• Parasites, Hosts and Diseases
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• v.23 no.2
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• pp.293-299
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• 1985

This study is to verify the protective ability against experimental Naegleria meningoencephalitis by immunization with Naegleria fowleri in mice. Naegleria fewleri, strain 0359, and Naegleria gruberi, strain EGB, were used in this study, and cultured in CGVS medium akenically. Inbred BALB/C mice, weighing about 20g, were immunized by three intraperitoneal injection of $1{\times}10^6$ N. fowleri trophozoites at the interval of one week. This N. fowleri trophozoites antigen was fixed with 5% formaldehyde. N. fowleri trophozoites from culture were homogenized with soiicator at $4^{\circ}C$ as monitored by phase contrast microscopy, and their membrane and cell content preparations were made for the immunization of mice. Their inoculation dose in volume was equivalent to the $1{\times}10^6$ trophozoites in each injection for immunization. And N. gruberi trophosoites, whieh was fixed with 5% formaldehyde, were also used for immunisation. Mice were inoculated intranasally with $5{\times}10^4$ N. fowleri trophozoites in a 511 suspension under anesthesia by as intraperitoneal injection of about 1 mg secobarbiturate. Nervousness, rotation or sluggish behaviour were observed in the mice which were infected with N. fewleri. Necrotic lesion was demonstrated in the anterior portion of brain, especially in the olfactory lobe. The inflammatory cell infiltration with numerous H. fowleri trophozoites was noticed. This pathological changes were more extensive in the control than in the experimental groups. Mice were dead due to experimental primary amoebic meningoencephalitis that developed between 8 days and 23 days after inoculation. Mortality rate of the mice was low in the immunized experimental group. Mean survival time, which is the survival duration of mice from the infection to death, was prolonged significantly in the immunized mice except in the mice immunized with JV, fowleri membrane. Even in the mice immunized with N. gruberi, survival time was delayed. In summary, the effectiveness of immunization is demonstrated in terms of protective immunity against Naegleria meningoencephalitis in mice.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.4
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• pp.86-92
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• 2017

In this study, a running performance evaluation and roller rig test was conducted to evaluate the applicability of a composite bogie frame, which has the role of the primary suspension. The composite bogie frame was made of a GEP224 glass/epoxy prepreg. Vehicle dynamic analysis was carried out on the composite bogie with three different kinds of side beam thicknesses (50 mm, 80 mm, and 150 mm). From the results, the composite bogie with a side beam thickness of 80 mm satisfied all the dynamic design requirements. Although the composite bogie with the side beam thickness of 50mm also met the design requirements, its critical speed was just a 2% margin to the requirement. In contrast, the model of the side beam thickness of 150mm did not meet the ride comfort. In addition, a composite bogie frame with the side beam thickness of 80 mm was fabricated and installed on a complete bogie. Moreover, the roller rig test using the fully equipped bogie was performed to evaluate the critical speed. During the test, the lateral excitation was imposed on the wheelsets to realize the rail irregularity. There was no divergence of the lateral displacement of the wheelsets while increasing the speed. The measured critical speed was similar to the predicted result.

• Proceedings of the Korea Water Resources Association Conference
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• 2015.05a
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• pp.169-169
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• 2015

유사 입자의 크기는 유사의 특성 및 그에 따른 거동 변화에 중요한 영향을 미친다. Stokes 침강 속도 모형에서 유사의 침강 속도에 가장 많은 영향을 주는 인자는 유사의 크기인 것 또한 확인된다. 유사 입자의 크기가 약 $60{\mu}m$보다 작은 유사들은 알갱이 사이의 점착력을 무시할 수 없다. 이로 인해 유사들은 응집 현상을 겪으며 입자 본래의 크기보다 크기가 큰 플럭을 형성하는 점착성 유사로 분류된다. 응집 현상이란, 흐름 내 점착성을 띠는 일차입자(Primary Particle)가 응집과 파괴를 반복하며 플럭을 형성하는 현상을 뜻한다. 입자 간의 충돌을 통해 응집이 진행되며 난류 전단으로 인해 형성된 플럭의 파괴가 발생한다. 많은 연구에서 점착성 유사의 충돌을 야기하는 가장 지배적인 원리는 난류라 알려져 있다. 이러한 응집 현상으로 인하여 플럭의 크기와 밀도는 지속적으로 변화를 겪으며 비점착성 유사와 다른 특징들을 보인다. 흐름에 존재하는 유사의 이동은 이송-확산 방정식을 통해 표현된다. 이송-확산 방정식은 시간 변화에 따른 농도의 변화를 입자의 침강과 난류 및 유사 자체의 특징에 의한 확산으로 해석한다. 침강속도로 대변되는 이송과 달리, 확산은 난류흐름 내에서 유사가 확산되는 정도를 정량화하기 위한 인자가 요구된다. 난류에 의한 유사의 확산은 유사 자체 특성에 따른 물질 확산에 비하여 매우 큰 값을 가지며, 이를 확산 계수로 개념화 한다. 확산계수는 와점성계수와 Schmidt 수(${\sigma}_c$)의 비로 정의된다. ${\sigma}_c$는 난류의 점성과 난류로 인한 부유과정에 의해 유사가 확산되는 정도를 나타낸다. 이에 따라 ${\sigma}_c$의 변화가 유사의 부유 및 침강거동에 많은 영향을 미칠 것이라 판단되나, 국내외에서 수행된 연구 동향에서는 ${\sigma}_c$를 0.5부터 1.0 사이의 상수를 적용하여 수행되었다. 이에 본 연구에서는 ${\sigma}_c$의 크기에 따라 달라지는 유사의 부유 및 침강 변화에 의한 총 부유량을 살펴보고자 한다. 유사의 점착성을 고려할 수 있는 1DV 수치 모형을 이용하여 비점착성 유사와 점착성 유사를 대상으로 수치연구를 수행하며, 유사의 크기 및 ${\sigma}_c$의 변화에 따른 총 부유량 경향을 살펴본다. 그 결과, 점착성 유사는 ${\sigma}_c$의 증가에 따라서 유사의 총 부유량이 증가하는 현상이 나타난 반면 비점착성 유사는 ${\sigma}_c$의 증가에 따라 유사의 총 부유량이 감소하는 경향이 나타났다. 그러나 크기가 아주 작은 비점착성 유사를 대상으로 수치 연구를 수행한 결과, ??에 따른 총 부유량의 경향은 유사의 점착성에서 기인하는 것이 아닌 입자의 크기로부터 야기되는 특성이라는 결론이 도출되었다.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.20 no.2
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• pp.65-71
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• 1984

As a primary step in studying the relationship between the arrangement of netting and the section shape of the bag net in the four-seam trawling net, a series of experiments were performed with the simplified model of the bag net made of vinyl film. This model was suspended horizontally in circularly flowing water, with two pairs of suspending threads to four points of symetry at the border of the bag mouth in place of both wing nets. And then, the area distribution of each panel in the bag net was arranged by the portion of the length of the side panel, q to that of the lower or upper panel, p at the border of bag mouth. In the experiments, the section shape of the bag mouth photographed and the tensions on both pairs of suspending threads were measured with two loadcells in circularly flowing water. From the results that the section shape of the bag mouth in circularly flowing water is controlled by the area distribution of panels in the bag net, the author estimated an experimental equation. h/w=k sub(1) e super(k) 2 super(q/p)+C, where h is the central height of the bag mouth, and w is the lateral width of that.

• Korean Journal of Soil Science and Fertilizer
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• v.6 no.3
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• pp.141-152
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• 1973

The mineralogical studies of the eleven sub-soil samples derived from granite, granodiorite, diorite and arkose sandstone, taken from apple orchards in the province of Kyungsangbukdo, Korea are made to investigate the relationships between the mineral weathering, soil forming processes and mineralogical composition. The fine sand fraction (less than 0.2mm) and the clay fraction (less than 2 micron) are dispersed with the shaker after hydrogen peroxide treatment for the removal of organic matter, and separated from each suspension by gravity sedimentation. The fine sand are observed by mineral microscope and the clay are observed by X-ray diffraction patterns, differential thermal analysis curves and infrared spectrum. The outline of the results are as follows. 1. The primary minerals ; Quartz, changed-feldspar, plagioclase, alkali-feldspar are dominant in almost all samples, and some samples contain an appreciable amount of hornblende, biotite, muscovite and plant opal. There are also those samples which contain very small quantity of pyroxene group, tourmaline, epidote, cyanite, magnetite, volcanic glass and zircon. They are mainly derived from weathering products of granite, granodiorite, diorite, arkose or its mixtures. 2. All samples contain expanding or nonexpanding $14{\AA}$ minerals, illite and kaolin minerals, and some samples contain chlorite, cristobalite, gibbsite, and those primary minerals as quartz and feldspar, but the quantities vary according to the parent matrials. 3. Non-expanding $14{\AA}$ minerals may be dioctahadral vermiculite which sandwiches gibbsite layer or chlorite in between layer lattices. 4. As for clay minerals, montmorillonite was principal component in the samples derived from weathering products of arkose sandstone and tertiary. Minerals which are derived from weathering products of arkose have kaolin minerals and vermiculite as their principal component, and minerals derived from weathering products of acidic rock group are generally classified into two groups, the kaolin mineral group, and the kaolin minerals and vermiculite group.

• Radiation Oncology Journal
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• v.17 no.2
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• pp.158-165
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• 1999

Purpose : To investigate the percentage of colonies wi1h16or more cells distribution of human skin fibroblast according to in vitro aging, and to evaluate the relationship between percentage of colonies with 10 or more cells and in vivo donor age in human skin fibroblast culture. Material and Method : C1, C2, C3a, and C3b human skin fibroblast samples from three breast cancer patients were used as subjects. The C1, C2, and C3a donor were 44, 54, and 55 years old, respectively. C3a and C3b cells were isolated from the same person. Single cell suspension of skin fibroblasts was prepared with primary explant technique. One hundred cells are plated into 100m1 tissue culture flask and cultured for two weeks. The colony size was defined as colonies with 16 or more cells. The cultured cell was stained with crystal violet, and number of cells in each colony was determined with stereo microscope at $\times$10 magnification. Passage number of C1, C2, C3a and C3b skin fibroblast were 12th, 17th, and 14th, respectively. Results : Percentage of colonies with 16 or more cells of skin fibroblast samples decreased with increasing in vitro passage number. In contrast, cumulative population doublings of skin fibroblast sample increased with increasing in vitro passage number. Percentage of colonies with 16 or more cells also decreased with increasing population doublings in human skin fibroblast culture. There was strong correlation with percentage of colonies with 16 or more cells and population doublings En C3a skin fibroblast sampie. At the same point of population doublings, the percentage of colonies with 16 or more cells of the young C1 donor was higher level than the old C3a donor. Conclusion : The population doublings increased with increasing in vitro passage number but percentage of colonies with 16 or more cells decreased. The results of this study imply that percentage of colonies with 16 or more cell is useful as a indicator of in vitro human skin fibroblast aging and may estimate the in vivo donor age.

• Journal of dental hygiene science
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• v.14 no.3
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• pp.342-348
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• 2014

This study was carried out an elementary school located in B-myeon and K-myeon of Gimje. One school (test group) with a school oral health care office and three schools (control group) without school oral health care offices were selected as sample schools. The dental caries prevention effects were compared between third to sixth graders who received benefits of the school continued oral health management program of K health office in Gimje, and first and second graders who did not receive the benefits due to the suspension of the program. The decayed, missing, and filled (DMF) rate, that received the benefits of the program, the test group was 58.9% and the control group was 76.1%, showing significant difference (p<0.05). For the DMF teeth (DMFT) rate, the who received benefits from the program, the test group was 41.1% and the control group was 64.2%, showing significant difference (p<0.01). For the DMFT index, the third to sixth graders that received benefits of the program, the test group was 1.73 and the control group was 3.66 showing significant difference (p<0.001). For the decayed teeth (DT) index, it was 0.72 for the test group and 1.96 for the control group showing significant difference (p<0.001). For the filled teeth index, the test group was 0.63 for the test group and 0.99 for the control group showing significant difference (p<0.05). For the DT rate, the total test group was 57.23% and 64.16% for the control group. For who received benefits from the program, the DT rate was 54.81% for the test group and 60.98% for the control group. The effects of the student continued oral health management program carried out by the oral health office can be confirmed. It is judged that efforts for continuous maintenance and promotion will be necessary to improve the oral health of students.