• Title, Summary, Keyword: Preparation methods

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Quantitative Analysis of Glycine Semen Nigra and Eucommiae Cortex for Standardization of GCSB-5 Preparation (생약복합제 GCSB-5의 품질 표준화를 위한 흑두 및 두충의 함량 분석)

  • Lee, Eun-Hee;Cha, Bae-Cheon
    • Korean Journal of Pharmacognosy
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    • v.40 no.1
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    • pp.18-24
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    • 2009
  • GCSB-5 preparation is a purified extract from a mixture six herbal medicines (Acanthopanacis Cortex, Achyranthis Radix, Saposhnikoviae Radix, Cibotii Rhizoma, Glycine Semen Nigra, Eucommiae Cortex) that have been widely used in traditional medicine to treat various bone disorders. This study was carried out to obtain the HPLC analysis method that can be used to establish quantitative analysis of Glycine Semen Nigra and Eucommiae Cortex for standardization of GCSB-5 preparation. HPLC analysis methods for the simultaneous determination of genistin (Glycine Semen Nigra) and geniposide (Eucommiae Cortex) were established for the quality control of herbal medicinal raw material and preparation. And validation of HPLC analysis methods were conformed for verification of HPLC methods by check to specificity, linearity, intra-day precision, inter-day precision and accuracy following ICH guideline. As the result of quantitative analysis, the contents of genistin and geniposide in the raw material of GCSB-5 preparation were 0.0426-0.0427 mg/g and 0.431-0.432 mg/g. And GCSB-5 preparation contained genistin of 0.0202-0.0203 mg/capsule and geniposide of 0.211-0.212 mg/capsule, respectively.

Effects of self-esteem and adjustment to college life on career preparation behavior of paramedic students (응급구조과 학생의 자아존중감과 대학생활적응이 진로준비행동에 미치는 영향)

  • Choi, Gil-Soon;Lee, Chang-Hee
    • The Korean Journal of Emergency Medical Services
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    • v.16 no.3
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    • pp.91-102
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    • 2012
  • Purpose : This study was designed to examine the effects of self-esteem and adjustment to college life on career preparation behavior in 209 paramedic students attending two colleges in G Metropolitan City and provide the basic materials of career development program. Methods : Data were collected from Sep. 7 to 12, 2011 and SEI Form B made by Coopersmith, adjustment to college life by Baker and Siryk, and career preparation behavior by Kim were adopted and revised. Results : There was significant difference in self-esteem and adjustment to college life between gender, but there was no difference in career preparation behavior. Self-esteem and career preparation behavior were graders difference, but there was no in adjustment to college life. There were positive correlations between self-esteem and adjustment to college life. The most significant influence on career preparation behavior of paramedic students was academic adjustment, followed the social and emotional adjustment. Conclusion : For the effective career preparation behaviors, career guidance methods and programs that induce academic, social and emotional adjustment to be available in adjustment to college life of gender and grade.

Standardization of Recipe for the Preparation of Korean Foods (II) - For the focus on Chigae (Stew) - (한국음식의 조리법 표준화를 위한 연구(II) -찌개류 -)

  • 계승희;문현경;염초애;송태희;이성희
    • Korean journal of food and cookery science
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    • v.11 no.3
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    • pp.220-225
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    • 1995
  • This study was attempted to standardize preparation methods of Korean foods; Chigae (Stew) such as Deonjang chigae (soybean stew) and Kimchi chigae (Kimchi stew). We followed cooking methods by using cookbooks, food service operation recipes, materials distributed by commercial food restaurants from their own experimental kitchens, and home recipes. Before this recipes were standardized, we thoroughly analyzed and modified them. The total yield and portions were determined together with material weights and procedures. And we evaluated food made by standardized recipes for acceptability, by taste panels from Sookmyung Women's University. It concluded that yield volune for doenjang chigae and kimchi chigae after cooking for 10 persons was 3 kg, optimum service temperature was 7$0^{\circ}C$, preparation time was 50 minutes, service volumes by one person was 300 g. This presentantion for the above on food materials and material weights, preparation methods as well as cautions and references are attached in this research paper.

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Technical Overview on the Electron Backscattered Diffraction Sample Preparation

  • Kim, Dong-Ik;Kim, Byung-Kyu;Kim, Ju-Heon
    • Applied Microscopy
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    • v.45 no.4
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    • pp.218-224
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    • 2015
  • A technical overview on the various sample preparation methods for electron backscattered diffraction (EBSD) analysis is carried out. The mechanical polishing with colloidal silica finish, electro-chemical polishing, dual layer coating and ion beam milling are introduced for the common sample preparation methods for EBSD observation and some issues that are frequently neglected by the common EBSD users but should be considered to get a reliable EBSD data are discussed. This overview would be especially helpful to the people who know what EBSD technique is but do not get a reliable EBSD data because of difficulties in sample preparation.

Improved Accuracy of Cytodiagnosis using the Kato Self-Collection Devise: the Usefulness of Smear Preparation in Liquid-based Cytology Methods

  • Okayama, Kaori;Okodo, Mitsuaki;Fujii, Masahiko;Kumagai, Tomoko;Yabusaki, Hiromi;Shiina, Yoshio;Iwami, Fumihiro;Teruya, Koji;Hatta, Kenmei
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.9
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    • pp.4521-4524
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    • 2012
  • Object: In the present study, we compared the positive cytodiagnostic test rates with discrepancies using self-collection devices for cervical cancer screening. We made this survey to examine whether or not our self-smear preparation method using the Kato self-collection device contributed to an improved rate of detecting atypical cells compared with existing recommended preparation methods. Methods: Specimens were collected at 14 facilities handling self-collection methods, and samples were collected by a physician in 2 facilities. The chisquared test was performed using the SPSS ver. 20 statistical software to determine the relationships between the positive cytodiagnostic rate, specimen preparation methods, and self-collection devices. Results: Collecting cells using the Kato self-collection device and preparing liquid-based specimens, we obtained a significantly higher rate of positive cytodiagnosis and our results were equal to those obtained with the direct method. Conclusions: Taking into consideration increased needs for screening using the self-collection method in future, with even more improved test accuracy, a screening test that is acceptable to society needs to be established.

Effects of Video Assisted Education Using Smartphone on Bowel Preparation for Colonoscopy (스마트폰을 이용한 대장내시경 장정결 동영상 교육의 효과)

  • Choi, Mi-Hee;Song, Jun-Ah
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.24 no.1
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    • pp.60-71
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    • 2017
  • Purpose: The purpose of this study was to develop video assisted education on bowel preparation for colonoscopy (VEBPC) and use a snartphone to evaluate effects of the VEBPC. Methods: Adult patients who were scheduled for colonoscopy were recruited from a university general hospital and randomly assigned to three groups. Group 1 (n=30) watched the video using a computer set in the endoscope consulting room. Group 2 (n=29) watched it using a smartphone, and group 3, the control group (n=29) received education with existing instructions at the reservation-reception desk. Participants were evaluated on knowledge on taking bowel preparation agents and diet, compliance on taking bowel preparation agents and diet, satisfaction with education, and actual level of bowel preparation. Results: Group 1 and 2 showed significantly (p<.001) higher scores for knowledge, compliance, and satisfaction compared to the control group. However, in post-hoc test analyses there were no significant differences in these variables between group 1 and 2. No significant difference was found in the actual level of bowel preparation among the three groups. Conclusion: Findings from this study show that VEBPC using smartphone is a better option than existing educational methods. However, replication studies are necessary to confirm these findings.

Incidence and Predictors of Inadequate Bowel Preparation before Elective Colonoscopy in Thai Patients

  • Bhanthumkomol, Patommatat;Siramolpiwat, Sith;Vilaichone, Ratha-Korn
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.24
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    • pp.10763-10768
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    • 2015
  • Background: The incidence of inadequate bowel preparation before elective colonoscopy in this region has never been studied. Materials and Methods: The authors prospectively described the incidence and factors related to inadequate bowel preparation in Thammasat university hospital. Bowel preparation quality was accessed by using Aronchick scale. Factors associated with inadequate bowel preparation were also recorded. Results: Two hundred patients undergoing elective outpatient colonoscopy were enrolled. Inadequate and fair bowel preparation was documented in 9 and 43%, respectively. Factors associated with inadequate bowel preparation were incomplete cleansing agent ingestion (odds ratio 7.7; 95%CI 1.62-36.64) and patient's unrecognization of vegetable avoidance (odds ratio 3.26; 95%CI 1.14-9.28). Conclusions: Compared with previous reported, inadequate bowel preparation was seen less in our study, however, more patients with fair bowel preparation was documented. Further study aiming at investigating the type and amount of fiber contained in diet before elective colonoscopy should be commenced.

A Proposal for Prototype-Free Production Preparation Processes Utilizing 3DCG Animations

  • Shinoda, Shinji;Shimozawa, Kazuhiro;Niwa, Akira;Kawase, Takeshi;Matsumoto, Toshiyuki;Mizumachi, Tadahiro
    • Industrial Engineering and Management Systems
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    • v.8 no.2
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    • pp.109-120
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    • 2009
  • As the use of 3DCAD data became widespread in designing products in manufacturing, attempts have been made to shorten lead time and reduce cost of production preparation utilizing 3DCAD data for launching assembly lines. In order to create assembly plans not only efficient but easy for operators to operate ('easy-to-operate'), this study presents approaches, methods, and systems for creating 3DCGAs (3 Dimensional Computer Graphic Animations) which automatically utilize a prototype-free production preparation methodology. Characteristics of this study include that it proposes the methodology for creating assembly operation 3DCGAs automatically, for all the possible assembly operations corresponding each of the possible assembly sequences first. Using the created 3DCGAs, the study next considers assembly methods by evaluating how easy or 'operator friendly' they are in implementing, and devises tools or jigs to be used, and plans efficient assembly line organization. The concept of the methodology was formed by focusing on the value-adding assembly steps at which parts turn into products directly. The study also validates the effectiveness of the presented methodology by employing the methods used in actual production preparation process in businesses, and proves that an efficient assembly line can be organized in a shorter period of time utilizing the developed system and by preparing easy-to-operate and efficient plans in 3DCGAs at the design stage.

A Study of Effects of Wang's Tube in Semen Preparation (정자 준비에서의 Wang's Tube 효과에 관한 연구)

  • Kim, Young-Tae;Kim, Yong-Ook;Kim, Hae-Jung;Kim, Sun-Haeng;Rha, Joong-Yol;Ku, Pyong-Sahm
    • Clinical and Experimental Reproductive Medicine
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    • v.18 no.1
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    • pp.95-99
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    • 1991
  • Swim-up and Wang's tube system are known methods of motile sperm selection and aerobic bacterial removal from the raw semen. This study was designed to evaluate the recovery rate of motile sperm, % normal morphology of sperm, the efficiency of bacterial removal after sperm preparation by the above two methods. The results were as follows. 1. There was more significant increase of sperm concentration in preparation by swim-up than Wang's tube (p<0.05). The concentration of sperm by swim up was changed from $82.5{\times}10^6/ml$ to $6836{\times}10^6/ml$, and Wang's tube was changed from $82.5{\times}10^6/ml$ to $36.0{\times}10^6/ml$. 2. There was significant increase in sperm motility after preparation by two methods in comparison with initial sperm motility (p<0.05), but no statistical difference between two methods was noted. The % motility of sperm by swim-up was increased from 66.1% to 95.7% and Wang's tube from 66.1% to 98.1%. 3. There was significant increase of % normal morphology of sperm in the samples prepared by two methods (p<0.05), from 49.2% to 85.3% in swim-up and from 49.2% to 92.1% in Wang's tube, but there was no statistical significance between them. 4. There was no bacterial growth in aerobic culture after preparation by two methods.

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Comparison of the effect of different media on the clinical outcomes of the density-gradient centrifugation/swim-up and swim-up methods

  • Kim, Eun-Kyung;Kim, Eun-Ha;Kim, Eun-Ah;Lee, Kyung-Ah;Shin, Ji-Eun;Kwon, Hwang
    • Clinical and Experimental Reproductive Medicine
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    • v.42 no.1
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    • pp.22-29
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    • 2015
  • Objective: Sperm must be properly prepared in in vitro fertilization (IVF)-embryo transfer (ET) programs in order to control the fertilization rate and ensure that embryos are of high quality and have appropriate developmental abilities. The objective of this study was to determine the most optimal sperm preparation method for IVF. Methods: Patients less than 40 years of age who participated in a fresh IVF-ET cycle from November 2012 to March 2013 were included in this study. Poor responders with less than three mature oocytes were excluded. Ham's F-10 medium or sperm-washing medium (SWM) was used in combination with the density-gradient centrifugation/swim-up (DGC-SUP) or SUP methods for sperm preparation. A total of 429 fresh IVF-ET cycles were grouped according to the media and methods used for sperm preparation and retrospectively analyzed (DGC-SUP/Ham's F-10, n=82; DGC-SUP/SWM, n=43; SUP/Ham's F-10, n=181; SUP/SWM, n=123). Results: There were no significant differences among these four groups with respect to the mean age of the female partners, duration of infertility, number of previous IVF cycles, and retrieved oocytes. We determined that both the DGC-SUP and SUP methods for sperm preparation from whole semen, using either Ham's F-10 or SWM media, result in comparable clinical outcomes, including fertilization and pregnancy rates. Conclusion: We suggest that both media and both methods for sperm preparation can be used for selecting high-quality sperm for assistive reproductive technology programs.