• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 2003.10a
• /
• pp.89.2-90
• /
• 2003

Potato late blight, caused by Phytophthora infestans, is one of the important diseases in potato cultivation areas. Though the incidence of late blight was depend on the inoculums and climatic condition In each fields, the foliar blight was reached to 100％ under the severe disease pressure condition in 2003. Outbreak of foliar blight was concentrated from May and July and evaluation of ten fungicides to control of late blight was made at Daekwallryoung area in potato fields. Based on the company recommendation, those fungicides were applied by a sprayer at the recommended rates on a weekly application schedule. Effect of ten fungicides on foliar blight was based on area under disease progress curve (AUDPC). Across all fungicides was reduced by 77％ in AUDPC and dimethomorph was reduced by 92％ in AUDPC during the same period, respectively. Those fungicide were inhibited the mycelial growth of isolate with different rate in chemical amended medium and several fungicides were completely limited the growth of isolate.

• Korean Journal of Plant Resources
• /
• v.22 no.6
• /
• pp.483-488
• /
• 2009

The main objective of this breeding program is to develop high yielding, disease resistance and good processing potato cultivar. 'Gui Valley' is a clonal selection resulting from a cross between 'ND2471-8' and 'Cona'. It has medium plant height and light green foliage. 'Gui Valley' has medium flowering habit and light pink flowers. 'Gui Valley' is a medium maturing potato cultivar and tubers are smooth, yellow skin, light yellow flesh, long-oval tuber shape, distinct red eyes with medium depth and medium dormancy. It has high level of tuber uniformity and good keeping quality. 'Gui Valley' demonstrates resistance to potato virus Y (PVY), soft rot, but moderately susceptible to late blight and common scab. It is also resistant to most of the internal and external physiological disorders particularly dehiscence, hollow heart and internal brown spot. The specific gravity of 'Gui Valley' is significantly higher (1.097) than that of 'Shepody' (1.078). 'Gui Valley' has suitable for processing mainly French fries and chips. This cultivar has high level of tuber uniformity and capable of yielding 37.6 $t{\cdot}ha^{-1}$, which is 18.2% higher than the control potato cultivar 'Shepody' under optimum agronomical practices.

• Journal of Microbiology and Biotechnology
• /
• v.26 no.3
• /
• pp.488-492
• /
• 2016

Rhodococcus species have become increasingly important owing to their ability to degrade a wide range of toxic chemicals and produce bioactive compounds. Here, we report isolation of the Rhodococcus sp. KB6, which is a new leaf-inhabiting endophytic bacterium that suppresses black rot disease in sweet potato leaves. We determined the 7.0 Mb draft genome sequence of KB6 and have predicted 19 biosynthetic gene clusters for secondary metabolites, including heterobactins, which are a new class of siderophores. Notably, we showed the first internal colonization of host plants with Rhodococcus sp. KB6 and discuss its potential as a biocontrol agent for sustainable agriculture.

• Research in Plant Disease
• /
• v.9 no.3
• /
• pp.137-144
• /
• 2003

This study was conducted to clarify developmental characteristics of scab in potato fields in Jeju island, Korea from 1995 to 1999. Occurrence of potato scab increased with repeated cultivaton of potato and high soil pH in the fields. Incidence of the disease was high as 54.8％ in the repeatedly cultivated potato fields but relatively low as 20.8~26.3% in the non-cultivated fields and in the fields where barley and Chinese cabbage were formerly cultivated. A total of 66 isolates were obtained from the diseased patato tubers and identified as Streptomyces scabies, S.turgidiscabies and S. acidiscabies. The isolation frequency of each Streptomyces species was 37.7%, 14.8% and 18.0%, respectively. The optimum temperature for mycelial growth of the Streptomyces spp. was $28~30^{\circ}C$, and the optimum pH for that 6~7.

• Mycobiology
• /
• v.42 no.2
• /
• pp.206-209
• /
• 2014

In this study, we identified the causative agent of stem-end rot in potatoes that were grown in Gangwon alpine areas of Korea in 2013. The disease symptoms included appearance of slightly sunken circular lesion with corky rot on the potato surface at the stem-end portion. The fungal species isolated from the infected potatoes were grown on potato dextrose agar and produced white aerial mycelia with dark violet pigments. The conidiophores were branched and monophialidic. The microconidia had ellipsoidal to cylindrical shapes and ranged from $2.6{\sim}11.4{\times}1.9{\sim}3.5{\mu}m$ in size. The macroconidia ranged from $12.7{\sim}24.7{\times}2.7{\sim}3.6{\mu}m$ in size and had slightly curved or fusiform shape with 2 to 5 septate. Chlamydospores ranged from $6.1{\sim}8.1{\times}5.7{\sim}8.3{\mu}m$ in size and were present singly or in pairs. The causal agent of potato stem-end rot was identified as Fusarium oxysporum by morphological characterization and by sequencing the internal transcribed spacer (ITS1 and ITS4) regions of rRNA. Artificial inoculation of the pathogen resulted in development of disease symptoms and the re-isolated pathogen showed characteristics of F. oxysporum. To the best of our knowledge, this is the first study to report that potato stem-end rot is caused by F. oxysporum in Korea.

• Food Science and Biotechnology
• /
• v.16 no.1
• /
• pp.43-48
• /
• 2007

Collagen-induced arthritis (CIA) is a model for some types of human autoimmune rheumatoid arthritis (RA). In this study, we examined whether ethanol extract of potato (Solanum tuberosum) is efficacious against CIA in mice. Potato extracts (100 and 200 mg/kg) were orally administered to DBA/1J mice once daily for 49 day after initial immunization with type II collagen. Clinical assessment of disease and measurement of paw edema were conducted throughout the study. The production of CIA-related rheumatoid factor, anti-type II collagen antibody, and cytokines were examined in DBA/1J mice. Serum levels of AST, ALT, creatinine, and lipids were measured, and antioxidant enzyme activity in the spleen was also determined. The arthritis score and paw edema were markedly suppressed in the groups treated with potato extract. Levels of rheumatoid factor, anti-type II collagen antibody, interleukin (IL)-1, IL-6, LDL-cholesterol, and malondialdehyde in sera were also reduced by potato extract treatment. The activities of glutathione peroxidase and glutathione reductase were increased in the spleens of CIA mice treated with potato extract. These findings suggest that potato extract has suppressive effects on type II collagen-induced arthritis, an animal model for human RA.

• Journal of Plant Biotechnology
• /
• v.47 no.2
• /
• pp.157-163
• /
• 2020

Calmodulin (CaM) mediates cellular Ca²⁺ signals in the defense responses of plants. We previously reported that GmCaM-4 and 5 are involved in salicylic acid-independent activation of disease resistance responses in soybean (Glycine max). Here, we generated a GmCaM-4 cDNA construct under the control of the cauliflower mosaic virus (CaMV) 35S promoter and transformed this construct into potato (Solanum tuberosum L.). The constitutive over-expression of GmCaM-4 in potato induced high-level expression of pathogenesis-related (PR) genes, such as PR-2, PR-3, PR-5, phenylalanine ammonia-lyase (PAL), and proteinase inhibitorII (pinII). In addition, the transgenic potato plants exhibited enhanced resistance against a bacterial pathogen, Erwinia carotovora ssp. Carotovora (ECC), that causes soft rot disease and showed spontaneous lesion phenotypes on their leaves. These results strongly suggest that a CaM protein in soybean, GmCaM-4, plays an important role in the response of potato plants to pathogen defense signaling.

• Korean Journal Plant Pathology
• /
• v.14 no.4
• /
• pp.331-338
• /
• 1998

To develop the model for prediction of potato late blight progress, the relationship between severity index of potato late blight transformed by the logit and Gompit transformation function and cumulative severity value (CSV) processing weather data during growing period in Taegwallyeong alpine area, 1975 to 1992 were examined. When logistic model and Gompertz model were compared by determining goodness of fit for progressive degree of late blight using CSV as independent variable, the coefficients of determination were higher as 0.742 in the logistic model than 0.680 in the Gompertz model. Parameters in logistic model were composed of progressive rate and initial value of logistic model. Initial value was calculated in -3.664. The progressive rate of potato late blight was 0.137 in cv. Superior, 0.136 in cv. Irish Cobbler, and 0.070 in cv. Jopung without fungicide sprays. According to in crease of the number of spray times the progressive rate was lowered, was 0.020 in cv. Superior under the conventional program of fungicide sprays, 10 times sprays during cropping season. Equation of progressive rate, b1=0.0088 ACSV-0.033 (R2=0.976), was written by examining the relationship between the parameters of progressive rate of late blight and the average CSV (ACSV) quantifing weather information. By estimating parameters of logistic function, model able to describe the late blight progress of potato, cv. Superior was formulated in Y=4/(1+39.0·exp((0.0088 ACSV-0.033)·CSV).

• The Plant Pathology Journal
• /
• v.19 no.6
• /
• pp.284-287
• /
• 2003

Potato tubers with scab lesions were found in several regions of Gangwon province in Korea during disease surveys in 2000 and 2001. The lesions appeared as shallow scabs with torn and raised skin around the periphery of the lesion. Sometimes the lesions expanded on tuber surface and formed hollowed-out areas. The causal organism was identified as Spongospora subterranea based on morphological characteristics using light and electron microscope. Ten cultivars of potato were evaluated to select resistant cultivars against powdery scab. Among the 10 cultivars, Jowon, Superior, and Atlantic were resistant, while Sephody, Gawon, and Irish Cobbler were susceptible to powdery scab. In the pathogenicity test, typical symptoms of powdery scab were formed on potato tubers, and galls developed on root hairs. This is the first report of powdery scab of potato caused by S. subterranea in Korea.

• Korean Journal of Plant Tissue Culture
• /
• v.26 no.3
• /
• pp.177-182
• /
• 1999

A fungal infection assay between normal and transgenic potato harboring chitinase gene in cultivar Belchip was investigated. In the first stage of experiment, seven transgenic lines having 12cm tall were tested for their resistance against potato late blight pathogen Phytophthora infestans by infection with the zoospores, artificially, Susceptibility to potato late blight infection could be classified into three types based on the rate. In terms of resistance to the disease, two lines were higher, two lines were more suppressive, and three lines were similar as compared with the control. In the following experiment, only 2 risistant lines and 1 suppressed line were used to confirm the resistance again. The results of both experiments were similar. Furthermore, two highly resistant transgenic lines grown in field exhibited a higher resistance than control under the conditions of natural ocurrence of the fungal disease.