• 한국식품영양학회지
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• 제28권1호
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• pp.40-46
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• 2015

Hamcho (Salicornia herbacea) extracts were evaluated for total polyphenol content, antioxidant and pro-oxidant activities. The total polyphenol content was 1.81 g and 0.72 g per 100 g of dried sample in water and ethanol extracts respectively. Both water and ethanol extracts of Hamcho significantly exhibited antioxidant activity. The scavenging activity of hydroxyl radical was 13.8~26.2% and 14.2~16.0% in water and ethanol extracts respectively. The inhibitory effect of conjugated diene formation was 24.6~39.1% and 28.4~39.6% in water and ethanol extracts respectively. However, pro-oxidative effect was also observed in the Hamcho extracts. The Hamcho water extract showed the pro-oxidant effect by enhancing the formation of hydroxyl radical and conjugated diene. The ethanol extract of Hamcho induced conjugated diene formation at 0.5 mg/mL but not at 1 mg/mL. The hydroxyl radical formation was not induced by the Hamcho ethanol extract. Taken together, these results show that Hamcho extracts can act as pro-oxidants by generating hydroxyl radical or conjugated diene in addition to their antioxidant properties. Therefore, this study suggests that the physiological properties of Hamcho and its use as food materials should be considered with caution because antioxidant dietary plants such as Hamcho possess possible adverse effects induced by pro-oxidant activity.

• Nutrition Research and Practice
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• 제2권3호
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• pp.143-151
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• 2008

Many herbs have been used as therapeutics in Korean traditional medicine. In view of their clinical indications, anti-oxidant activity may contribute to their pharmacological effects. However, anti-oxidant information on these plants has not been available. In this study, seventy herbs which have been used in Korean traditional medicine were selected and screened for anti-oxidant activity using their water extracts. The anti-oxidant activity was assessed by their ability to inhibit three oxidation reactions; luminol/Fenton reagent, 2, 7-dichlorodihydrofluorescein(DCHF)/Fenton reagent and DCHF/peroxynitrite. In each assay, 70 herbs were divided into two groups; anti-oxidant group which inhibited the respective oxidation reaction and was majority(about 60 herbs), and pro-oxidant group which enhanced the oxidation reaction but was minority(more or less 10 herbs). When the herbs were listed in the order of their anti-oxidant strength, the orders obtained from each assay were found to be quite similar. The upper top rankers(more or less 10 herbs) in each assay showed strong activity compared to the others. The uppermost rankers in each assay were Rubus coreanus Miquel/Rubus schizostylus(覆盆子), Schisandra chinensis Baillon/Schizandra chinensis(五味子) and Terminalia chebula Retziusl Terminalia chebula(訶子). Of the pro-oxidant herbs, about 4-5 herbs were strongly pro-oxidant, which enhanced the control oxidation reactions to 150-300%. But the meaning of this observation is not known since few of them in one assay were also anti-oxidant in other assays. The results obtained in the present study may serve as information for understanding pharmacological effects of these herbs and developing new drugs from them.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.141.3-142
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• 2003

The inverse relationship between dietary flavonoids consumption and cardiovascular diseases may be associated with the ability of flavonoids to attenuate LDL oxidation. Although flavonoids have been employed to prevent against LDL oxidation, their pro-oxidant effect also deserves an attention in respect to untoward property. (omitted)

• 미생물학회지
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• 제50권1호
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• pp.22-26
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• 2014

Protocatechualdehyde (PA)는 항산화 활성과 항암 활성을 가진 페놀성 물질이다. Streptomyces lincolnensis M-20 균주에서 생산된 PA를 균주 상등액에서 분리, 정제하였다. 항산화 활성을 가진 PA가 구리 이온 존재 하에서는 산화촉진제로 작용하였다. 항산화 활성은 DPPH를 이용한 방법으로 측정하였으며, 구리 이온 존재 하에서 PA의 산화 촉진 작용은 pBR322 플라스미드의 DNA 절단 작용으로 측정하였다. DNA 손상으로 생성되는 활성산소 종의 확인은 활성 산소종의 포집자인 글루타치온에 의해 DNA 절단이 억제되는 것으로 확인하였다. PA와 구리 이온의 복합체 형성은 금속 이온의 킬레이트인 EDTA가 존재할 경우와 존재하지 않을 경우를 자외선/가시광선 분광학적 분석법으로 비교, 확인하였다.

• 한국포장학회지
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• 제28권3호
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• pp.165-170
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• 2022

최근 지구온난화 심화와 환경재난 등으로 석유유래 플라스틱의 분해에 대한 관심이 높아지고 있다. 플라스틱의 분해를 촉진하는 산화촉진제로 ferric ion(Fe²⁺)을 농도별로 첨가하여 필름을 제조하였다. 대조군으로 LLDPE필름과 산화촉진제를 농도별로 첨가한 필름에 UV를 시간별로 조사하여 인장강도, 신장율과 분자량변화를 조사하였다. 수지에 ferric ion 첨가량이 많아질수록 물성저하가 컸으며, 분자량 변화도 큰 것으로 나타났다. 인장강도는 대조군에 비해 산화촉진제 첨가필름이 조사시간 100시간 후 부터는 현저하게 저하되었으며, 이러한 현상은 신장율에서도 비슷한 것으로 나타났다. 분자량은 산화촉진제 첨가에 따른 결과로 UV 조사 50시간 후 63.6%, 100시간 후에는 73.8% 분자량이 감소한 것으로 나타났다. 이상의 결과에서 플라스틱 필름에 산화촉진제인 ferric ion(Fe²⁺)을 첨가함에 따라서 필름의 강도와 분자량이 저하되는 결과와 김 등 등의 보고를 바탕으로 필름의 분자량이 저하되면 그만큼 필름이 분해되고 있다고 판단할 수 있을 것으로 사료되었다.

• Environmental Analysis Health and Toxicology
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• 제30권
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• pp.6.1-6.7
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• 2015

Objectives This study aimed to estimate the effects of 4-nonylphenol (NP), a ubiquitously present surfactant in aquatic environments, on the anti-oxidant systems of the liver in the Far Eastern catfish Silurus asotus. Methods Changes in biochemical parameters involved in glutathione (GSH)-related and other anti-oxidant systems were analyzed following 4 weeks of 4-NP administration (0.1 and 1.0 mg/kg diet) via a formulated diet to catfish. Results 4-NP exposure induced an elevation in hepatic lipid peroxide levels and an accompanying decrease in reduced state GSH after 2 weeks, suggesting pro-oxidant effects of the chemical in catfish. This oxidative stress was associated with an inhibition of the GSH-utilizing enzyme glutathione peroxidase at the same time point. This inhibition was restored after 4 weeks. The activities of other anti-oxidant enzymes, i.e., glutathione reductase, superoxide dismutase and catalase were increased after 4 weeks. These enzyme increases occurred more strongly at the higher 4-NP concentration (1.0 mg/kg diet). Conclusions 4-NP given to catfish at 0.1 to 1.0 mg/kg diet, concentrations relevant to environmental levels, depletes the endogenous anti-oxidant molecule GSH and temporarily inhibits GSH-related anti-oxidant enzymes. Such declines in anti-oxidant capacity and elevated oxidative stress seem to be compensated eventually by subsequent activation of various anti-oxidant enzyme systems.

• 약학회지
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• 제48권2호
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• pp.111-116
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• 2004

The ethanol extracts of mixed vegetables (Bioactive Vegetables, BV), mixed fruits (Bioactive Fruits, BF) and their liquid formulation (Chungpae Plus , CP) were evaluated for their antioxidative and antigenotoxic activities. They were shown to possess the significant free radical scavenging effect against 1,1-diphenyl-2-picryl hydrazine (DPPH) radical generation and were revealed to show the inhibitory effect of lipid peroxidation as measured by malondialdehyde (MDA) formation although the potencies were not higher than those of vitamin C. They did not possess any pro-oxidant effect on bleomycin-Fe(III) dependent DNA degradation, whereas vitamin C showed strong pro-oxidant effect. Furthermore, oral administration of BV and BF inhibited micronucleated reticulocyte (MNRET) formation of mouse peripheral blood induced by KBrO3 treatment in vivo. CP also showed significant inhibition under same experimental condition. Therefore, the liquid formulation (CP) containing BV and BF may be a useful natural antioxidative and antigenotoxic agent by scavenging free radicals, inhibition of lipid peroxidation and protecting chromosomal damage.

• 한국식품영양과학회지
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• 제46권1호
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• pp.18-25
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• 2017

본 연구는 삼채의 조추출물과 유기용매 분획물들을 가지고 DPPH 라디칼 소거 활성에 의한 항산화 활성 검색 결과 디클로로메탄($CH_2Cl_2$) 분획물과 에틸아세테이트(EtOAc) 분획물에서 라디칼 소거 활성을 나타냈으며, xanthine oxidase 억제 효과는 DPPH 활성 라디칼 소거 활성에서 제일 뛰어났던 에틸아세테이트 분획물에서, superoxide 소거 활성은 헥산(n-hexane) 분획물에서 활성이 나타났다. RAW 264.7 세포에 lipopolysaccharide로 자극을 주고 삼채 주정 추출물 및 유기용매 분획물들을 처리하여 확인해본 결과, 조추출물과 물 분획물을 제외한 나머지 유기용매 분획물에서 염증유발 인자(NO, $PGE_2$, iNOS, COX-2, IL-6 및 $IL-1{\beta}$) 생성억제 효과가 나타났으며, 그중 디클로로메탄 분획물과 에틸아세테이트 분획물에서 억제 효과를 확인할 수 있었다. 본 실험 결과 삼채 조추출물과 유기용매 분획물에서 항산화 효과 및 염증 유발 인자의 생성 억제 효과가 나타났으며, 이러한 결과 삼채에서 유효성분 추출을 통한 항산화, 항염증 물질의 연구 또는 예방하거나 치료할 수 있는 염증 억제 성분의 분리 및 그 작용기전 연구에 중요한 기초 자료가 될 것이라 생각한다.

• 한방안이비인후피부과학회지
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• 제33권2호
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• pp.100-111
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• 2020

Objectives : The aim of experiment is to examine anti-inflammatory effect and anti-oxidant effect of Naesohwangryun-tang (NSHRT) in LPS-stimulated RAW264.7 macrophage cells. Methods : In the present study, The cell viability was performed by MTT assay. Nitric oxide (NO) production and prostaglandin E₂ (PGE₂) synthesis were performed by NO assay and ELISA KIT. The anti-oxidant effect was performed by DPPH and ABTS radical scavenging activity. The inhibitory effects of pro-inflammatory mediators and cytokines were confirmed by realtime PCR and western blotting. Results : NSHRT was no cytotoxicity at treated group. NO and PGE2 production were inhibited compared to the LPS treated group and also mRNA and protein expressions were significantly decreased compared to the LPS treated group. Conclusions : According to the above experiments, we confirmed that NSHRT has anti-inflammatory and anti-oxidant effects. It is suggested that NSHRT is potential ingredient of skin diseases.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book I
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• pp.313-319
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• 2003

It is well known that ascorbic acid (VC) is an important factor for several physiological reactions. In the skin, VC works as an anti-aging agent due to removing of oxidative stress generated by UV irradiation and stimulation of collagen synthesis. Thus, developing more effective VC derivatives is an important issue in creating anti-aging skin care products. Our study succeeded to develop a novel ascorbic acid derivative, ascorbic acid tetra-2-hexyldecanoate (VC-IP), which is a lipid-soluble pro-VC. The purpose of this study was to indicate the effects of VC-IP as pro-VC and anti-aging agent. First, it was examined whether VC-IP is converted to VC in physiological conditions. Since VC was detected from the cell extracts treated with VC-IP, it was indicated that VC-IP is a pro- VC.(omiited)