• 제목/요약/키워드: Probes

검색결과 1,237건 처리시간 0.024초

전기화학적 에칭을 이용한 텅스텐 미세 탐침 가공 (Fabrication of Tungsten Probe using Electro-Chemical Etching)

  • 인치현;김규만;주종남
    • 한국정밀공학회지
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    • 제18권2호
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    • pp.111-118
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    • 2001
  • Tungsten probe is the most important part of a probe card, which is widely used for the performance test of wafer chips. Electro chemical etching becomes an exclusive choice for mass production of the tungsten probes. In the mass production, not only the shape of the probe but also the shape distribution of machined probes is important. A new method is proposed for the mass production of the tungsten probes. Tungsten wires are separated by a distance, and dipped into electrolyte. The dipping rate is controlled to shape the probes. Several experimental tests are performed to study the machining characteristics. From the test results, machining parameters including electrical conditions and anode position showed significant influences on the shape, repeatability, precision and quality of sharp tips.

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Small-molecule probes elucidate global enzyme activity in a proteomic context

  • Lee, Jun-Seok;Yoo, Young-Hwa;Yoon, Chang No
    • BMB Reports
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    • 제47권3호
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    • pp.149-157
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    • 2014
  • The recent dramatic improvements in high-resolution mass spectrometry (MS) have revolutionized the speed and scope of proteomic studies. Conventional MS-based proteomics methodologies allow global protein profiling based on expression levels. Although these techniques are promising, there are numerous biological activities yet to be unveiled, such as the dynamic regulation of enzyme activity. Chemical proteomics is an emerging field that extends these types proteomic profiling. In particular, activity-based protein profiling (ABPP) utilizes small-molecule probes to monitor enzyme activity directly in living intact subjects. In this mini-review, we summarize the unique roles of smallmolecule probes in proteomics studies and highlight some recent examples in which this principle has been applied.

SYNTHESIS AND APPLICATION OF NEW SPIN PROBES

  • Kim, S.D.;Freeman, H.S.;Mcgregor, R.
    • 한국섬유공학회:학술대회논문집
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    • 한국섬유공학회 1990년도 제2차 학술발표초록집
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    • pp.67-67
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    • 1990
  • Three non-ionic and two anioinic spin probes, differing in size and substituent, were synthesized. Their mobility in dried nylon 6 film was investicated by the spin probe technique using electron spin resonance spectrometer. When the size of a spin probe was large and the interaction between the probe molecules and polymer chains existed, the mobility of spin probes decreased. From Arrhenius plots of rotational correlation time, one discontinuity point ($T_d$) was determined. The activation energies for rotation below and above $T_d$ were discussed in terms of the mode of probe rotation. Three spin probes could be viewed as azo dyes having a built-in nitroxide radical. Photolysis of them in dimethylformamide and in nylon 6 film was performed by exposure to 254 nm UV light in the presence of air. It was found that dyes having a built-in nitroxide radical showed better photostability than dyes derived from ${\bata}-naphthol$..

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Fuarium oxysporum의 유연관계 분석을 위한 Recombinant DNA의 Probe로서의 이용 가능성 (Potential Applications of Recombinant DNA Probes for Relatedness Analysis of Fusarium oxysporum)

  • 김홍기;김영태;유승헌
    • 한국식물병리학회지
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    • 제10권1호
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    • pp.1-6
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    • 1994
  • Randomly chosen recombinant clones of Fusarium oxysporum were analysed to select useful probes for relatedness analysis of Fusarium oxysporum. Genomic DNA of F. oxysproum f. sp. cubense, digested with HindIII, was ligated to pUC118 and used to transform Escherichia coli strai DH5$\alpha$. Three clones were identified that hybridized to mutiple restriction fragments of some formae speciales of F. oxysporum. These probes detected repetitive sequences in HindIII or EcoRI digested DNAs. Repeated copy clone pFC46, pFC52 and pFC54 showed evident polymorphisms among ten formae speciales of this fungus. Since clone pFC 52 strongly hybridized to multiple EcoRI-digested restriction fragments of f. sp. cubense, it may be useful as a probe for analysis of other genetic characteristics of this forma specialis. The results suggest that our clones might be very useful as probes for relatedness analysis between or within formae speciales of Fusarium oxysporum.

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Optical Card 시스템에서의 마이크로렌즈 조사 광프로브 어레이 설계 및 제작 (Design and Fabrication of Microlens Illuminated Aperture Array for Optical ROM Card System)

  • 강신일;김석민;김홍민;이지승;임지석
    • 정보저장시스템학회논문집
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    • 제2권1호
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    • pp.1-6
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    • 2006
  • An optical ROM card system which using an optical probe array generated by Talbot effect was proposed as new robust storage solution. To improve the optical density and to decrease the power consumption of the system, it is very important to make the spot sizes of optical probes smaller as well as to increase the optical efficiency from the light source to optical probes. In this study, a microlens illuminated aperture array for generating high efficiency optical probe away with small beam spot was designed and fabricated using monolithic lithography integration method. The maximum intensity of optical probes of microlens illuminated aperture array increased about 12 times of that of aperture array, and the full width half maximum of the optical probe at Talbot plane generated by microlens illuminated aperture array was $0.77{\mu}m$.

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