• ALGAE
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• 제20권4호
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• pp.305-314
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• 2005

5 Haptophyta 2 Chrysophyta and 1 Prasinophyta species of scale-bearing nanoflagellates were collected in coastal water of Korea and identified by examination of their scales with Field Emission Scanning Electron Microscope (JSM-6700F). These included Chrysochromulina ahrengoti, C. simplex, C. spinifera, Prymnesium parvum, P. patelliferum, Mamiella gilva, Paraphysomonas imperforata and Pa. vestita. The surface of cells covered with unmineralised scales (5 Haptophyte and 1 Prasinophyta species) or silica scales (2 Chrysophyta species). Scale-covered flagella are found in the 1 Prasinophyta species. One of the main structural characteristics of Haptophyte is the haptonema, a filiform organelle which occurs together with the two flagella. It may be long and coiling upon irritation as in Chrysochromulina, or short and noncoiling as in Prymnesium.

• Fisheries and Aquatic Sciences
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• 제4권2호
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• pp.75-83
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• 2001

This study is to investigate biochemical compositions of two species of marine microalgae, Chlorella ellipsoidea of Chlorophyta and Tetraselmis suecica of Prasinophyta, and to assess their potential antimicrobial activities. Crude protein, lipid and carbohydrate for C. ellipsoidea were $43.15\%$, $12.63\%$ and $13.09\%$, respectively, and those for T. suecica were $44.95\%$, $4.80\%$ and $24.05\%$, respectively. The major amino acids of the two micro algae were aspartic acid, glutamic acid, glycine, alanine, valine, leucine, lysine and proline, and no significant difference between the amino acid compositions of both micro algae was observed. The major sugars for both microalgae were glucose, galactose and mannose, and glucose contents showed the highest level, $58.70\%$ for C. ellipsoidea and$57.86\%$ for T. suecica. The major mineral contents of both micro algae for 100g were Ca (3,114mg in C. ellipoidea and 3,389mg in T. suecica) and followed by Na (2,881mg), K (548mg) and Mg (545mg) for C. ellipsoidea and Na (1,832 mg), Mg (1,510mg) and K (548mg) for T. suecica. In the content of ATP-related compound, hypoxanthine in C. ellipsoidea and IMP in T. suecica were absolutely dominant compounds. The highest content of fatty acid in C. ellipsoidea was 20:4, $27.15\%$ and that in T. suecica was 18:3 (w-6), $18.10\%$. In case of physiologically important polyunsaturated fatty acids like eicosapentaenoic acid (20: 5) and docosahexaenoic acid (22: 6), both microalgae possessed just trace amounts but was rich in arachidonic acid (20: 4). Vitamin content in both microalgae was significantly high in choline and inositol. In antimicrobial activity by water- and fat-soluble fraction of the micro algae, hexane extract in the fat-soluble fraction of C. elliposidea inhibited the growth of Bacillus subtilis by $96\%$ bactericidal activity and tetrachlorocarbon extract of T. suecica indicated relatively excellent antimicrobial activity $(81\%\;bactericidal\;activity)$ against Escherichia coli. Hot water extract among water-soluble fraction of both micro algae almost suppressed the growth of Staphylococcus aureus by $96\%$ bactericidal activity.

• 한국수산과학회지
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• 제35권2호
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• pp.155-161
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• 2002

해양 미세조류는 해양생물의 영양원으로서 대부분 소비되고 있으며, 그 종류도 매우 다양하다. 이들 미세조류로부터 유용물질의 회수 및 이용은 미이용 수산자원의 효율적 이용과 아울러 생물 및 식량자원으로서 그리고 새로운 기능성 소재로서 활용될 수 있을 것이다. 본 연구에서는 이러한 해양 미세조류의 효율적인 이용을 위하여 비교적 성장이 빠르고 배양이 용이한 담녹조류인 T. suecica 중의 항산화성 물질을 분리정제하여 구조를 결정하였다. 미세조류 T. suecica의 유기용매 추출물 중의 항산화성은 chloroform 획분이 가장 좋았으며, 이 획분을 silica gel column chromatography, PTLC 및 HPLC를 사용하여 항산화성 물질을 분리${\cdot}$정제하였다. Silica gel column을 사용하여 분리된 획분의 항산화성은 hexane: ethylacetate (1:5)로 용출시킨 획분에서 가장 높았다. 이 획분은 PTLC에 전개하여 8개의 획분을 얻었으며, 그 중에서 Rf값 0.40인 획분에서 활성이 높게 나타났다 HPLC에서 단일 획분으로 정제된 물질의 항산화성은$IC_{50}$ 값으로 10.2$\mu$g/mL였으며, 화학적으로 합성된 항산화제인 BHT 및 BHA보다는 낮았지만 천연 항산화제인 $\alpha$-tocopherol과 비교될 수 있었다 이 물질의 화학적 구조는 chlorophyll을 전구체로 하는 pheophorbide-a로 동정되었으며, 항산화 활성은 a-tocopherol과 비교될 수 있어 식품 및 관련분야에서 항산화제로서 이용할 수 있을 것으로 기대된다.