• The Korean Journal of Pesticide Science
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• v.5 no.4
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• pp.50-56
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• 2001

In vitro experiments, several fungicides including prochloraz, tebuconazole, fluazinam, fludioxonil, and iminoctadine-triacetate showed more than 85% inhibition of mycelial growth of Pestalotiopsis theae (SP-3). Dichlofluanid and chlorothalonil inhibited mycelial growth at the rate of 10 and 33%, however benomyl did not inhibit mycelial growth of the fungus. Minimum inhibitory concentration(MICs) of iminictadine-triacetate on the mycelial growth of SP-3 isolate was $10{\mu}g/m{\ell}$, but that of P. theae $\underline{MAFF}$ 752002 and P. longiseta $\underline{MAFF}$ 752001 was $1{\mu}g/m{\ell}$. MIC of benomyl, chlorothalonil, dichlorofluanid was $1,000{\mu}g/m{\ell}$, and that of fludioxonil, fluazinam, tebuconazole was $10{\mu}g/m{\ell}$. Conidial germination was inhibited more than 80% in tile medium which contained $1{\mu}g/m{\ell}$ of prochloraz, tebuconazole, fluazinam, fludioxonil, and minoctadine-triacetate. Control values of benomyl, chlorothalonil and dichlofluanid were 40, 60 and 30%, respectively. The controlling effect of iminoctadine-triacetate ($10{\mu}g/m{\ell}$) aganist P. theae (SP-3) in leaf disc test was more than 93%, but benomyl and dichlofluanid could not control leaf blight disease caused by P. theae (SP-3). Tebuconazole, fluazinam, fludioxonil, and iminoctadine-triacetate showed more than 94% of protective and curative effect of leaf blight of sweet persimmon, while, benomyl and dichlofluanid did not show any control effect of the disease.

• The Korean Journal of Pesticide Science
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• v.17 no.2
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• pp.133-139
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• 2013

To investigate the sensitivity of Sclerotium cepivorum causing garlic white rot to 5 fungicides, including prochloraz, tebuconazole, flutolanil, iminoctadine and isoprothiolane, 147 isolates isolated from infected garlics from 2008 to 2009 through a single sclerotium isolation were screened. While each mean value of $EC_{50}$ (effective concentration reducing mycelial growth by 50%) of S. cepivorum isolates collected in 2008 to each fungicide was 0.054, 0.012, 23.189, 0.901, and $21.362{\mu}g\;mL^{-1}$, that of 2009 isolates were 0.030, 0.020, 10.367, 1.684, $33.406{\mu}g\;mL^{-1}$. There was a difference in mean value of $EC_{50}$ of S. cepivorum according to regions isolated. $EC_{50}$ values of S. cepivorum isolated in Goheung to flutolanil and isoprothiolane were 14.468 and $24.653{\mu}g\;mL^{-1}$, respectively, which was lower than those of Seosan and Taeahn. Isolates from Taeahn showed the lowest $EC_{50}$ value to prochloraz as $0.008{\mu}g\;mL^{-1}$. In addition, we could not find any resistant isolates to fungicides tested. The $EC_{50}$ values in this study will be used in a fungicide resistance monitoring program to determine whether shifts in sensitivity to fungicides included into different groups are occurring in S. cepivorum populations.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.4
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• pp.287-293
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• 1991

The root-mat formation of infant rice seedlings (8- to 10-day-old) is sometime not enough for machine transplanting because of the too-short nursery period. This experiment was conducted to elucidate the effect of metalaxyl seed-soaking treatment in the mixed solution of metalaxyl, seed disinfectant and insectcides on the root-mat formation of infant rice seedling in machine transplanting. The rice seeds of Odaebyeo were soaked in the mixed solution of metalaxyl, prochloraz and insectcides. with the recommended concentrations for 24 hours at room temperature. Seeding rate was 220g per seed tray (30 x 60 x 3cm). Metalaxyl (25% wettable powder), a fungicide. was used in 1. 000 times dilution as a promoting substance on the root-mat formation. Generally, the metalaxyl-treated seeds markedly increased the root number and length, and rooting activity of infant rice seedlings as compared with the control, thus the root-mat formation was excellent. When the rice seeds were sterilized by the mixed solution of prochloraz and insectcides, metalaxyl could be used together for increasing root-mat rormation of infant rice seedlIngs due to no interaction among agro-chemicals used. Seedling damping-off and physiological seedling rot were also controled in the seedlings of metalaxy-treated seeds. The root-mat of metalxyl-treated seeds had higher hardness and tension than control in terms of physical properties.

• Korean Journal of Environmental Agriculture
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• v.37 no.1
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• pp.34-40
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• 2018

BACKGROUND: In order to select a fungicide that can effectively control anthracnose disease in Japanese plum fruit, mycelial growth inhibition effect and spore germination inhibition effect of six fungicides were tested in vitro against six isolates of Colletotrichum acutatum and five isolates of C. gloeosporioides that were isolated from diseased Japanese plum fruit. METHODS AND RESULTS: Inhibitory effects of fungicides on mycelial growth were investigated after inoculating each isolate on potato dextrose agar amended with four discriminatory concentrations of each fungicide for 7 days at $25^{\circ}C$. For spore germination inhibitory effect, each isolate of the Colletotrichum spp. was cultured in potato dextrose agar for 7-14 days at $25^{\circ}C$. After adjusting the concentration of spores of each isolate to $1{\times}10^6mL^{-1}$ by diluting with 0.025% PDB, the spore suspension was mixed with each fungicide (1:4, v/v), and $60{\mu}L$ aliquots were dispensed to sterile hole slide glass. Hole slide glasses were placed in a humidified box and incubated for 15 hours at $25^{\circ}C$. Then, spore germination was observed under an optical microscope. At recommended concentration of fungicide prochloraz manganese showed the highest mycelial growth inhibitory effect and dithianon showed the lowest mycelial growth inhibition. The $EC_{50}$ values for the inhibition of spore germination by dithianon and pyraclostrobin were $0.069-0.126{\mu}g/mL$ and $0.37-1.59{\mu}g/mL$, respectively. Although benomyl, prochloraz manganese, azoxystrobin, and tebuconazole did not inhibit the spore germination, they appeared to restrain mycelial growth by abnormal growth of germ tube and mycelium after germination. CONCLUSION: Dithianon seemed to have preventive effect. Prochloraz manganese, azoxystrobin, and tebuconazole were likely to have control effect. Pyraclostrobin is considered to have both preventive and control effect against anthracnose disease of Japanese plum fruit.

• The Korean Journal of Mycology
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• v.38 no.1
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• pp.75-79
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• 2010

A total of 25 isolates of Fusarium fujikuroi were obtained from diseased rice plants in Korea from 2006 to 2007 to assess their resistance against fungicides prochloraz and benomyl + thiram. Minimal inhibitory concentration (MIC) values of F. fujikuroi isolates were examined by agar dilution method. Most of the isolates were sensitive to the fungicides. Out of 25 isolates, six were resistant to prochloraz and three to benomyl + thiram. In addition, the isolates CF245, CF249 and CF337 showed resistant to both fungicides. The progenies ($F_1$ isolates) obtained through two different crosses between sensitive parental isolates(CF202, CF232 and CF179) and resistant parental isolate (CF337) were evaluated for their mycelial growth at different temperatures and resistance against fungicides. Mycelial growth rate of $F_1$ isolates originated from CF202 $\times$ CF232 was similar to the parental isolates. However mycelial growth rate of $F_1$ isolates originated from CF179 $\times$ CF337 was faster than their parent isolates. In case of prochloraz, distribution ratio of sensitivity(S) to resistance(R) against to the fungicide of $F_1$ isolates originated from CF202 $\times$ CF232 and CF179 $\times$ CF337 was 86 : 14 and 78 : 22, respectively. In case of benomyl+thiram, all the $F_1$ isolates originated from CF202 $\times$ CF232 were sensitive to the fungicide, however ratio of sensitivity(S) to resistance(R) against to the fungicide of $F_1$ isolates originated from CF179 $\times$ CF337 was 35 : 65.

• Korean Journal of Agricultural Science
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• v.31 no.1
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• pp.45-52
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• 2004

Bakanae Disease is an abnormal plant growth caused by Gibberella fujikuroi. The infected plants several inches taller than normal plants in seedbed and field. To control bakanae disease, we made a seed disinfectant from garlic extract. And we isolated allicin($C_6H_{10}OS_2$) from garlic extract and the content was 0.62%. In order to develop efftive seed disinfectant from Garlic extract, we compared it with three seed disinfectants(benomyl, prochloraz, fludioxonil). Prochloraz, fludioxonil and Garlic wettable powder controlled Bakanae disease of rice very well, while benomyl did not suppress the disease enough. Water temperature was turned to be an important factor for controlling the disease by treating seed disinfectants. It was confirmed that the seed disinfectant from garlic extract can be used as non-toxic agricultural medicines.

• 한국균학회소식:학술대회논문집
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• 2014.10a
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• pp.25-25
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• 2014

Three species of Fusarium, F. fujikuroi, F. verticillioides and F. proliferatum, are known to be associated with bakanae disease of rice [1, 2]. F. fujikuroi infects rice flowers and survive in endosperm and embryo of the seeds. Infected seed is an important source of primary inoculum of pathogens [3]. Seeds of rice (Oryza sativa cv. Boramchan) collected from bakanae-infected field were found to be 96% infected with Fusarium sp., 52% with F. fujikuroi, 42% with F. verticillioides, and 12% with F. proliferatum as determined by incubation method and species-specific PCR assays. F. fujikuroi was detected at lemma/palea, endosperm and embryo whereas F. verticillioides and F. proliferatum were recovered only from lemma/palea by means of component plating test. Seed disinfection methods have been developed to control bakanae disease and prochloraz has been most widely used for rice seeds. Two chemicals formulated with prochloraz (PC 1) and prochloraz + hexaconazole (PC 2) that inhibit biosynthesis of ergosterol strongly reduced the incidence of Fusarium spp. on selective media to 4.7% and 2.0%, respectively. Disease symptoms of rice seedlings in nursery soil were alleviated by chemical treatment; seedlings with elongated leaves or wide angle between leaf and stem were strikingly reduced from 15.6 to 3.2% (PC 1) and 0 (PC 2), stem rots were reduced from 56.9 to 26.2% (PC 1) and 32.1% (PC 2), and normal seedling increased from 0.4 to 13.3% (PC 2). Prochloraz has some disadvantages and risks such as the occurrence of tolerant pathogens [4] and effects on the sterol synthesis in animals and humans [5]. For these reasons, it is necessary to develop new disinfection method that do not induce fungal tolerance and are safe to humans and animals. Chlorine dioxide ($ClO_2$), that is less toxic, produces no harmful byproducts, and has high oxidizing power, has been reported to be effective at disinfection of several phytopathogenic fungi including Colletotrichum spp. and Alternaria spp. [6]. Gaseous $ClO_2$ applied to rice seeds at a concentration of 20 ppm strongly suppressed mycelial growth of Fusarium fujikuroi, F. verticillioides and F. proliferatum. The incidence of Fusarium spp. in dry seed with 8.7% seed moisture content (SMC) tended to decrease as the concentration of $ClO_2$ increased from 20 to 40 ppm. Applying 40 ppm $ClO_2$ at 90% relative humidity, incidence was reduced to 5.3% and resulted in significant reduction of disease symptoms on MS media. In nursery soil, stem rot was reduced from 56.9 to 15.4% and the number of normal seedlings increased from 0.4 to 25.5%. With water-soaked seeds (33.1% SMC) holding moisture in the endosperm and embryo, the effectiveness of disinfection using $ClO_2$ increased, even when treated with only 20 ppm for four hours. This suggests that moisture was a key element for action of $ClO_2$. Removal of the palea and lemma from seeds significantly decreased the incidence of Fusarium spp. to 3.0%. Seed germination appeared to decrease slightly by water-soaking at $30^{\circ}C$ because of increased SMC and by physical damage of embryos from hulling. These results indicate that the use of gaseous $ClO_2$ was effective as a means to disinfect rice seeds infected with Fusarium spp. and that moisture around the pathogens in the seed was an important factor for the action of $ClO_2$. Further investigations should be conducted to ascertain the best conditions for complete disinfection of Fusarium spp. that infect deep site of rice seeds.

• Horticultural Science & Technology
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• v.19 no.4
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• pp.591-595
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• 2001

This study was conducted to find out the proper chemicals and doses for controlling the bulb disease of Lilium Oriental hybrids 'Capablanca' and 'Marco Polo' cultivars during storage, and to ascertain any disadvantage of the treatment on shoot growth and flower development. Bulbs of 'Casablanca' and 'Marco Polo' cultivars were dipped in some fungicide solutions, including azoxystrobin, benomyl+thiram, and prochloraz, and culture broth of antagonistic bacteria for one hour. The rot was apparently inhibited by azoxystrobin and prochloraz solution dippings and it was not by benomyl+thiram in bulbs produced domestically. To improve physiological integrity, calcium chloride was treated. However, this treatment had no positive effects on bulb rot of lily. When bulbs were dipped in one-fifth of diluted broth. But the bulb rot was inhibited effectively as compared with untreated during storage. Benomyl+thiram and that with calcium inhibited the sprouting in 'Casablanca' and 'Marco Polo' cultivars during cultivation. Calcium and benomyl+thiram treatments decreased growth of leaf and flower but with no statistically significant difference. Other fungicidal materials treated to bulbs showed no effect on vegetative growth and flower quality.

• Journal of Applied Biological Chemistry
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• v.62 no.1
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• pp.11-17
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• 2019

Strawberries are one of the main commodities in Korea and have been exported over 40 million dollar. Because the strawberry cultivation using hydroponics has increased, treatment of pesticide by drenching draw interest recently. However, detailed researches for drenching treatment of pesticide are limited, which results in difficulties in proper pesticide applications in agricultural fields. To activate use of drenching and improve safety in agricultural products, In this study, time-dependent residual characteristic of pesticides were compared with between different applications of pesticides in strawberries. The characteristics of azoxystrobin, prochloraz and thiamethoxam were investigated with drenching treatment at different applications: the time of treatment, concentration etc. at hydroponic cultivation for 40 days. Azoxystrobin and prochloraz were not detected at 14 day after application. Thiamethoxam was detected from 0.02 to 0.85 mg/kg. Crop uptake of pesticides was strongly affected with octanol-water partition coefficient and solubility in water. Residual amount in crops are highly dependent on the concentration of active ingredient of pesticides.

• Research in Plant Disease
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• v.28 no.3
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• pp.122-131
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• 2022

Colletotrichum acutatum s. lat. 20JDS8 sensitive and 20CDJ6 resistant to pylaclostrobin were used to investigate the cross-resistance with fungicides belonging to the strobilurins and the characteristics of fungicidal controlling activities with different mechanisms against the isolate resistant to the fungicide. The resistant isolate of 20CDJ6 also showed the resistance to azoxystrobin, trifloxystrobin, and kresoxim-methyl, suggesting that there is a cross-resistance relationship. All fungicides with different action mechanisms inhibited mycelial growth of both susceptible and resistant isolates of C. acutatum s. lat., but their disease control effects in fruits were different according to the fungicides. The disease control effect of isopyrazam against 20JDS8 and 20CDJ6 was very low, and fluazinam showed a control effect of 91.9% and 88.1% against 20JDS8 and 20CDJ6 only when it was treated before inoculation by spraying spore suspensions on pepper fruits without wounds. Tebuconazole and prochloraz effectively inhibited not only the mycelial growth of 20JDS8 and 20CDJ6 on potato dextrose agar medium, but also disease incidence in red pepper fruits. As a result of this study, C. acutatum s. lat. 20CDJ6 resistant to pyraclostrobin showed cross-resistance with other strobilurin fungicides. In addition, we think that fluazinam, tebuconazole, and prochloraz can be recommended as alternative fungicides for the control of red-pepper pyranthracnose pathogens resistant pyraclostrobin. However, fluazinam can be effective only if it is treated protectively before the occurrence of the disease.