• Title/Summary/Keyword: Primary Culture

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A Study on Accumulation of Ni in Salix alba and S. caprea by Hydroponic Culture in Ni Solution (수경재배에 의한 Salix alba와 S. caprea의 Ni축적에 관한 연구)

  • Lee, Chang-Heon
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.11 no.5
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    • pp.1-11
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    • 2008
  • This study was carried out to provide primary data set for cleaning of contamination site. By having hydroponic culture on Salix alba and S. caprea seedling treated with Ni solution, the result of Ni accumulation came out as followings : In a treatment of Ni to the Salix alba and S. caprea, Ni accumulation increased in its root, leaves, and stem, as Ni concentration became higher until $10.0{\mu}mol$. But in a $100.0{\mu}mol$ treatment, the seedlings died after 4 weeks. Ni accumulation in the Salix alba and S. caprea was the highest in its roots, second-highest in leaves, and the lowest in stems. In the case of $10.0{\mu}mol$ treatment of Ni solution, Ni accumulation in roots were above 500.0mg/L, and leaves were above 20.0mg/L. But it was lower than 13.0mg/L in stems. Ni accumulation in the plant increased more when nutrient solution containing Ni was weekly changed than just refilling the same amount of nutrient solution that evaporated Ni accumulation in Salix alba was higher than S. caprea when the nutrient solution had been refilled only.

Growth and Antioxidant Production of Bacillus polyfermenticus SCD in Whey Protein Concentrate (WPC)-based Medium (유청단백질농축물을 기본 배지로 한 Bacillus polyfermenticus SCD균의 생육과 항산화물질 생산)

  • Choi, Gooi-Hun;Lee, Jang-Hyun;Jo, Mi-Na;Yoon, Yo-Chang;Paik, Hyun-Dong
    • Food Science of Animal Resources
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    • v.28 no.1
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    • pp.105-108
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    • 2008
  • The cell growth and antioxidant activity of Bacillus polyfermenticus SCD were studied in tryptic soy broth (TSB) medium and whey protein concentrate (WPC)-based medium. Overall, higher lactose contents in WPC-35 medium (up to 2.0%), and longer culture times correlated with greater cell viability. In WPC-35 medium with 1.5% and 2.0% lactose, the cell growth of B. polyfermenticus SCD was similar to growth in TSB medium. The 1,1-diphenyl-2-picyrylhydrazyl (DPPH) radical scavenging activity of culture supernatant of B. polyfermenticus SCD in WPC-35 medium was measured to assess antioxidant activity. The antioxidant activity increased up to 32 hr of culture, reaching a maximum of 75.57% DPPH radical scavenging activity. The antioxidant activity seemed to follow the typical kinetics of primary metabolite synthesis. The antioxidant activity of B. polyfermenticus SCD supernatant in WPC-35 medium was more effective and stable than supernatant from TSB medium. These results suggest that WPC-35 medium is effective for the production of antioxidant by B. polyfermenticus SCD.

A Study on the Activation Plan of Smart Work in Public Sector (공공부문 스마트워크 활성화 방안에 관한 연구)

  • Sung, WookJoon
    • Informatization Policy
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    • v.20 no.3
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    • pp.43-62
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    • 2013
  • This study aims to derive primary policy challenges for the continuous diffusion of the smart work centers in the public sector by using the AHP survey. The policy challenges in the AHP survey were categorized into three aspects, namely technology, system, and awareness. The technology sector was divided into the improvement of smart work-related ICT and the facility improvement in a smart work center. The system sector was divided into the legal system and implementation system. The awareness sector was divided into organizational culture and individual awareness. Among total 12 policy challenges, the results of the AHP analysis showed that the improvement of the organizational culture was the policy issue of the highest priority in terms of importance and urgency.

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Principal protocols for the processing of cultured meat

  • Lee, Seung Yun;Kang, Hea Jin;Lee, Da Young;Kang, Ji Hyeop;Ramani, Sivasubramanian;Park, Sungkwon;Hur, Sun Jin
    • Journal of Animal Science and Technology
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    • v.63 no.4
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    • pp.673-680
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    • 2021
  • The purpose of this study was to establish a basic principal procedure for the processing of cultured meat. The first stage involved isolating satellite cells from the desired muscle of an animal using enzymatic digestion (i.e., by using proteases, collagenases, and pronases). The second stage involved culturing the isolated muscle satellite cells in a growth medium containing fetal bovine serum and penicillin/streptomycin with growth factors for an optimal period of time. The second stage involved a basic method for the isolated muscle cells to proliferate while sub-culturing to further induce differentiation in gelatin-coated culture dishes with the general culture medium. The third stage involved the induction of differentiation of muscle satellite cells or formation of myotubes using myogenic medium. Lastly, the fourth stage involved the identification of cell differentiation or myotube formation (myogenesis) using fluorescent dyes. Moreover, the principle of these protocols can be applied to perform primary culture of animal cells. This study will assist beginners with the technical aspects of culturing meat (isolation, cultivation, and differentiation of muscle satellite cells as well as identification of myotube formation for myogenesis).

Profile of Korean Restaurant Patrons in New-york City (뉴욕 소재 한국레스토랑 고객특성 분석)

  • Han, Kyung-Soo;Sung, Heidi H.
    • Journal of the Korean Society of Food Culture
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    • v.24 no.6
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    • pp.655-665
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    • 2009
  • Coupled with the international expansion of Korean culture in recent years, a number of restaurants from Korea have been trying to tap into the global market place. The purpose of this study was to identify the characteristics of non-Korean patrons in Korean Restaurants in New-york city. The survey was conducted at six popular Korean restaurants, all of which had been recognized in the Zagat Survey in recent years, located in prime business districts in Manhattan. The data collected from the six local Korean restaurants that participated in this study were qualitatively and quantitatively analyzed. After employing individual in-depth interviews with restaurant operators, a qualitative analysis identified demographic characteristics, Socioeconomic characteristics and segmentation of restaurant operation. Self-administrated survey questionnaires were used to acquire quantitative data. Primary data were collected from non-Korean patrons at the six participating Korean restaurants in New York City in 2008 (N=245). The patrons who answered the survey indicated that they were highly satisfied with the 'Food'; however, they were not satisfied with the 'Beverage' and 'Value'. In addition, older patrons (55<) were not as content with the 'Food' as the younger patrons. The most influential satisfaction variable that affected a patron's intention to revisit the Korean restaurant was 'Food' and 'Overall experience'. This study findings will help Korean restaurant operators and marketers better understand their patrons and formulate strategies to cater and target segments more effectively.

How to measure fashion stress? Development and validation of a multidimensional scale for fashion stress (패션 스트레스는 어떻게 측정할 수 있는가? 패션 스트레스의 다차원 척도 개발 및 타당화)

  • Hyojung Suk;Eun-Jin Lee
    • The Research Journal of the Costume Culture
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    • v.32 no.2
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    • pp.181-198
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    • 2024
  • Fashion stress is a pertinent aspect of modern consumer culture that has been underexplored in academic research. This study developed a conceptual framework of fashion stress and a multidimensional scale to measure consumers' fashion stress. The qualitative study included literature reviews on consumption stress, shopping stress, and consumer behavior, as well as focus group interviews to gain insight into various dimensions of fashion stress. NVivo 12.0 was used to analyze the qualitative data and identify core categories following the grounded theory methodology. The quantitative study involved a preliminary and a primary surveys to verify the validity and reliability of the fashion stress scale. A total of 220 questionnaires were used for data analysis. The results show that fashion stress consists of eight factors: care, shopping, fit, brand, financial, closet, style, and disposal. Choice difficulty plays a significant role in all factors of fashion stress. Moreover, shopping stress had a negative impact on impulse buying, while other factors such as fit, brand, closet, and disposal stress had a positive impact on impulse buying. Thus, fashion stress is a potential antecedent of impulsive consumer behavior. The results also confirm the validity and reliability of the scale. The fashion stress scale developed in this study offers researchers a valuable tool for assessing and understanding consumer experiences.

The Effects of Selenium on Cadmium-Induced Toxicity and Lipid Peroxidation in Rat Hepatocyte Primary Culture (랫드 간세포 일차배양에서 셀레늄이 카드뮴에 의해 유도된 독성 및 지질과산화에 미치는 영향)

  • Rhim, Tae-Jin
    • Korean Journal of Environmental Agriculture
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    • v.22 no.2
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    • pp.94-99
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    • 2003
  • The objective of present study was to investigate the antioxidative and hepatoprotective effects of selenium on cadmium-induced toxicity and lipid peroxidation in rat hepatocyte primary culture. To do this, two separate experiments were conducted. In Experiment 1, primary cultures of rat hepatocytes were incubated for 6 hr in the presence of various concentrations (1, 10, 50, 100, and $500\;{\mu}M$) of cadmium chloride. Cytotoxicity and lipid peroxidation were evaluated using the MTT assay and TBARS assay, respectively. Antioxidative and hepatoprotective effects were determined by measuring the activity of GOT and GSH-Px, respectively. Cell viability was reduced and lipid peroxidation was increased by cadmium in dose-dependent manners. There was significantly negative correlation (r=-0.943, p<0.01) between cell viability and lipid peroxidation GOT activity was increased and GSH-Px activity was decreased by cadmium at the concentration of $50\;{\mu}M$. In Experiment 2, primary cultures of rat hepatocytes were incubated for 6hr in the presence of 100\;{\mu}M$ of cadmium chloride and various concentrations (0.01, 0.1 and 1 ppm) of sodium selenite to assess the effect of selenium on cadmium-induced toxicity and lipid peroxidation. Cell viability and GSH-Px activity were increased by sodium selenite at the concentration of 1 ppm Whereas, lipid peroxidation and GOT activity were reduced by 0.1 ppm of sodium selenite. These results demonstrate that selenium has an antioxidative and hepatoprotective potentials against cadmium.

A survey research on the primary teachers' perception of social emotional learning in Malawi (말라위 초등교사의 사회정서학습에 대한 인식조사 연구)

  • Park, Hwanbo
    • Korean Journal of Comparative Education
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    • v.27 no.4
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    • pp.73-96
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    • 2017
  • This study aims to investigate the primary teachers' perception of social emotional learning (SEL) in Malawi. To achieve this purpose, total 78 questionnaires were gathered from 9 primary schools in rural area. The data was analyzed with independent-samples t-test, correlation analysis, and multiple regression analysis. The results of this study are follows. First, the sub-factors of the Malawian primary teachers' perception of SEL shows that Comfort is 4.10, Commitment is 4.59, and Culture is 3.78. Second, there was a significant difference in the Malawian primary teachers' perception by age, years of teaching, and participation to in-service training about SEL among the individual characteristics. Third, the Malawian primary teachers' degree of implementation of SEL in school was 4.01, and there was a significant difference by years of teaching. Forth, among the sub-factors of teachers' perception of SEL, Comfort had a positive effect on the degree of implementation of SEL. This result implied that the reason why Malawian primary teachers had difficulty in implementing SEL in the school might be not a lack of individual competence but feel uncomfortable with implementation. Therefore, in order to implement SEL in the Malawi school, it is necessary to support teachers so they have confidence and comfort psychologically.

A Study on Costume Culture Interchange Resulting from Political Factors (정치적 요인에 의한 복식문화교류에 관한 연구)

  • Yu Ju-Ri;Kim Jeong-Mee
    • Journal of the Korean Society of Clothing and Textiles
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    • v.30 no.3 s.151
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    • pp.458-469
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    • 2006
  • The purpose of this study was to prove that interchange is a primary factor in costume changes through case analysis in costume culture interchange, and further, to assist in gaining understanding of costume changes of the present and the future, and therefore, in this study, cases analysis was conducted focusing on interchange resulting from political factors. In order to present theories that are able to analyse costume culture interchange, culture, cultural exchange, and costume cultures were examined, and based on the results, a costume culture interchange process model and its three steps, propagation, selection, and reinterpretation, were presented. The results of case study on costume culture interchange resulting from political factors based on the costume culture interchange process model presented are as follows. Interchanges that politically uses the possibility of expressing costume as symbols are in most cases aggressive and semi aggressive. In order to exhibit superiority of themselves, the aggressors forced their national costumes onto the receiving culture. Rather than a simple introduction of their costumes, it was an attempt to introduce they values and their ways of life. The conclusion that can be reached through such result is that interchange is an important driving force for changes in costume culture. All interchanges were not greatly affected by methods of propagation and acceptance, and resulted in fusion. Fusion is a process in which existing costume and foreign costume come together constructively to for a new costume culture therefore change in costume style is inevitable.

Development of Fashion Design Depending on the Modern Woman's Contra-sexual Trend (현대 여성의 콘트라섹슈얼 성향에 따른 패션 디자인 개발)

  • Yang, Eun-Jin;Lee, In-Seong
    • The Research Journal of the Costume Culture
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    • v.17 no.5
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    • pp.733-745
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    • 2009
  • The contra-sexual phenomenon that is recently focused is one of the new factors, which is focused when explaining the life style of progressive woman's image and it is creating cultural category. Moreover, the contra sexual people that are rising as new consuming subjects are not simple primary consumers but their shopping culture and cultural tastes are giving effects to many people. Because of the tendency, marketer of consuming industry are making efforts to predict the changes of contra-sexual people's shopping habits and tastes. Therefore, it is necessary to grasp contra-sexual phenomenon and research the fashion design aiming at that in order to predict woman's fashion image in the future. Therefore, the study considered concept and characteristic of contra-sexual phenomenon and surveyed its example in the popular culture. And, the study analyzed the formative characteristic of contra-sexual fashion shown in the public cultures such as drama and movie. On the basis of the result, design was suggested by reflecting contra-sexual fashion characteristic under the concept of 'Urban Splendor'. From the results above, it was possible to grasp the concept and characteristic of contra-sexual phenomenon, which appeared as a trend and understand new woman's image and the fashion that has changed because of it. It is considered that the establishment of the foundation of contra-sexual phenomenon, which plays as an important variable in woman's fashion, fashion accessory, cosmetic and advertisement field but it is hard to clarify, by analyzing the work aiming at contra-sexual phenomenon will be helpful for predicting the change of woman's fashion in the future.

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