• Title/Summary/Keyword: Preservation condition

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Myocardial Preserving Effect of Fructose-1, 6-diphosphate in Isolated Rat Heart (적출된 쥐심장을 이용한 Fructose-1, 6 diphosphate의 심근보호 효과에 관한 실험)

  • 이철주;조수신;류재온;김문환;김세환;이익호;박소라;서창국
    • Journal of Chest Surgery
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    • v.26 no.2
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    • pp.75-79
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    • 1993
  • Myocardial protection against ischemic and reperfusion injuries is still in troublesome eventhough couples of the way of myocardial protection have been applied since 1970's. One of the possibility in myocardial protection is adding Fructose-l,6-diphosphate(FDP) in cardioplegic solution. It is assumed that FDP can promote ATP production under anaerobic condition as well as inhibiting the supressing effect of lactate on phosphofructokinase. We compared the myocardial protecting effects of FDP in crystalloid cardioplegic solution (St. Thomas formula, 10$^{\circ}C$, pH = 7.4) and reperfusate using isolated rat hearts in modified Langendorf apparatus by the parameters of preischemic and post reperfusing heart rate, time to first beat, occurance of arrhythmia, time to stabilization, and the rate of left ventricular pressure developing. Group A (n = 10), containing no FDP in cardioplegic and reperfusing solutions was control. Group B (n = 5), containing FDP in cardioplegic solution, showed statistically significant superiority of postischemic left ventricular pressure development than the control group. Group C (n = 5), containing FDP in reperfusate, showed statistically significant myocardial depressing effect than the controls. Other parameters were unremarkable. The cause is uncertain, but it is assumed that the negative feedback inhibition of FDP in energy metabolism or unknown blocking effect of FDP on certain transmembrane ionic currents is present. In conclusion, 1) FDP in cardioplegic solution has beneficial effect on postischemic left ventricular preservation. 2) FDP is strong acid when is hydrolyzed, so precise acid titration is neccessary. 3) FDP in reperfusate has negative left ventricular preservation, otherwise the mechanism is still uncertain.

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Production and Preservation of $\alpha$-Amylase from Bacillus sp. Y-124 (Bacillus sp. Y-124로부터 $\alpha$-Amylase의 생산 및 그 보존성에 관한 연구)

  • Jun, Hong-Ki;Yeehn Yeeh;Lee, Jong-Kune
    • Microbiology and Biotechnology Letters
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    • v.9 no.3
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    • pp.145-152
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    • 1981
  • Microorganisms from the waste water of starch industry, were isolated and a strain, Y-124, possessing a powerful enzymic activity was selected and identified as a member of the genus Bacillus. The ideal cultural condition for the formation of $\alpha$-amylase form Bacillus Y-124 and its preservation was investigated in connection with the biotechnological and industrial approach to the bulk enzyme production. High yield of $\alpha$-amylase was observed in medium containing casein as well as calcium pantothenate in this work. Calcium ions were found to have an effect in forming this particular enzyme. Ammonium phosphate dibasic was an important inorganic nitrogen source for the formation of $\alpha$-amylase. And preservation of this enzyme was greatly affected by calcium or sodium salts. The addition of calcium carbonate or sodium sulfate presented the most effective result for the prevention of its denaturation to various factors. The above data was obtained with crude enzyme preparation.

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Quality Effects of Various Pretreatment Methods on the Properties of Peeled Chestnut during Storage (깐밤의 전처리 방법이 저장 중 품질에 미치는 영향)

  • Kim, Jong-Hoon;Jeong, Jin-Woung;Kweon, Ki-Hyun
    • Food Science and Preservation
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    • v.14 no.5
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    • pp.462-468
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    • 2007
  • In this study, the quality effect of soaking in alum water, soaking in electrolyzed oxidizing water, and freezing during storage, on peeled chestnuts, were analyzed. When soaked in 0.1% (w/v) alum water, peeled chestnuts showed good characteristics in terms of weight loss, decomposition, and color preservation. However, texture and taste qualities rapidly decreased with increases in storage time. When soaked in twice their own weight of electrolyzed oxidizing water(pH 2.61, ORP 1,142 mV) for 10 min, the samples were preserved in an optimally edible condition. When frozen at $-10^{\circ}C$ for 5 min, the samples were suitable for use as material for processed chestnut produce, as was also the case when pretreatment with electrolyzed oxidizing water was employed.

Nutritional quality of Peucedanum japonicum Thunb. leaves in relation to ripening time, growing condition and blanching (재배조건, 수확시기 및 열처리에 따른 갯기름나물의 영양성분 변화)

  • Jin, Yong-Xie;Cho, Young-Sook;Choi, Youngmin
    • Food Science and Preservation
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    • v.21 no.6
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    • pp.784-789
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    • 2014
  • This work aimed to investigate the changes in the nutrient contents and antioxidant activity of Peucedanum japonicum Thunb. by ripening stage, growing condition, and blanching. The crude protein content of the young leaves (3.6~4.3%) was higher than that of the mature leaves (3.1~3.9%). Higher calcium contents were observed in the greenhouse-cultivated samples (225.9~259.2 mg/100 g) compared to the field-cultivated samples (178.5~199.5 mg/100 g). The vitamin C and folate contents (18.1~83.8 and 175.8~220.2 mg/100 g, respectively) of the field-cultivated samples were significantly (p<0.05) higher than those of the greenhouse-cultivated samples (13.1~57.7 and 133.0~148.8 mg/100 g, respectively). The growing condition and blanching were significant factors affecting the changes in the vitamin and polyphenol contents. The ${\beta}$ carotene contents of the blanched samples increased 2.6-fold compared to those of the raw samples. The total polyphenol contents (10.2~17.1 mg/g extract) and DPPH radical scavenging activity ($IC_{50}=2.0{\sim}3.0mg/ml$) of the blanched samples were significantly (p<0.05) higher than those of the raw samples (1.8~4.3 and $IC_{50}=16.2{\sim}21.1mg/ml$, respectively).

Effect of Storage Conditions on the Quality Stability of Garlic Bulbs (마늘의 품질안정성에 대한 저장조건의 영향)

  • 권중호;정형욱;이정은;박난영;이기동;김정숙
    • Food Science and Preservation
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    • v.6 no.2
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    • pp.137-142
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    • 1999
  • Physicochemical properties were evaluated for stored garlic bulbs during 9 months under different conditions, such as low-temperature condition (3${\pm}$1$^{\circ}C$, 80${\pm}$5% RH, LT), pit-temperature condition (9${\pm}$6$^{\circ}C$, 80${\pm}$5% RH,PT) and ambient-temperature condition (14${\pm}$11$^{\circ}C$, 67${\pm}$5% RH, AT). The internal sprout development was more significant in pit and ambient conditions than in low temperature and thus certain means for sprout control is required for long-term storage of garlic bulbs under such conditions. The rates of rotting and weight loss were appreciable especially after 7 months of storage (next March) in the order of PT, AT and LT, when external sprouts were observed in PT and AT. Moisture content of stored samples were relatively constant in LT until next May, but that in W and AT was significantly reduced after next January. Total sugars showed a decreasing tendency with the prolonged period, whereas an apparent increase was found in the contents of reducing sugar and vitamin C along with external sprouting of garlic bulbs from the 7th month of storage (next March). Based on the results that around March is a limiting point in garlic storage at such conditions from the physiological and physicochemical points of view, improved storage condition should be applied to overcome the storage barrier.

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Monitoring and Preventive Preservation of Cultural Heritages to Maintain Original Wooden Architectural Cultural Heritage (목조건축문화재 원형유지를 위한 문화재돌봄 모니터링과 예방보존)

  • CHUN Kyoungmee
    • Korean Journal of Heritage: History & Science
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    • v.56 no.4
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    • pp.192-214
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    • 2023
  • Wooden architectural cultural heritages are one of the visible legacies that show the national's identity. Even when the concept of 'the original' of cultural heritages was not accurately understood, the emphasis of preservation and management of cultural heritages was placed on 'preservation of the original form' or 'maintenance of the original form'. Moreover, these days, following the trend of international preservation principles, cultural heritages are considered important as "values as historical objects." This paper is the result of an attempt to determine the scope and content of what parts should be monitored to maintain the original form of wooden architectural cultural heritage. The first thing to be done in monitoring wooden architectural cultural heritage is to check the condition of the ground and foundation. The second is the column. This is because the instability of the column causes damage to the joint with each member and the fitting part, resulting in physical changes leading to damage to the wall. The third is monitor the roof tiles. If the leak continues into the building due to the separation or damage of the roof, the defect should be partially dismantled and repaired, so it should be monitored to maintain its original shape as much as possible. The monitoring range of the base, column, and roof serves as a reference point for identifying what damage is being done to the relevant cultural heritages. In other words, the data at the time when monitoring began becomes the 'original' for the year. Alternatives based on the analysis of monitoring for the preservation of original cultural heritages should be actively introduced. In addition, by sharing the current state and situation of cultural heritages as a result of monitoring with various related organizations, preventive preservation should be established rather than preservation of cultural heritages by "intervention."

Origin and Preservation Status of the 'Gongju Junghakdong Old Missionary House', the Registered Cultural Property No. 233 (등록문화재 제233호 '공주 중학동 구 선교사가옥'의 유래와 보존현황)

  • Suh, Mancheol;Kim, Sung Bae
    • Journal of Conservation Science
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    • v.34 no.1
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    • pp.11-21
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    • 2018
  • The result of the study on the origin and preservation status of the 'Gongju Junghakdong old missionary house', the Registered Cultural Property No. 233, reveals that the building was approved on October 23, 1921, and missionary Alice H. Sharp was living in the building until her retirement from her missionary life of 39 years in Korea in 1939. In order to review internal and external preservation status of the building, condition of wood material, the composition of the window, the damage of the wall, and the leaning of the building were examined. In particular, in the case of window facilities, it is necessary to restore it to the original upper and lower sliding window. As a result of investigation of the preservation status of the external facilities, it is necessary to restore the original shape of the staircase and a deck of the building on south western side and the well. In addition, the results of the non-destructive diagnosis of the ground revealed that the building was built on uneven surface layer of 2-5 m thickness and the boundary between the surface layer and the upper part of the weathered rock is inclined following geomorphology. This phenomenon shows that when the water content of the ground increases in the rainy season, the bearing capacity of the ground is lowered, and there is a possibility of uneven subsidence. Especially, landslides may occur in case of heavy rain. Therefore, it is desirable to install a masonry facility at the southwest boundary of the site, and it is recommended to install a drainage facility to ensure rapid drainage.

Effect of Modified Atmosphere Packaging Gas Composition on Quality Preservation of Korean Rockfish Fillets (조피볼락 필렛의 변형기체포장에서 품질보존에 미치는 기체조성의 영향)

  • Eo Jin Park;Su Chan Kim;Duck Soon An
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.29 no.3
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    • pp.145-151
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    • 2023
  • In order to extend shelf life for rockfish fillets by modified atmosphere packaging (MAP), different package atmospheres were compared in the product quality preservation. Firstly, CO2 solubility was measured at 0, 5, 10, and 15℃ to be incorporated into the mathematical model to predict the volume and CO2 concentration of the package at expected storage temperature. The CO2 solubility given in Henry's constant decreased with temperature to be fitted with a linear equation. Then air packaging as control and four MAP conditions of 100 g fillets were prepared and stored for duration of 5 days at 10℃ to compare them in the quality preservation effect. Four MAP conditions employed were CO2(60):O2(30):N2(10), CO2(60):O2(0):N2(40), CO2(30):O2(30):N2(40) andCO2(30):O2(0):N2(70). MAP conditions with high CO2 concentration inhibited total aerobic bacteria, and the conditions containing O2 led to low TVB-N. MAP of CO2(60):O2(30):N2(10) was found to be the best condition for rockfish fillet preservation considering total aerobic bacteria growth and nitrogenous volatiles production.

Effect of the concentrations of seminal plasma and aerobic or anaerobic condition on the motility of cooled equine semen (말 냉장 정액의 정장 비율과 호기 또는 혐기 조건이 운동성에 미치는 효과)

  • Park, Yong-Soo;Yang, Jae-Hyuk;Cho, Young-Jae;Kang, Ok-Deuk;Cho, Gil-Jae
    • Journal of Embryo Transfer
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    • v.33 no.1
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    • pp.23-30
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    • 2018
  • The purpose of this study was to investigate the effects of the concentration of seminal plasma in aerobic and anaerobic conditions on the total motility(TM) and the progressive motility(PM) of spermatozoa in long term preservation of cooled equine semen. We also examine the pregnancy rates after artificial insemination using fresh, cooled or frozen semen, and different durations of cooled-preserved equine semen. In the aerobic state of cooled-preserved semen, As the increase of preserved duration to 24h, 48h, 72h, and 96h, TM tended to decrease in each of different concentrations of formalin-containing experimental group, TM tended to decrease regardless of the concentrations of SP. In different concentrations of SP, TM of without seminal plasma(SP W/O) group tended to be higher than that of SP 20%, SP 33% and SP 50%, especially TM of SP W/O group was significantly higher than other groups at 96 h (p<0.05). PM was higher in the groups of SP W/O and SP 20% than in the groups of SP 33% and SP 50% from 24 h to 72 h in cooled-preservation, especially PM of SP W/O group was significantly higher than other groups at 96 h (p<0.05). In the anaerobic condition of cooled-preserved semen, the results of TM and PM at different concentrations of SP were similar to the results in the aerobic condition although there was a difference in the ratio. The pregnancy rates of fresh-cooled, cooled-preserved and frozen semen were 66.3%, 60.7% and 34.5%, respectively, and the pregnancy rate of frozen semen was the lowest. We also found that it is possible to pregnancy after artificial insemination using 72 h cooled-preserved equine semen. There was similar of the pregnancy rates in the different month from April to August.

Combined Effect of Heat Treatment and Gamma Irradiation on the Shelf-Stability and Quality of Packaged Kimchi during Accelerated Storage Condition (가열 및 감마선 조사 병용처리가 가속저장 포장 김치의 저장안정성 및 품질에 미치는 영향)

  • Kim, Mi-Jung;Park, Jin-Gyu;Kim, Jae-Hun;Park, Jae-Nam;Lee, Hyun-Joo;Kim, Wang-Geun;Lee, Ju-Woon;Byun, Myung-Woo
    • Food Science and Preservation
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    • v.13 no.5
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    • pp.531-537
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    • 2006
  • This study was conducted to evaluate the combined effect of heating and gamma irradiation on the shelf-stability and quality of kimchi during storage at $35^{\circ}C$. The optimum condition for heat treatment of kimchi was considered at $60^{\circ}C$ for 30 min. Irradiation after kent treatment was effective to inhibit the growth of microorganisms and prevent the quality change of kimchi during storage. In particular, microorganisms of kimchi co-treated with heat and 20 kGy-irradiation were not detected and sensory scores of that were the highest after storage at $35^{\circ}C$ for 14 days. Therefore, combination treatment of heat and irradiation was considered as the effective method to improve the shelf-stability of kimchi in the accelerated condition.