• 동의생리병리학회지
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• 제25권2호
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• pp.202-211
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• 2011

The stems and branchs of Euryale ferox Salisbury (EF), are used in Chinese herbal medicine for latent-heat-clearing, antipyretic, detoxicant and anti-inflammatory ailments. This plant is used worldwide for the treatment of many types of inflammatory disease including respiratory infections, diabetes mellitus, rheumatoid arthritis and play an important role in the immune reaction. Topical natural antioxidants are a useful strategy for the prevention of oxidative stress mediated inflammatory disease. Plants produce significant amounts of antioxidants to prevent the oxidative stress caused by photons and oxygen, therefore they represent a potential source of new compounds with antioxidant activity. This study was designed to evaluate whether EFEA (ethylacetate fraction of EF) may ameliorate oxidative stress and inflammatory status through the antioxidative mechanism in LPS-stimulated RAW 264.7 murine macrophage cell line. Treatment of RAW 264.7 cells with EFEA significantly reduced LPS-stimulated inflammatory response in a dose-dependent manner. In conclusion, the EF extracts have anti-inflammatory effects in vitro system, which can be used for developing pharmaceutical drug against oxidative stress and chronic inflammatory disease.

• 동의생리병리학회지
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• 제25권2호
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• pp.280-293
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• 2011

There is currently increased interest in the identification of antioxidant compounds that are pharmacologically potent and have low or no side effects. Plants produce significant amounts of antioxidants to prevent the oxidative stress caused by photons and oxygen, therefore they represent a potential source of new compounds with antioxidant activity. FARFARE FLOS has been frequently used on the respiratory system including bronchitis, phthisis. In this study, the antioxidant activity of extract from FF was studied in vitro methods by measuring the antioxidant activity by TEAC, measuring the scavenging effects on reactive oxygen species (ROS) [superoxide anion, hydroxyl radical] and on reactive nitrogen species (RNS) [nitric oxide and peroxynitrite] as well as measuring the inhibitory effect on Cu2+-induced human LDL oxidation. The FF extracts were found to have a potent scavenging activity, as well as an inhibitory effect on LDL oxidation. And this study was designed to evaluate whether FFEA may ameliorate oxidative stress and inflammatory status through the antioxidative mechanism in LPS-stimulated RAW 264.7 murine macrophage cell line. Treatment of RAW 264.7 cells with FFEA significantly reduced LPS-stimulated inflammatory response in a dose-dependent manner. In conclusion, the FF extracts have anti-oxidative and anti-inflammatory effects in vitro system, which can be used for developing pharmaceutical drug against oxidative stress and atherosclerosis.

• Parasites, Hosts and Diseases
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• 제41권4호
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• pp.243-245
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• 2003

Neospora caninum is an important cause of abortion in cattle, and dogs are its only known definitive host. Its seroprevalence among domestic urban and rural dogs and feral raccoon dogs (Nyctereutes procyonoides koreensis) in Korea was studied by indirect fluorescent antibody test (IFAT) and by the neospora agglutination test (NAT), respectively. Antibodies to N. caninum were found in 8.3% of urban dogs and in 21.6% of dogs at dairy farms. Antibody titers ranged from 1:50 to 1:400. Antibodies to N. caninum were found in six (23%) of 26 raccoon dogs. However, the potential role of raccoon dogs as a source of horizontal transmission of bovine neosporosis needs further investigation. The results of this study suggest that there is a close relationship between N. caninum infection among dairy farm dogs and cattle in Korea. This study reports for the first time upon the seroprevalence of N. caninum infection in raccoon dogs in Korea.

• Biomolecules & Therapeutics
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• 제28권5호
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• pp.414-422
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• 2020

Fangchinoline (FAN) is a bisbenzylisoquinoline alkaloid that is widely known for its anti-tumor properties. The goal of this study is to examine the effects of FAN on arthritis and the possible pathways it acts on. Human fibroblast-like synovial cells (FLS), carrageenan/kaolin arthritis rat model (C/K), and collagen-induced arthritis (CIA) mice model were used to establish the efficiency of FAN in arthritis. Human FLS cells were treated with FAN (1, 2.5, 5, 10 µM) 1 h before IL-1β (10 ng/mL) stimulation. Cell viability, reactive oxygen species measurement, and western blot analysis of inflammatory mediators and the MAPK and NF-κB pathways were performed. In the animal models, after induction of arthritis, the rodents were given 10 and 30 mg/kg of FAN orally 1 h before conducting behavioral experiments such as weight distribution ratio, knee thickness measurement, squeaking score, body weight measurement, paw volume measurement, and arthritis index measurement. Rodent knee joints were also analyzed histologically through H&E staining and safranin staining. FAN decreased the production of inflammatory cytokines and ROS in human FLS cells as well as the phosphorylation of the MAPK pathway and NF-κB pathway in human FLS cells. The behavioral parameters in the C/K rat model and CIA mouse model and inflammatory signs in the histological analysis were found to be ameliorated in FAN-treated groups. Cartilage degradation in CIA mice knee joints were shown to have been suppressed by FAN. These findings suggest that fangchinoline has the potential to be a therapeutic source for the treatment of rheumatoid arthritis.

• Parasites, Hosts and Diseases
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• 제57권6호
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• pp.699-704
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• 2019

Anisakiasis (anisakidosis) refers to a foodborne zoonosis caused by ingesting raw or undercooked marine fish or cephalopods infected with anisakid larvae. The present study was performed to investigate the prevalence of anisakid larvae in anchovies (Engraulis japonica) purchased from 2 local markets in Gyeongsangnam-do, the Republic of Korea (=Korea), during 2018-2019. Anchovies were transported to our laboratory and examined by pepsin-HCl artificial digestion technique followed by microscopic observations and molecular analyses. The overall prevalence of anisakid larvae was 19.5% (39/200), from which a total of 51 larvae (av. 1.3 larvae/infected anchovy) were recovered. Sequencing of the larvae targeting the ITS region, including ITS1, 5.8S rRNA, and ITS2 genes confirmed the species of larvae as Anisakis pegreffii (54.9%; 28/51), Hysterothylacium sinense (23.5%; 12/51), and Hysterothylacium aduncum (21.5%; 11/51). The results suggested that anchovies could be a potential source of human anisakiasis in Korea.

• Plant Biotechnology Reports
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• 제2권1호
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• pp.21-31
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• 2008

In plants, the oxygen generated by photosynthesis can be excited to form reactive oxygen species (ROS) under excessive sunlight. Excess ROS including singlet oxygen ($^1O_2$) inhibit the growth, development and photosynthesis of plants. To isolate ROS-resistant crop plants, we used paraquat (PQ), a generator of $O_2{^-}$ as a source of screening and mutagen, and obtained two PQ-resistant lines in Pisum sativum, namely R3-1 and R3-2. Both lines showed greater resistance to PQ than their wild type (WT) siblings with respect to germination, root growth, and shoot growth. Biochemical analysis showed differences in these lines, in which ROS-scavenging enzymes undergo changes with a distinguishable increase in Mn-SOD. We further observed that the cytosolic catalases (CATs) in leaves in both lines were shifted in a native-PAGE analysis compared with that of the WT, indicating that the release of bound $^1O_2$ was enhanced. Phenotypic analysis revealed distinguishable differences in leaf development, and in flowering time and position. In addition, R3-1 and R3-2 showed shorter individual inter-node lengths, dwarf plant height, and stronger branching compared with the WT. These results suggested that PQ-induced ROS-resistant Pisum have the potential pleiotropic effects on flowering time and stem branching, and that ROS including $^1O_2$ plays not only important roles in plant growth and development as a signal transducer, but also appears as a strong inhibitor for crop yield.

• 비파괴검사학회지
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• 제22권1호
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• pp.32-37
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• 2002

산업현장에서 수소는 설비를 손상시킬 수 있는 주원인 중의 하나이며, 종종 설비를 파괴시키는 사고를 발생시킨다. 수소손상에 의한 결정립계의 공동 또는 미세균열은 강재의 파괴인성과 강도를 떨어뜨리는 원인이 되고, 따라서 과거 제한적인 방법으로 수소손상을 평가하기 위한 시도가 수행되었다. 본 연구에서는 초음파를 적용하여 수소손상을 검출하고 평가하기 위한 연구를 수행하였다. 미세조직 시험에 의해 확인된 시험편을 이용하여 초음파의 속도와 감쇠계수를 구한 결과, 수소손상에 의해서 초음파 속도는 감소하고 감쇠는 현저하게 증가하는 것으로 나타났다. 이러한 결과를 바탕으로 수소손상을 검출하기 위한 신뢰성 있는 평가법을 제시하였다.

• 공업화학
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• 제23권2호
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• pp.125-130
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• 2012

현재 화석연료사용의 급증으로 인한 지구온난화와 자원고갈의 문제가 전세계적으로 크게 대두되어지고 있다. 이를 해결하기 위해 세계적으로 재생 가능한 바이오매스의 개발에 관심을 기울이고 있다. 바이오가스는 다양한 바이오매스로부터 생산된 기체상태의 연료로 전력생산 및 기존 난방용 연료와 자동차연료로 대체할 수 있는 친환경적 석유대체연료이다. 우리나라의 유기성폐기물은 발생량의 상당부분을 차지하는 음식물쓰레기와 가축분뇨는 매년 증가 추세에 있으며, 유기성폐기물에서 발생하는 가스는 60% 이상의 고농도 메탄을 함유하고 있다. 이를 에너지원으로 이용할 경우 에너지의 효율적 이용에 큰 효과를 기대할 수 있다. 본 논문에서는 국내 신재생에너지로서 바이오가스의 현황을 살펴보고자 한다.

• 한국식품과학회지
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• 제49권4호
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• pp.377-382
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• 2017

본 연구에서는 한국 전통식품인 김치에서 분리된 LAB를 이용하여 콜레스테롤 감소능을 가지고 있는 LAB를 분리하고 그 특성을 규명하였다. 23 종의 분리 LAB 중에서 L. plantarum FMB 31 균주가 99.4%의 높은 콜레스테롤 감소 활성을 나타내었고, 프로바이오틱스로서 활용하기 위한 중요한 특성인 내담즙성, 내산성 그리고 내염성 등이 대조구로 사용된 L. rhamnosus KCTC 5033 균주보다 우수하거나 동등하였다. 또한 L. plantarum FMB 31 균주는 인체 안전성과 관련된 용혈독성 및 생체아민 생성능 실험에서도 안전성에 문제가 없는 것으로 분석되었다. 향후, 이 분리균은 콜레스테롤 저하 기능을 가지는 다양한 건강 기능 식품 개발에 이용될 수 있을 것으로 기대된다.

• 자원환경지질
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• 제37권3호
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• pp.311-324
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• 2004

전라남도 보성강에 축조된 주암댐의 집수유역 내 하상퇴적물을 대상으로 중금속의 오염현황과 거동 특성을 알아보았다. 주암댐의 집수유역에는 많은 폐금속 및 탄광이 위치하고 있어 주암댐 호저퇴적물에 잠재적인 오염원으로 작용할 수 있다. 집수유역 내 하상퇴적물의 중금속 함량(Cr, Cu, Ni, Pb, Zn)은 매우 낮기 때문에 주암댐의 호저퇴적물에 영향을 줄 것으로 판단되지 않는다. 하지만 폐금속광산지역 내 하상퇴적물의 Pb 함량은 하류로 갈수록 증가하는 경향을 보여, Pb의 오염 확산이 우려된다. 폐금속광산의 환경을 고려한 강산성 조건의 용출실험결과, 하상퇴적물의 용출비로부터 결정된 중금속의 상대적 이동도는 Pb＞Zn=Cu＞Ni＞Cr으로서, Pb이 주암댐 호저퇴적물에 영향을 미치는 원소가 될 수 있음을 지시해준다. 한편 댐으로 유입한 퇴적물이 호저에 놓이게 되면(즉, 환원환경), Pb의 이동도가 비교적 높게 나타나 물-퇴적물 경계에서 Pb의 수중으로 재용출될 수 있다.