• Asian Pacific Journal of Cancer Prevention
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• v.13 no.3
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• pp.915-921
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• 2012

Background: Previous studies investigating the association between TP53 Arg72Pro polymorphism and gastric cancer (GC) risk in Asian population have reported controversial results. Thus, a meta-analysis was performed. Methods: A comprehensive literature search was conducted and 17 case-control studies were finally included, involving a total of 5,990 GC cases and 6,812 controls. Subgroup analyses were performed by the sample size. Results: Meta-analysis of all 17 studies showed variant genotypes of TP53 Arg72Pro to be associated with an elevated GC risk in three genetic comparison models ($OR_{Pro\;vs.\;Arg}$=1.13, 95%CI 1.03-1.25, $P_{OR}$=0.01; $OR_{Homozygote\;comparison\;model}$=1.33, 95%CI 1.07-1.64, $P_{OR}$=0.009; $OR_{Dominant\;genetic\;model}$=1.13, 95%CI 1.05-1.22, $P_{OR}$=0.002). Besides, a more obvious association was observed after the heterogeneity was decreased (all P values less than 0.001). This association was further identified by both subgroup and sensitivity analyses. Conclusions: This meta-analysis suggests the Pro variant of TP53 Arg72Pro contributes to gastric cancer risk in Asians.

• Journal of the Korean Home Economics Association
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• v.41 no.3
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• pp.93-111
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• 2003

This study aimed to examine the water consumption of urban households according to pro-environmental attitude for environmental protection. In contrast to preview studies, this study purposed to include various related independent variables, motive to environmental behavior, in special, in the model, and suggest informative data for research, education and strategies related to environmental protection. The data were from 665 housewives living in five urban areas. For the analysis of data, frequencies, means, percentages, GLM analysis, DMR test and Chi-square test were used. The main results of this study were as follows; 1. The respondents held high pro-environmental attitude that pro-environmental behaviors are important to protect environment. The pro-environmental attitude among the respondents were statistically different from the independent variables : spouse's occupation, living area, help of housekeeper, knowledge about environmental protection, convenience to check water consumption, and perception of voluntary conservative behavior among neighborhood 2. There were great difference on water consumption among respondents. The significant independent variables to have effects on water consumption were different between water consumption per person and higher/lower average water consumption. The relationships of pro-environmental attitude and motive to pro-environmental behavior with two water consumption as dependent variables were unique. Also, living areas and knowledge about environment protection were consistently important to explain the difference of water consumption.

• Restorative Dentistry and Endodontics
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• v.26 no.3
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• pp.200-205
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• 2001

이 연구의 목적은 만곡 근관에서 다양한 크기로 근첨을 형성하였을 때 형성 전후의 근관의 전이 정도를 분석하고자 하는 것이다. 만곡된 근관을 가진 투명한 레진블럭 상에서 ProFile .06 taper와 K-flexofile을 이용하여 각각 근관형성을 시행하고 이를 똑같은 위치가 재현가능한 고정틀에 위치시킨 후 사진의 이중노출 기법을 이용하여 근관형성 전후의 근관의 전이정도를 분석한 결과 다음과 같은 결과를 얻었다. 1. ProFile의 경우 형성된 근관이 taper한데 비해 K-flexofiles의 경우 형성된 근관이 taper하지 못하고 확대가 더 컸다. K-flexofiles의 경우 ProFile의 경우보다 근첨 0~6mm 부위에서 유의하게 더 굵었다(p<0.05). 2. 만곡외측으로의 확대양상은 ProFile의 경우 대체적으로 taper하나 근첨 3~4mm부위가 약간 굵었다. K-flexofile의 경우 만곡외측으로의 확대양상은 taper하지 못하고 불규칙하였다. ProFile에 비해 근첨 0~2mm에서 유의하게 확대가 더 컸다(p<0.05). 3. 만곡내측으로의 확대양상은 모든 군에서 taper하였으나 K-flexofile의 경우 ProFile의 경우보다 확대가 더 컸다. K-flexofile의 경우 ProFile에 비해 근첨 3~5mm부위에서 유의하게 확대가 더 컸다(p<0.05).

• Journal of Veterinary Clinics
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• v.38 no.5
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• pp.215-220
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• 2021

The purpose of this study was to investigate the correlation between vertebral left atrial size (VLAS) and NT-proBNP levels. Sixty-three dogs with myxomatous mitral valve disease (MMVD) were recruited for this study. The MMVD group was classified according to the ACVIM stage based on medical history, clinical signs, and thoracic radiography and echocardiography findings. The N-terminal pro-B-type natriuretic peptide (NT-proBNP) levels were measured using fluorescent immunoassay. VLAS and VHS values were measured using a digital caliper in the right lateral view. There were significant differences in VLAS for each American college of veterinary internal medicine (ACVIM) stage (p < 0.01). As the ACVIM stage increased, it tended to increase. Vertebral heart score (VHS) also showed a tendency to increase with the ACVIM stage (p < 0.01). VLAS was divided into three groups: VLAS <2.6, 2.6≤ VLAS <3.1, and VLAS ≥3.1. NT-proBNP values were significantly different between the three groups (p < 0.01), and VLAS and NT-proBNP values showed a strong positive correlation (r = 0.756, p < 0.01). In conclusion, there were significant differences among ACVIM stages B1 and B2, B1 and C-D, and B2 and C-D groups regarding VLAS. Furthermore, NT-proBNP increased proportionally as VLAS increased. Measurements of VLAS may be helpful in the diagnosis of MMVD in addition to the existing radiological and echocardiographic methods.

• BMB Reports
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• v.55 no.3
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• pp.154-159
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• 2022

Protein S-glutathionylation is a reversible post-translational modification on cysteine residues forming a mixed disulfide with glutathione. S-glutathionylation, not only protects proteins from oxidation but also regulates the functions of proteins involved in various cellular signaling pathways. In this study, we developed a method for the detection of S-glutathionylated proteins (ProSSG) using eosin-glutathione (E-GSH) and mouse glutaredoxin 1 (mGrx1). ProSSG was efficiently and specifically labeled with E-GSH to form ProSSG-E via thiol-disulfide exchange. ProSSG-E was readily luminescent allowing the detection of ProSSG with semi-quantitative determination. In addition, a deglutathionylation enzyme mGrx1 specifically released E-GSH from ProSSG-E, which increased fluorescence allowing a sensitive determination of ProSSG levels. Application of the method to the adipocyte differentiation of 3T3-L1 cells showed specific detection of ProSSG and its increase upon differentiation induction, which was consistent with the result obtained by conventional immunoblot analysis, but with greater specificity and sensitivity.

• Journal of Families and Better Life
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• v.25 no.2 s.86
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• pp.105-122
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• 2007

The purpose of this study is to examine the influence of the family health on the self-esteem and pro-social behaviour of children. The objects were 422 children who were 5th and 6th grade in primary school. The questionnaires were collected between June and July in 2006. T-test, Pearson's correlation and hierarchical multiple regression were used for data analysis. The results are as follows: First, the family health is higher than the average and also the self-esteem and pro-social behaviour of children are higher than the average. Second, the higher family's economic and parent's educational level, the higher family health and self-esteem of children. In addition, Female children have higher pro-social behaviour than male children. Third, family health has positive correlations with self-esteem and pro-social behaviour of children. Furthermore there is a positive correlation between the self-esteem and pro-social behaviour of children. Fourth, the self-esteem and pro-social behaviour of children are strongly influenced by the family health. As a consequence the family health is the principal variable which influences on the self-esteem and pro-social behaviour of children. This study shows that the healthy family support program is necessary in order to build up the positive self-esteem and pro-social behaviour of children.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.1
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• pp.131-139
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• 2014

This study was performed to determine the effect of dietary supplementation of procyanidin on growth performance, blood characteristics, and immune function in growing pigs. In experiment 1 (Exp. 1), thirty-two crossbred pigs with an initial BW of $19.2{\pm}0.3$ kg were allocated into 4 treatments for an 8-wk experiment: i) CON (basal diet), ii) MOS 0.1 (basal diet+0.1% mannanoligosaccharide), iii) Pro-1 (basal diet+0.01% procyanidin), and iv) Pro-2 (basal diet+0.02% procyanidin). Pigs fed Pro-1 and Pro-2 diets had greater (p<0.05) gain:feed ratio compared with those fed CON or MOS 0.1 diets. Serum creatinine concentration was less (p<0.05) in Pro-2 treatment than those in CON, MOS 0.1 and Pro-1 treatments. In Exp. 2, twelve pigs (BW $13.4{\pm}1.3$ kg) received basal diet with i) 0 (CON), ii) 0.02% (Pro-0.02%), and iii) 0.04% procyanidin (Pro-0.04%) for 4 wk. Concentration of platelets was lower (p<0.05) in the Pro-0.04% group compared to CON at 24 h after lipopolysaccharide (LPS) challenge. In addition, secretion of cytokines from cultured peripheral blood mononuclear cells (PBMC) in the presence or absence of procyanidin was examined. The levels of interleukin (IL)-$1{\beta}$, IL-6 and tumor necrosis factor (TNF)-${\alpha}$ were lower (p<0.05) in Pro (LPS-stimulated PBMCs+procyanidin) than those in CON (LPS-stimulated PBMCs+PBS) at 4 h after LPS challenge. These data suggest that dietary addition of procyanidin improves feed efficiency and anti-inflammatory cytokines of pigs.

• International Journal of Industrial Entomology and Biomaterials
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• v.6 no.1
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• pp.81-85
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• 2003

To verify importance of the intervening sequence between the ribosome binding site (RBS) and the initiation codon for expression of Bacillus thuringiensis $\delta$-endotoxin, the pProMu, containing SphI and NcoIsites between RBS and the initiation codon of the cry1Ac gene, and the deletion derivatives of pProMu were constructed and transformed into the B. thuringiensis subsp. kurstaki $Cry^{-B}$ strain. The pProMu-ΔSphIhad identical six bases of intervening sequence to pProAc though the arrangement of sequence was different. Other mutants containing pProMu had 1 or 10 or 14 bases between RBS and the initiation codon. Among deletion mutants, only ProMu-ΔSphI/CB only produced 130 kDa typical bipyramidal crystals like those seen for ProAc/CB. However, ProMu/CB, $ProMu-{\Delta}NcoI$, and ProMu-ΔSphI＋NcoIdid not produce Cry1Ac crystals. In conclusion, the results suggest that 6-base intervening sequence was important for expression of cry1-type class gene. Furthermore, spacing effect of the intervening sequences may play an important role in expression of individual crystal proteins in B. thuringiensis without doubt.

• Journal of Nutrition and Health
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• v.38 no.9
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• pp.750-755
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• 2005

Hepcidin, a peptide hormone synthesized mainly by the liver, has been implicated as a key regulator of iron homeostasis. Results from studies with experimental animal models suggested that hepcidin levels are related with body iron status, but little data is available in human subjects. This study was conducted to determine the relationship between serum pro-hepcidin levels, blood indexes of anemia, and dietary iron intake in female college students. Serum pro-hepcidin concentrations were measured by enzyme-linked immunosorbent assay in eighty-two women with $22.1\pm0.2$ years old. Dietary intake data were collected by using the 24-hour recall method for 3 days. Mean concentrations of serum pro-hepcidin were 85.1 ng/ml$\pm$6.1(s.d.) with the range of 13.6-295.7 ng/ml. The median value of serum pro-hepcidin in the study subjects was 70.3 ng/ml. Serum pro-hepcidin concentrations were positively correlated with hemoglobin concentrations (r=0.273, p=0.013), and also with hematocrit (r=0.291, p=0.008). To examine whether the level of dietary iron intake affects serum pro-hepcidin levels, study subjects were divided into two groups according to the amounts of daily iron intake. Serum pro-hepcidin concentrations were $22\%$ lower in groups with low iron intake (${\leq}10.1$ mg/day), compared to high-iron intake group (>10.1 mg/day) . In conclusion, these data, as in agreement with findings in mice, suggest that hepcidin plays an important role in regulating iron metabolism in the human body.

• The Korean Journal of Pharmacology
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• v.29 no.2
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• pp.237-244
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• 1993

The effect of nicotine on the secretion of $[Met^{5}]-enkephalin$ (ME) in addition to proenkephalin A (proENK) mRNA levels and effects of indomethacin, nordihydroguaiaretic acid (NDGA), and captopril on nicotine-induced responses were studied in bovine adrenal medullary chromaffrin (BAMC) cells. Long-term exposure of BAMC cells to nicotine at a concentration of $10{\mu}M$ significantly increased proENK mRNA level and the secretion of ME into the medium. Treatment of BAMC cells with NDGA (a lipoxygenase inhibitor, $10{\mu}M$), indomethacin (a cycloooxygenase inhibitor) or captopril (an angiotensin converting enzyme inhibitor) alone did not affect ME secretion and proENK mRNA levels. The pretreatment of BAMC cells with NDGA inhibited the increased ME secretion and proENK mRNA level induced by nicotine. However, indomethacin and captopril did not affect nicotine-induced responses. Our results indicate that neuronal regulations of ME secretion and proENK mRNA level induced by nicotine in BAMC cells are in part mediated by a lipoxygenase-but not cyclooxygenase-and endogenous renin-angiotensin pathway.