Citrus melanose is one of important disease in citrus cultivation, reducing quality of citrus fruits and resulting in economic loss. Like other diseases in citrus, melanose was mainly protected by chemical fungicide in the field. Recently, alternative method of disease control is highly required due to the side effect of the chemicals. In this study four rhizobacterial strains TRH423-3, MRL408-3, THJ609-3, and TRH415-2 are selected by dual-culture testing its antifungal activity against Diaporthe citri causing citrus melanose. To investigate the protection efficacy of the selected rhizobacterial strains to citrus melanose, the bacteria were pre-treated on citrus leaves following inoculation with melanose pathogen. Pre-treatment with all selected rhizobacterial strains showed disease suppression in which the levels of protection rates were different by the rhizobacterial strains. Additional treatment with the rhizobacterial strains after the pathogen inoculation enhanced protection rates in all cases. The strain MRL408-3 and TRH423-3 were identified as Burkholderia gladioli, TRH415-2 as Pseudomons fluorescens and THJ609-3 as Pseudomonas pudia as a result of analyzing the internal transcript spaces of the rhizobacterial strains rDNA. The selected rhizobacterial strains may be valuable as biological control agents in the environment-friendly citrus farm in which chemical application is limited.
Lee Dong-Woon;Choi Woo-Geun;Lee Sang-Myeong;Choo Ho-Yul;Kweon Tae-Woong
Korean journal of applied entomology
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v.45
no.1
s.142
/
pp.67-74
/
2006
Korean entomopathogenic nematodes were introduced into cultural management of turfgrasses to enhance white grub control in golf courses for saving labour and expenses and contribution to giving safe playing and working places for golfers and superintendents by environmentally friendly control strategy. The turfgrass height influenced efficacy of entomopathogenic nematodes. Efficacy was higher at short turfgrass both in pot using Galleria mellonella larvae and in golf courses using 2nd instar of Exomala orientalis and Ectinohoplia rufipes as baits. Aeration increased the efficacy of entomopathogenic nematodes which were lower virulent. Pre-aeration was more effective than post-aeration. Exomala orientalis larval population was reduced 80.4 and 66.0% in the pre-aeration and post-aeration with entomopathogenic nematode, Steinernema carpocapsae Pocheon strain compared to 35.4% in the no aeration treatment.
Khaled H. El-Kholy;Hasan Tag El-Din;Found A. Tawfeek;Vincenzo Tufarelli;Caterina Losacco;Rashed A. Alhotan;Manal E. Shafi;Mohamed A. Korish;Youssef A. Attia;Sara H. M. Hassab
Animal Bioscience
/
v.37
no.5
/
pp.896-907
/
2024
Objective: The potential of aqueous extract of Christ's thorn jujube (Ziziphus spina-christi) leaves (SLAE) to reduce the negative impacts of heat stress on production performance and physiological traits was investigated in dual-purpose layers under subtropical farming. Methods: A total of 200, 25-week-old laying hens (Inshas strain) were randomly assigned to four dietary treatments including SLAE at 0, 2.5, 5.0, and 7.5 mL/kg, respectively. The average temperature-humidity index value was 26.69 during the experimental period. The SLAE contained saponin (0.045%), total flavonoid content of 17.9 mg of quercetin equivalent/100 g and overall antioxidant capacity concentration of 17.9 mg of ascorbic acid equivalent/100 g. Results: The maximum final body weight (BW), BW gain, egg weight, number, and mass occurred at the level of SLAE7.5 inclusion. The egg quality was significantly higher in SLAE groups than in control, and overall, SLAE7.5 had the most favorable influence at 28 and 32 weeks. Liver and kidney function, as well as lipids profile, improved significantly by SLAE inclusion; the lowest concentrations of these parameters were in SLAE7.5 hens. Treatment with SLAE7.5 increased total antioxidant capacity and endogenous antioxidant enzymes compared to control, whereas no effect on superoxide dismutase was noticed. Conclusion: The addition of SLAE at 7.5 mL/kg diet improved egg laying performance and quality, metabolic profiles, and antioxidant status during hyperthermia conditions.
Soldering is the usual method used to correct an unstable fixed partial denture framework at patient's try-in; However, presoldering base metal alloys is technique-sensitve and results are unstable because it is difficult to maintain uniform heat distribution and to prevent oxidation of an alloy. A cast-joining technique has been developed by Weiss and Munyon for repair, correction and addition to base metal framework. This joining technique eliminates the problem with presoldering of non-precious frameworks. The object of this study was to 1) compare the relative flexure strength and the joining effectiveness of Ni-Cr-Be cast in two pieces and 'pre-soldered' versus in two pieces and 'cast-joined'. 2) determine the effect of increasing the number of retentive grooves on the face of the cast and 3) determine the effect of the relative matched position of groove patterns on flexure strength. The joining effectiveness can be expressed by the ratio of the mean flexure stress of soldered or cast-joined specimens to that of one-piece cast. Resin rods 3mm in diameter were used as pattern of specimens for one-piece casted, presoldered, and cast-joined groups. Cast-joined specimens had two different patterns of retentive grooves on the joined faces. Type A had cross-shaped grooves 1mm in depth. 0.6mm in width. Type B was the same except for the addition of one more retentive groove. In the experiment connecting cast-joined specimens, half of specimens with type A pattern had their patterns on the faces of paired casts matched with each other as mirror image. With the rest pairs, it was proceeded that one of paired casts turned 45 degrees so that the patterns crossed. Half of specimens with type B pattern also had the patterns matched as mirror image; However, here, one of paired casts turned 90 degrees with the other pairs. Retentive groove in this study lacked the intentional undercuts, in contrast with the suggestion of Weiss and Munyon. The specimens were subjected to four-point flexural loading in an Instron testing machine. The midspan flexural stress was calculated at the point of initial plastic strain as determined from a strip-chart recorder or at the point of failure if this occured at a lower stress level. Within the scope of this study, the following results were obtained. 1. The presoldered group showed flexural strength at least 2 times higher than the cast-joined groups. Its joining effectiveness was 82%. 2. In cast-joined groups, the flexural strength of joints with type B patterns exhibited 1.5 times that of joints with type A patterns. Joining effectivenesses were 38% for type B patterns, 25-26% for type A patterns. 3. The relative matched position of groove patterns did not have any significant effect on flexural strength of the cast-joined specimens with either type A patterns or type B patterns(p>.05).
In an attempt to measure effects of the Programmed Resistance Exercise on the daily performance of patients with rheumatoid arthritis, a quasi-experiment ; non-synchronised control group pre-post-test, was carried out for 8 weeks : from June 3 to November 29, 1996. 25 for the experimental group and 26 for the control group were conveniently sampled among registered out-patients on rheumatoid arthritis clinic of Anam Hospital, Korea University Medical Center. The programmed experimental resistance exercises were carried out by the experimental group five times a week for eight weeks. Before and after experiments, level of functional task performance, functional disability, joint mobility, ESR, CRP, self-efficacy and family support were measured and statistically analysed. The results of the analysis of the effects on the Programmed Resistance Exercise are as follows : 1. A statistically significant post-test effects on functional task performance were revealed : 1) the experimental group climbed up and down the 10 steps of the stairs significantly faster than the control group(P=0.0001). 2) the experimental group walked 100 m significantly faster than the control group (P=0.0000). 3) After the experiment, the experimental group could sit down and get up from chairs with no armrest as low as 30cm, 40cm, 50cm on their on, with no assistance as opposed to the control group who could not sit down and get up from such low chairs (P=0.0084). 4) the experimental group lifted 0-15 kg object up to their own heights with no specific strain than the control group (P=0.0000). 2. A significant reduction in the functional disabilities was revealed in the experimental group(P=0.0017). 3. A notable incresement of the joint mobility was revealed in the experimental group. 4. A decrease in the ESR in both the experimental and the control groups was noticed, however, the difference was not statistically significant (P=0.9546). 5. The CRP of both the experimental and control groups decreased with no significant difference (P=0.6022). 6. The self-efficacy increased significantly in the experimental group(P=0.0042), however, no significant effect of self-efficacy on the actual practice of the program was noticed. 7. The family support revealed to be significantly higher in the experimental group (P=0.0013), however, the effect of the family support on actual practice of the program revealed not significant. Judging from the results of these experiments, the resistance exercise program not only improves the functional capacity of rheumatoid arthritis, but also diminishes their functional disabilities and has a great influence on increasing their joint mobility, self-efficacy, and family support. Concluding, in promoting the daily performance of rheumatoid arthritis, the resistance exercise program would be an appropriate nursing intervention.
Lee, Chang Soo;Park, Jong Hyok;Jung, Bong Jo;Choi, Young Jun
KSCE Journal of Civil and Environmental Engineering Research
/
v.29
no.4A
/
pp.385-393
/
2009
This study grasped the relationship between relative humidity in concrete and concrete shrinkage followed by pre-absorbed water of porous lightweight aggregates through measurements of concrete shrinkage and humidity and comparisons with established research results. It was showed that shrinkage reduction effect of lightweight concrete is 36% at 7 days early ages and 25% at 180 days long-term ages when water-binder ratio is 0.3. It also showed that shrinkage reduction effect is 19% at 7 days and 16% at 180 days when water-binder ratio is 0.4 and 37%, 32% when water-binder ratio is 0.5. The moisture supply effect of lightweight aggregates was remarkable at early age within 7~10 days irrespective of water-binder ratio. In case of waterbinder ratio is 0.3, the relationship between shrinkage and internal humidity of concrete has been underestimated regardless of applied existing model type and in case of water-binder ratio is 0.4, 0.5, measurement values are relatively similar with existing model equations. Finally this study did regression analyses about the relation among the humidity change and the shrinkage strain as a high-degree polynomial and derived parameters that can connect moisture movement analysis with differential shrinkage analysis in case of considering relative humidity at the time by moisture movement analysis of concrete.
Tensile constitutive stress-strain model of steel fiber reinforced concrete (SFRC) in fib MC2010 was investigated. In order to model tensile behavior of SFRC, three point loading flexural tests were conducted on notched small beams according to BE-EN-14651. Design parameters for the constitutive model were determined from the flexural tests. Flexural test and finite element analysis were conducted on large SFRC beam without steel reinforcements and compared with each other. In addition, parametric study on the effect of compressive and tensile model, and characteristic length on flexural behavior of the SFRC beam was conducted also. In results, pre-peak load-displacement curves from the FE analysis was close to experimental curves but significant difference was shown in post-peak behavior. The reason of the difference is originated from the fact that the fiber distribution and orientation were not being properly considered in the MC2010 model. This study shows that modification and detail explanations on the orientation factor K in MC2010 might require to better reproduce the behaviour of large scale SFRC beams.
Journal of the Korean Society for Marine Environment & Energy
/
v.8
no.4
/
pp.179-185
/
2005
Polycyclic aromatic hydrocarbons (PAHs) are a kind of toxic environmental pollutants and has been accumulated usually in marine sediments. Due to their potential hazardous to human, removal of PAHs from environments has been great concern. In the present study, the effect of microbial inoculation and the supplementation of mixed form cyclodextrin (M-CD) was assessed in the pre-sterilized or nonsterilized microcosms for optimizing operational conditions for ex situ bioremediation of sediments contaminated by PAHs. Activity of electron transport system (ETSA) was increased by the addition of M-CD regardless of inoculation of microorganisms in microcosms without sterilization. The degradation rate of PAHs in sterilized microcosms was app. 9-20% by the inoculation of single strain and 24-37% by the inoculation of microbial consortium supplemented with 1% M-CD, respectively. The degradation was not observed in microcosms without sterilization under the same conditions. The proportion of inoculated microorganisms also decreased in nonsterilized microcosms. Signals of inoculated bacteria were decreased to detection limit after 2 days in the microcosms without M-CD. In conclusion, microbial inoculation with appropriate carbon sources and removal of natural flora and grazers are required for the efficient ex situ bioremediation of sediments contaminated by PAHs in bioslurry reactor.
Mohammad Saeed, Amini;Vahab, Sarfarazi;Kaveh, Asgari;Xiao, Wang;Mojtaba Moheb, Hoori
Steel and Composite Structures
/
v.46
no.1
/
pp.53-73
/
2023
Man-made structure materials like concrete usually contain inclusions. These inclusions affect the mechanical properties of concrete. In this investigation, the influence of inclusion length and inclination angle on three-dimensional failure mechanism of concrete under uniaxial compression were performed using experimental test and numerical simulation. Approach of acoustic emission were jointly used to analyze the damage and fracture process. Besides, by combining the stress-strain behavior, quantitative determination of the thresholds of crack stress were done. concrete specimens with dimensions of 120 mm × 150 mm × 100 mm were provided. One and two holes filled by gypsum are incorporated in concrete samples. To build the inclusion, firstly cylinder steel tube was pre-inserting into the concrete and removing them after the initial hardening of the specimen. Secondly, the gypsum was poured into the holes. Tensile strengths of concrete and gypsum were 2.45 MPa and 1.5 MPa, respectively. The angle bertween inclusions and axial loadind ary from 0 to 90 with increases of 30. The length of inclusion vary from 25 mm to 100 mm with increases of 25 mm. Diameter of the hole was 20 mm. Entirely 20 various models were examined under uniaxial test. Simultaneous with experimental tests, numerical simulation (Particle flow code in two dimension) were carried out on the numerical models containing the inclusions. The numerical model were calibrated firstly by experimental outputs and then failure behavior of models containing inclusions have been investigated. The angle bertween inclusions and axial loadind vary from 0 to 90 with increases of 15. The length of inclusion vary from 25 mm to 100 mm with increases of 25 mm. Entirely 32 various models were examined under uniaxial test. Loading rate was 0.05 mm/sec. The results indicated that when inclusion has occupied 100% of sample thickness, two tensile cracks originated from boundaries of sample and spread parallel to the loading direction until being integrated together. When inclusion has occupied 75% of sample thickness, four tensile cracks originated from boundaries of sample and spread parallel to the loading direction until being integrated together. When inclusions have occupied 50% and 25% of sample thickness, four tensile cracks originated from boundaries of sample and spread parallel to the loading direction until being integrated together. Also the inclusion was failed by one tensile crack. The compressive strength of samples decease with the decreases of the inclusions length, and inclusion angle had some effects on that. Failure of concrete is mostly due to the tensile crack. The behavior of crack, was affected by the inclusion length and inclusion number.
Proceedings of the Korean Environmental Sciences Society Conference
/
1998.10a
/
pp.2-4
/
1998
Proliferation of Nocardia amarae cells in activated sludge has often been associated with the generation of nuisance foams. Despite intense research activities in recent years to examine the causes and control of Nocardia foaming in activated sludge, the foaming continued to persist throughout the activated sludge treatment plants in United States. In addition to causing various operational problems to treatment processes, the presence of Nocardia may have secondary effects on the fate of heavy metals that are not well known. For example, for treatment plants facing more stringent metal removal requirements, potential metal removal by Nocardia cells in foaming activated sludge would be a welcome secondary effect. In contrast, with new viosolid disposal regulations in place (Code o( Federal Regulation No. 503), higher concentration of metals in biosolids from foaming activated sludge could create management problems. The goal of this research was to investigate the metal sorption property of Nocardia amarae cells grown in batch reactors and in chemostat reactors. Specific surface area and metal sorption characteristics of N. amarae cells harvested at various growth stages were compared. Three metals examined in this study were copper, cadmium and nickel. Nocardia amarae strain (SRWTP isolate) used in this study was obtained from the University of California at Berkeley. The pure culture was grown in 4L batch reactor containing mineral salt medium with sodium acetate as the sole carbon source. In order to quantify the sorption of heavy metal ions to N amarae cell surfaces, cells from the batch reactor were harvested, washed, and suspended in 30mL centrifuge tubes. Metal sorption studies were conducted at pH 7.0 and ionlc strength of 10-2M. The sorption Isotherm showed that the cells harvested from the stationary and endogenous growth phase exhibited significantly higher metal sorption capacity than the cells from the exponential phase. The sequence of preferential uptake of metals by N. amarae cells was Cu>Cd>Ni. The specific surFace area of Nocardia cells was determined by a dye adsorption method. N.amarae cells growing at ewponential phase had significantly less specific surface area than that of stationary phase, indicating that the lower metal sorption capacity of Nocardia cells growing at exponential phase may be due to the lower specific surface area. The growth conditions of Nocardia cells in continuous culture affect their cell surface properties, thereby governing the adsorption capacity of heavy metal. The comparison of dye sorption isotherms for Nocardia cells growing at various growth rates revealed that the cell surface area increased with increasing sludge age, indicating that the cell surface area is highly dependent on the steady-state growth rate. The highest specific surface area of 199m21g was obtained from N.amarae cell harvested at 0.33 day-1 of growth rate. This result suggests that growth condition not only alters the structure of Nocardia cell wall but also affects the surface area, thus yielding more binding sites of metal removal. After reaching the steady-state condition at dilution rate, metal adsorption isotherms were used to determine the equilibrium distributions of metals between aqueous and Nocardia cell surfaces. The metal sorption capacity of Nocardia biomass harvested from 0.33 day-1 of growth rate was significantly higher than that of cells harvested from 0.5- and 1-day-1 operation, indicatng that N.amarae cells with a lower growth rate have higher sorpion capacity. This result was in close agreement with the trend observed from the batch study. To evaluate the effect of Nocardia cells on the metal binding capacity of activated sludge, specific surface area and metal sorption capacity of the mixture of Nocardia pure cultures and activated sludge biomass were determined by a series of batch experiments. The higher levels of Nocardia cells in the Nocardia-activated sludge samples resulted in the higher specific surface area, explaining the higher metal sorption sites by the mixed luquor samples containing greater amounts on Nocardia cells. The effect of Nocardia cells on the metal sorption capacity of activated sludge was evaluated by spiking an activated sludge sample with various amounts of pre culture Nocardia cells. The results of the Langmuir isotherm model fitted to the metal sorption by various mixtures of Nocardia and activated sludge indicated that the mixture containing higher Nocardia levels had higher metal adsorption capacity than the mixture containing lower Nocardia levels. At Nocardia levels above 100mg/g VSS, the metal sorption capacity of activate sludge increased proportionally with the amount of Noeardia cells present in the mixed liquor, indicating that the presence of Nocardia may increase the viosorption capacity of activated sludge.
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