• The Journal of the Convergence on Culture Technology
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• v.7 no.4
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• pp.391-396
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• 2021

The purpose of this study is to study the possibilities and limitations of arts education by utilizing SKT's ifland metaverse Platform for arts majors. To summarize the performance of the study, it is as follows. The subjects of the study were 50 students(25 experimental group, 25 control group) in Performing Arts, Entertainment and Music department of D University located at Gyeonggi, Korea. As a result of analyzing the difference between the pre-test and the post-test between the experimental group and the control group, it was found that there was a statistically significant effect on the effectiveness of the arts education method using the metaverse of the control group. It is hoped that the results of this study will be a case study that cannot be overlooked in the development of the today's arts educational medium as well as the virtual world in the field of arts and in cultural technology research, arts education is no longer a latecomer, but a leading pioneer and main agent, and I hope that it will become a new perspective for various studies connected with industry like the WAVE.

• Journal of Mushroom
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• v.20 no.4
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• pp.263-269
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• 2022

This study investigated the culture characteristics of Cryptoporus volvatus, whichis grow naturally in Korea, to determine the suitable environmental conditions for its cultivation. The physiological characteristics of the mycelia were assessed according to the cultivation conditions, to determine the optimal conditions for artificial cultivation. The visual characteristics of the hyphae of Cryptoporus volvatus KACC52303 included an irregular and uneven surface and a fuzzy or cotton-like texture. Under the microscope, its microstructure showed pre-chlamydospore formation, but no clamps were seen. The appropriate culture temperature was found to be a medium/high temperature of approximately 25-30℃, and the optimal pH was found to have a wide range from weakly acidic (pH 4) to neutral (pH 7). In the optimal nutrient source experiment, hyphal growth was shown to be fair in a mixed medium with 2.5% dextrin as the carbon source and 0.1% yeast extract as the organic nitrogen source. Among the various amino acids, organic acids, and inorganic salts tested, the fastest hyphal growth was observed in the presence of leucine, acetic acid or gluconic acid, and KCl or KH₂PO₄, respectively. The column test showed that the best mycelial growth occurred in a mixed medium of 80% pine sawdust, 10% rice bran, and 10% corncob sawdust.

• Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
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• v.8 no.6
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• pp.121-130
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• 2018

The purpose of this study is to analyze the effect of partnership type (pre-competitive, competitive, pro - competitive, non - competitive partnership) and national cultural characteristics on partnership performance. In order to analyze this, we conducted questionnaires on companies that have conducted international management and affiliated companies. A total of 223 questionnaires were sent. A total of 121 inquiries were collected from 224 survey requests. Based on this, a structured questionnaire was sent back to the staff members listed in the survey request, and a total of 98 samples were collected. The results of the empirical analysis through the questionnaire showed that the difference between the affiliation type and the national culture had a significant effect on the partnership performance. The results showed that the difference between the affiliation type and the national culture had a significant effect on the partnership performance. This could be a very meaningful implication for companies that are able to achieve successful alliances in a way that enables them to achieve successful alliances.

• (The) Research of the performance art and culture
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• no.38
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• pp.297-347
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• 2019

Jie Jie Hao Yin 『절절호음(節節好音)』 is a script collection produced during the Qianlong's ruling Period which had one of the most flourished period of Qing Dynasty's Palace Drama. This script collection is consisted of 86 scripts of 6 Festival Court Plays: New year's day (元旦), Lantern festival(上元), Yanjiu festival(燕九), Snow appreciation(賞雪), Day of Kitchen god worshipping(祀竈), New year's eve(除夕). These scripts are effective records to study the New Year's Eve Drama of Qianlong Period. Jie Jie Hao Yin carry out the performances not only through Kunqiang (崑腔), and Yiyang Tune (弋腔), but also through various types of entertaining performances such as dance, masque play, puppet show, and story telling. Most of the songs were sung by various gods praising the era of peace as the holiday approaches. In the contents of revealing the luck to be brought by the holidays, it sought for the colorful transformations of stages through adding humoristique short stories or various feast genres in between the performance. It also tries to seek for diversities of stages through active application of acrobatics. It also portrays folk customs from BaiYunGuan's temple fair, Yanjiu Festival in YanBin, the Lantern Fair of Lantern Festival, and various activities of wet markets from New Year's Eve from diverse points of views. Especially through various shows and Chinese folk-art forms, the popular folk activities and entertainments near Beijing in the beginning era of Qing Dynasty can be observed. Jie Jie Hao Yin is a very important resource to look at the popular art activities of Beijing during Qianlong Period, and how the Palace culture accepted the folk cultures and applied to its own advancements.

• Journal of Embryo Transfer
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• v.19 no.3
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• pp.191-199
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• 2004

This study was conducted to examine in vitro development of porcine embryos constructed by the microinjection of cultured fetal fibroblast cells into porcine oocytes matured in vitro. Single fetal donor cells were deposited into the perivitelline space of enucleated oocytes, followed by electrical fusion and activation. Activated embryos were cultured in NCSU-23 medium supplemented with 5％ FBS, at 38.5$^{\circ}C$ for 6 to 8 days in 5％ $CO_2$ and air. In experiment 1, fusion rates of nuclear transfer embryos did not differ for fetal fibroblast cells incubated in 5％ FBS + NCSU-23 or 5％ FBS + TL Heaps medium, nor did fusion rates of donor cells differ between 1-8 hr incubation durations. Fusion rates for the four treatment subclasses ranged from 72.1％ to 78.0％. In experiment 2, Pre-synchronization in medium containing 0.1 $\mu\textrm{g}$/m Hoechst 33342 an increase from 0 and 8 versus 15 h culture an increased percentage of porcine fibroblast cells in G2/M at the end of the synchronization period (12.4％, 17.5％ and 47.6％). Neither an increase in the concentration of H 33342 (0.2-1.6 $\mu\textrm{g}$/$m\ell$) nor a longer exposure time (12h, 18h and 24h) increased the proportion of porcine G2/M fibroblasts. In experiment 3, fusion rates did not differ significantly far nuclear transfer embryos constructed using donor cells cultured in 5％ FBS + NCSU-23 medium for 1-2, 6-8 or 12-14 days (60.0％, 73.3％ and 62.5％), respectively. The cleavage rate for nuclear transplant embryos using fetal fibroblast cells cultured for 1-2 days was 44.0％, significantly less than 56.7％ and 50.0％. for 6-8 or 12-14 days duration of culture, respectively. In experiment 4, the proportions of nuclear transfer embryos that developed to the $\geq$2 cell and to the blastocyst stage were not affected significantly by culture medium (5％ FBS + NCSU-23 or 5％ FBS + TL-Heaps) or by $O_2$ concentration of the culture (5％ vs 10％). Rates of development to the $\geq$2 cell stage ranged from 65.9％ to 70.1％, and development rates to the blastocyst stage ranged from 9.8％ to 12.5％ for the four treatment subclasses. Developmental rate was highest for embryos cultured in 5％ FBS + NCSU-23 under a gas atmosphere of 5％ $O_2$ in air.

• Research in Plant Disease
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• v.18 no.4
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• pp.331-337
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• 2012

Citrus melanose is one of important disease in citrus cultivation, reducing quality of citrus fruits and resulting in economic loss. Like other diseases in citrus, melanose was mainly protected by chemical fungicide in the field. Recently, alternative method of disease control is highly required due to the side effect of the chemicals. In this study four rhizobacterial strains TRH423-3, MRL408-3, THJ609-3, and TRH415-2 are selected by dual-culture testing its antifungal activity against Diaporthe citri causing citrus melanose. To investigate the protection efficacy of the selected rhizobacterial strains to citrus melanose, the bacteria were pre-treated on citrus leaves following inoculation with melanose pathogen. Pre-treatment with all selected rhizobacterial strains showed disease suppression in which the levels of protection rates were different by the rhizobacterial strains. Additional treatment with the rhizobacterial strains after the pathogen inoculation enhanced protection rates in all cases. The strain MRL408-3 and TRH423-3 were identified as Burkholderia gladioli, TRH415-2 as Pseudomons fluorescens and THJ609-3 as Pseudomonas pudia as a result of analyzing the internal transcript spaces of the rhizobacterial strains rDNA. The selected rhizobacterial strains may be valuable as biological control agents in the environment-friendly citrus farm in which chemical application is limited.

• Korean Journal of Food Science and Technology
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• v.44 no.5
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• pp.634-637
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• 2012

In strawberry production, among others, the high incidence of diseases by pathogenic fungi resulting in the reduction of fruit yield and quality requires the development of eco-friendly management systems rather than chemical sprays to control them. The diameter of colonies grown in media at $25^{\circ}C$ for 5 days was measured to evaluate the in vitro inhibition of mycelial growth of 5 pathogenic fungi by irradiation with ultraviolet (UV-C, 264 nm). The mycelial growth of 5 pathogenic fungi was inhibited in potato dextrose agar (PDA) by the irradiation of UV-C for 1 hour a day, and was dramatically inhibited by the irradiation of UV-C for 9-12 h a day. The irradiation of UV-C for 9-12 h a day inhibited completely the growth of the late blight pathogen, Phytophthora cactorum. The irradiation distance of 40 to 50 cm was effective for the inhibition of mycelial growth of fungi. The mycelial growth of fungi without pre-incubation was inhibited strongly by UV-C irradiation compared to fungi pre-incubated for 2 days without light. The mycelia growth of Colletotrichum gloeosprioides and Fusarium oxysporum was inhibited strongly by UV-C irradiation in vegetable 8 juice agar compared to PDA.

• The Journal of Korean Society for School & Community Health Education
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• v.3
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• pp.15-33
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• 2002

Researchers' common findings is that there are positive or negative effect of alcohol expectancy on drinking behavior. Therefore we would effectively prevent troublesome drinking of the youth and university students by inquiring and controlling critical factors affecting alcohol expectancy. The purposes of this thesis are, first, to empirically test factors affecting the alcohol expectancy level of elementary schoolchild(potential drinker).; second, to suggest the necessity for development of pre-alcohol prevention programs. On the basis of previous research, eighteen factors included in four categories(general characters, environmental characters, alcohol knowledge, drinking experience) affecting alcohol expectancy level were found out. 623 subjects used in this study were drawn from 8 elementary schools in Daegu, Korea. The empirical results suggested that the alcohol expectancy level of elementary schoolchildren was negative in general. And it was proved that 9 factors were significantly correlated with alcohol expectancy level. To put it concretely(see Fig.), (1) It was proved that schoolchildren with bad environment(live in oneself, displeased drinking feeling) rather than good environment(live with parents, nice drinking feeling) for drinking had more negative alcohol expectancy. (2) Korean traditional culture that partakes of sacrificial food and drink have an influence on the first drinking of most elementary schoolchildren. And it was proved that schoolchildren with this drinking experience rather than any other motives had less negative alcohol expectancy. (3) It was proved that schoolchildren adapting themselves rather than being difficult in school life had more negative alcohol expectancy. And the more knowledge about alcohol or drinking schoolchildren had, the more they had negative alcohol expectancy (4) It was proved that schoolchildren having drinking experience or drinking at present rather than having no drinking experience or not-drinking at present had less negative alcohol expectancy. (5) It was proved that schoolchildren having strong drinking intention rather than having weak or no drinking intention in the future had more positive alcohol expectancy. Based on previous results, guideline for development of pre-alcohol prevention programs can be represented: discriminated programs development on educatee, drinking education programs development increasing the power of self-control about alcohol and drinking, social education or continuing education programs development on drinking, open preschool education to substantially prevent drinking or alcoholism etc. The findings, however, should be interpreted with caution, because this study has several limitations in measurement and sampling as follows. First, selection bias because of limited selection of sampling. It is because the subjects are drawn from only 8 elementary schools in Daegu. Second, less refined measurement ; Therefore, it is necessary to develop more detailed measures on alcohol knowledge, alcohol expectancy level especially. Further researches should be suggested and encouraged with more refined methodologies.

• Journal of Korean Ophthalmic Optics Society
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• v.18 no.4
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• pp.525-531
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• 2013

Purpose: To identify the apoptosis caused by Proparacaine hydrochloride (PPC), a topical anesthesia, applied to conjunctival cell lines and determine whether pigallocatechin-gallate (EGCG), has protective effects on. Methods: The conjunctival cell lines were treated with 0.5% of Alcaine$^{(R)}$, 0.5% of PPC and 0.01% of Benzalkonium chloride (BAC) for 15 minutes, respectively in order to investigate the effects of topical anesthesia on cells, and followed by cultured for 12 and 24 hours. The recovery effects were investigated by measuring level of cellular proliferation inhibiting using MTT assay and LDH assay. The conjunctival cell lines were pre-treated with EGCG $10{\mu}M$ for 3 hrs and post-treated with 0.5% PPC for 15 mins in order to investigate whether EGCG has protective effects, flow cytometry were performed in order to observe apoptosis. Results: A result of the additional culture of 12 and 24 hours and again immediately after the treatment for 15 minutes 0.5% of Alcaine$^{(R)}$, 0.5% of PPC, the 0.01% of BAC, cell viability was not increased in all groups (p<0.05). The cell viabilities were higher than in cells 3 hours post-treated with $10{\mu}M$ of EGCG and pre-treated PPC 0.5% (68.2%), compared to cells ($32.2{\pm}2.0%$) treated only with 0.5% of PPC. PPC 0.5% also induced apoptosis in the treated group was reduced by the addition of EGCG. Conclusions: It is considered that the EGCG has cell protective effects when it is added to PPC, a topical anesthesia, by improving cell viability and inhibiting apoptosis.

• Korean Journal of Plant Resources
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• v.26 no.1
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• pp.75-83
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• 2013

The present study was examined seed germination and growth characteristics of Cynanchum wilfordii under the following conditions such as soaking temperature, concentrations of $GA_3$, trays and soil types, and shading conditions. In seed pre-treatment, germination rate was the highest at 93% when soaked in 100 ppm $GA_3$ at $20^{\circ}C$ and seedling growth was best in 50 plug cell tray. Physiological quality was best in the condition in which seeds were treated with 10 ppm $GA_3$ at $20^{\circ}C$. Taking into consideration the seedling growth, their physiological quality as well as economical aspects, seedlings with plant height (over 10 cm) and root length (over 10 cm) were grown vigorously 30-45 days after the seeds were sown in mid-April in TKS, TKS+perlite and TKS+rice hull of 128 or 200 plug cell tray.