• The Korean Journal of Mycology
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• v.18 no.4
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• pp.198-202
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• 1990

Some factors effecting on the mycelial growth and primordia formation of Agrocybe cylindracea were investigated. Among supplements added into sawdust, wheat bran was the best for the mycelial growth, and its appropriate concentration was 30%. A variety of sawdust preparations was tested singly and in combinations. Oak sawdust and the combination of oak and larch sawdust were the most effective for the mycelial growth. The optimal moisture content was 65%, and the range of optimal temperature was $25\:{\sim}\:28^{\circ}C$. The favorable conditions for the primordia formation appeared to be similar to those of the mycelial growth. Among supplements added into sawdust, wheat bran 30% and the combination of wheat bran and rice bran 30% were the best for the primordia formation. Oak sawdust, and the oak plus larch sawdust and oak plus poplar sawdust were the best for the primordia formation. The optimal temperature for the primordia formation was $17{\pm}2^{\circ}C$.

• Horticultural Science & Technology
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• v.16 no.4
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• pp.525-527
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• 1998

This study was conducted to determine the optimum cultural condition and method for in vitro mass production of Hibiscus syriacus L. 'Honghwarang'. When callus induced in MS solid medium supplemented with 0.01 mg/L TDZ was cultured in liquid medium containing 0.01mg/L TDZ, callus growth and shoot primordia formation was most effective. Formed shoot primordia were regenerated into shoot in MS or 1/2 MS medium of growth regulator-free condition. Effects of mesh size, shaking speed on callus and shoot primordia formation were examined after 5 weeks. Callus and shoot primordia formation was formed most effectively at 10 mesh and 80 rpm shaking speed in liquid medium.

• Geosciences Journal
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• v.23 no.4
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• pp.547-556
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• 2019

A new species of the most primitive rosenellid stromatoporoid Cystostroma, C. primordia sp. nov. is reported from the Hunghuayuan Formation (Lower Ordovician, Floian) of Guizhou Province in the South China Block and the Duwibong Formation (Middle Ordovician, Darriwilian) of the Taebaeksan Basin in mid-eastern Korea (North China Block). This species is the first representative of the genus found in both the North and South China blocks. Cystostroma primordia sp. nov. is characterized by the absence of denticles and distinctively smaller cyst plates (height 0.04-0.20 mm, length 0.09-0.39 mm) than any other known species of Cystostroma. The presence of C. primordia sp. nov. in Lower to Middle Ordovician strata of western Gondwana challenges the long-held view of the late Middle Ordovician emergence of Paleozoic stromatoporoids. The simple internal morphological features of this new species and its occurrence in the Lower Ordovician of South China strongly indicate that an Early Ordovician Cystostroma-type precursor from western Gondwana is located near the base of the stromatoporoid stock. This occurrence greatly preceded the late Middle Ordovician (late Darriwilian) stromatoporoid diversification in circum-equatorial regions worldwide.

• Korean journal of applied entomology
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• v.15 no.3 s.28
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• pp.153-159
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• 1976

Present paper was attempted to investigate the effects of the light on the sclerotium formation of some isolates of Sclerotinia sclerotiorum(Lib.) do Bary from lettuce, cucumber and rape. The investigations were performed in the both aspects inducing sclerotial primordia from the mycelial colony and developing such primordia. The cultures were grown on potato-dextrose agar and were treated with day-light fluorescent lamps. In the continuous light illumination for 21 days, numbers of sclerotial primordia and matured sclerotia were increased with increasing the light intensity up to about 500 Lux, but on the contrary the dry-weight of matured sclerotia was decreased. In the 800 Lux illumination, induction of the primordia was extremely depressed, and then matured sclerotia were almost not produced. Short-time illumination for 48 hours, although the light intensity was as high as 5000 Lux, increased the numbers of sclerotial primordia and matured sclerotia. Dry-weight of matured sclerotia, however, was slightly increased only at low intensity of 160 Lux. On the other hand, the light shock which u·as alternatively on-of light every one minute for 48 hours increased the number and dry-weight of sclerotia produced, but the former was more effective at 500 Lux shock and the later more effective at 160 Lux shock.

• Journal of Mushroom
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• v.13 no.3
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• pp.223-227
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• 2015

The study was conducted to investigate the effects of post-incubation period and temperature treatment conditions during incubation on the uniform primordia formation and cultural characteristics of oyster mushroom (Pleurotus ostreatus). Three kinds post-incubation period; 25, 30, 35 days and control were applied for 30 days while two kinds incubation room temperature $23^{\circ}C$ and $26^{\circ}C$ and control were used $20^{\circ}C$. The substrate temperature during pre-incubation was of 'Suhan No. 1' and 'Gonji No. 7'. Oyster mushroom varieties tended to increase between $24^{\circ}C$ to $26^{\circ}C$ at 11 to 15 days after inoculation and then they were maintained in treatment temperature during post -incubation period. The $CO_2$ occurrence was at the highest at 6,500 ppm for 'Suhan No. 1' and 5,800 ppm for 'Gonji No. 7' at the time of the highest temperature increase. The ratio of un-uniformal primordia formation and the ratio of non-commercial fruit body were reduced by 40%, 10.5%, respectively compared to control for 'Suhan No. 1' when in the post-incubation temperature was $26^{\circ}C$, and incubated for 10 days and 15 days treatment. Also, 'Gonji No. 7' was reduced by 19%, 9.5%, respectively when in the post-incubation temperature was $26^{\circ}C$, and incubated for 10 days treatment. Therefore, the higher post-incubation temperature of room and longer post-incubation period resulted in the higher percentage of primordia formation of two cultivars.

• Korean Journal of Plant Resources
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• v.33 no.6
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• pp.682-688
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• 2020

Hemp (Cannabis sativa L.) is a dioecious plant, although monoecious plants are bred in some cultivars for fiber or seed production. Recently, hemp has received attention for medicinal use with some cannabinoids, including cannabidiol. Self-fertilization for breeding inbred lines is difficult because of dioeciousness and anemophily in hemp. This experiment was conducted to develop a self-fertilization method by forming female flowers and seeds from male plants of dioecious hemp. To induce the formation of female flowers on male plants, 500 mg L^-1 of ethephon was sprayed on plants at soon, seven and fourteen days after primordia formation. The plant ratio of female flowers formation and the number of harvested seeds were increased by ethephon treatment. Female flowers of male plants have 5 stigmas in contrast to the dual stigma of female 1plants. Male plant seeds were lighter and smaller than those from female plants. Although the germination rate was lower than that of normal seeds from female plants, the seeds from male plants germinated to grow seedlings. Thus, we suggest that hemp plants should be treated with ethephon at soon after primordia formation to induce the formation of more female flowers on the male plants.

• Mycobiology
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• v.32 no.4
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• pp.190-193
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• 2004

Six strains of Sparassis crispa such as S. crispa DUM-01, DUM-02, DUM-03, DUM-04, DUM-05, and DUM-06 were tested for their mycelial growth on 6 different kinds of sawdust media and primordial formation on 10 different compositions of larch sawdust media. The highest mycelial growth was recorded on the larch sawdust. Of the 6 strains of S. crispa, S. crispa DUM-04 recorded the favorable formation of primordia. The primordial formation of S. crispa DUM-04 was more favorable on L-3 medium than 9 kinds of larch sawdust media. When S. crispa DUM-04 was cultured on the media of larch sawdust+pine sawdust, the formation of its fruiting bodies was more outstanding on the media of larch sawdust+pine sawdust than those of larch sawdust.

• Horticultural Science & Technology
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• v.16 no.4
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• pp.520-524
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• 1998

This study was carried out to investigate the effect of thidiazuron(TDZ) on callus and shoot primordia formation, to determine the most optimum multiple shoot induction medium, and to obtain the plantlets on solid medium via shoot organogenesis. TDZ 0.01 mg/L in MS medium was most effective on callus formation, and BA 0.1 mg/L was most effective on shoot growth, while TDZ 0.01 mg/L was most effective on callus formation. TDZ 0.001 mg/L was most effective in shoot primordia formation. Shoot tips were cultured with TDZ 0.01 mg/L for 8 weeks and induced callus was transferred to regeneration medium containing TDZ 0.001 mg/L. After 4 weeks induced shoot primordia were resubcultured at growth regulator-free medium for 4 weeks. The induced multiple shoots rooted more efficiently at NAA 1.0, 5.0 mg/L, or IBA 5.0 mg/L.

• Journal of Plant Biology
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• v.33 no.4
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• pp.271-276
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• 1990

Our study was carried out for plant regeneration via somatic embryogenesis from immature seeds of Bletilla striata. The highest frequency of embryogenic callus formation was obtained from the immature seeds (at 150 days after pollination) cultured on Hyponex and VW medium supplemented with 3 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg/l kinetin under the dark condition. Multiple somatic embryos were induced when embryogenic callus was transferred to VW medium without growth regulators under continued illumination. Somatic embryos were observed histologically with scanning electron microscopy. Regeneration of Bletilla striata was obtained from somatic embryos with a well-defined scutellum and coleoptile as well as with one or more shoot primordia and root primordia. We think that these methods for orchid multiplication must be useful to access clonal propagation of orchids.

• Mycobiology
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• v.34 no.4
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• pp.206-208
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• 2006

To produce an artificial fruiting body of Armillaria mellea on the oak sawdust medium, seven strains of A. mellea were used. The top surface of oak sawdust medium covered with ground raw carrot was inoculated with each of 7 strains and cultured for 30 days at $25^{\circ}C$ in the dark condition until the mycelia of A. mellea completely colonized the medium from top to bottom. Then, the mycelia which were fully covered on the top surface of the medium were scratched slightly with a spatula and filled with tap water for 3 hours. To induce the primordial formation, the 7 strains of A. mellea were transferred to the growth chamber under the illumination (350 lux) of 12 hours and relative humidity of $85{\pm}5%$ in a day and then cultured at $16{\pm}1^{\circ}C$. Only A. mellea IUM 949 could form primordia on the sawdust medium, but the other strains did not make primordia at the same condition. The primordia of A. mellea IUM 949 were formed 10 days after complete colonization of the medium and the fruiting bodies were produced 7 days after a primordial formation. The experimental results suggested that IUM 949 strain might be a good candidate for mass production of fruiting bodies of A. mellea.