• Journal of Korean Academy of Nursing Administration
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• v.12 no.3
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• pp.373-384
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• 2006

Purpose: This is a pilot study to identify patient safety risk factors and strategies for patient safety management perceived by nurses. Methods: Data were collected and analyzed with an open questionnaire from April to May 2005, targeted on 100 nurses working in two hospitals. The issues were 'what are risk factors for patients, nurses, and other medical practitioners? How do they prevent with the aftermath of risk factors, causes of incidents?' For data analysis, types and frequency of risk factors were worked out, using the Australian Incident Monitoring System Taxonomy. Results: The types of patient safety risk factor perceived by nurses were as follows ; therapeutic devices or equipment, infrastructure and services (29.5%), nosocomial infections (16.3%), clinical processes or procedures (15.4%), behavior, human performance, violence, aggression, security and safety (12.2%), therapeutic agents (9.7%), injuries and pressure ulcers (8.7%), logistics, organization, documentation, and infrastructure technology (5.6%). Strategies for patient safety included training of prevention of infection, education about safety management for patients and medical professionals, establishment of reporting system, culture of care, pre-elimination of risk factors, cooperative system among employees, and sharing information. Conclusion: These results will be used to provide evidences for patient safety management and educational program.

• Journal of Korean Academy of Nursing
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• v.15 no.2
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• pp.16-24
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• 1985

This study considered the problem of whether the method of Lamaze education changes maternal attitudes toward childrearing, material-infant interaction and is feasible for Korean culture in nineteen mother. infant dyads. Among those mothers, nine were assigned experimental group who received Lamaze education at the period of 7th and 8th month of gestation, and ten were assigned control group who received obstetric routine care. Maternal attitudes were assessed with selected items from Cohler's Maternal Atttiude Scale. Maternal Play Interaction Scale was used during play session to evaluate maternal sensitivity, infant response and mother-infant dyadic synchrony. There were no significant differences not only between the two groups, but also between the period of pre-lamaze education and post-delivery on the Cohler's maternal attitude scale. Experimental mothers and babies scored significantly higher on maternal sensitivity and infant response. But no differences in mother-infant, dyadic synchrony were found. This findings mean that the effect of the lamaza education on the maternal attitudinal change related to be needed the change of cognitive structure is delayed while the effect of that on the maternal infant behavioral response showed immediatly. Modification of Lamaze method is necessary for practical use in our sociocultural system.

• Journal of the Korean Institute of Landscape Architecture
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• v.24 no.3
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• pp.79-95
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• 1996

The traditional gardens of China were constructed on the basis of the common aesthetic consciousness between designers and users. As designers and users communicated each other through the medium of garden, they give suggestions to our design and appreciation of modern landscape architecture. The traditional gardens of China pursued to reach the state of Yi Jing(意境), and this state formed the keynote of the whole field of Chinese culture. Yi Jing is the aesthetic theory originated in Pre-Qin Era, and established in Tang-Dynasty. After this, this theory become the very important aesthetic category of Chinese aesthetics. Yi Jing is the process from conception to appreciation, and requires the three parts of designer, a work of art, and appreciator. To reach Yi Jing, designers must be well grounded and persevere in their efforts. They also had to have the ability of corresponding the inner order of environment and landscape, and expressing their own feelings and emotions into gardens. So ultimately, they were in pursuit of constructing the gardens as if something naturally created. The garden itself is the meeting place of designers and users. The space in which users can think of life, nature, history, and cosmos. In order to do this, designers design the real landscape and non-visual landscape. This design can give appreciators more fertile imagination. Appreciation perfects the Yi Jing of gardens. Yi Jing is created by co-work of artist and appreciator with common aesthetic consciousness and sense. Therefore, it is subjective, and it may be vary with man and time.

• Microbiology and Biotechnology Letters
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• v.20 no.2
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• pp.203-206
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• 1992

Conditions of fermentation for the production of demethyltetracycline were examined using the mutant, which was obtained through the cell fusion of demeclocycline producing strains, The optimum temperature and the initial pH of broth for demethyltetracycline fermentation were $25^{\circ}C$ and 6.7, respectively. Unlike any other cases, the control of pH with alkali solution during the fermentation process affected the productivity. As a general rule, the larger the inoculum size the higher the early consumption of sugar and the viscosity of broth, which means that fermentation proceeds more rapidly as the inoculum size is increased. The highest productivity was shown when the inoculum size was 5% (v/v), and the phase of seed also influenced the fermentation. Among the parameters of pre-culture thus examined, pH was the most important factor.

• Health Policy and Management
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• v.8 no.2
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• pp.88-109
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• 1998

The main purpose of this study is to compare the traditional cost system and ABC(Activity Based Cost) system of clinical laboratory department in a hospital. The study subject was 296 services in clinical laboratory from March, 1997 to August, 1997. In a new costing system, cost for a lab test consist of direct cost element, activity based cost element, and allocated common cost element. In a traditional cost system, cost elements included direct cost element and indirect cost allocated based on test volumes The major findings of this research were as follows. 1. In the application of ABC system, total cost was analyzed as follows. Direct cost was 39.3% of total cost. Activity cost and allocation were 20.9% and 39.8%, respectively. The results of analysis to use traditional cost system were as follows. Direct cost was 39.3% and it was as same as the result of direct cost of ABC system. Indirect cost was 60.7%. 2. Activities of clinical laboratory of subject hospital were registration, pre-test operation, test, test result handling, delivery, culture, post-test operation, technical support, management support, and educational support. 3. The differences of the case of higher number of test case being carried out, the cost of ABC system was lower than the cost of traditional cost system. Otherwise in the case of lower number of test case being carried out, the rests have not been appropriately evaluated, and effective management were needed in clinical laboratory.

• Archives of Pharmacal Research
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• v.21 no.5
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• pp.521-526
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• 1998

The physicochmical properties of recombinant hepatitis B surface antigen (r-HBsAg), which was expressed in C127 mammalian cell were studied. Using roller bottle culture in DMEM supplemented with fetal bovine serum, 10-15 mg/L of r-HBsAg was produced with about 31% of purification yield. The purity of r-HBsAg by HPLC was 99.8% and electron microscopic examination showed homogeneous spherical particle with 22 nm in diameter, a morphological characteristic of HBsAg. The density of r-HBsAg by CsCI density gradient method was 1.19g/ml and the isoelectric point by Mono $P^{TM}$ HR 5/20 column was 4.6. The analysis of subunit protein pattern using SDS-PAGE followed by scanning densitometry gave 81.3% of S protein and 18.7% of pre-S protein. fluorophore-assisted-carbohydrate-electrophoresis analysis showed the relative amount of carbohydrate to protein was 1.7% and it smajr component was N-acetyl glucosamine, which was about 39% of total carbohydrate. The relative amount of lipid to protein determined by vanillin phosphoric acid method was 32.5% and its major component was phospholipid, which was about 70% of total lipid. The physicochemical properties of C127 mammalian cell-derved r-HBsAg are similar to those of p-HBsAg, suggesting that the r-HBsAg can be used in developing a new preventive vaccine against hepatitis B.

• Journal of the Korean Society of Clothing and Textiles
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• v.14 no.1 s.33
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• pp.55-67
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• 1990

This is a review on the longitudinal transformation of the Japanese trousers, which is prepared as a pre-study for the comparative analysis of the forms of Korean and Japanese folk trousers. The special traits of the Japanese trousers are found to be as follows: 1) The Japanese trousers seem to have their root in the Northern-Asian or Schithian culture. 2) While the ancient forms of Japanese trousers, esp., those of the 8C before include the same types as Korean folk trousers, the Medieval and Recent era trousers quite differ and transform themselves into the unique Japanese type. 3) All Japanese trousers can largely be classified into formal and functional trousers. Formal trousers play important role as one of the ceremonial wardrobe and are treated rather as an outermost-wear than as an inner-wear. 4) The formal trousers have skirt-like features with much fullness as are presented in many deep pleats, long crotch lines and long sashes connected to the belt; the femine trousers even omit crotch line. 5) The principal forms of Japanese trousers consist of I, $\wedge,\;\wedge$; the $\lambda$ form of Korean and Chinese type had never existed. 6) Varieties of the composite type of Japanese-Western style appear among the trousers of recent time.

• Reproductive and Developmental Biology
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• v.29 no.4
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• pp.229-234
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• 2005

Methods for activation of reconstructed oocytes were examined for the production of nuclear transfer (NT) rat embryos using fetal neural stem cells as donor. Neural stem cells were isolated from Day 14.5 rat fetuses, and the oocytes for recipient cytoplasm were recovered from 4-week old Sprague Dawley rats. After enucleation and nuclear injection, the reconstructed oocytes were immediately exposed to activation medium consisting of 10 mM $SrCl_2$ for 4 h (immediate activation after injection; IAI), or cultured in vitro for $2\~3$ h before activation treatment (injection before activation; IBA). Pre-activated oocytes were also used for NT to test reprogramming potential of artificially activated oocytes. The oocytes were grouped as IIA (immediate injection after activation) and ABI (activation $2\~3$ h before injection). Following NT, the oocytes were cultured in vitro. Development of the NT embryos was monitored at 44 and 119 h after activation. The embryos in groups IAI, mA, and IIA were cleaved to the 2-cell stage at the rates of $36.6\%\;(15/41),\;39.5\%\;(17/43)\;and\;46.3\%$ (25/54), respectively. However, in the ABI group, only one embryo ($1.8\%$, 1/55) was cleaved after activation. After in vitro culture, two NT embryos from IAI group had developed to the morula stage $(4.9\%\cdot2/41)$. However, no morula or blastocyst was obtained in the other groups. These results suggest that immediate activation after injection (IAI) method may be used for the production of rat somatic cell NT embryos.

• Journal of Pharmaceutical Investigation
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• v.36 no.5
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• pp.309-314
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• 2006

The multicellular layers(MCL) of human cancer cells is a three dimensional(3D) in vitro model for human solid tumors which has been used primarily for the assessment of avascular penetration of anti-cancer drugs. For anti-cancer drugs with penetration problem, MCL represents a good experimental model that can provide clinically relevant data. Calcein-AM is a fluorescent dye that demonstrates the cellular vitality in a graded manner in cancer cell culture system. In the present study, we evaluated the use of calcein-AM for determination of anti-proliferative activity of anti-cancer agents in MCL model of DLD-1 human colorectal cancer cells. Optical sectioning of confocal imaging was compromised with photonic attenuation and penetration barrier in the deep layers of MCL. By contrast, fluorescent measurement on the cryo-sections provided a feasible alternative. Cold pre-incubation did not enhance the calcein-AM distribution to a significant degree in MCL of DLD-1 cells. However, the simultaneous determination of drug penetration and cellular vitality appeared to be possible in drug treated MCL. In conclusion, these data suggest that calcein-AM can be used for the simultaneous determination of drug-induced anti-proliferative effect and drug penetration in MCL model.

• Journal of Korean Public Health Nursing
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• v.14 no.2
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• pp.304-317
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• 2000

The purpose of this study is to identify the effect of the voluntary program experience with curriculum on self-esteem and depression in nursing college students. The research design utilized in this study was one group pre test-post test design. The data were gathered two times with questionnaire. First data were gathered before voluntary activity. And second data were gathered after instruction and five times voluntary activity. The data were analized by frequency, paired t-test. t-test. and ANOVA using the SAS program. The results were as follows 1. After the activity the scores of self-esteem were significantly higher than before the activity. 2. After the activity the scores of depression were significantly lower than before the activity. 3. The relationship between self-esteem and depression were negatives in both before and after the activity. 4. Before voluntary activity the scores of self-esteem in the class choosing the Culture Exploration were significantly high. but after the activity the scores of self-esteem were not significantly different. 5. As opinion on attendance of lecture class of voluntary activity program. before voluntary activity the scores of depression were not significantly different. but after the activity the scores of depression were significantly different.