• Bulletin of the Korean Chemical Society
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• 제33권9호
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• pp.2883-2889
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• 2012

LL-37 is the only antimicrobial peptide (AMP) of the human cathelicidin family. In addition to potent antimicrobial activity, LL-37 is known to have the potential to inhibit lipolysaccharide (LPS)-induced endotoxic effects. To provide the stability to proteolytic digestion and increase prokaryotic selectivity and/or anti-endotoxic activity of two Lys/Trp-substituted 19-meric antimicrobial peptides (a4-W1 and a4-W2) designed from IG-19 (residues 13-31 of LL-37), we synthesized the diastereomeric peptides (a4-W1-D and a4-W2-D) with D-amino acid substitution at positions 3, 7, 10, 13 and 17 of a4-W1 and a4-W2, respectively and the enantiomeric peptides (a4-W1-E and a4-W2-E) composed D-amino acids. The diastereomeric peptides exhibited the best prokaryotic selectivity and effective protease stability, but no or less anti-endotoxic activity. In contrast, the enantiomeric peptides had not only prokaryotic selectivity and anti-endotoxic activity but also protease stability. Our results suggest that the hydrophobicity and ${\alpha}$-helicity of the peptide is important for anti-endotoxic activity. In particular, the enantiomeric peptides showed potent anti-endotoxic and LPS-neutralizing activities comparable to that of LL-37. Taken together, both a4-W1-E and a4-W2-E holds promise as a template for the development of peptide antibiotics for the treatment of endotoxic shock and sepsis.

• 한국정밀공학회지
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• 제22권3호
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• pp.115-122
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• 2005

As the digital TV markets rapidly growing, many manufacturers introduce large size flat screen TV units. There are two different display types available to large size models which are plasma and TFT-LCD. Since both are constructed with thin large panels that are mostly fragile to even moderate mechanical shock inputs. Some large size panels are severely resonated by the acoustic sound generated TV which deteriorates video quality. Recognizing the potential problems of large displays, accurate measurement of the panels is to be an essential task for the reliable design. Measurement of mechanical properties of a thin large crystallized panel such as TFT-LCD display with traditional material testing equipments is challenging. Since TFT-LCDs are constructed with combination of brittle glass panels, polymer sheets, and liquid crystal, their properties are not only anisotropic but also usually non-linear. Accurate measurement of the properties often requires very expensive facilities. Especially when the size of the test sample is as large as 40-inch or wider, direct measurement cost is prohibitive. Even worse, machining of the large TFT-LCD to make a smaller size specimen that could be fit into a material tester is not possible because of liquid crystal leakage. A new method fer the measurement of elastic modulus of large TFT-LCD panel is presented in this article. The suggested method provides a simple, economic, and user-friendly way fer measuring the elastic modulus of large panels with considerable level of accuracy.

• 대한교통학회지
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• 제27권3호
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• pp.71-77
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• 2009

유비쿼터스 교통환경이 구현되면 기존 ITS 환경에서는 불가능하였던, 개별차량 위치, 속도 등 미세한 데이터 수집이 가능해 지며, V2V(Vehicle-to-Vehicle), V2I(Vehicle-to-Infra) 양방통신이 가능해 짐에 따라 개별차량 혹은 차량군 단위의 미세 제어가 가능해 진다. 이러한 유비쿼터스 교통 환경의 장점에 입각하여 교통류 안정성을 지표로 하는 연속류 정체예방관리 방안이 선행연구에서 제시된 바 있다. 본 연구에서는 유비쿼터스 교통환경에서 정체예방관리를 실현함에 있어 제기된 쟁점사항들에 대하여, VISSIM 시뮬레이션 실험결과에 입각하여 해답을 제시하였다. 교통류 안정성을 명시적으로 교통류 관리에 적용할 수 있도록, 유비쿼터스 교통환경에서 수집 가능한 차량군 길이와 간격으로 도출하였다. 또한 교통상황에 따라 차별화된 운영관리가 필요하다는 타당성을 도출하고, 교통상황 구분 및 상황별 운영관리 방안을 제시하였다. 마지막으로 이러한 관리를 수행하였을 때 어떠한 잠재적 이익이 있는가를 보여주었다. 본 연구 결과는, 제반 제약조건으로 말미암아 제한적인 시뮬레이션 실험을 통해 도출되었다. 그러나 유비쿼터스 교통환경에서 새로운 개념의 교통관리를 시행하는 데 있어 그 타당성은 보여줄 수 있었다고 판단된다. 향후 보다 포괄적인 현장실험을 통해 제반 결과들이 확정되어야 할 것이다.

• Tribology and Lubricants
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• 제36권4호
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• pp.244-252
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• 2020

Materials manufactured by powder metallurgy (PM) are widely used in various applications such as water pump, shock absorber, and airplane components due to the reduction in the cost and weight. In this study, tribological properties of carbon steel subjected by surface treatment were investigated. The main purpose is to increase the strength and improve the tribological properties by reducing pores that formed by PM. Moreover, the surface treatment was carried out at room and high temperatures (RT and HT). The surface roughness of the untreated (NON) and treated (AFTER) samples was measured. It was found that the surface roughness was reduced after both the RT AFTER and HT AFTER compared to RT NON sample. The tribological properties of the samples were performed against bearing steel ball under dry conditions. The friction coefficient of the RT NON samples was reduced by 22% and 56% RT AFTER and HT AFTER, respectively. The wear volume of the RT NON sample was also reduced by 43% and 87% RT AFTER and HT AFTER, respectively. Tribocorrosion tests were also performed and it was found that the surface of the RT AFTER, HT AFTER samples was less corroded compared to RT NON sample. The HT AFTER sample demonstrated a relatively higher corrosion potential in comparison with the RT AFTER samples. Hence, it was confirmed that after surface modification the surface roughness and hardness of the samples were significantly improved resulting in improvement in tribological and tribocorrosion behaviors of PM carbon steel.

• 대한본초학회지
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• 제25권3호
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• pp.35-41
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• 2010

Objectives : Allergy is an immune dysfunction caused by degranulation from mast cells in the early phase of allergic disease including allergic rhinitis (AR). The purpose of this study was to investigate the effects of Osterici Radix, roots of Ostericum koreanum Maximowicz in human mast cells and experimental allergic animal models. Methods : The anti-allergic effect of Osterici Radix water extract (NK-W) was investigated in human mast cell line, HMC-1 cells, and compound 48/80-induced systemic anaphylactic response in rats and ovalbumin (OVA)-induced AR in mice. Animals were orally administrated with NK-W (10 and 50 mg/kg) or anti-histamine drug, dosodium cromoglycate (50 mg/kg), and then intraperitoneally injected with compound 48/80 (8 mg/kg) or sensitized with 0.1% OVA into nasal. Animals were observed plasma histamine and histological changes of nasal mucosa. Also, mast cell degranulation and histamine production were determined in compound 48/80-stimulated HMC-1 cells. Results : NK-W inhibited compound 48/80-induced degranulation of mast cells and histamine releasing in HMC-1 cells. NK-W decreased mortality and serum histamine releasing in compound 48/80-induced anaphylatic rats in a dose-dependently manner. NK-W also inhibited serum histamine levels in OVA-induced AR mice and improved abnormal histological changes such as expansion of grandular cells and hypertrophy of epithelium in the nasal mucosa. These results indicate that Osterici Radix water extract suppress allergic response through downregulation of mast cell activation. Conclusions : This study suggests that a therapeutic potential of Osterici Radix as a source of anti-allergic agents for use in a number of allergic diseases.

• Nuclear Engineering and Technology
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• 제26권1호
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• pp.63-77
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• 1994

이 연구는 가압경수로의 원자로 다운커머내에서 과도냉각시 직접용기주입에 따른 유체혼합현상을 가압열충격의 견지에서 시험모델을 사용하여 조사한 것이다. 시험모델은 ABB-CE System80＋ 원자로 구조에 근거하여 설계되었다. 이 원자로에 대한 가능성 있는 가압열충격 사고로서 콜드레그 소형파단 냉각재 상실사고와 주중기관 판단 사고가 선정되었다. 시험은 두 부분으로 구성되는데 첫째 부분은 원자로 다운커머에서 직접용기 주입수와 기존냉각재간의 유체혼합을 가시화법에 의하여 시험한 것이고, 둘째 부분은 별도의 시험모델에서 직접용기주입에 따른 열적혼합을 시험한 것이다. 가시화 시험에서는 과도적 냉각기간중 직접용기 주입수와 1차 냉각재간의 물리적 상호작용이 밝혀졌다. 열적혼합시험에서는 소형파단 냉각재 상실사고시 직접용기주입에 의한 심한 냉각현상이 다운커머내서 관찰되었다. 측정된 온도곡선은 소형파단 냉각재 상실사고에 대하여 REMIX 로드, 증기관 파단사고에 대하여는 COM-MIX-1B 코드에 의한 계산과 비교되었다.

• 비파괴검사학회지
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• 제29권4호
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• pp.299-307
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• 2009

광섬유 센서 기술은 기존의 상용 센서의 어려움을 극복할 수 있어 전자기학 잡음과 전기 쇼크의 영향이 강한 폭발환경에서도 충분히 사용이 가능하다. 최근 이러한 장점들로 인해 여러 종류의 광섬유 센서들이 활발히 연구 개발되고 있다. 또한 비파괴검사/평가 분야로써 구조 건전성 감시를 위한 광섬유 센서의 다양한 적용 연구 분야가 존재한다. 그러나 로드셀과 같은 종류의 센서들은 상대적으로 상용화가 미흡한 실정이다. 본 논문에서는 광섬유 브래그 격자 센서를 사용한 광섬유 로드셀을 보여준다. 본 로드셀의 형상은 링크타입이고, 세 개의 광섬유 브래그 격자 센서를 사용하여 세 지점의 변형률을 각기 측정한다 특히 이들 변형률은 온도와 같은 동상 잡음을 제거하기 위해 차동법을 사용하여 신호처리 된다. 더 나아가 본 로드셀의 감도, 선형성 그리고 해상도를 인장실험을 통해 성공적으로 검증하였다.

• Journal of Chest Surgery
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• 제50권3호
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• pp.153-162
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• 2017

Background: The mesenchymal-epithelial transition factor (MET) receptor can be overexpressed in solid tumors, including small cell lung cancer (SCLC). However, the molecular mechanism regulating MET stability and turnover in SCLC remains undefined. One potential mechanism of MET regulation involves the C-terminus of Hsp70-interacting protein (CHIP), which targets heat shock protein 90-interacting proteins for ubiquitination and proteasomal degradation. In the present study, we investigated the functional effects of CHIP expression on MET regulation and the control of SCLC cell apoptosis and invasion. Methods: To evaluate the expression of CHIP and c-Met, which is a protein that in humans is encoded by the MET gene (the MET proto-oncogene), we examined the expression pattern of c-Met and CHIP in SCLC cell lines by western blotting. To investigate whether CHIP overexpression reduced cell proliferation and invasive activity in SCLC cell lines, we transfected cells with CHIP and performed a cell viability assay and cellular apoptosis assays. Results: We found an inverse relationship between the expression of CHIP and MET in SCLC cell lines (n=5). CHIP destabilized the endogenous MET receptor in SCLC cell lines, indicating an essential role for CHIP in the regulation of MET degradation. In addition, CHIP inhibited MET-dependent pathways, and invasion, cell growth, and apoptosis were reduced by CHIP overexpression in SCLC cell lines. Conclusion: C HIP is capable of regulating SCLC cell apoptosis and invasion by inhibiting MET-mediated cytoskeletal and cell survival pathways in NCI-H69 cells. CHIP suppresses MET-dependent signaling, and regulates MET-mediated SCLC motility.

• Environmental Engineering Research
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• 제10권4호
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• pp.181-190
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• 2005

Severe loss or hydrogen occurred in most anaerobic hydrogen fermentation reactors. Several selected methods were applied to suppress the consumption of hydrogen and increase the potential of production. As the first trial, pH shock was applied. The pH of reactor was dropped nearly to 3.0 by stopping alkalinity supply and on]y feeding glucose (5 g/L-d). As the pH was increase to $4.8{\pm}0.2,$ the degradation pathway was derived to solventogenesis resulting in disappearance of hydrogen in the headspace. In the aspect of bacterial community, methanogens weren't detected after 22 and 35 day, respectively. Even though, however, there was no methanogenic bacterium detected with fluorescence in-situ hybridization (FISH) method, hydrogen loss still occurred in the reactor showing a continuous increase of acetate when the pH was increased to $5.5{\pm}0.2$. This result was suggesting the possibility of the survival of spore fanning acetogenic bacteria enduring the severely acidic pH. As an alternative and additive method, nitrate was added in a batch experiment. It resulted in the increase of maximum hydrogen fraction from 29 (blank) to 61 % $(500\;mg\;NO_3/L)$. However, unfortunately, the loss of hydrogen occurred right after the depletion of nitrate by denitrification. In order to prevent the loss entangled with acetate formation, $CO_2$ scavenging in the headspace was applied to the hydrogen fermentation with heat-treated sludge since it was the primer of acetogenesis. As the $CO_2$ scavenging was applied, the maximum fraction of hydrogen was enhanced from 68 % to 87 %. And the loss of hydrogen could be protected effectively.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2001년도 춘계학술발표대회
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• pp.40-40
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• 2001

Heat stress to bovine oocytes and embryos has suggested a potential role of retardation of their development. Limited study has reported on the effect of heat shock on sperm before using it for IVF. Caudal epididymal sperm cultured in 42$^{\circ}C$ incubator for 0.5, 1 and 2 h compared on sperm viability and oocyte development after its use for IVF to those of control. Oocytes were matured for 22 h and then inseminated with treated or control sperm for 16 h. Embryos were cultured in CRlaa medium, transferred to TCM199＋10% FBS on day 4, and maintained on day 9. A higher proportion (84.1%, 0.5 h; 72%, 1 h: 65%, 2 h) in treated sperm was observed dead and abnormal pattern as 100% of consider as control. In control the rates of cleavage and development into blastocyst were 76% and 22%, respectively, and did not differ the rates between 1 h and 2 h of culture. Significant differences were appeared in the rates between treated for an hour and control (32% and 5% vs. 54% and 10%, respectively). Moreover increased time of culture is more retardation to be cleaved the oocytes. However, the rates of blastocyst from cleaved embryos in treated group similar to control (25% vs. 29%, respectively). The reason for this remains unclear, but male sperm, from preliminary experiment(data un-shown) for sexing of resulting embryos, would be more fragility on heat stress.