• Preventive Nutrition and Food Science
• /
• v.5 no.2
• /
• pp.75-80
• /
• 2000

To separate ACE inhibitors from edible plants, spices, and herbs, 285 extracts of 95 sources were screened for ACE inhibitory activity. The extract of Sinapis alba L. had the most potent ACE inhibitory activity. Mustard seeds were crushed homogeneously and extracted with hexane and water successively. Lyophilized water extract was fractionated with $H_2O$:butanol(1:1). The ACE inhibitor was purified from butanol fraction by methanol precipi-tation, gel filtration, HPLC, and FPLC with Superdex peptide HQ 10/30 column. The active fraction has been purified to homogeneity, which was proven by gel filtration using FPLC system. The yield was 0.02%. The com-pound has a molecular weight of about 640. The compound competitively inhibited ACE activity and the $IC_{50}$ value was 79$\mu\textrm{g}$/ml. The purified compound showed uterus contraction activity in isolated rat uterus.

• Current Research on Agriculture and Life Sciences
• /
• v.5
• /
• pp.143-157
• /
• 1987

In order to determine the design precipitation, the most probable daily precipitation and annual precipitation at every spot are calculated and iso - precipitation line are drawn. Probability of precipitation and drought phenomena of each gage station are analyzied by the method of frequency analysis from the statistical conceptions. The results summarized in this study are as the follows. 1. Annual mean precipitation in kyungpook area are 1044 mm, about 115 mm less than annual mean precipitation of Korea amounts to l1S9mm, and found to regionally unequal. 2. Monthly mean rainfall of July is 242.2mm, 23.2%, August 174.2mm, 16.7%, June 115mm, 11% and September 114.2mm, 10.9% and Rainfall depth of July-August are more than 40% of annual precipition. This shows notable summer rainy weather by typoon and low pressure storm and seasonal unbalance of water supply. 3. The relation among the maximum precipi.tation per day, per two continuous days and per three contnous days are caculated and the latter is found 31.0% increased rate of the first and the last 48.2% increased rate of first. 4. Probability precipitation in Kyungpook area are shown as 9.0%(5 year), 13.3%(10 year), 17.7%(20 year), 23.1%(50 year), 27.0%(100 year) and 31.1%(200 year) increased rate of each recurrence year compared with observed average annual precipitation. 5. From annual precipitation and maximum daily rainfall data probability of precipitation and precipitation isohyetal line are derived which shown as Table 11 and Fig. 8. 6. Drought days are divided 6 class and analysed results are shown on table 12. Average occurrence time of 10-14 continuous drought days are 2.3 time per year, 15-19 days are 0.9 time per year, 20-24 days are one per six years, 30-34 days are once per nine years and over than 35days are once per 25 years.

• Journal of Korean Society of Environmental Engineers
• /
• v.22 no.11
• /
• pp.2007-2016
• /
• 2000

After kaolin clay was compulsorily contaminated with $Sr^{2+}$ ion. the remediation characteristics by electrokinetic method were analyzed. In the first experiment. NaCl solution was used as an electrolyte to raise the electric field strength. After remediation for 0.8 days. the pH of the cathode side of the soil column was elevated from 4.0 to 11.7. and thereby precipitation $Sr(OH)_2$, started to be formed in the side. Therefore. efficiency of soil remediation was reduced significantly and the 32% of total $Sr^{2+}$ ion in the column was decontaminated in 6.7 days. In the second experiment. ethanoic buffer was injected in the soil column and $CH_3COOH$ was continuously inputted in cathode reservoir to restrain the pH elevation. The pH of the cathode side of the soil column was only ascended from 4.0 to 6.0 in 3.8 days and $Sr(OH)_2$ was not formed. The 21% of total $Sr^{2+}$ in the soil column was decontaminated in 0.6 days. and the 33% of total $Sr^{2+}$ in 0.9 days. and the 84% of total $Sr^{2+}$ in 1.6 days. and the 92% of total $Sr^{2+}$ in 2.5 days. and the 97% of total $Sr^{2+}$ in 3.8 days. Meanwhile. the residual concentrations in the column calculated by the developed model were similar to those by experiment.