• Title/Summary/Keyword: Potato Disease

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AtCBP63, a Arabidopsis Calmodulin-binding Protein 63, Enhances Disease Resistance Against Soft Rot Disease in Potato (애기장대 칼모듈린 결합 단백질 AtCBP63을 발현시킨 형질전환 감자의 무름병 저항성 증가)

  • Chun, Hyun-Jin;Park, Hyeong-Cheol;Goo, Young-Min;Kim, Tae-Won;Cho, Kwang-Soo;Cho, Hyeon-Seol;Yun, Dae-Jin;Chung, Woo-Sik;Lee, Shin-Woo
    • Journal of Plant Biotechnology
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    • v.38 no.1
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    • pp.62-68
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    • 2011
  • Calmodulin (CaM), a $Ca^{2+}$ binding protein in eukaryotes, mediates cellular $Ca^{2+}$ signals in response to a variety of biotic and abiotic external stimuli. The $Ca^{2+}$-bound CaM transduces signals by modulating the activities of numerous CaM-binding proteins. As a CaM binding protein, AtCBP63 ($\b{A}$rabidopsis thaliana $\b{C}$aM-binding protein $\underline{63}$ kD) has been known to be positively involved in plant defense signaling pathway. To investigate the pathogen resistance function of AtCBP63 in potato, we constructed transgenic potato (Solanum tuberosum L.) plants constitutively overexpressing AtCBP63 under the control of cauliflower mosaic virus (CaMV) 35S promoter. The overexpression of the AtCBP63 in potato plants resulted in the high level induction of pathogenesis-related (PR) genes such as PR-2, PR-3 and PR-5. In addition, the AtCBP63 transgenic potato showed significantly enhanced resistance against a pathogen causing bacterial soft rot, Erwinia carotovora ssp. Carotovora (ECC). These results suggest that a CaM binding protein from Arabidopsis, AtCBP63, plays a positive role in pathogen resistance in potato.

Application of Chitosan Preparations for Eco-friendly Control of Potato Late Blight (감자 역병의 친환경 방제를 위한 키토산 제형의 살포)

  • Chang, Taehyun;Kim, Byung Sup
    • Research in Plant Disease
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    • v.18 no.4
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    • pp.338-348
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    • 2012
  • Potato late blight caused by Phytophthora infestans Cooke is one of the major diseases in the cultivation of potatoes in Korea. Effect of chitosan preparations (SH-1 and SH-2) was evaluated on the inhibition of mycelial growth of P. infestans, and protective activity using detached potato leaf assay both in vivo and in the field test. SH-1 and SH-2 were showed protective activity of young plant with control values more than 95% potato late blight by inoculation with pathogens under growth chamber conditions. Mycelial growth was inhibited the radial growth over 74% at a concentration of $300{\mu}g/ml$ of both SH-1 and SH-2. Spraying with SH-1 and SH-2 on the leaves for detached leaf assay reduced disease development. The content of total polyphenol in stem was significantly increased by SH-1 and SH-2 application in the field. In field experiments, foliar application with both SH-1 and SH-2 were significantly reduced the development of late blight on potato plants. Control of late blight disease was obtained with control values of 72% and 53% by application of 1% SH-1 and SH-2, respectively, with 4 times at 7 days interval, and reduced with similar disease control values by application with 3 times at 14 days interval compared with untreated control. SH-1 and SH-2 applications increased the fresh weight of potato, and higher grade potatoes were also increased. The results showed that SH-1 and SH-2 applications can be used as eco-friendly natural fungicide for organic farming for the increase of yields and control of late blight.

A Detailed Review on Recognition of Plant Disease Using Intelligent Image Retrieval Techniques

  • Gulbir Singh;Kuldeep Kumar Yogi
    • International Journal of Computer Science & Network Security
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    • v.23 no.9
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    • pp.77-90
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    • 2023
  • Today, crops face many characteristics/diseases. Insect damage is one of the main characteristics/diseases. Insecticides are not always effective because they can be toxic to some birds. It will also disrupt the natural food chain for animals. A common practice of plant scientists is to visually assess plant damage (leaves, stems) due to disease based on the percentage of disease. Plants suffer from various diseases at any stage of their development. For farmers and agricultural professionals, disease management is a critical issue that requires immediate attention. It requires urgent diagnosis and preventive measures to maintain quality and minimize losses. Many researchers have provided plant disease detection techniques to support rapid disease diagnosis. In this review paper, we mainly focus on artificial intelligence (AI) technology, image processing technology (IP), deep learning technology (DL), vector machine (SVM) technology, the network Convergent neuronal (CNN) content Detailed description of the identification of different types of diseases in tomato and potato plants based on image retrieval technology (CBIR). It also includes the various types of diseases that typically exist in tomato and potato. Content-based Image Retrieval (CBIR) technologies should be used as a supplementary tool to enhance search accuracy by encouraging you to access collections of extra knowledge so that it can be useful. CBIR systems mainly use colour, form, and texture as core features, such that they work on the first level of the lowest level. This is the most sophisticated methods used to diagnose diseases of tomato plants.

Occurrence of Potato Witches' Broom Caused by a Phytoplasma in Korea (파이토플라스마에 의한 감자빗자루병 발생)

  • 함영일;류경열;조일찬
    • Research in Plant Disease
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    • v.7 no.2
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    • pp.116-119
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    • 2001
  • Witches' broom symptoms were firstly found on tubers of Solanum tuberosum cv, Deijima, showing dense growth of spindly sprouts in Cheju province, Korea. Plantlets from the diseased plants also produced the typical witches' broom symptoms, having densely-growing small leaves when they became adult plants. At the later stages the diseased leaves were blightened. Presence of phytoplasma in plant tissues was confirmed by DAPI-staining fluorescence microscopy and electron microscopy, exhibiting its localization in sieve tubes of stem, petiole, and midrib. This is the first report of potato witches' broom in Korea.

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Seasonal Incidence of Potato virus f Infection on Potato Cultivars for the Double Crops in Korea (2기작 감자 품종의 재배 시기별 PVY 감염 정도 조사)

  • Hahm Young-Il;Lee Young-Gyu
    • Research in Plant Disease
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    • v.12 no.1
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    • pp.28-31
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    • 2006
  • One of major potato viruses is Potato virus Y (PVY) in Korea. In the southern part of Korea, potatoes have been grown as double crops in a year by using cv. 'Dejima' and 'Chubak' due to very short dormancy. However, they have caused a serious problem such as a rapid degeneration. It has been thought that the degeneration is affected by the high incidence of PVY in neighboring potato fields. Therefore, the investigation of factors causing the degeneration is very important in the production of healthy seed potato. In this study, the PVY reinfection rates of several potato varieties and the different seed sources of cv. 'Chubak' have been investigated. Results show that the lowest infection rate of PVY among four potato cultivars derived from minitubers is cv. 'Superior'. The others are in order of 'Dejima', 'Atlantic' and 'Chubak'. Also, the incidences of PVY differ significantly when several seed sources are examined. When the seed potatoes (G2, the progeny of microtuber) as spring potato crops are planted in area without potato field nearby, the infection rate of PVY is as low as that of microtubers. However, PVY incidence in the progenies of minitubers as fall potato crops largely increases. Therefore, the best way of potato production under double cropping system is to use the healthy seed potato produced in area without potato field and plant relatively resistant cultivar such as Dejima.

Development of customized control modules for the model forecasting the occurrence of potato late blight (감자역병 예측모델을 위한 맞춤통보용 방제모듈 개발에 대한 고찰)

  • Shim, Myung Syun;Lim, Jin Hee;Kim, Jeom-Soon;Yoo, Seong Joon
    • Korean Journal of Agricultural Science
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    • v.41 no.1
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    • pp.23-27
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    • 2014
  • Potato late blight occurrence is caused by various environmental factors, and the progress can be regularly predicted so that several predictive models have been developed. The models predict the timing of the disease occurrence, but they do not include the methods of the disease control. Effective fungicide control, economic threshold, prediction models were investigated in the study to reflect on customized control modules for the model forecasting the occurrence of potato late blight.

The Comparison of Resistance of Sweet Potato Cultivars to Sclerotium Rot Caused by Sclerotium rolfsii (품종 간 고구마 흰비단병(Sclerotium rolfsii) 발생 정도 비교)

  • Kim, Shin-Chul;Kim, Ju-Hee;Cheong, Seong-Soo;Choi, Kyu-Hwan;Lee, Wang-Hyu;Shim, Hong-Sik
    • Research in Plant Disease
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    • v.20 no.2
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    • pp.126-128
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    • 2014
  • This study was carried out to assay the disease incidence degree of Sclerotium rot caused by Sclerotium rolfsii in sixteen most popular commercial cultivars of sweet potato (Ipomoea batatas) in Republic of Korea. The degree of disease incidence was evaluated on pot experiments. In pot experiments using artificial inoculation, the Sclerotium rolfsii caused a stem rot on seedling of sweet potato plants and causes a crown rot on lower stems near or at the soil line at favorable environmental conditions. White mycelial mats and sclerotia were formed at the infection sites. Plants severely infected were fell over or died because lower stems near soil surface were rotten. The degree of disease incidence was varied according to cultivars. Two cultivars, Bio-mi and Deayumi, were very resistant, while five cultivars, Shinjami, Shingeonmi, Hongmi, Yeonjami, and Shinhung-3 were highly susceptible.

Resistance Induction and Enhanced Tuber Production by Pre-inoculation with Bacterial Strains in Potato Plants against Phytophthora infestans

  • Kim, Hyo-Jeong;Jeun, Yong-Chull
    • Mycobiology
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    • v.34 no.2
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    • pp.67-72
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    • 2006
  • Efficacy of resistance induction by the bacterial isolates Pseudomonas putida (TRL2-3), Micrococcus luteus (TRK2-2) and Flexibacteraceae bacterium (MRL412), which were isolated from the rhizosphere of plants growing in Jeju Mountain, were tested in a greenhouse. The disease severity caused by Phytophthora infestans was effectively reduced in the potato plants pre-inoculated with bacterial isolates compared with those of the untreated control plants growing in a greenhouse. In order to estimate the level of protection by the bacterial isolates, Mancozeb WP (Diesen $M^{(R)}$, Kyong nong) and DL-3-amino butyric acid (BABA) were pre-treated, whereas Dimethomorph WP ($Forum^{(R)}$, Kyong nong) and phosphonic acid ($H_{3}PO_{3}$) were post-treated the challenge inoculation with the pathogen. Disease severities of chemical pre-treated as well as post-treated plants were reduced compare to those of the untreated. The disease reduction in the plants pre-treated with Mancozeb WP was the highest, whereas that of post-treated with Dimethomorph WP was the lowest. The yields of plants pre-inoculated with three bacterial isolates were greatly increased than those of control plants. These results suggest that biological control by bacterial isolates might be an alternative strategy against late blight disease in potato plants growing in greenhouse.

Elimination of SPFMV from Virus-infected Sweet Potato Plants through Apical Meristem Culture

  • Kim, Young-Seon;Jeong, Jae-Hun;Park, Jong-Suk;Eun, Jong-Seon
    • Plant Resources
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    • v.7 no.3
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    • pp.200-205
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    • 2004
  • Sweet potato infected with a viral disease (SPFMV) showed irregular chlorotic patterns, so called feathering associated with faint or distinct ring spots that have purple-pigmented borders. SPFMV was eliminated from sweet potato plants using meristem tip culture. MS medium supplemented with BAP (2mg/L) and NAA (0.05 mg/L) was used for shoot proliferation and 1/2 MS medium for rooting of the plants. Highest percentage of regenerated plants (60%) was obtained from the optimum size (0.3-0.5mm) meristem tips. Of these, 60% plants were found negative for SPFMV by RT-PCR. Virus detection by RT-PCR was found to be a reliable method. Meristem-tip culture to produce SPFMV-free quality sweet potato and virus detection by RT-PCR is an efficient, time saving and reliable method for production of SPFMV-free tissue culture raised plants.

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