• Title/Summary/Keyword: Potato cultivation

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Gamma-ray Irradiation on Radio Sensitivity in Yacon (Samallanthus sonchifolius (Poepp. & Endl.) H. Robinson) Breeding (돌연변이 육종을 위한 야콘의 최적 감마선 조사량)

  • Su Jeong Kim;Hwang Bae Sohn;Yul Ho Kim;Jung Hwan Nam;Jong Nam Lee;Dong Chil Chang;Jong Taek Suh
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2021.04a
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    • pp.27-27
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    • 2021
  • Yacon [Samallanthus sonchifolius (Poepp. & Endl.) H. Robinson], a member of Compositae plants, has sweet taste and crisp texture. Unlike other Andean root crops such as potato and sweet potato, the cultivation area of yacon has increased recently, since it is known to have large content of fructooligosaccharides (FOS). Since there are no yacon varieties bred in Korea, we have been trying to create new genetic resources using gamma-ray. The optimal gamma-ray dosage for mutation breeding in yacon was investigated. Crown bud and green bud of yacon were exposed to doses of gamma rays from 20 Gy to 80 Gy, and subsequently planted in a greenhouse. After 50 days of sowing, the survival rates and growth decreased rapidly at doses above 40 Gy, while all of crown bud individuals died above 60 Gy. The median lethal dose (LD50) of crown bud and green bud was 22.4 and 36.6 Gy, and the median reduction doses (RD50) for plant height, fresh weights, and tuberous root weight were 20-40 Gy, respectively. A dose of 20-40 Gy was found to be optimal for mutation breeding in yacon. Considering the growth factors, the optimum doses were determined to be within the range of 20-40 Gy for the selection of useful mutant lines. M2-M3 mutant lines were obtained from 20-60 Gy gamma-ray-irradiated M1 plants through clonal propagation. These mutant lines will be used for the development of a new variety of yacon plant with high FOS and no crack tuberous root.

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Physiology characteristics of genus Hypocrea isolated at oyster mushrooms of the shelf cultivation (균상재배 느타리버섯에서 분리한 Hypocrea속 균의 생리적 특성)

  • Lee, Chan-Jung;Moon, Ji-Won;Cheong, Jong-Chun;Kong, Won-Sik;Jhune, Chang-Sung
    • Journal of Mushroom
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    • v.13 no.1
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    • pp.68-73
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    • 2015
  • Hypocrea disease is the most severe disease of oyster mushroom cultivation in Korea. Physiological and ecological studies were performed on the pathogens (Hypocrea spp.) to obtain basic information for developing the integrated disease management system. Fourteen isolates of Hypocrea were collected from oyster mushroom house in five areas. Pathogenic fungi causing disease of oyster mushroom were identified as Hypocrea sp. based on morphological characteristics and pathogenicity. Two isolates (H-1, H-12) showed the fastest growth at $15^{\circ}C$ but four isolates (H-8, H-9, H-13, H-14) showed slower growth than those of other isolates at $20^{\circ}C$ and $25^{\circ}C$. Stroma with ascocarps and ascospore were produced on PDA under fluorescent light. The five isolates produced stroma with ascocarps and ascospores. Formation of fruiting body of strains H-14 of Hypocrea were the best out of all the strains on the potato dextrose agar (PDA). Also, fruiting bodies and ascospores were completely produced under fluorescent light. The growth of the isolates was correlated with total carbon content. The stroma of the isolates was formed mainly in histidine and asparagine treatment and especially in histidine-70 and asparagine-100 treatment. In the test of pathogenicity, after and before spawning showed very fast incidence of disease.

Isolation and identification of a tricin 4"-O-(threo-β-guaiacylglyceryl) ether producing microorganism from germinated rice (발아 벼로부터 tricin 4"-O-(threo-β-guaiacylglyceryl) ether 생성균주의 분리 및 동정)

  • Yoon, Nara;Jang, Gwi Yeong;Lee, Yoon Jeong;Li, Meishan;Kim, Min Young;Kim, Hyun Young;Lee, Junsoo;Jeong, Heon Sang
    • Korean Journal of Food Science and Technology
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    • v.48 no.4
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    • pp.361-365
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    • 2016
  • This study was conducted to isolate and identify a microorganism that increases tricin-O-(threo-${\beta}$-guaiacylglyceryl) ether (TTGE) content in the hulls of rice (Oryza sativa L.). Bacteria from germinated rice were isolated by enrichment cultivation using yeast mold, luria bertani, potato dextrose and mannitol egg york polymyxin broths. The highest increase in TTGE content ($339.30{\mu}g/g$) was achieved by a microorganism isolated by PDA enrichment cultivation. On the basis of 16S RNA sequence homology and phylogenetic analysis, the isolated bacterium was identified to have 100% similarity with Burkholderia vietnamiensis. The isolated bacteria were short rods, negative for the Gram stain, and positive for the catalase test. The highest TTGE level was $435.86{\mu}g/g$ in 72-h fermented samples, representing a 2.5x increase compared with the control ($175.65{\mu}g/g$). In conclusion, the bacterium isolated from germinated rice extract was Burkholderia vietnamiensis, and the optimum fermentation period to maximize TTGE levels was 72 h. These findings might help in developing functional materials using rice hulls, a waste product of rice milling.

Isolation and Utilization of Antagonistic Pseudomonas fluorescens from Soils for the Protection of Soybean Sprouts Rot (콩나물 부패병 방제를 위해 토양으로부터 분리한 길항균 Pseudomonas fluorescens의 이용)

  • Kim, Jin-Ho;Joo, Gil-Jae;Choi, Yong-Hwa
    • Korean Journal of Environmental Agriculture
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    • v.20 no.1
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    • pp.50-56
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    • 2001
  • Thirty-three bacterial and fungal strains were isolated from the rotten soybeans and soybean sprouts to isolate pathogenic microorganisms which cause soybean sprouts rot during soybean sprouts cultivation. In pathogenicity tests of the isolates on soybean sprouts, two isolates(K-17 and K-28) caused soybean sprouts rot and were identified as Erwinia carotovora and Fusarium sp., respectively. To isolate antagonists aganist K-17 and K-28 pathogens, bacteria were isolated from various soybean-cultivated soils and screened by the inhibition zone method. A bacterial isolate(J-232) which inhibited growth of both pathogens was identified as Pseudomonas fluorescens and further examined. The culture filtrate of P. fluorescens J-232 (dilution rate of 500 times) inhibited the growth of Erwinia carotovora K-17 and Fusarium sp. K-28 both on potato dextrose agar medium and on soybean sprouts cultivated in vessel. The development of soybean sprouts rots was observed during cultivation by inoculation of soybean seeds with culture filtrate of both pathogens. The combined inoculation of soybean seeds with culture filtrate of antagonistic bacterium and that of pathogens prevented soybean sprouts rot, and the growth of soybean sprouts was similar to that of control. The soybean sprouts inoculated with antagonists culture filtrate alone did not develop soybean sprouts rot, and the growth of the seedlings was shown to be slightly promoted as compared with that of control.

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Case studies for estimation nitrogen and phosphorous balance with different cropping systems in upland (현장사례를 통한 밭 작부의 질소와 인산양분 수지 평가)

  • Yun, Hong-Bae;Lee, Sang-Min;Kim, Myung-Sook;Lee, Ye-Jin;Lee, Yeoun;Lee, Jong-Sik
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.5
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    • pp.762-766
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    • 2011
  • Cropping system is very important for environment conservation and improvement of nutrient recovery rates in agricultural land. This case study was conducted to identify the nutrient balance of major upland cropping types with different districts. Typical cropping systems at MuAn and HaeNam located in the south coast district were cultivated with soybean-chinese cabbage or garlic and garlic-soybean-chinese cabbage-hot pepper. Alpine district, PyeongChang was cultivated with double cropping of chinese cabbage and potato-radish or chinese cabbage for one year. Typical cropping type of AnDong, YeongYang where are well known for hot pepper cultivation was hot pepper's mono cropping and hot pepper-soybean or hot pepper-sesame for one year. But SeoSan and HongSeong, where are located in mid-west coast had variety cropping system compareed to other districts. Double cropping of chinese cabbage, a heavy fertilizing crop, caused higher nutrient balance in the field when it was cultivated consistently. Experiment showed that heavy fertilizing crops, such as chinese cabbage, garlic and onion, need to be rotated with soybean and sesame cultivation which need smaller fertilizer level. Alpine arable land has shown higher nutrient balance than other areas, and the introduction of rotation crops are needed to reduce nutrient balance and environmental protection.

Response of Potassium on Main Upland Crops (주요(主要) 전작물(田作物)에 대(對)한 가리성분(加里成分)의 비교(肥效))

  • Ryn, In Soo
    • Korean Journal of Soil Science and Fertilizer
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    • v.10 no.3
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    • pp.171-188
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    • 1977
  • The response and effect on main upland crops to potassium were discussed and summarized as follows. 1. Adequate average amounts of potash per 10a were 32kg for forage crop; 22.5kg for vegetable crops; 17.3kg for fruit trees; 13.3kg for potatoes; and 6.5kg for cereal crops. Demand of potassium fertilizer in the future will be increased by expanding the acreage of forage crops, vegetable crops and fruit trees. 2. On the average, optimum potash rates on barley, wheat, soybean, corn, white potato and sweet potato were 6.5, 6.9, 4.5, 8.1, 8.9, and 17.7kg per 10a respectively. Yield increaments per 1kg of potash per 10a were 4-5kgs on the average for cereal crops, 68kg for white potato, and 24kg for sweet potato. 3. According to the soil testing data, the exchangeable potassium in the coastal area was higher than that in the inland area and medium in the mountainous area. The exchangeable potassium per province in decreasing order is Jeju>Jeonnam>Kangweon>Kyongnam. Barley : 4. The response of barley to an adequate rate of potassium seemed to be affected more by differences in climatic conditions than to the nature of the soil. 5. The response and the adequate rate of potassium in the southern area, where the temperature is higher, were low because of more release of potassium from the soil. However, the adequate rate of phosphorus was increased due to the fixation of applied phosphorus into the soil in high temperature regions. The more nitrogen application would be required in the southern area due to its high precipitation. 6. The average response of barley to potassium was lower in the southern provinces than northern provinces. Kyongsangpukdo, a southern province, showed a relatively higher response because of the low exchangeable potassium content in the soil and the low-temperature environment in most of cultivation area. 7. Large annual variations in the response to and adequate rates of potassium on barley were noticed. In a cold year, the response of barley to potassium was 2 to 3 times higher than in a normal year. And in the year affected by moisture and drought damage, the responses to potassium was low but adequate rates was higher than cold year. 8. The content of exchangeable potassium in the soil parent materials, in increasing order was Crystalline Schist, Granite, Sedimentary and Basalt. The response of barley to potash occurred in the opposite order with the smallest response being in Crystalline Schist soil. There was a negative correlation between the response and exchangeable potassium contents but there was nearly no difference in the adequate rates of potassium. 9. Exchangeable potassium according to the mode of soil deposition was Alluvium>Residium>Old alluvium>Valley alluvium. The highest response to potash was obtained in Valley alluvium while the other s showed only small differences in responses. 10. Response and adequate rates of potassium seemed to be affected greatly by differences in soil texture. The response to potassium was higher in Sandy loam and Loam soils but the optimum rate of potassium was higher in Clay and Clay loam. Especially when excess amount of potassium was applied in Sandy loam and Loam soils the yield was decreased. 11. The application of potassium retarded the heading date by 1.7 days and increased the length of culm. the number of spikelet per plant, the 1,000 grain weight and the ratio of grain weight to straw. Soybean : 12. Average response of soybean to potassium was the lowest among other cereal crops but 28kg of grain yield was incrased by applying potash at 8kg/10a in newly reclaimed soils. 13. The response in the parent materials soil was in the order of Basalt (Jeju)>Sedimentay>Granite>Lime stone but this response has very wide variations year to year. Corn : 14. The response of corn to potassium decreased in soils where the exchangeable potassium content was high. However, the optimum rate of applied potassium was increased as the soil potassium content was increased because corn production is proportional to the content of soil potassium. 15. An interaction between the response to potassium and the level of phosphorus was noted. A higher response to potassium and higher rates of applied potassium was observed in soils contained optimum level of phosphorus. Potatoes : 16. White potato had a higher requirement for nitrogen than for potassium, which may imply that potato seems to have a higher capability of soil potassium uptake. 17. The yield of white potato was higher in Sandy loam than in Clay loam soil. Potato yields were also higher in soils where the exchangeable potassium content was high even in the same soil texture. However, the response to applied potassium was higher in Clay loam soils than in Sandy loam soils and in paddy soil than in upland soil. 18. The requirement for nitrogen and phosphorus by sweet potato was relatively low. The sweet potato yield is relatively high even under unfavorable soil conditions. A characteristics of sweet potatoes is to require higher level of potassium and to show significant responses to potassium. 19. The response of sweet potato to potassium varied according to soil texture. Higher yields were obtained in Sandy soil, which has a low exchangeable potassium content, by applying sufficient potassium. 20. When the optimum rate of potassium was applied, the yields of sweet potato in newly reclaimed soil were comparable to that in older upland soils.

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Characteristics of Mycelial Growth and Enzyme Activities of Mattirolomyces terfezioides Collected from Robinia pseudoacacia Forest in Korea (국내 아까시나무 숲에서 수집한 감자덩이버섯(Mattirolomyces terfezioides)의 균사 생장 특성과 효소 활성)

  • Jeon, Sung-Min;Wang, Eun-Jin;Ka, Kang-Hyeon
    • The Korean Journal of Mycology
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    • v.43 no.3
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    • pp.165-173
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    • 2015
  • Mattirolomyces terfezioides is a type of sweet truffles that predominantly form ectomycorrhizae with Robinia pseudoacacia. It is also worthy of artificial cultivation. This is the first report on characteristics of mycelial growth and enzyme activities of M. terfezioides collected from R. pseudoacacia forest in Korea. M. terfezioides showed the highest mycelial growth when cultured on potato dextrose agar (PDA) at $30^{\circ}C$ or in modified Melin-Norkran's liquid medium (pH 8.0). The biomass of M. terfezioides was higher in liquid medium containing nitrate-nitrogen than ammonium-nitrogen by 1.8 fold. The mycelia of M. terfezioides showed both carboxymethylcellulase and laccase activities on solid media for enzyme screening.

Rice cultivars adaptable for rice based cropping systems in a paddy field in the Yeongnam plain area of Korea

  • Kim, Sang-Yeol;Seo, Jong-Ho;Bae, Hyun-Kyung;Hwang, Chung-Dong;Ko, Jong-Min
    • Korean Journal of Agricultural Science
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    • v.45 no.3
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    • pp.355-363
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    • 2018
  • This study was conducted to investigate suitable rice cultivars for various rice based cropping systems in a paddy field in the Yeongnam plain area. Thirteen rice cultivars (5 early, 3 medium and 5 mid-late maturing cultivars) and three transplanting dates (June 5, June 25 and July 5) were evaluated in this study. The mid-late and early maturing cultivars for the July 5 and June 25 transplanting headed before August 30 which is the safe heading date in the Yeongnam plain area, ranging from Aug. 26 - 28 and Aug. 18 - 23, respectively. The safe harvest time of rice for double and triple cropping systems should be before the middle of October for the cultivation of the succeeding winter crops. The rice yield was the highest for the June 5 transplanting regardless of the rice cultivars, and it gradually decreased as the transplanting date was delayed from June 5 to July 5 due to a decrease in the spikelet numbers per panicle number and in the ripened grain rate. In contrast, the other yield parameters that include the panicle number per $m^2$, the 1,000-brown rice weight, and the ripened grain ratio were not significantly affected. The result indicates that based on the milled rice, heading time and harvest time, a medium maturing cultivar (Haiami and Samdeog) would be applicable to a winter barley/wheat, garlic/onion-summer rice double cropping, while a mid-late cultivar (Saeilmi, Saenuri and Hyunpoom) would be suitable for a spring potato/waxy corn-summer rice double cropping in the Yeongnam plain area. On the other hand, an early maturing cultivar (Unkwang, Jokwang and Haedamssal) would be preferable for a triple cropping because of the short growth period of rice.

Screening of Selected Korean Sweetpotato (Ipomoea batatas) Varieties for Fusarium Storage Root Rot (Fusarium solani) Resistance

  • Lee, Seung-yong;Paul, Narayan Chandra;Park, Won;Yu, Gyeong-Dan;Park, Jin-Cheon;Chung, Mi-Nam;Nam, Sang-Sik;Han, Seon-Kyeong;Lee, Hyeong-Un;Goh, San;Lee, Im Been;Yang, Jung-Wook
    • The Korean Journal of Mycology
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    • v.47 no.4
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    • pp.407-416
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    • 2019
  • A common post-harvest disease of sweetpotato tuber is root rot caused by Fusarium solani in Korea as well as the other countries. Storage root rot disease was monitored earlier on sweetpotato (Ipomoea batatas) in storehouses of different locations in Korea. In the present study, an isolate SPL16124 was choosen and collected from Sweetpotato Research Lab., Bioenergy Crop Research Institute, NICS, Muan, Korea, and confirmed the identification as Fusarium solani by conidial and molecular phylogenetic analysis (internal transcribed spacer ITS and translation elongation factor EF 1-α gene sequences). The isolate was cultured on potato dextrose agar, and conidiation was induced. The fungus was screened for Fusarium root rot on tuber of 14 different varieties. Among the tested variety, Yenjami, Singeonmi, Daeyumi, and Sinjami showed resistant to root rot disease. Additionally, the pathogen was tested for pathogenicity on stalks of these varieties. No symptom was observed on the stalk, and it was confirmed that the disease is tissue specific.

Risk Assessment of Genetically Modified Organisms (유전자변형 생물체의 위해성평가)

  • 김형진;김환묵
    • Toxicological Research
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    • v.19 no.1
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    • pp.1-12
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    • 2003
  • New breeding method by genetic engineering is expected as a key technology to solve food shortage due to the growing world population in the year 2000s. Many genetically modified organisms (GMOs) were already developed and the commercial cultivation had started. The first GMO, Flavr Savr tomato, which rotted at a much slower pace than ordinary ones, was developed in US in 1994. Since then, over than 70 different agricultural products including corn, cotton, soybean, papaya, potato, and squash made with genetically modified plants are reportedly on sale worldwide. Supporters favor the GMOs because they have greater yields, longer shelf lives and stronger resistance to disease and insects. On the other hand, opponents say that the supporters ignore a potential danger that they may damage the environment as well as human beings. To assure the safe development and use of GMOs as food and other biotech products, the possible risks on biological environment and human health should be throughly examined and regulated by developer and government. Because the biosafety problem is a global, environmental, and trade issue, a new international treaty is under development. The Cartagena Protocol on Biosafety was adopted at the 1 st Extraordinary Conference of Parties of the Convention on Biological Diversity which was held at Mont-real, Canada, Jan. 29th, 2000. The adoption of the Protocol is seen as a breakthrough in that it is based on the" Precautionary Principle" despite scientific uncertainties surrounding potential risks that GMOs may inflict on human health and the environment and that it has laid the ground for introduction of specific steps to handle international trading of GMOs. In this paper, the authors would like to introduce the current status and perspective of environmental and human risk assessment of GMOs.t of GMOs.