Metropolitan Area, including Gyeonggi Province, completed a pilot survey from March to April 2021. Based on the results of the pilot study, we conducted factor analysis and frequency analysis to determine whether the items of the survey were properly organized and whether the distribution of answers for each evaluation item was properly distributed. As a result, we reduced the number of items on the food behavior checklist and used 23 items for the national survey. Nationwide, 1,000 subjects (472 men and 528 women) aged > 65 years, completed the checklist survey, which was applied using a face-to-face survey method from May to August 2021. The construct validity of the NQ-E 2021 was assessed using confirmatory factor analysis, LISREL. Results: Seventeen food behavior checklist items were selected for the final NQ-E 2021. Checklist items addressed three factors: balance (8 items), moderation (2 items), and practice (7 items). Standardized path coefficients were used as the weights of items to determine nutrition quotients. NQ-E and three-factor scores were calculated according to the weights of questionnaire items. Conclusion: The updated NQ-E 2021 produced by structural equation modelling provides a suitable tool for assessing the dietary quality and behavior of Korean older adults.

A Study on the Origin of Image-Number Theory in Cho Hoik's Yixiangshuo (조호익(曺好益) 『역상설(易象說)』의 상수학적 연원)

  • Im, Jae-kyu
    • Journal of the Daesoon Academy of Sciences
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    • v.38
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    • pp.183-208
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    • 2021
  • In order to examine the origin of Image-Number Theory in Cho Hoik (曺好益)'s Yixiangshuo (易象說), it is necessary to review Hu Yigui (胡一桂)'s Zhouyi Benyi Fulu Zuanzhu (周易本義附録纂注). Hu Yigui based his work on Zhu Xi's Zhouyi Benyi, he took related contents such as the Zhu Xi's writings and phrases and organized them into a fulu (附録), and he collected commentaries that matched the meaning of Zhouyi Benyi among the theories of many Confucian scholars and produced a zuanzhu (纂注). In addition to these, there are 'Yuwei (愚謂)' and 'Yuan (愚案)' which allowed him to add his own opinion. The system of Hu Yigui's Zhouyi Benyi Fulu Zuanzhu almost coincides with Cho Hoik's Yi-ological writing system. In other words, Cho Hoik appears to have written Yizhuan Bianjie (易傳辨解) and Zhouyi Shijie (周易釋解) as a fulu and zuanzhu of Zhouyi Benyi Fulu Zuanzhu. And there is Yixiangshuo which corresponds to 'Yuwei' and 'Yuan' of Zhouyi Benyi Fulu Zuanzhu. Yixiangshuo was not originally an independent Yi-ological book, but was compiled by later generations from what was recorded in the form of the head notes of Zhouyi (周易). Thus, Yixiangshuo takes almost the same form as the 'Yuwei' and 'Yuan' of Zhouyi Benyi Fulu Zuanzhu. In addition, Cho Hoik's Yixiangshuo cites many contents from 'Yuwei' and 'Yuan' of Zhouyi Benyi Fulu Zuanzhu. On the other hand, in order to examine the origin of Image-Number Theory in Cho Hoik's Yixiangshuo, the Yi-ology of Zhu Zhen (朱震) cannot be overlooked. This is true not only due to the fact that Yixiangshuo is quoting Zhu Zhen. The more significant reason is Yixiangshuo is a fundamental aspect of Zhu Zhen's Yi-ology. As demonstrated in the main body of this article, the methodology of Image-Number Theory in Yixiangshuo and its counterpart in Hanshang Yizhuan (漢上易傳) are almost identical. In conclusion, the origin of Image-Number Theory in Cho Hoik's Yixiangshuo can be found in both the Hu Yigui's Zhouyi Benyi Fulu Zuanzhu and Zhu Zhen's Hanshang Yizhuan. In particular, it can be said that its origin can be found in both the 'Yuwei' and 'Yuan' of Zhouyi Benyi Fulu Zuanzhu and the methodlogy of Image-Number Theory in Hanshang Yizhuan.