• Korean Journal of Pharmacognosy
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• v.40 no.1
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• pp.18-24
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• 2009

GCSB-5 preparation is a purified extract from a mixture six herbal medicines (Acanthopanacis Cortex, Achyranthis Radix, Saposhnikoviae Radix, Cibotii Rhizoma, Glycine Semen Nigra, Eucommiae Cortex) that have been widely used in traditional medicine to treat various bone disorders. This study was carried out to obtain the HPLC analysis method that can be used to establish quantitative analysis of Glycine Semen Nigra and Eucommiae Cortex for standardization of GCSB-5 preparation. HPLC analysis methods for the simultaneous determination of genistin (Glycine Semen Nigra) and geniposide (Eucommiae Cortex) were established for the quality control of herbal medicinal raw material and preparation. And validation of HPLC analysis methods were conformed for verification of HPLC methods by check to specificity, linearity, intra-day precision, inter-day precision and accuracy following ICH guideline. As the result of quantitative analysis, the contents of genistin and geniposide in the raw material of GCSB-5 preparation were 0.0426-0.0427 mg/g and 0.431-0.432 mg/g. And GCSB-5 preparation contained genistin of 0.0202-0.0203 mg/capsule and geniposide of 0.211-0.212 mg/capsule, respectively.

• Restorative Dentistry and Endodontics
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• v.48 no.1
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• pp.1.1-1.11
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• 2023

Objectives: This study evaluated the dentinal penetration depth of 2.5% sodium hypochlorite (NaOCl) in root canals with and without preparation and different irrigant activation protocols. Materials and Methods: Sixty-three bovine mandibular incisors were randomly allocated to 6 groups (n = 10): G1, preparation + conventional needle irrigation (CNI); G2, preparation + passive ultrasonic irrigation (PUI); G3, preparation + Odous Clean (OC); G4, no preparation + CNI; G5, no preparation + PUI; G6, no preparation + OC; and CG (negative control; n = 3). Samples were filled with crystal violet for 72 hours. Irrigant activation was performed. Samples were sectioned perpendicularly along the long axis, 3 mm and 7 mm from the apex. Images of the root thirds of each block were captured with a stereomicroscope and analyzed with an image analysis software. One-way analysis of variance, followed by the Tukey post hoc test, and the Student's t-test were used for data analysis, with a significance level of 5%. Results: The NaOCl penetration depth was similar when preparation was performed, regardless of the method of irrigation activation (p > 0.05). In the groups without preparation, G6 showed greater NaOCl penetration depth (p < 0.05). The groups without preparation had a greater NaOCl penetration depth than those with preparation (p = 0.0019). Conclusions: The NaOCl penetration depth was similar in groups with root canal preparation. Without root canal preparation, OC allowed deeper NaOCl penetration. The groups without preparation had greater NaOCl penetration than those undergoing root canal preparation.

• The Korean Journal of Emergency Medical Services
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• v.16 no.3
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• pp.91-102
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• 2012

Purpose : This study was designed to examine the effects of self-esteem and adjustment to college life on career preparation behavior in 209 paramedic students attending two colleges in G Metropolitan City and provide the basic materials of career development program. Methods : Data were collected from Sep. 7 to 12, 2011 and SEI Form B made by Coopersmith, adjustment to college life by Baker and Siryk, and career preparation behavior by Kim were adopted and revised. Results : There was significant difference in self-esteem and adjustment to college life between gender, but there was no difference in career preparation behavior. Self-esteem and career preparation behavior were graders difference, but there was no in adjustment to college life. There were positive correlations between self-esteem and adjustment to college life. The most significant influence on career preparation behavior of paramedic students was academic adjustment, followed the social and emotional adjustment. Conclusion : For the effective career preparation behaviors, career guidance methods and programs that induce academic, social and emotional adjustment to be available in adjustment to college life of gender and grade.

• Korean journal of food and cookery science
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• v.11 no.3
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• pp.220-225
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• 1995

This study was attempted to standardize preparation methods of Korean foods; Chigae (Stew) such as Deonjang chigae (soybean stew) and Kimchi chigae (Kimchi stew). We followed cooking methods by using cookbooks, food service operation recipes, materials distributed by commercial food restaurants from their own experimental kitchens, and home recipes. Before this recipes were standardized, we thoroughly analyzed and modified them. The total yield and portions were determined together with material weights and procedures. And we evaluated food made by standardized recipes for acceptability, by taste panels from Sookmyung Women＇s University. It concluded that yield volune for doenjang chigae and kimchi chigae after cooking for 10 persons was 3 kg, optimum service temperature was 7$0^{\circ}C$, preparation time was 50 minutes, service volumes by one person was 300 g. This presentantion for the above on food materials and material weights, preparation methods as well as cautions and references are attached in this research paper.

• Applied Microscopy
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• v.45 no.4
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• pp.218-224
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• 2015

A technical overview on the various sample preparation methods for electron backscattered diffraction (EBSD) analysis is carried out. The mechanical polishing with colloidal silica finish, electro-chemical polishing, dual layer coating and ion beam milling are introduced for the common sample preparation methods for EBSD observation and some issues that are frequently neglected by the common EBSD users but should be considered to get a reliable EBSD data are discussed. This overview would be especially helpful to the people who know what EBSD technique is but do not get a reliable EBSD data because of difficulties in sample preparation.

• Journal of Korean Academy of Fundamentals of Nursing
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• v.24 no.1
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• pp.60-71
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• 2017

Purpose: The purpose of this study was to develop video assisted education on bowel preparation for colonoscopy (VEBPC) and use a snartphone to evaluate effects of the VEBPC. Methods: Adult patients who were scheduled for colonoscopy were recruited from a university general hospital and randomly assigned to three groups. Group 1 (n=30) watched the video using a computer set in the endoscope consulting room. Group 2 (n=29) watched it using a smartphone, and group 3, the control group (n=29) received education with existing instructions at the reservation-reception desk. Participants were evaluated on knowledge on taking bowel preparation agents and diet, compliance on taking bowel preparation agents and diet, satisfaction with education, and actual level of bowel preparation. Results: Group 1 and 2 showed significantly (p<.001) higher scores for knowledge, compliance, and satisfaction compared to the control group. However, in post-hoc test analyses there were no significant differences in these variables between group 1 and 2. No significant difference was found in the actual level of bowel preparation among the three groups. Conclusion: Findings from this study show that VEBPC using smartphone is a better option than existing educational methods. However, replication studies are necessary to confirm these findings.

• Clinical and Experimental Reproductive Medicine
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• v.18 no.1
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• pp.95-99
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• 1991

Swim-up and Wang's tube system are known methods of motile sperm selection and aerobic bacterial removal from the raw semen. This study was designed to evaluate the recovery rate of motile sperm, % normal morphology of sperm, the efficiency of bacterial removal after sperm preparation by the above two methods. The results were as follows. 1. There was more significant increase of sperm concentration in preparation by swim-up than Wang's tube (p<0.05). The concentration of sperm by swim up was changed from $82.5{\times}10^6/ml$ to $6836{\times}10^6/ml$, and Wang's tube was changed from $82.5{\times}10^6/ml$ to $36.0{\times}10^6/ml$. 2. There was significant increase in sperm motility after preparation by two methods in comparison with initial sperm motility (p<0.05), but no statistical difference between two methods was noted. The % motility of sperm by swim-up was increased from 66.1% to 95.7% and Wang's tube from 66.1% to 98.1%. 3. There was significant increase of % normal morphology of sperm in the samples prepared by two methods (p<0.05), from 49.2% to 85.3% in swim-up and from 49.2% to 92.1% in Wang's tube, but there was no statistical significance between them. 4. There was no bacterial growth in aerobic culture after preparation by two methods.

• Clinical and Experimental Reproductive Medicine
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• v.42 no.1
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• pp.22-29
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• 2015

Objective: Sperm must be properly prepared in in vitro fertilization (IVF)-embryo transfer (ET) programs in order to control the fertilization rate and ensure that embryos are of high quality and have appropriate developmental abilities. The objective of this study was to determine the most optimal sperm preparation method for IVF. Methods: Patients less than 40 years of age who participated in a fresh IVF-ET cycle from November 2012 to March 2013 were included in this study. Poor responders with less than three mature oocytes were excluded. Ham's F-10 medium or sperm-washing medium (SWM) was used in combination with the density-gradient centrifugation/swim-up (DGC-SUP) or SUP methods for sperm preparation. A total of 429 fresh IVF-ET cycles were grouped according to the media and methods used for sperm preparation and retrospectively analyzed (DGC-SUP/Ham's F-10, n=82; DGC-SUP/SWM, n=43; SUP/Ham's F-10, n=181; SUP/SWM, n=123). Results: There were no significant differences among these four groups with respect to the mean age of the female partners, duration of infertility, number of previous IVF cycles, and retrieved oocytes. We determined that both the DGC-SUP and SUP methods for sperm preparation from whole semen, using either Ham's F-10 or SWM media, result in comparable clinical outcomes, including fertilization and pregnancy rates. Conclusion: We suggest that both media and both methods for sperm preparation can be used for selecting high-quality sperm for assistive reproductive technology programs.

• Food Engineering Progress
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• v.21 no.3
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• pp.191-200
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• 2017

There have been great efforts to develop a rapid and sensitive detection method to monitor the presence of pathogenic bacteria in food. While a number of methods have been reported for bacterial detection with a detection limit to a single digit, most of them are suitable only for the bacteria in pure culture or buffered solution. On the other hand, foods are composed of highly complicated matrices containing carbohydrate, fat, protein, fibers, and many other components whose composition varies from one food to the other. Furthermore, many components in food interfere with the downstream detection process, which significantly affect the sensitivity and selectivity of the detection. Therefore, isolating and concentrating the target pathogenic bacteria from food matrices are of importance to enhance the detection power of the system. The present review provides an introduction to the representative sample preparation strategies to isolate target pathogenic bacteria from food sample. We further describe the nucleic acid-based detection methods, such as PCR, real-time PCR, NASBA, RCA, LCR, and LAMP. Nucleic acid-based methods are by far the most sensitive and effective for the detection of a low number of target pathogens whose performance is greatly improved by combining with the sample preparation methods.

• The Journal of Korean Society for School & Community Health Education
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• v.15 no.1
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• pp.45-59
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• 2014

Objectives: This study is designed to evaluate the influential factors of career decision-making self-efficacy and career identity by health science college students on their job preparation beaviors. Methods: The total 714 questionnaires were distributed to juniors and seniors of health science college, and the result was statistically analyzed through IBM SPSS statistics 20.0. Results: 1. There was no significant difference of job preparation behavior between male students and female students, and while seniors showed highly active job preparation behaviors, those who had high satisfaction level toward their major studies, high academic achievement, and outgoing personality had highly active job preparation behaviors. 2. Students with high satisfaction level toward their major studies, high academic achievement, outgoing personality, and high expected salaries showed high career decision-making self-efficacy level. 3. Students with high satisfaction level toward their major studies, high academic achievement and highly outgoing personality showed high level of career identity. 4. Subsidiary factors of career decision-making self-efficacy were collection of job information, goal setting, planning, and self-assessment, and all of them showed correlation with job preparation behaviors. 5. Among subsidiary factors of career decision-making self-efficacy, "self-understanding" and understanding of job information showed correlation with job preparation behaviors. Conclusion: For improvement on career decision-making self-efficacy and career identity of health science college students, individual support, psychological support, educational support and other supports regarding job employment to provide wide paths and methods for job preparation behaviors are essential, and through various job employment education program and graded counseling, job preparation behaviors shall be promoted as a great strategy.