• Title/Summary/Keyword: Potato Starch

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Improved Production, and Purification of Aclacinomycin A from Streptomyces lavendofoliae DKRS

  • Kim, Wan-Seop;Youn, Deok-Joong;Cho, Won-Tae;Kim, Myung-Kuk;Kim, Hak-Ryul;Rhee, Sang-Ki;Choi, Eui-Sung
    • Journal of Microbiology and Biotechnology
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    • v.5 no.5
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    • pp.297-301
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    • 1995
  • An anthracycline antibiotic, aclacinomycin A (aclarubicin), was produced from a mutant strain of Streptomyces lavendofoliae. The mutant strain which showed a 4-fold higher productivity of aclacinomycin A compared with the parent strain was also found to produce a significantly higher amount of aclacinomycin A than the reported production strain, Streptomyces galilaeus. The aclacinomycin A was produced up to 125 mg/l using potato starch and soybean meal as carbon and nitrogen sources, respectively, on a 3 liter scale fermentation in a 5 liter jar fermentor. The mutant strain also produced significant amount of aclacinomycins Band Y. Aclacinomycin A was isolated from the culture broth by solvent extractions and further purified by silica gel column chromatography. The yield of aclacinomycin A with over 99$%$ purity was found to be over 60$%$ starting from 3 liters of culture broth.

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Effects of Drying Condition and Binding Agent on the Quality Characteristics of Ground Dried-Pork Meat Products

  • Choi, Yun-Sang;Ku, Su-Kyung;Park, Jong-Dae;Kim, Hee-Ju;Jang, Aera;Kim, Young-Boong
    • Food Science of Animal Resources
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    • v.35 no.5
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    • pp.597-603
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    • 2015
  • The purpose of this study was to investigate the influence of processing conditions (temperature and time) and binding agent types (glutinous rice flour, potato starch, bean flour, and acorn flour) on the physicochemical and sensory characteristics of ground dried-pork meat product. For this purpose, ground dried-pork meat product was produced by adding several binding agents at different drying temperatures and times. The drying time affected moisture content and water activity in all drying temperature. However, under the similar drying conditions, the extent of drying varied depending on the type of binding agents. The results of sensory evaluation for texture degree and overall acceptability indicated the following: overall, higher drying temperatures and longer drying time heightened the degree of texture, and the overall acceptability varied depending on binding agent type. Physicochemical and sensory characteristics were analyzed to determine any possible correlation. The results revealed a high correlation between moisture content, water activity, shear forces, and sensory evaluation (p<0.01). However, there was no correlation with respect to overall acceptability.

Indirect Measurement of the Amounts of Casein Phosphopeptides Added to Dangmyon (Starch Vermicelli or Glass Noodle) as a Calcium-Absorption Enhancer

  • Choi, Jong-Woo;Kim, Sung-Heung;Kyung, Jung-Soo;Yoon, Ho-Seok;Kim, Jae-Yoo;Yoo, Ik-Je;Ham, Young-Jae;Kim, Seong-Tae;Park, Ki-Moon
    • Journal of Dairy Science and Biotechnology
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    • v.40 no.4
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    • pp.173-181
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    • 2022
  • Industrial-scale Ca-fortified Dangmyon was manufactured with added casein phosphopeptides (CPP) to increase Ca adsorption in the intestine. Very low P content in eggshell Ca (egg Ca) and Dangmyon could make it possible to indirectly measure CPP content in Dangmyon. Partitions of the whole P present in Dangmyon were made into sweet potato, egg Ca, and CPP. The CPP content was obtained by multiplying CPP per P by the amount of P partitioned into CPP. It was found that 88.46% of CPP was obtained. However, when milk Ca, which was much higher in P, was added to fortify Dangmyon with CPP, it was found that the CPP content was either under- or over-estimated. Care must be taken when a much higher content of P as a Ca source is selected using this method. It was discovered that the added Ca and CPP were retained after cooking at boiling temperature; therefore, Dangmyon could be an excellent Ca and CPP carrier for humans.

Physicochemical Characteristics of Sweetpotato (Ipomoea batatas (L.) Lam) Starch Depending on Cultivation Periods (재배기간에 따른 고구마(Ipomoea batatas (L.) Lam) 전분의 이화학적 특성)

  • Han, Seon-Kyeong;Song, Yeon-Sang;Ahn, Seung-Hyun;Yang, Jung-Wook;Lee, Hyeong-Un;Lee, Joon-Seol;Chung, Mi-Nam;Nam, Sang-Sik;Choi, In-Hoo;Park, Keun-Hyung
    • Korean Journal of Food Science and Technology
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    • v.46 no.6
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    • pp.750-756
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    • 2014
  • This study was performed to understand the effect of cultivation periods on the physicochemical characteristics of the starch of five sweetpotato cultivars, cultivated in Muan, Korea. Starch, protein, and ash contents increased with increased cultivation period, whereas amylose content decreased. Rapid viscosity analysis showed that the pasting temperature, peak viscosity, breakdown, setback and final viscosity increased with increased cultivation period. However, trough and final viscosity decreased. Although the onset temperature and peak temperature values increased, the conclusion temperature did not show any consistent patterns by differential scanning calorimetry. X-ray diffraction showed that the starch samples had C-type crystallinity irrespective of the cultivation period and cultivar. The starch granules were dominantly round and oval, or polygonal irrespective the cultivation period. The bigger the particle size was, the longer the cultivation period was.

Studies on $\alpha$-amylase of Bocillus circulans F-2 (Part II) Enzymatic characteristics of the purified $\alpha$-amylase (Bacillus circulans F-2가 생산하는 $\alpha$-amylase에 관한 연구 (제 I I 보) 정제$\alpha$-amylase의 효소적특성)

  • ;Hajime Taniguchi;Yoshiharu Maruyama
    • Microbiology and Biotechnology Letters
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    • v.10 no.2
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    • pp.123-132
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    • 1982
  • These experiments were conducted to investigate the enzymatic characteristics of the purified $\alpha$-amylase (F-2A) of Bacillus circulans F-2 and the digestion rate of various starches. 1. The molecular weight was estimated to be 93000 by SDS-polyacrylamide disc gel electrophoresis. The isoelectric point was about pH 5.0. The optimum pH for the enzyme action was 6.0-6.5 and the stable pH ranged pH 5.5-12.0. The optimum temperature was 6$0^{\circ}C$, and the purified $\alpha$-amylase was stable below 4$0^{\circ}C$. 2. The purified $\alpha$-amylase was activated by Mn$^{++}$ and Co$^{++}$, whereas it was inhibited by Ag$^{+}$, HT$^{++}$, Cu$^{++}$ and Pb$^{++}$. 3. The purified $\alpha$-amylase is considered to have no sulfhydryl residue essential for its catalytic activity. 4. Michaelis constant (Km) was 1.704 mg/$m\ell$. Activation energy between 25-4$0^{\circ}C$ was 12.297 Kcal/mole, and between 40-6$0^{\circ}C$, it was 7.831 Kcal/mole. 5. The hydrolysis product from soluble starch, amylose and amylopectin in the early stage of hydrolysis was G$_{6}$, and as hydrolysis proceeds, G$_4$and G$_2$appeared. 6. Products from each oligosaccarides are as follows: G$_4$longrightarrow G$_2$+ G$_2$,G$_3$ +G$_1$,G$_{5}$longrightarrow G$_4$+G$_1$,G$_{6}$longrightarrowG$_4$+ G$_2$,G$_{7}$ G$_4$,G$_{8}$longrightarrow G$_4$+G$_4$, 7. On raw potato starch, raw sago starch and raw yam starch, the purified enzyme exhibited a remarkably high digestion rate than Porcine pancreatic amylase and Streptococcus bovis amylase.

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Digestibility of Infant Food and its Nutritional Meaning (유아식품(乳兒食品)의 소화율(消化率)과 영양적(營養的) 의의(意義))

  • Hong, Ki-Wook;Yoo, Jai-Ja
    • Journal of Nutrition and Health
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    • v.7 no.1
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    • pp.17-23
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    • 1974
  • Digestibilities of flour of white wheat, barley, potato, sweet potato, are $5{\sim}11$ percent (3hrs.) in unripe starch state and in gelatinization, digestibilities are up to $20{\sim}30$ percent (3hrs.). And in two states, digestibilities of flour of potato and sweet potato are lower than barley and white wheat flour. If it is not gelatinization, digestibilities of biscuit and cracker are much lower than popped cake (29%). In gelatinization, digestibilities of biscuit and cracker $(19{\sim}29%,\;3hrs.)$ are similar to gelatinized flour of materials. Digestibilities of bread and castern is $21{\sim}32$ percent, it is similar to gelatinized flour of materials. And bread is higher than castera. Digestibility of on the marketing weaning food (II) (15%, 3hrs.) is much lower than food (I) (24.1%, 3hrs.) When the date of manufacturing passed $1{\sim}2$ months, digestibility of some cookies have a tendency to low. When mothers choice the infant weaning food, they consider metritional value (42%) and digestibility (27%). Also when they make weaning food at home, they generally use flour of rice (41%) and white wheat (23%). And they think, the rate of digestion is 49% in the rice flour and white wheat (23%). It was sort of cooked of rice (16%) and weaning food on the marketing(13%), which accepts to good digest. Also it was sort of biscuits (44%) which comparatively high choice snack food.

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Effect of Spring Potato Cultivation Period on Growth, Yield and Processing Quality of Autumn Potato Cultivars (봄감자 재배기간이 가을감자 품종의 생육, 수량 및 가공품질에 미치는 영향)

  • Gyu Bin Lee;Jang Gyu Choi;Do Hee Kwon;Jae Youn Yi;Hee Tae Lee;Yong Ik Jin
    • Korean Journal of Plant Resources
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    • v.37 no.2
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    • pp.149-160
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    • 2024
  • In Korea, potatoes have served as a side dish, but their role as snacks, such as chips or French fries, has recently gained traction. While there is a high demand for processing potatoes, there remains a dearth of research on the impact of double cropping, particularly during autumn, on processing quality. Therefore, this study was conducted to determine the effects of different spring potato cultivation periods on growth, yield, and processing quality during autumn cultivation. Following spring planting, harvest was carried out four times: 70 days, 80 days, 90 days, and 100 days. Subsequently, autumn cultivation was carried out in Gangneung and Seocheon regions using these seed potatoes. Results showed an increase in above-ground emergence rate with shorter spring growing period. When seed potatoes with a spring cultivation period of 80 and 90 days were grown in the autumn in the Gangneung region, the stem length was 47.2 to 48.9, which was greater than that of other treatments. However, stem number and leaf color (SPAD) showed no significant differences across cultivation periods. The number of tubers, tuber weight, yield, and marketable yield did not vary significantly with cultivation periods but described clear cultivar dependent differences. The tuber weight of the Saebong cultivar in Gangneung and the Eunseon cultivar in Seocheon was superior. The starch content peaked at 7.9% when seed potatoes grown for 80 days in the spring were harvested after autumn cultivation in the Gangneung region, but there was no significant differences in the Seocheon region. Glucose content showed a clear difference depending on the cultivation period, increasing with longer spring cultivation period during autumn cultivation. In conclusion, as a result of the effect of the spring potato cultivation period on the growth, yield, and processing quality of tubers when cultivating potatoes in double-cropping, the differences depending on the cultivation period were insignificant, while cultivar-based disparities were pronounced. The Eunseon cultivar exhibited robust above-ground growth and yield, while the Saebong cultivar demonstrated excellent processing quality.

Studies on "IKUTON" Fermented Sweet Potato Starch Pulp for Growing-Finnishing Swine Ration (IKUTON" 발효(醱酵) 고구마 전분박(澱粉粕)의 양돈사료(養豚飼料) 대체(代替)에 관(關)한 연구(硏究))

  • Han, Sung-Wook;Chung, Chang-Cho
    • Korean Journal of Agricultural Science
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    • v.3 no.2
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    • pp.192-197
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    • 1976
  • Study was conducted to estimate the nutritional effect of substitution levels of "IKUTON" (R) inocculate fermented sweet potato pulp for the growing-finnishing pig rations. Twenty five Large white X Landrace cross-breed (15 kg). mixed sex were used in group feeding at the substitution levels of 0, 10, 20, 30, and 40%. The results obtained were summarized as follow: Voluntary feed intake of experimental diets was lowest in 40%. No significant difference in voluntary feed intake was found in 10,20, and 30% levels except 40% P<0.05). The feed efficiency was highest in 20%; (3.43), 30%; (3.80), 10%; (3.97), control; (4.53), and 40%; (4.20) respectively. There was tendency toward increasing feed effioiency in 10,20,30% levels than control. However, the lowest feed efficiency was found in 40% level. The weeks required to gain 80 kg of live weight were 12 weeks for 10%, 13 weeks for control. 14 weeks for 20%, and 30%, but in 40% level of substitution, no 80kg live weight was found up to the end of experiment (15 weeks). Pigs in 40% level were gained live weight only 64% of control animals. The digestibility of experimental diets was 75.2-78.5%, and water intake per kg dry matter intake was $1.95-2.01{\ell}$. No significant effects of feed offer interval to the digestibility and water consumption.

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Optimization of finely ground meat ball analogue formulations using proteins and polysaccharides from plant origin (세절형 식물성 미트볼의 저작 특성 모방을 위한 식물성 단백질과 다당류 소재의 배합비 최적화)

  • Lee, Jong-Yeop;Lee, Eun-Jung;Hong, Geun-Pyo
    • Korean Journal of Food Science and Technology
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    • v.54 no.4
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    • pp.431-436
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    • 2022
  • This study optimized the finely ground meat ball analogue formulation by a series of experiments. Replacing isolated soy protein (ISP) to isolated mung bean protein (IMP) in total 27% protein caused an increase in hardness while adhesiveness, springiness, and chewiness were decreased (p<0.05), and the best ISP to IMP ratio was 10:17. When protein content was changed from 19% (w/w) to 31% (w/w) with the best ISP:IMP ratio, adhesiveness was increased with decreasing protein content, but all texture profiles were greatly decreased comparing to control (p<0.05). To modify texture of plant meat ball, potato starch (PS), κ-carrageenan (KC), methyl cellulose (MC), konjac (KJ), and potato protein (PP) were applied in formulation. Finally, KJ and MC were combined with and without PP in formulation. As a result, 0.5% KJ+MC+PP and 1% KJ+MC were best formulation for plant meat ball, nevertheless, highly harder texture than control must be regulated which warranted further exploration.

Cultivation Conditions for Mass Production of an Antagonistic Bacterium Stenotrophomonas maltophilia BW-13 (길항세균 Stenotrophomonas maltophilia BW-13의 대량배양을 위한 최적 배양조건)

  • Park Jong-Young;Kim Han-Woo;Kim Hyun-Ju;Chun Ok-Ju;Jung Soon-Je;Choi Woobong;Lee Seon-Woo;Moon Byung-Ju
    • Research in Plant Disease
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    • v.11 no.2
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    • pp.158-161
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    • 2005
  • Stenotrophomonas maltophilia BW-13 is a potent biocontrol agent to control crisphead lettuce bottom rot caused by Rhizoctonia solani. To define the optimum conditions for the mass production of the S. maltophilia BW-13, we have investigated optimum culture conditions and effects of various carbon sources on the bacterial growth. The optimum initial pH and temperature were determined as pH $6.0\~7.0 and $35^{\circ}C$, respectively. For the selection of effective carbon source for the mass production, we tested the low molecular carbon sources such as sucrose, glucose, lactose, maltose, manose and the high molecular carbon source such as dough conditioner, rice bran, corn starch, sweet potato starch. As the results, the addition of dough conditioner in a basal medium ($1.25\%\;K_{2}HPO_4,\;0.38\%\;KH_{2}PO_4,\;0.01\%\;MgSO_4{\cdot}7H_{2}O,\;0.5\%\;Yeast extract$) was able to achieve higher cell density and the antifungal activity than others. Therefore, the basal medium containing $3\%$ dough conditioner (named as dough conditioner medium) was finally selected the optimized media for the mass production of BW-13 strain.