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Analysis of Endophytic Bacterial Communities and Investigation of Core Taxa in Apple Trees

  • Yejin Lee;Gyeongjun Cho;Da-Ran Kim;Youn-Sig Kwak
    • The Plant Pathology Journal
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    • v.39 no.4
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    • pp.397-408
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    • 2023
  • Fire blight disease, caused by Erwinia amylovora, is a devastating affliction in apple cultivation worldwide. Chemical pesticides have exhibited limited effectiveness in controlling the disease, and biological control options for treating fruit trees are limited. Therefore, a relatively large-scale survey is necessary to develop microbial agents for apple trees. Here we collected healthy apple trees from across the country to identify common and core bacterial taxa. We analyzed the endophytic bacterial communities in leaves and twigs and discovered that the twig bacterial communities were more conserved than those in the leaves, regardless of the origin of the sample. This finding indicates that specific endophytic taxa are consistently present in healthy apple trees and may be involved in vital functions such as disease prevention and growth. Furthermore, we compared the community metabolite pathway expression rates of these endophyte communities with those of E. amylovora infected apple trees and discovered that the endophyte communities in healthy apple trees not only had similar community structures but also similar metabolite pathway expression rates. Additionally, Pseudomonas and Methylobacterium-Methylorobrum were the dominant taxa in all healthy apple trees. Our findings provide valuable insights into the potential roles of endophytes in healthy apple trees and inform the development of strategies for enhancing apple growth and resilience. Moreover, the similarity in cluster structure and pathway analysis between healthy orchards was mutually reinforcing, demonstrating the power of microbiome analysis as a tool for identifying factors that contribute to plant health.

A Multi-Level Digital Twin for Optimising Demand Response at the Local Level without Compromising the Well-being of Consumers

  • Byrne, Niall;Chassiakos, Athanassios;Karatzas, Stylianos;Sweeney, David;Lazari, Vassiliki;Karameros, Anastasios;Tardioli, Giovanni;Cabrera, Adalberto Guerra
    • International conference on construction engineering and project management
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    • 2022.06a
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    • pp.408-417
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    • 2022
  • Although traditionally perceived as being a visualization and asset management resource, the relatively rapid rate of improvement of computing power, coupled with the proliferation of cloud and edge computing and the IoT has seen the expanded functionality of modern Digital Twins (DTs). These technologies, when applied to buildings, are now providing users with the ability to analyse and predict their energy consumption, implement building controls and identify faults quickly and efficiently, while preserving acceptable comfort and well-being levels. Furthermore, when these building DTs are linked together to form a community DT, entirely new and novel energy management techniques, such as demand side management, demand response, flexibility and local energy markets can be unlocked and analysed in detail, creating circularity in the economy and making ordinary building occupants active participants in the energy market. Through the EU Horizon 2020 funded TwinERGY project, three different levels of DT (consumer - building - community) are being created to support the creation of local energy markets while optimising building performance for real-time occupant preferences and requirements for their building and community. The aim of this research work is to demonstrate the development of this new, interrelated, multi-level DT that can be used as a decision-making tool, helping to determine optimal scenarios simultaneously at consumer, building and community level, while enhancing and successfully supporting the community's management plan implementation.

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Factors Influencing Sleep Quality in Nursing Students - Focusing on Depression, Smartphone Usage before Sleep and Academic Score - (간호대학생 수면의 질 영향요인 - 우울, 수면 전 스마트폰 사용행태, 학업 성적을 중심으로 -)

  • Kim, Eun Ju
    • The Journal of the Korea Contents Association
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    • v.22 no.8
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    • pp.640-647
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    • 2022
  • The purpose of this study was to investigate the factors influencing sleep quality of nursing students, focusing on depression, smartphone usage before sleep, and academic score. Among nursing students in the 3rd and 4th grades of two universities, 196 subjects were randomly selected. Data were collected from April to July 2021 using tool of sleep quality(PSQI-K), depression(Korean BDI-II), and smartphone usage before sleep, and the effect on sleep quality was analyzed through Stepwise Multiple Regression Analysis. As a result of the study, two factors, depression and smartphone usage before sleep, had 39.0% explanatory power for the sleep quality of nursing students, and were statistically significant. That is, the higher the depression level and the more frequent smartphone usage before sleep, the worse the sleep quality level. However, academic score was not a significant variable. In conclusion, in order to improve the sleep quality of nursing students, it is necessary to reduce the pattern of sleep disorders related to depression and to correct the lifestyle by reducing smartphone usage before sleep.

Application of IPS model of city competitiveness to Korean cities, and strategies to enhance Chuncheon city's competitiveness (IPS 도시경쟁력 평가 모델과 한국 도시의 경쟁력 평가 결과, 그리고 춘천시의 경쟁력 향상 전략)

  • Cho, Dongsung;Im, Minyoung
    • Journal of Leisure Studies
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    • v.6 no.3
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    • pp.39-56
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    • 2009
  • With the main player of international competition being changed from country to region/city, city competitiveness is emerging as driving forces of industrial and national competitiveness and organizations formed on a city level are regarded as the main unit of competition among nations. This paper's goal is to research upon the city competitiveness with an emphasis on the analytical tool applying IPS Model of city competitiveness to 75 Korean cities and to map out the strategies to enhance the Chuncheon city's competitiveness based on the results of the evaluation on Chuncheon city's current and future competitiveness with focus on the impact of the city's hosting World Leisure Congress and World Leisure Games in 2010. By discussing city's future competitiveness as well as its current strength at the same time, the more comprehensive perspective required in reinforcing city competitiveness is proposed in this paper and issues related to a city's context and policy can be evaluated and their solutions can be sought after. The study can also contribute to the establishment of city's development policies and detect sustainable growth power for individual cities.

Utilization of UAV Remote Sensing in Small-scale Field Experiment : Case Study in Evaluation of Plat-based LAI for Sweetcorn Production

  • Hyunjin Jung;Rongling Ye;Yang Yi;Naoyuki Hashimoto;Shuhei Yamamoto;Koki Homma
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.75-75
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    • 2022
  • Traditional agriculture mostly focused on activity in the field, but current agriculture faces problems such as reduction of agricultural inputs, labor shortage and so on. Accordingly, traditional agricultural experiments generally considered the simple treatment effects, but current agricultural experiments need to consider the several and complicate treatment effects. To analyze such several and complicate treatment effects, data collection has the first priority. Remote sensing is a quite effective tool to collect information in agriculture, and recent easier availability of UAVs (Unmanned Aerial Vehicles) enhances the effectiveness. LAI (Leaf Area Index) is one of the most important information for evaluating the condition of crop growth. In this study, we utilized UAV with multispectral camera to evaluate plant-based LAI of sweetcorn in a small-scale field experiment and discussed the feasibility of a new experimental design to analyze the several and complicate treatment effects. The plant-based SR measured by UAV showed the highest correlation coefficient with LAI measured by a canopy analyzer in 2018 and 2019. Application of linear mix model showed that plant-based SR data had higher detection power due to its huge number of data although SR was inferior to evaluate LAI than the canopy analyzer. The distribution of plant-based data also statistically revealed the border effect in treatment plots in the traditional experimental design. These results suggest that remote sensing with UAVs has the advantage even in a small-scale experimental plot and has a possibility to provide a new experimental design if combined with various analytical applications such as plant size, shape, and color.

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Verification on stock return predictability of text in analyst reports (애널리스트 보고서 텍스트의 주가예측력에 대한 검증)

  • Young-Sun Lee;Akihiko Yamada;Cheol-Won Yang;Hohsuk Noh
    • The Korean Journal of Applied Statistics
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    • v.36 no.5
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    • pp.489-499
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    • 2023
  • As sharing of analyst reports became widely available, reports generated by analysts have become a useful tool to reduce difference in financial information between market participants. The quantitative information of analyst reports has been used in many ways to predict stock returns. However, there are relatively few domestic studies on the prediction power of text information in analyst reports to predict stock returns. We test stock return predictability of text in analyst reports by creating variables representing the TONE from the text. To overcome the limitation of the linear-model-assumption-based approach, we use the random-forest-based F-test.

Full-scale TBM excavation tests for rock-like materials with different uniaxial compressive strength

  • Gi-Jun Lee;Hee-Hwan Ryu;Gye-Chun Cho;Tae-Hyuk Kwon
    • Geomechanics and Engineering
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    • v.35 no.5
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    • pp.487-497
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    • 2023
  • Penetration rate (PR) and penetration depth (Pe) are crucial parameters for estimating the cost and time required in tunnel construction using tunnel boring machines (TBMs). This study focuses on investigating the impact of rock strength on PR and Pe through full-scale experiments. By conducting controlled tests on rock-like specimens, the study aims to understand the contributions of various ground parameters and machine-operating conditions to TBM excavation performance. An earth pressure balanced (EPB) TBM with a sectional diameter of 3.54 m was utilized in the experiments. The TBM excavated rocklike specimens with varying uniaxial compressive strength (UCS), while the thrust and cutterhead rotational speed were controlled. The results highlight the significance of the interplay between thrust, cutterhead speed, and rock strength (UCS) in determining Pe. In high UCS conditions exceeding 70 MPa, thrust plays a vital role in enhancing Pe as hard rock requires a greater thrust force for excavation. Conversely, in medium-to-low UCS conditions less than 50 MPa, thrust has a weak relationship with Pe, and Pe becomes directly proportional to the cutterhead rotational speed. Furthermore, a strong correlation was observed between Pe and cutterhead torque with a determination coefficient of 0.84. Based on these findings, a predictive model for Pe is proposed, incorporating thrust, TBM diameter, number of disc cutters, and UCS. This model offers a practical tool for estimating Pe in different excavation scenarios. The study presents unprecedented full-scale TBM excavation results, with well-controlled experiments, shedding light on the interplay between rock strength, TBM operational variables, and excavation performance. These insights are valuable for optimizing TBM excavation in grounds with varying strengths and operational conditions.

Effects of Clinical Nurses Critical Reflection Competency, Professional Pride, and Person-Centered Care Practice on Patient Safety Management Activities (임상간호사의 비판적 성찰 역량, 전문직 자부심, 인간중심간호 수행이 환자안전간호활동에 미치는 영향)

  • Lee, Subin;Shin, Sujin
    • Journal of Korean Critical Care Nursing
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    • v.16 no.3
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    • pp.87-98
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    • 2023
  • Purpose : This descriptive survey investigated the effects of clinical nurses' critical reflection competency, professional pride, and person-centered care practices on patient safety management activities. Methods : The participants were 183 clinical nurses working at a tertiary hospital in South Korea. The questionnaires consisted of the Critical Reflection Competency Scale for Clinical Nurses Professional Pride the Person-Centered Nursing Assessment Tool and Patient Safety Management Activities. The collected data were analyzed using descriptive statistics, t-tests, ANOVA, Tukey's HSD, Pearson's correlation coefficient, and multiple regression using SPSS 29.0. Results : The mean score for patient safety management activities was 4.65±0.34 out of 5. There were significant differences in patient safety management activities according to age (F=3.90, p =.010), education level (t=-2.56, p =.013), total work experience (F=3.87, p =.010), and the number of healthcare accreditation system experiences (F=5.22, p =.006). Patient safety management activities were positively correlated with critical reflection competency (r=.337, p <.001), professional pride (r=.271, p <.001), and person-centered care practices (r=.399, p <.001). The results indicated that person-centered care practices affected patient safety management activities (𝛽=.358, p <.001) with the explanatory power of 22.5%. Conclusion : To improve clinical nurses' patient safety management activities, it is necessary to develop participatory educational programs that can integrate skills and attitudes based on conceptual knowledge of person-centered care. Intervention studies are needed to test the effect of person-centered care on patient safety when applied in clinical practice.

Perception of the Neo-Confucian body in men's dress during the Joseon Dynasty (조선시대 남성복식에 발현된 성리학적 몸 인식)

  • Yoon Jung Ko ;Eunhyuk Yim
    • The Research Journal of the Costume Culture
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    • v.31 no.5
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    • pp.573-585
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    • 2023
  • Comprehending the prevailing ideals of the body within a specific era requires grasping the intricate interplay between social phenomena and the evolution of clothing. Accordingly, this study investigates the distinctive facets of the perception of the Neo-Confucian body as reflected in men's dress during the Joseon Dynasty. We examine a comprehensive body of scholarship, literature, and historical records concerning the body and dress. Additionally, we also employ a framework developed by M. Y. Kim, which categorizes the Neo-Confucian body in three ways: as the natural body, the cultural body, and the body as a fully-realized moral subject. Our findings unveil three crucial insights: firstly, guided by Neo-Confucian discourse positing appearance as a manifestation of innate energy (氣), men's dress was deliberately designed to demarcate stylistic distinctions in women's dress; secondly, the Chinese gwan (冠) was employed as a tool of self-cultivation (修身) to symbolize the legitimacy of Joseon's Neo-Confucian governance; and thirdly, sim-ui (深衣), a philosophical emblem of Confucianism extensively represented across through an intensified exploration of historical sources, served as a means to consolidate the political standing of the Neo-Confucian faction. As a consequence of these factors, the attire of noble men conferred upon them both sexual and moral ascendancy as political entities; men's dress became a visual manifestation of the legitimacy of their power, thus embodying Neo-Confucian ideals. This study carries significance by applying a discourse analysis approach to Korean dress research and elucidating the factors underlying the development of men's dress during the Joseon Dynasty.

Prediction of Draft Force of Moldboard Plow according to Travel Speed in Cohesive Soil using Discrete Element Method (이산요소법을 활용한 점성토 환경에서의 작업 속도에 따른 몰드보드 플라우 견인력 예측)

  • Bo Min Bae;Dae Wi Jung;Dong Hyung Ryu;Jang Hyeon An;Se O Choi;Yeon Soo Kim;Yong Joo Kim
    • Journal of Drive and Control
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    • v.20 no.4
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    • pp.71-79
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    • 2023
  • In the field of agricultural machinery, various on-field tests are conducted to measure design load for optimal design of agricultural equipment. However, field test procedures are costly and time-consuming, and there are many constraints on field soil conditions due to weather, so research on utilizing simulation to overcome these shortcomings is needed. Therefore, this study aimed to model agricultural soils using discrete element method (DEM) software. To simulate draft force, predictions are made according to travel speed and compared to field test results to validate the prediction accuracy. The measured soil properties are used for DEM modeling. In this study, the soil property measurement procedure was designed to measure the physical and mechanical properties. DEM soil model calibration was performed using a virtual vane shear test instead of the repose angle test. The DEM simulation results showed that the prediction accuracy of the draft force was within 4.8% (2.16~6.71%) when compared to the draft force measured by the field test. In addition, it was confirmed that the result was up to 72.51% more accurate than those obtained through theoretical methods for predicting draft force. This study provides useful information for the DEM soil modeling process that considers the working speed from the perspective of agricultural machinery research and it is expected to be utilized in agricultural machinery design research.