• The Journal of the Korea Contents Association
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• v.20 no.4
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• pp.582-590
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• 2020

This paper aims to analyze and introduce the primary English curriculum of North Korea reformulated according to the New Educational Program. Sources for analysis are the 4th and 5th primary school English syllabus based on the New Educational Program, explanations of the New Educational Program appeared in People's Education and Kim, Jeong-Il's selected writings. The analytical sources are classified into characteristics, objectives, contents, methods and evaluation. The findings are as follows: The primary English education aims to reach to the basis of middle school English by learning English alphabets and basic English expressions. 4th graders learn basic oral English such as pronunciation, stress and intonation for the first semester and learn English alphabets and their sounds for the second semester. 5th graders learn familiar topics in English and repeatedly practice the important components of English such as pronunciation, vocabulary and grammar. The method is to maintain students' interests in English and encourage students to use classroom English. Also, structural practice is an important part of the method. Evaluation is primarily process-oriented and must motivate students to excel in English rather than fail in English.

• Animal Bioscience
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• v.35 no.11
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• pp.1787-1799
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• 2022

Objective: Choline deficiency, one main trigger for nonalcoholic fatty liver disease (NAFLD), is closely related to lipid metabolism disorder. Previous study in a choline-deficient model has largely focused on gene expression rather than gene structure, especially sparse are studies regarding to alternative splicing (AS). In modern life science research, primary hepatocytes culture technology facilitates such studies, which can accurately imitate liver activity in vitro and show unique superiority. Whereas limitations to traditional hepatocytes culture technology exist in terms of efficiency and operability. This study pursued an optimization culture method for duck primary hepatocytes to explore AS in choline-deficient model. Methods: We performed an optimization culture method for duck primary hepatocytes with multi-step digestion procedure from Pekin duck embryos. Subsequently a NAFLD model was constructed with choline-free medium. RNA-seq and further analysis by rMATS were performed to identify AS events alterations in choline-deficency duck primary hepatocytes. Results: The results showed E13 (embryonic day 13) to E15 is suitable to obtain hepatocytes, and the viability reached over 95% by trypan blue exclusion assay. Primary hepatocyte retained their biological function as well identified by Periodic Acid-Schiff staining method and Glucose-6-phosphate dehydrogenase activity assay, respectively. Meanwhile, genes of alb and afp and specific protein of albumin were detected to verify cultured hepatocytes. Immunofluorescence was used to evaluate purity of hepatocytes, presenting up to 90%. On this base, choline-deficient model was constructed and displayed significantly increase of intracellular triglyceride and cholesterol as reported previously. Intriguingly, our data suggested that AS events in choline-deficient model were implicated in pivotal biological processes as an aberrant transcriptional regulator, of which 16 genes were involved in lipid metabolism and highly enriched in glycerophospholipid metabolism. Conclusion: An effective and rapid protocol for obtaining duck primary hepatocytes was established, by which our findings manifested choline deficiency could induce the accumulation of lipid and result in aberrant AS events in hepatocytes, providing a novel insight into various AS in the metabolism role of choline.

• Bulletin of the Korean Chemical Society
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• v.16 no.11
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• pp.1064-1067
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• 1995

A convenient method for the synthesis of N-substituted isoindolin-1-ones is disclosed in palladium(0)-catalyzed heterocyclization of o-bromobenzylbromide with carbon monoxide (CO) and primary amines in DMF at 100 ℃.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.42 no.6
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• pp.9-18
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• 2005

This paper proposes the color decomposition method for multi-primary display (MPD) using a 3-dimensional look-up-table (3D-LUT) in a linearized LAB space. The proposed method decomposes conventional three-primary colors into the multi-primary control values of a display device under constraints of tristimulus match. To reproduce images on the MPD, the color signals should be estimated from a device-independent color space, such as CIEXYZ and CIELAB. In this paper, the linearized LAB space is used due to its linearity and additivity in color conversion. The proposed method constructs the 3-D LUT, which contain gamut boundary information to calculate color signals of the MPD. For the image reproduction, standard RGB or CIEXYZ is transformed to the linearized LAB and then hue and chroma are computed to refer to the 3D-LUT. In the linearlized LAB space, the color signals of a gamut boundary point with the same lightness and hue of an input point are calculated. Also, color signals of a point on gray axis are calculated with the same lightness of an input. With gamut boundary points and input point, color signals of the input points are obtained with the chroma ratio divided by the chroma of the gamut boundary point. Specially, for the hue change, neighboring boundary points are employed. As a result the proposed method guarantees the continuity of color signals and computational efficiency, and requires less amount of memory.

• The Transactions of the Korean Institute of Electrical Engineers B
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• v.48 no.4
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• pp.145-151
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• 1999

This paper presents the characteristic analysis method of PMLSM based on the Space Harmonic Analysis method considering the effect of stator slots. The magnetic field for unslotted primary can be easily calculated by the conventional space harmonic analysis method and the resultant magnetic field including slot effect is obtained using the concept of the virtual Equivalent Magnetizing Current(EMC) on primary core. And the influence of space harmonics in airgap flux density and static thrust due to slots is evaluated. In this paper, the results of the analytical method are compared with not only the experimental ones but ones of FEM to verify the validity of the proposed method.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.2
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• pp.243-247
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• 2009

Primary charging roller is rotated with contacting surface of OPC drum and take charge OPC drum. Owing to this reason, primary charging roller is made by elasticity substance with electric conduction. Properties of charging and image is changed by class of coating, method of coating and environment. This study developed coating material and coating method to make print image of good quality.

• The Transactions of the Korean Institute of Electrical Engineers
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• v.40 no.3
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• pp.250-257
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• 1991

In the analysis of the electric machine by finite element method, the primary current has been selected as a driving source. But the voltage is constant and the primary current varies according to the load condition in the pracdtical system. Therefore, in this paper, magnetic flux distribution, primary current, input effective power, power factor, efficiency and propulsion force of S.L.I.M. were calculated by the finite element method cnsidering the voltage as a driving source. Because the driving characteristics could not be measured in the S.L.I.M., voltage-current curve, 3-phase current curve, and propulsion force were measured at the starting and they were compared with theoretical values.

• Proceedings of the KIEE Conference
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• 1989.07a
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• pp.31-33
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• 1989

In this paper, a short primary-short secondary single-sided LIM which is used as a driving source for automatic conveyor system, is selected as a analysis model. And with a method for analyzing eddy current distribution and thrust force which are produced according to the relative position of primary and secondary, FEM in which current vector potential is introduced is adopted. Also, analysis results are compared with experimental results, so propriety of this at study is proved.

• Animal cells and systems
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• v.15 no.1
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• pp.19-27
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• 2011

Hepatocytes, the major epithelial cells of the liver, maintain their morphology in culture dishes coated with extracellular matrix (ECM) components such as collagen and fibronectin or biodegradable polymers (e.g. chitosan, gelatin). In these coated dishes, survival of cells and maintaining of liver-specific functions may increase. The aim of this study was to determine a suitable, cost-effective and simple system for hepatocyte isolation and culture which may be useful for various applications such as in vitro toxicology studies, hepatocyte transplantation and bioartificial liver (BAL) systems. In order to obtain primary cultures, hepatocytes were isolated from liver by an enzymatic method and cultured on plates coated with collagen, chitosan or gelatin. Collagen, gelatin-sandwich and gelatin-cell mixture methods were also evaluated. Morphology and attachment of the cells were observed by inverted microscope and scanning electron microscope (SEM). An MTT assay was used to determine cell viability and mitochondrial activity.

• The Transactions of the Korean Institute of Electrical Engineers
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• v.39 no.5
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• pp.453-461
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• 1990

In this paper, a short primary-short secondary single-sided linear induction motor which is used as the driving source for an automatic conveyor system, is selected as an analysis model. The finite element method in which the current vector potential is introduced is adopted to analyze the eddy current distribution and thrust force which are produced according to the relative position of the primary and the secondary. Also, the analysis results are compared with experimental ones, to show the propriety of this model.