• Title/Summary/Keyword: Potential pathogen

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Screening for In Vitro Antifungal Activity of Soil Bacteria Against Plant Pathogens

  • Chang, Sung-Hwan;Lee, Jung-Yeop;Kim, Ki-Deok;Hwang, Byung-Kook
    • Mycobiology
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    • v.28 no.4
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    • pp.190-192
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    • 2000
  • Antifungal bacteria for biological control of plant diseases or production of novel antibiotics to plant pathogens were isolated in 1997 from various soils of Ansung, Chunan, Koyang, and Paju in Korea. Sixty-four bacterial strains pre-screened from approximately 1,400 strains were tested on V-8 juice agar against eight plant pathogenic fungi using in vitro bioassay technique for inhibition of mycelial growth. Test pathogens were Alternaria mali, Colletotrichum gloeosporioides, C. orbiculare, Fusarium oxysporum f. sp. cucumerinum, F. oxysporum f. sp. lycopersici, Magnaporthe grisea, Phytophthora capsici, and Rhizoctonia solani. A wide range of antifungal activity of bacterial strains was found against the pathogenic fungi, and strain RC-B77 showed the best antifungal activity. Correlation analysis between inhibition of each fungus and mean inhibition of all eight fungi by 64 bacterial strains revealed that C. gloeosporioides would be best appropriate for detecting bacterial strains producing antibiotics with potential as biocontrol agents for plant pathogens.

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Stimulation of Platelet-Activating Factor (PAF) Synthesis in Human Intestinal Epithelial Cell Line by Aerolysin from Aeromonas encheleia

  • Nam In-Young;Cho Jae-Chang;Myung Hee-Joon;Joh Ki-Seong
    • Journal of Microbiology and Biotechnology
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    • v.16 no.8
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    • pp.1292-1300
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    • 2006
  • Aeromonas encheleia, a potential human intestinal pathogen, was shown to infect a human intestinal epithelial cell line (Caco-2) in a noninvasive manner. The transcriptional profile of the Caco-2 cells after infection with the bacteria revealed an upregulated expression of genes involved in chloride secretion, including that of phospholipase A2 (PLA2) and platelet-activating factor (PAF) acetylhydrolase (PAFAH2). This was also confirmed by a real-time RT-PCR analysis. As expected from PLA2 induction, PAF was produced when the Caco-2 cells were infected with the bacteria, and PAF was also produced when the cells were treated with a bacterial culture supernatant including bacterial extracellular proteins, yet lacking lipopolysaccharides. Bacterial aerolysin was shown to induce the production of PAF.

Needs of Biosecurity and Protocols for the Environmental Management of Carcasses Burial (가축매몰지 환경관리에 있어 차단방역의 필요 및 절차)

  • Cho, Ho-Seong;Kim, Geonha
    • Journal of Korean Society on Water Environment
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    • v.28 no.2
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    • pp.305-312
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    • 2012
  • Avian influenza (AI) and foot and mouth disease (FMD) are two main contagious pathogenic viruses causing massive mortality burial, as burial is a primary measure to quaranteen the causative viruse(s). Biosecurity is a set of preventive measures designed to reduce the risk of propagation of infectious diseases. Main objectives of this paper were to discuss the needs of biosecurity and develop protocol outlines for environmental management of burial sites. Pathological characteristics of contagious viruses should be considered during environmental management practices. Current practice prescribes to minimize the potential for on-farm pollution and the spread of infectious diseases, policy makers should understand robust knowledge regarding biosecurity to make informed decisions on future legislation.

Probiotics and Intestinal Health (유산균 Probiotics와 장내 건강)

  • Bang, Miseon;Lee, Sang Dae;Oh, Sejong
    • Journal of Dairy Science and Biotechnology
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    • v.30 no.2
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    • pp.139-143
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    • 2012
  • For human including newborn baby, the intestinal microbiota can play an important role in the development of the intestinal mucosa and in maintaining the balance of the immune cells. Important functions of the intestinal microbiota include the inhibition of the colonization of the intestine by potentially pathogenic microorganisms. Thus, the research of probiotics have been focused on the prevention and treatment of disorders associated with the gastrointestinal tract (GIT), including pathogen infection, traveler's diarrhea, antibiotic-associated diarrhea, and constipation. Probiotics have also been suggested as therapeutic agents against irritable bowel syndrome and inflammatory bowel diseases. An increasing amount of evidence from clinical studies suggests that they are effective in the prevention of atopic allergies and may have potential anti-carcinogenic effects. Until recent years many scientific research for this use has been based on empirical observations. Therefore, probiotics in the form of fermented milk products have been long part of attempts to maintain good health in world wide.

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Biosensors and their Applications in Food Safety: A Review

  • Yasmin, Jannat;Ahmed, Mohammed Raju;Cho, Byoung-Kwan
    • Journal of Biosystems Engineering
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    • v.41 no.3
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    • pp.240-254
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    • 2016
  • Background: Foodborne pathogens are a growing concern with respect to human illnesses and death. There is an increasing demand for improvements in global food safety. However, it is a challenge to detect and identify these harmful organisms in a rapid, responsive, suitable, and effective way. Results: Rapid developments in biosensor designs have contributed to the detection of foodborne pathogens and other microorganisms. Biosensors can automate this process and have the potential to enable fast analyses that are cost and time-effective. Various biosensor techniques are available that can identify foodborne pathogens and other health hazards. Conclusions: In this review, biosensor technology is briefly discussed, followed by a summary of foodborne pathogen detection using various transduction systems that exhibit specificity for particular foodborne pathogens. In addition, the recent application of biosensor technology to detect pesticides and heavy metals is briefly addressed.

Serratia plymuthica Strain A2l-4: A Potential Biocontrol Agent Against Phytophthora Blight of Pepper

  • Shen, Shun-Shan;Kim, Jin-Woo;Park, Chang-Seuk
    • The Plant Pathology Journal
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    • v.18 no.3
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    • pp.138-141
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    • 2002
  • A promising biocontrol agent, A2l-4, against Phytophthora blight of pepper was selected from 351 bacterial isolates collected from rhizosphere soils and roots of onion (Allium fistulosum L.). The isolate A21-4 was identified as Serratia plymuthica based on its 16S rRNA sequence and key characteristics as compared with that of an authentic culture of S. plymuthica (ATCC No. 6109D01). The isolate readily colonized on roots of various crops including pepper when inoculated on seed and not. Strain A2l-4 showed narrow spectrum of antibiotic activity, as revealed in its strong inhibitory activity to the genera Pythium and Phytophthora, but not to Fuasrium and Rhizoctonia. In pot experiments, none of the pepper seedlings treated with A2l-4 were infected by Phytophthora capsici, while 86% of the control plants were killed by the pathogen.

Antibacterial Activity of Acanthoic acid Isolated from Acanthopanax koreanum against Oral and Skin Microfloras

  • Kim, Jin-Kyung
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.20 no.6
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    • pp.1625-1628
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    • 2006
  • The (-)-pimara-9 (11), 15-dien-19-oic acid, acanthoic acid was extracted from the roots of Acanthopanax koreanum using bioassay-guided isolation of a MeOH extract. Acanthoic acid was assayed against Streptococcus mutans and Staphylococcus epidermidis causing dental caries and opportunistic pathogen. The minimum inhibitory concentration (MIC) of acanthoic acid against Streptococcus mutans and Staphylococcus epidermidis was 2 and 4 ${\mu}g/mL$, respectively, which was much lower than those of other natural antimicrobial agents such as 8 ${\mu}g/mL$ of tanshinone IIA. Acanthoic acid also significantly inhibited the growth of other cariogenic bacteria such as Streptococcus sobrinus and Streptococcus sanguis, and Streptococcus grodenii in the MIC range of 4${\sim}$32 ${\mu}g/mL$. Our findings suggest that acanthoic acid could be employed as a potential antibacterial agent for preventing dental caries and skin infections.

Phacoclastic Endophthalmitis Induced by Encephalitozoon cuniculi in a Lionhead Rabbit in Korea

  • Kang, Seonmi;Jeong, Seowoo;Seo, Kangmoon
    • Journal of Veterinary Clinics
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    • v.37 no.6
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    • pp.345-349
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    • 2020
  • An 8-month-old female rabbit was presented with a white intraocular mass in the right eye. Slit-lamp biomicroscopy showed a white mass behind the iris, accompanied by rubeosis iridis and aqueous flare. Ocular B-scan ultrasonography revealed hyperechoic material within the anterior chamber connected with cataractous lens in the right eye. Signs deteriorated despite treatment, and enucleation was performed. Histopathologically, phacoclastic endophthalmitis due to Encephalitozoon cuniculi infection was confirmed. This was the first report of a client-owned rabbit affected with E. cuniculi-associated phacoclastic uveitis. Serological detection of anti-E. cuniculi antibodies should be considered to prevent potential zoonotic risk.

Antibacterial compounds in green microalgae from extreme environments: a review

  • Little, Shannon M.;Senhorinho, Gerusa N.A.;Saleh, Mazen;Basiliko, Nathan;Scott, John A.
    • ALGAE
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    • v.36 no.1
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    • pp.61-72
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    • 2021
  • Increased proliferation of bacterial resistance to antibiotics is a critical issue that has increased the demand for novel antibacterial compounds. Antibacterial activities have been evaluated in extracts from photosynthetic green microalgae, with varying levels of subsequent potential for development based on the strain of algae, strain of bacterial pathogen, and solvent used to extract the metabolites. Green microalgae from extreme environmental conditions have had to adapt to conditions that exclude many other organisms. The production of antibacterial compounds aids directly or indirectly in the survival of green microalgae in these extreme environments, as well as potentially serve other roles. This review investigates antibacterial activities of green microalgae from both extreme in-situ environmental conditions and induced extreme laboratory conditions and highlights.

Bacterial Community Structure and the Dominant Species in Imported Pollens for Artificial Pollination

  • Kim, Su-Hyeon;Do, Heeil;Cho, Gyeongjun;Kim, Da-Ran;Kwak, Youn-Sig
    • The Plant Pathology Journal
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    • v.37 no.3
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    • pp.299-306
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    • 2021
  • Pollination is an essential process for plants to carry on their generation. Pollination is carried out in various ways depending on the type of plant species. Among them, pollination by insect pollinator accounts for the most common. However, these pollinators have be decreasing in population density due to environmental factors. Therefore, use of artificial pollination is increasing. However, there is a lack of information on microorganisms present in the artificial pollens. We showed the composition of bacteria structure present in the artificial pollens of apple, kiwifruit, peach and pear, and contamination of high-risk pathogens was investigated. Acidovorax spp., Pantoea spp., Erwinia spp., Pseudomonas spp., and Xanthomonas spp., which are classified as potential high-risk pathogens, have been identified in imported pollens. This study presented the pollen-associated bacterial community structure, and the results are expected to be foundation for strengthening biosecurity in orchard industry.