• Title/Summary/Keyword: Probiotics

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Prevention of Alcoholic Liver Disease by Using Probiotics (프로바이오틱스 섭취를 통한 알코올성 간 질환의 완화)

  • Lee, In Ok;Kim, Sae Hun
    • Journal of Dairy Science and Biotechnology
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    • v.32 no.1
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    • pp.1-6
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    • 2014
  • Probiotics have been extensively studied for their beneficial effects on human health. In particular, Lactobacillus and Bifidobacterium strains have gained considerable attention as major groups of probiotic bacteria that improve gastrointestinal health. However, emerging evidence suggests that probiotics offer benefits beyond those observed in the gut recent studies suggest that probiotics and/or their components exert favorable effects on alcoholic liver disease (ALD) pathogenesis such as decreasing intestinal permeability, inhibiting pathogenic bacteria growth, increasing the activity of alcohol metabolism enzymes, modulating the adaptive immune system, and suppressing fatty acid synthesis genes. In this review, we discuss the results of in vivo and in vitro studies that have examined the use of probiotics to prevent ALD, primarily focusing on those that explore the cellular and molecular mechanisms underlying the activities of promising probiotic strains. The evidence presented in this review could help in screening for probiotic strains that have protective effects in ALD patients and in further elucidating the mechanisms of their actions.

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Optimization of the Growth Rate of Probiotics in Fermented Milk Using Genetic Algorithms and Sequential Quadratic Programming Techniques

  • Chen, Ming-Ju;Chen, Kun-Nan;Lin, Chin-Wen
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.6
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    • pp.894-902
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    • 2003
  • Prebiotics (peptides, N-acetyglucoamine, fructo-oligosaccharides, isomalto-oligosaccharides and galactooligosaccharides) were added to skim milk in order to improve the growth rate of contained Lactobacillus acidophilus, Lactobacillus casei, Bifidobacterium longum and Bifidobacterium bifidum. The purpose of this research was to study the potential synergy between probiotics and prebiotics when present in milk, and to apply modern optimization techniques to obtain optimal design and performance for the growth rate of the probiotics using a response surface-modeling technique. To carry out response surface modeling, the regression method was performed on experimental results to build mathematical models. The models were then formulated as an objective function in an optimization problem that was consequently optimized using a genetic algorithm and sequential quadratic programming approach to obtain the maximum growth rate of the probiotics. The results showed that the quadratic models appeared to have the most accurate response surface fit. Both SQP and GA were able to identify the optimal combination of prebiotics to stimulate the growth of probiotics in milk. Comparing both methods, SQP appeared to be more efficient than GA at such a task.

A Review on Effects of Probiotics in Atopic Dermatitis (아토피 피부염에서 프로바이오틱스 치료 유효성에 관한 문헌적 고찰)

  • Hong, Soo-Jeong;Chong, Myong-Soo;Ahn, Sung-Hun
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.33 no.1
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    • pp.25-44
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    • 2020
  • Objective : The purpose of this study is to investigate the use of probiotics for cure of atopic dermatitis and suggest for the better method of studies in order to seek more effective treatment. Methods : We searched from Korean Traditional Knowledge Portal(KTKP), Reserach Information Sharing Service(RISS), National Discovery for Science Leader(NDSL) and PubMed. We selected among the papers published from 2005 to 2019 using terms related to 'Atopic dermatitic & Allergy diseases & Probiotics'. Results : 41 papers were selected from the database worldwidely. In these collected 41 related theses, there were 14 experimental studies(34.1%), 16 clinical studies( 39.0%), 11 review studies (26.9%). 23 papers(76.7%) insisted that the effects is but 9 papers(30%) said not effective. Conclusion : The effects of probiotics in atopic dermatitis is investigated positively, however, we have to consider the methods to approach the result including the sort, amount of probiotics, how to culture, how to eat and so on.

Inhibitory Effects of Lactobacillus plantarum Q180 on Lipid Accumulation in HepG2 Cells

  • Chu, Jaeryang;Joung, Hyunchae;Kim, Byung-Kook;Choi, In-Suk;Park, Tae-Sik
    • The Korean Journal of Food And Nutrition
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    • v.32 no.6
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    • pp.738-744
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    • 2019
  • Recently, the prevalence of hyperlipidemia has been increasing, and consequently, the need to identify safe and effective treatments to control this chronic disease has also increased. The beneficial effects of probiotics have been revealed by several studies over the past few years, including their effects on hypertriglyceridemia. However, the mechanisms of action of probiotics are still unclear. The anti-obesity effects of Lactobacillus plantarum Q180 on lipid accumulation have already been demonstrated using an in vitro HepG2 cell model, and therefore, we investigated its efficacy and mechanism of action. Lipid accumulation was induced in HepG2 cells by palmitic acid treatment and then the cells were incubated with L. plantarum Q180 lysate or supernatant to investigate changes in lipid accumulation and expression of lipid metabolism-related genes. The results showed that the L. plantarum Q180-treated group exhibited significantly lower levels of lipid accumulation and mRNA expression of lipid synthesis- and adipogenesis-related genes than the palmitic acid-treated group did. These results indicate that L. plantarum Q180 may contribute to alleviating hypertriglyceridemia by inhibiting lipid synthesis.

Bioactive Molecules Produced by Probiotics to Control Enteric Pathogens (프로바이오틱스가 생산하는 생리활성 물질의 장내 유해균 억제 효과)

  • Lim, Kwang-Sei;Griffiths, Mansel W.;Park, Dong June;Oh, Sejong
    • Journal of Dairy Science and Biotechnology
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    • v.32 no.2
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    • pp.141-145
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    • 2014
  • There is a burgeoning number of products on the market that contain probiotics, but do they do you any good? What exactly are probiotics? They have been defined as living organisms that, when ingested in sufficient quantities, provide health benefits beyond basic nutrition. They are often referred to as "friendly bacteria" or "good bacteria." Probiotics have been claimed, amongst other things, to (i) reduce the incidence of colon cancer and other diseases of the colon, such as IBS, (ii) stimulate the immune system, (iii) have anti-hypertensive and anti-cholesterolemic properties, (iv) mitigate against the effect of antibiotics on the intestinal microbiota, and (v) protect against gastrointestinal infections. However, the scientific basis for many of these claims is not well-established. Indeed, the European Food Safety Authority has denied the use of several health claims associated with probiotics, particularly those related to mitigation of diarrhea following consumption of antibiotics. Thus, there is a need for research on the mechanisms of action of probiotics. We have been mainly interested in the use of probiotics to control enteric infections. There are several possible modes of action to explain how probiotics may protect the host from enteric pathogens, including competitive exclusion and immunomodulation. We have shown that probiotics produce bioactive molecules that interfere with bacterial cell-cell communication (also called quorum sensing), and this results in a down-regulation of virulence genes that are responsible for attachment of the pathogen to the gastrointestinal epithelium. These bioactive molecules act on a variety of bacteria, including enterohemorrhagic and enterotoxigenic Escherichia coli, Salmonella, Clostridium difficile and Clostridium perfringens, and there is evidence that they can inhibit the formation of biofilms by Listeria monocytogenes. These bioactive molecules, which are peptidic in nature, can exert their effects not only in vitro but also in vivo, and we have shown that they mitigate against E. coli O157:H7 and Salmonella in mice and Salmonella and E. coli K88 infections in pigs. They can be delivered in foods such as yoghurt and maintain their activity.

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Immunomodulatory Properties of Lactobacillus plantarum NC8 Expressing an Anti-CD11c Single-Chain Fv Fragment

  • Liu, Jing;Yang, Guilian;Gao, Xing;Zhang, Zan;Liu, Yang;Yang, Xin;Shi, Chunwei;Liu, Qiong;Jiang, Yanlong;Wang, Chunfeng
    • Journal of Microbiology and Biotechnology
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    • v.29 no.1
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    • pp.160-170
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    • 2019
  • The lactic acid bacteria species Lactobacillus plantarum (L. plantarum) has been used extensively for vaccine delivery. Considering to the critical role of dendritic cells in stimulating host immune response, in this study, we constructed a novel CD11c-targeting L. plantarum strain with surface-displayed variable fragments of anti-CD11c, single-chain antibody (scFv-CD11c). The newly designed L. plantarum strain, named 409-aCD11c, could adhere and invade more efficiently to bone marrow-derived DCs (BMDCs) in vitro due to the specific interaction between scFv-CD11c and CD11c located on the surface of BMDCs. After incubation with BMDCs, the 409-aCD11c strain harboring a eukaryotic vector pValac-GFP could lead to more efficient expression of GFP compared with wild-type strains shown by flow cytometry analysis, indicating the enhanced translocation of pValac-GFP from L. plantarum to BMDCs. Similar results were also observed in an in vivo study, which showed that oral administration resulted in efficient expression of GFP in both Peyer's patches (PP) and mesenteric lymph nodes (MLNs) within 7 days after the last administration. In addition, the CD11c-targeting strain significantly promoted the differentiation and maturation of DCs, the differentiation of $IL-4^+$ and $IL-17A^+$ T helper (Th) cells in MLNs, as well as production of $B220^+$ $IgA^+$ B cells in the PP. In conclusion, this study developed a novel DC-targeting L. plantarum strain which could increase the ability to deliver eukaryotic expression plasmid to host cells, indicating a promising approach for vaccine study.

Influence of Probiotics Supplementation on Egg Quality and Excretal Noxious Gas in Laying Hens (산란계에 있어 생균제의 첨가가 계란품질 및 배설물내 유해가스 함량에 미치는 영향)

  • Hong, J.W.;Kim, I.H.;Kwon, O.S.;Han, Y.K.;Lee, S.H.
    • Journal of Animal Science and Technology
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    • v.44 no.2
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    • pp.213-220
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    • 2002
  • This study was conducted to investigate the effects of feeding probiotics on the egg quality and excretal noxious gas in laying hens. One hundred forty four, 36 weeks old ISA brown commercial layer, were employed in a 28 d feeding trial with a 7 d adjustment period. Dietary treatments are 1) control(basal diet), 2) PB0.3(basal diet+0.3% probiotics), 2) PB0.6(basal diet+0.6% probiotics). For overall period, hen-day egg production, egg weight and egg shell breaking strength tended to increase (P>0.05) by dietary probiotic supplementation. Egg shell thickness was improved by supplementation of probiotics(linear effect, P$<$0.01). Diets PB0.3 and PB0.6 improved the yolk color compared to control diet(linear effect, P$<$0.02). As supplementation level of probiotics increased in the diets, egg yolk index tended to increase(linear effect, P$<$0.04). $NH_3$-N concentration in excreta fed PB0.6 diet was significantly (P$<$0.04) lower than either control or PB0.3 diet. In conclusion, supplementing probiotics to a Corn-SBM diet for laying hens increased egg shell thickness, yolk color and decreased egg yolk index, and decreased excretal $NH_3$-N concentration.

Effects of Dietary Supplementation of Complex Probiotics on Performance in Laying Hens (복합생균제의 급여가 산란계의 생산성에 미치는 영향)

  • 나재천;김태호;서옥석;유동조;김학규;이상진;김상호;하정기;김재황
    • Korean Journal of Poultry Science
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    • v.30 no.3
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    • pp.145-150
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    • 2003
  • A feeding trial was carried out to investigate the effect of dietary complex probiotics on performance. egg qualities and intestinal microflora in laying hens. Three hundred twenty ISA Brown laying hens, 34 weeks of age, were randomly allotted to four dietary treatments containing 0, 0.1, 0.2 and 0.4% complex probiotics for 12 weeks. There were four replicates per treament. Total egg production, soft and broken egg number tended to improve as dietary complex probiotics increased, but was not significantly different. Average egg weight was significantly higher in the 0.1% and 0.2% complex probiotics than the control(P<0.05). Daily egg mass also increased by adding complex probiotics compared to that of control, but was not statistically different. No significant difference was found in feed intake and feed conversion ratio. Eggshell breaking strength and thicknes were not significantly different, whereas yolk color was significantly lower in the supplemental 0.2% probiotics than the comtrol at 12 weeks of age(P<0.05). There was no significant difference in Haugh unit. Total number of cecum Lactobacillus and naerobes were significantly higher in the complex probiotics than control(p.0.05). However, the number of ileal Lactobacillus and naerobe were not significantly different. It was concluded that dietary complex probiotics could improve the egg weight and intestinal beneficial microbes.

A New Method of Producing a Natural Antibacterial Peptide by Encapsulated Probiotics Internalized with Inulin Nanoparticles as Prebiotics

  • Cui, Lian-Hua;Yan, Chang-Guo;Li, Hui-Shan;Kim, Whee-Soo;Hong, Liang;Kang, Sang-Kee;Choi, Yun-Jaie;Cho, Chong-Su
    • Journal of Microbiology and Biotechnology
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    • v.28 no.4
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    • pp.510-519
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    • 2018
  • Synbiotics are a combination of probiotics and prebiotics, which lead to synergistic benefits in host welfare. Probiotics have been used as an alternative to antibiotics. Among the probiotics, Pediococcus acidilactici (PA) has shown excellent antimicrobial activity against Salmonella Gallinarum (SG) as a major poultry pathogen and has improved the production performances of animals. Inulin is widely used as a prebiotic for the improvement of animal health and growth. The main aim of this study was to investigate the antimicrobial activity of inulin nanoparticle (IN)-internalized PA encapsulated into alginate/chitosan/alginate (ACA) microcapsules (MCs) for future in vivo application. The prepared phthalyl INs (PINs) were characterized by DLS and FE-SEM. The contents of phthal groups in the PINs were estimated by $^1H-NMR$ measurement as 25.1 mol.-%. The sizes of the PINs measured by DLS were approximately 203 nm. Internalization into PA was confirmed by confocal microscopy and flow cytometry. The antimicrobial activity of PIN-internalized probiotics encapsulated into ACA MCs was measured by coculture antimicrobial assays on SG. PIN-internalized probiotics had a higher antimicrobial ability than that of ACA MCs loaded with PA/inulin or PA. Interestingly, when PINs were treated with PA and encapsulated into ACA MCs, as a natural antimicrobial peptide, pediocin was produced much more in the culture medium compared with other groups with inulin-loaded ACA MCs and PA encapsulated into ACA MCs.

Studies on the Oral Disease Improvement Effects of Probiotics : A Review (프로바이오틱스의 구강질병 개선에 관한 연구 고찰)

  • Chung, Sung-Kyun
    • Journal of Digital Convergence
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    • v.17 no.6
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    • pp.315-326
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    • 2019
  • This study is a review of research articles on the characteristics of probiotics. Probiotics are effective at ameliorating immune disease, alleviating glucose intolerance, and improving constipation and diarrhea. Furthermore, they have anticancer and antitumor effects, preventive effects against cardiovascular disease, antidiabetic effects, antioxidant effects, antibacterial effects, and they produce useful metabolites. It has been demonstrated that oral probiotics are effective in eliminating halitosis and forming a favorable oral ecosystem, by creating an environment that is not readily inhabited by harmful bacteria, such as Streptococcus mutans, which produces lactic acid and causes tooth decay, or Porphyromonas gingivalis, which causes gum disease.As a result, oral probiotics are being considered not only as therapeutics against diseases, but also as preventive agents for the maintenance of a healthy oral balance. In spite of some limitations, clinical trials are currently underway, and this study can provide evidence to support the use of probiotics to improve oral health conditions, including dental caries, periodontal disease, and Candida colonization.