• The Plant Pathology Journal
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• v.35 no.3
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• pp.234-242
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• 2019

Thirty-four strains of bacteria were isolated from Phellodendron amurense. Using Nectria haematococca as an indicator strain, the best strain, B18, was obtained by the growth rate method. The morphological, physiological and biochemical characteristics of strain B18 and its 16S DNA gene sequence were identified, and the biocontrol effect of strain B18 was assessed in pot and field tests, as well as in a field-control test. Drilling methods were used to determine the antibacterial activity of metabolites from strain B18 and their effects on the growth of pathogen mycelia and spores. The best bacteriostatic rate was 85.4%. B18 can hydrolyse starch and oxidize glucose but does not produce gas; a positive result was obtained in a gelatine liquefaction test. According to 16S DNA gene sequencing, strain B18 is Bacillus methylotrophicus (GenBank accession number: MG457759). The results of pot and field-control trials showed 98% disease control when inoculating $10^8cfu/ml$ of the strain. The disease control effect of the B18 culture liquid (concentrations of $10^8$, $2{\times}10^6$, $10^6$, $5{\times}10^5$ and $2.5{\times}10^5cfu/ml$) in the field-control test was higher than 80%, and the cure rate of the original delivery solution was 96%. Therefore, in the practical forestry production, a $2.5{\times}10^5cfu/ml$ culture liquidshould be applied in advance to achieve good control effects.

• Journal of Ginseng Research
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• v.28 no.1
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• pp.67-70
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• 2004

Bacillus subtilis B-4228 selected from ginseng field soil for prevention of rusty root was tested for the control of ginseng root rot. In petri-plate dual culture, mycelial growth of Cylindrocarpon destructans was inhibited by B-4228 and hyphal swelling of C. destructans was occurred. In pot experiment with C. destructans-contaminated soil B-4228 dipping of ginseng seedling showed significant preventive effect of root rot (p=0.01), percent healthy root 82% and 20% for treatment and control, root rot rate 6% and 50.4%, respectively.

• Journal of Microbiology
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• v.43 no.5
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• pp.398-405
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• 2005

Polyamines such as putrescine are small, ubiquitous polycationic molecules that are required for optimal growth of eukaryotic and prokaryotic cells. These molecules have diverse effects on cell physiology and their intracellular content is regulated by de novo synthesis and uptake from the environment. The studies presented here examined the structure of a putative polyamine transporter (Pot) operon in Streptococcus pneumoniae (pneumococcus) and growth of pneumococci in medium containing putrescine substituted for choline. RT-PCR experiments demonstrated that the four genes encoding the Pot system are co-transcribed with murB, a gene involved in an intermediary step of peptidoglycan synthesis. Pneumococci grown in chemically-defined media (CDM) containing putrescine without choline enter logarithmic phase growth after 36-48 hs. However, culture density at stationary phase eventually reaches that of choline-containing medium. Cells grown in CDM-putrescine formed abnormally elongated chains in which the daughter cells failed to separate and the choline-binding protein PspA was no longer cell-associated. Experiments with CDM containing radiolabeled putrescine demonstrated that pneumococci concentrate this polyamine in cell walls. These data suggest that pneumococci can replicate without choline if putrescine is available and this polyamine may substitute for aminoalcohols in the cell wall teichoic acids.

• Korean Journal of Breeding Science
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• v.41 no.3
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• pp.358-362
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• 2009

A New Cymbidium variety "Yellow Evening" was developed from a cross between "Oji" and "Valley Flower Cherry Life" followed by seedling and line selections at the National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA) in 2004. After investigation of the characteristics and selection for 9 years (1996-2004), it has been selected specifically pot orchid flower. "Yellow Evening" has medium plant height and semi-erect peduncle. It has medium flowering habit and brownish yellow flowers (RHS, Y11B: petal and sepal) with grayed purple GP183A) spot on lip, when fully opened. Each peduncle has 15.3 flowers with diameter of about 7.4 cm. It has adequate peduncle height, with some curved and spreading petal and sepals. Under optical culture conditions, it started to bloom early-winter (November).

• The Korean Journal of Malacology
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• v.24 no.2
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• pp.131-136
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• 2008

Growth and survival of the spat of arkshell, Scapharca broughtonii were investigated for the improvement of survival in intermediate culture with different shape of protective net and type of preventive net of spat loss. The growth performance of the spat of arkshell was observed for 60 days by using different forms of protective net, as the exposure form with an average shell length of $12.8{\pm}3.2$ mm(P<0.05), the fish pot form with $12.2{\pm}3.5$ mm, the cylinder form with $11.9{\pm}3.8$ mm and the last one is the rectangular form with $10.9{\pm}3.7$ mm. Their numerical value did not show any marked difference with each other. But in case of survival rate, the fish pot form showed highest survival rate which is 43.7% and the significantly lowest was showed by the exposure form, which is 5.4%(P<0.05). The cylinder form and rectangular form showed 41.2 and 31.6% respectively. And then the growth of the spat of arkshell was observed for 163 days by rearing in a sort of preventive net, the first group was in a blackout curtain with average shell length of $13.9{\pm}3.1$ mm, the next group was in a balsam pear net with $12.9{\pm}3.0$ mm, in a polyethylene net with $11.8{\pm}3.1$ mm and the control with $12.6{\pm}3.3$ mm which was not installed by preventive net of spat loss. The survival rate was 91.5% in a blackout curtain, 90.1% in a polyethylene net, 88.5% in a balsam pear net and 61.5% in a controlled group. It is seen that the highest growth and survival rate were observed in a fish pot form and cylinder form. These results were a little bit difference from those of the spat of arkshell cultured in the form of different preventive nets. There was no significant difference in survival rate in relation to the sorts of preventive net against carrying-away, but there was a difference in survival rate by more than 30% as against the non-installed controlled group. We expect that survival rate would be highly improved in intermediate culture carried out with fish pot form and cylinder form of protective net and preventive net of spat loss.

• Microbiology and Biotechnology Letters
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• v.27 no.1
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• pp.1-7
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• 1999

A fungus, Penicillium sp. PS-113, isolated from soil showed the high phosphate-solubilizing activity in patato dextrose broth-rock phosphate to produce free phosphates to the culture broth with the concentrations of 585 ppm against rock phosphate. In this medium, the optimum temperature and initial pH to solubilize rock phosphate were 30$^{\circ}C$ and pH 7.5, respectively. In order to make the mass production of the conidia from this fungus, we cultured in on various solid-based media like barley, corn, wheat, rice, rice bran, and compost. As a result, the fungus highly produced conidia ranging from 2.1 to $5.1{\times}10_9$ conidia/g${\cdot}$media on these solid media except compost-based medium, which was 0 times less than others. Effects of inoculation of the phosphate solubilizing fungus as a biofertilizer were studied in perlite-based pot cropped with Zea mays Suwon 19. Inoculation of Penicillium sp. PS-113 increased in plant height (1.4 times), plant weight (5.2~8.1 times) and root length (1.1~1.2 times) at 60-day cultivation, compared to Hogland solution either without $NH_4H_2PO_4$ or displace $NH_4H_2PO_4$ to powdered rock phosphate, a phosphorus source for plant growth.

• Journal of Plant Biology
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• v.11 no.3
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• pp.1-7
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• 1968

Organic matter in rice-paddy soils exercises two antagonistic effects on the rice plant under water-logged conditions in growing season in the course of its decomposition: It liberates mineral nutrients and promotes soil fertility. On the other hand, however, it demands oxygen for its decay and therefore competes with rice roots for this element, when applied in large quantity of fresh status. For the practical end of rice culture, it is most desirable that these two effects should not contend with each other. To determine the proper content of organic materials to be applied, the influences of varied amounts of a homogeneous mixture of dried green manure, ranging from 0 to 20g/pot (1/20,000 tanbo), upon hte growth of rice was investigated in a sand culture. Labeled phosphorus fertilizer was also used in the form of KH232PO4 to evaluate the availability of this nutrient in the soil. Under the present experimental conditions, green mature seems to have influenced little on the growth of rice, except on number of grains produced and grains/straw ratio. Moreover, no sympton of growth inhibition is obsrvable even by the largest amount of its application. The available phosphorus, as estimated by A-value, appears to have increased, as the amount of organic materials applied increases. In view of the fact that pure sand instead of a paddy soil is used in this culture, the present results would not be directly applicable to practical rice farming. Besides, the estimated A-value is in need of further study, since it varies according to method of application, as suggested by Nishigki et. al. (1958).

• Journal of the East Asian Society of Dietary Life
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• v.7 no.1
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• pp.97-105
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• 1997

This research follows the previous research on Kimchi Culture for the Koreans in Commonwealth of Independent States(CIS) and the purpose of this research was to investigate the habits with Kimchi of the Koreans in CIS. The research was carried out with items of the preparation and the preservation of Kimchi. The results were summarized as follows : 1. Abut 95% of above 30 years old of the respondents prepared Kimchi for their family. But 51.9% of under 30 years old and the forth generation of the respondents got Kimchi without self preparation. 2. All the respondents argued that the procedure of Kimchi preparation of the present indifferent from that of the past. 3. 40.2% of the respondents used to take Kimchi after 2~3 days from the preparation and 29.1% used to take after 1~2 days. 52.8% of the respondents would like to eat the well-matured Kimchi and 45.2% to eat Kimchi which was immediately prepared. 4. The most popular container for keeping was the enamel pot and the most popular places for keeping was the refrigerator. The popular keeping containers were a wooden box(61.2%), a earthen vessel(20.1%), a plastic vessel(14.7%) and the popular keeping places were a underfloor(37.9%), veranda (25.0%), underearth(22.9%) in order.

• The Korean Journal of Food And Nutrition
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• v.12 no.4
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• pp.387-396
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• 1999

Dog meat was begun to be edible by the Chinese, Japanese, the French, Belginan, German Philippines Vietnamese, North-Americans African-Indians Canadian-aborigines Alaskan aborigines including Kor-eans. According to the record, Korea has a long history to have eaten dog meat from the era of Sam-kug(three kingdoms BC 57∼AC 668) and so there are numerous languages proverbs, and customs re-lated to the dog meat. Over the long history there have been many records and recipes about the edib-leness of dog meat. But at present time only the way of cooking such as Bosintang(a soup) Suyuk(a boiled meat) Duruchighi(boiled meat added spice and slightly roasted) Muchim(boiled meat added by spice and mixed) Gaesoju(an extract) Jeongol(boiled meat mixed with spices vegetables and water on the pot) remains. Koreans eat dog meat following the traditional customs n the Boknal(hottest day in summer). Also the areas of Buyo. Sochon, Boryong adn Chongyang of Chungnam province and the ad-jacent areas like Kongju, Iksan, and Nonsan have customs to kill the dog and offer dog meat to the gue-sts in time of small or big occasions such as funeral ceremony Hoigap(anniversary of one's 60th birth-day) and one's birthday. This range of customs is expanding larger and larger. These areas are the cen-ter of past Baekche(BC 57∼AC 660). In spite of this it is unreasonable, and excessive action for foreig-ner to fine fault with the dog meat or Korean food culture.

• Applied Biological Chemistry
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• v.11
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• pp.173-184
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• 1969

To elucidate the effect of Amo-1618(4-hydroxyl-5-isopropyl-2-methlphenyl trimethyl ammonium chloride, 1-piperidine carboxylate) known as a kind of growth retardant, on the growth, grain yield, increasing the efficiency of nitrogen fertilizer, behavior of mineral nutritions and the rate of phosphorus utilization, this experiment was conducted pot culture method in a vinyl house. Two nitrogen level, namely, practical nitrogen level(1 N) and three times nitrogen level(3 N) was made and labelled double-calcium-superphosphate $Ca(H_2P^{32}O_4)_2.\;2H_2O)$ as a source of radioactive phosphorus(P-32) was employed $80\;{\mu}c/pot$, respectively. Rice seedlings, variety 'Suwon No. 82', was transplanted to a 1/50,000 a china pot on June 13 in 1968. For treatment, at early stage of tillering, 10,000 ppm solution of Amo-1618 was foliar sprayed only one time. The Duncan's new mutiple-range test was adopted for statistical analysis evaluating experimental data at 5% level significance. The results obtained may be summarized as follows; 1) No significant difference was found among the treatments in plant height, but in plot of Amo-1618 treatment and 3 N level, number of tillers was significantly increased than that of others. 2) Weight of 10,000 kernels and seed-setting rate was also remarkably increased in same treatment above. 3) Grain yield per pot was significantly increased in Amo-1618 and 3 N level application. This results seemed to be due to the increased the factors on the yield. 4) Contents of nitrogen and phosphorus per cent in the grain was likewise increased in Amo-1618 and 3 N application. There is, however, no difference among treatments in the content of nitrogen and phosphorus in the leaves and culms of rice plants. 5) On the other hand, the contents of potassium and magnesium, no distinctly tendeny showed among treatments. 6) The rate of phosphorus utilization was significantly increased in the plot of Amo-1618 and 3 N application.