• Title/Summary/Keyword: Pot culture

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Changes of Ascorbic Acid and Nitrate Content in Lettuce by Unbalanced Nutrient Solution

  • Park, Yang-Ho;Park, So-Hyeon;Park, Jae-Hong;Lee, Ju-Young;Jang, Byoung-Choon;Lee, Ki-Sang
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.1
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    • pp.107-111
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    • 2006
  • This study was to verity that the uptake inhibition and accumulation of nitrogen in different potassium levels. Lettuce was used as model plant in this study and grown in pot of 10cm's in diameter and depth with mixture media of vermiculite and perlite under supply of different culture solution for three weeks. Nitrogen absorption at root was inhibited by increased potassium concentration in nutrient solution, and nitrate accumulation of plant was depended on absorption of nitrogen because nitrate content of 0 K level was 4-5 times higher than that of 2 K level, Concentration of ascorbic acid was decreased by increasing the nitrogen absorption, since ascorbic acid (AsA) content of 2K level was higher than those of OK level in both of old leaf and flesh leaf.

An Evaluation Study on Total Nitrogen(T-N) Item of Agricultural Water Standards (농업용수 수질기준 T-N 항목에 대한 검증 실험( I ))

  • Choi, Seon-Hwa;Kim, Ho-Il;Kim, Min-Ho;Lee, Byeon-U;Lee, Bong-Hun
    • Journal of The Korean Society of Agricultural Engineers
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    • v.46 no.1
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    • pp.99-105
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    • 2004
  • The present agricultural water quality standards are set by a policy goal. This is intended for water quality management of public water resources, but not for the use of water resources. These standards were not determined by considering the influence of water quality on the safety of agricultural produce and the growth, yield and quality of agricultural crops. Thus, this study was carried out to investigate the influence of irrigation water quality on the growth, yield, and grain quality of rice and acquire fundamental knowledges to set up irrigation water quality standards. The pot experiment was conducted with 4 treatments using irrigation waters with various total nitrogen concentrations (control, 1, 5, 10, 20mg/L) and replicated four times with randomized block design. The results of this study showed that plant height, number of tiller, plant dry weight, the uptake of N, P, and K, and rice protein contents tended to increase as the T-N concentration in irrigation water was increased. In addition, grain yield at T-N 20 mg/L was significantly higher than in the control, but the percentage of head rice was slightly lower due to the increase of green kernel and white belly/core kernel.

Endophytic Bacillus subtilis MJMP2 from Kimchi inhibits Xanthomonas oryzae pv. oryzae, the pathogen of Rice bacterial blight disease

  • Cheng, Jinhua;Jaiswal, Kumar Sagar;Yang, Seung Hwan;Suh, Joo-Won
    • Journal of Applied Biological Chemistry
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    • v.59 no.2
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    • pp.149-154
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    • 2016
  • An endophytic bacterial strain was isolated from kimchi, a Korean traditional fermented Brassica campestris and identified as Bacillus subtilis MJMP2 based on the 16S rRNA sequence. This strain showed strong antagonistic activity against Xanthomonas oryzae pv. oryzae (Xoo) KACC10331, the pathogen of bacterial rice blight disease, as well as activity against some other rice phytopathogenic fungi. The active compound was purified through size-exclusion chromatography and preparative High-performance liquid chromatography. The molecular weight was determined as m/z 1043 by mass spectroscopy, which is identical to that of iturin A. Furthermore, a crude extract from the culture supernatant of Bacillus subtilis MJMP2 showed inhibitory activity against rice blight disease in both a rice leaf explant assay and a pot assay. The crude extract also enhanced the length of roots of Arabidopsis thaliana. These results suggest that the strain Bacillus subtilis MJMP2 could be used as a biological agent to control rice blight disease.

A Study on the Appraisal of Appropriate Planning in Public-Toilet of Amusement Park in Kangwon-Do (강원지역 주요 유원지 공중화장실의 적정설계 평가에 관한 연구)

  • Kim Eun-Joong
    • Korean Institute of Interior Design Journal
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    • v.14 no.4 s.51
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    • pp.62-69
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    • 2005
  • This study shows the present conditions and the appraisal of Public-Toilet in ammusement park of Kangwon-Do. The areas of Public-Toilets are various from over $30m^2\;to\;230m^2$ and the construction costs are also various. The exterior designs aim at Provincial characteristics, traditional images, or modem minimal styles. The floor plans are usually symmetric forms by rectangular or circular spaces. There are some laws and manuals from related association to regulate Public-Toilet. By appraising with these laws and manuals we can notice that there are many problems to improve in Public-Toilet. As the survey, we can find that the unpreparedness of the cognition equipment about whether someone uses the public-toilet has most frequency in related laws. And by the laws about multipurpose toilet, the insufficiency of the accessing space to the chamber pot has most frequency. By the manual of related association, there are much more indications - for example, the unpreparedness of wind protection room or etiquette bell, so on - than by related laws. For the improvement of the culture In Public-Toilet, we should not only improve the related laws, but also activate the deliberation about the appropriate planning in Public-Toilet.

Control of Fungal Diseases with Antagonistic Bacteria, Bacillus sp. AC-1

  • Park, Yong-Chul-
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 1994.06a
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    • pp.50-61
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    • 1994
  • Biological control of important fungal diseases such as Phytophthora blight of red pepper, gary mold rot of vegetables, and powdery mildew of many crops was attempted using an antagonistic bacterium, Bacillus sp. AC-1 in greenhouses and fields. The antagonistic bacterium isolated from the rhizosphere soils of healthy red pepper plant was very effective in the inhibition of mycelial growth of plant pathogenic fungi in vitro including Phytophthora capsici, Rhizoctonia solani, Pyricularia oryzae, Botrytis cinerea, Valsa mali, Fusarium oxysporum, Pythium ultimum, Alternari mali, Helminthosporium oryzae, and Colletotrichum gloeosporioides. Culture filtrate of antagonistic Bacillus sp. AC-1 applied to pot soils infested with Phytophthora capsici suppressed the disease occurrence better than metalaxyl application did until 37 days after treatment in greenhouse tests. Treatments of the bacterial suspension on red pepper plants also reduced the incidence of Phytophthora blight in greenhouse tests. In farmers' commercial production fields, however, the controlling efficacy of the antagonistic bacteria was variable depending on field locations. Gray mold rot of chinese chives and lettuce caused by Botrytis cinerea was also controlled effectively in field tests by the application of Bacillus sp. AC-1 with control values of 79.7% and 72.8%, respectively. Spraying of the bacterial suspension inhibited development of powdery mildew of many crops such as cucumber, tobacco, melon, and rose effectively in greenhouse and field tests. The control efficacy of the bacterial suspension was almost same as that of Fenarimol used as a chemical standard. Further experiments for developing a commercial product from the antagonistic bacteria and for elucidating antagonistic mechanism against plant pathogenic fungi are in progress.

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The Paddling and Round Pots (타날문단경호(打捺文短頸壺)의 연구(硏究))

  • Seong-Ju, Lee
    • Korean Journal of Heritage: History & Science
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    • v.33
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    • pp.4-35
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    • 2000
  • The paddling technique is a Kind of secondary treatments in the process of ceramic forming, which appeared in the Chinese Neolithic Age pottery making. In the case of Korean prehistoric pottery making, it was first introduced together with the kiln firing method from Tongpei region(東北地方) of China in Yan(燕) dynasty occupation period. Korean archaeologists have recognized the adoption of the new technologies as a drastic innovation of ceramic production. And most of them have thought that the diffusion of new techniques, accompanied by the migration of the northern ethnic groups, had been immediately followed by the innovative changes in pottery procdution. However, rejecting the arguments from the simple diffusionist viewpoint, I have first tried to describe the innovation processes in the ceramic production systems as a spatio-temporal process. The paddling technique by the cord-wound paddle, which was first introduced among the various paddles, was associated with the new sort of pottery, round pots fired in low temperature of reducing atmosphere condition. The cord-marked round pots first tried by the indigenous potters in the southern part of Korean were characterized by the relatively low leveled techniques in forming and firing, compared to those of north-east China. The techniques of the round pots were hardly improved in the domestic production system until the appearance of the fully-specialized one. The specialized production system of the round pot, which appeared first in the mid-western region of Korea, showed the diversified paddling techniques and made the noticeable improvements in forming and firing processes.

Evaluation of Streptomyces saraciticas as Soil Amendments for Controlling Soil-Borne Plant Pathogens

  • Wu, Pei-Hsuan;Tsay, Tung-Tsuan;Chen, Peichen
    • The Plant Pathology Journal
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    • v.37 no.6
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    • pp.596-606
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    • 2021
  • Soil-borne diseases are the major problems in mono cropping. A mixture (designated LTM-m) composed of agricultural wastes and a beneficial microorganism Streptomyces saraceticus SS31 was used as soil amendments to evaluate its efficacy for managing Rhizoctonia solani and root knot nematode (Meloidogyne incognita). In vitro antagonistic assays revealed that SS31 spore suspensions and culture broths effectively suppressed the growth of R. solani, reduced nematode egg hatching, and increased juvenile mortality. Assays using two Petri dishes revealed that LTM-m produced volatile compounds to inhibit the growth of R. solani and cause mortality to the root knot nematode eggs and juveniles. Pot and greenhouse tests showed that application of 0.08% LTM-m could achieve a great reduction of both diseases and significantly increase plant fresh weight. Greenhouse trials revealed that application of LTM-m could change soil properties, including soil pH value, electric conductivity, and soil organic matter. Our results indicate that application of LTM-m bio-organic amendments could effectively manage soil-borne pathogens.

Screening of Antagonistic Bacillus against Brown Rot in Dendrocalamus latiflorus and Preparation of Applying Bacterial Suspension

  • Fengying Luo;Hang Chen;Wenjian Wei;Han Liu;Youzhong Chen;Shujiang Li
    • The Plant Pathology Journal
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    • v.40 no.1
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    • pp.1-15
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    • 2024
  • The aim of this study was to isolate biocontrol bacteria that could antagonize brown rot of Dendrocalamus latiflorus, optimize the culture conditions, and develop an effective biocontrol preparation for brown rot of D. latiflorus. This study isolated a bacterium with an antagonistic effect on bamboo brown rot from healthy D. latiflorus rhizosphere soil. Morphology, molecular biology, and physiological biochemistry methods identified it as Bacillus siamensis. The following culturing media and conditions improved the inhibition effect of B. siamensis: the best culturing media were 2% sucrose, 1.5% yeast extract, and 0.7% potassium chloride; the optimal culturing time, temperature, pH, and inoculation amount were 48 h, 30℃, 6, and 20%. The optimum formula of the applying bacterial suspension was 14% sodium dodecyl benzene sulfonate emulsifier, 4% Na2HPO4·2H2O, 0.3% hydroxypropyl methylcellulose thickener, and 20% B. siamensis. The pot experiment results showed the control effect of applying bacterial suspension, diluted 1,000 times is still better than that of 24% fenbuconazole suspension. The applying bacterial suspension enables reliable control of brown rot in D. latiflorus.

Biocontrol of Damping-Off(Rhizoctonia solani) in Cucumber by Trichoderma asperellum T-5 (Trichoderma asperellum T-5를 이용한 오이 모잘록병(Rhizoctonia solani)의 생물학적 제어)

  • Ryu, Ji-Yeon;Jin, Rong-De;Kim, Yong-Woong;Lee, Hyang-Burm;Kim, Kil-Yong
    • Korean Journal of Soil Science and Fertilizer
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    • v.39 no.4
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    • pp.185-194
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    • 2006
  • A fungal strain of Trichoderma having strong chitinolytic activity was isolated from field soil enriched with crabshell for several years. Based on 5.8S rRNA, partial 18S, 28S rRNA genes, ITS1, ITS2 sequence analysis and morphological characteristics, the fungus was identified as Trichoderma asperellum and named as Trichoderma asperellum T-5 (TaT-5). The fungus released lytic enzymes such as chitinase and ${\beta}$-1, 3-glucanse, and produced six antifungal substances in chitin broth medium. To demonstrate the protective effect of TaT-5 against damping-off in cucumber plant caused by Rhizoctonia solani, TaT-5 culture broth (TA), chitin medium (CM) and distilled water (DW) were applied to each pot at 10 days after sowing, respectively. Then, the homogenized hyphae of R. solani were infected to each pot at 1 week after TaT-5 inoculation. During experimental period, fresh weight of shoot and root in cucumber plant more increased at TA treatment compared to other treatments. PR-proteins (${\beta}$-1, 3-glucanase and chitinase) activities in cucumber leaves markedly increased at CM and DW treatments, but the activity slightly increased and then decreased at TA treatment at 3 days after infection of R. solani. The activity of PR-proteins activities in cucumber roots at all treatments decreased with time where the degree of decrement was more alleviated at TA treatment than CM and DW. These results suggest that the lytic enzymes (chitinase and ${\beta}$-1, 3-glucanse) and antifungal substances produced by TaT-5 can reduce the pathogenic attack by R. solani in cucumber plants.

Butachlor and Silicate Fertilizer Interaction to Rice (Butachlor와 규산질비료(珪酸質肥料)의 수도(水稻)에 대(對)한 상호작용(相互作用))

  • Oh, B.Y.;Ryang, H.S.;Shin, Y.H.
    • Korean Journal of Weed Science
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    • v.4 no.1
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    • pp.52-61
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    • 1984
  • Pot and laboratory tests were undertaken to investigate the influence of silicate fertilization on butachlor phytotoxicity to rice. Growth of rice seedlings at 150 ppm of $SiO_2$ was stimulated, while adverse effect was observed over 300 ppm of $SiO_2$ and growth reduction was enhanced with combination of butachlor and $SiO_2$ Rice growth in pot trial at 150kg/10a of silicate fertilization was not influenced by recommended amounts of butachlor and nitrofen, however, the growth of Seokwang byeo at 300kg/10a of silicate was markedly retarded by butachlor in the initial stage of growth. Growth reduction of Seokwang byeo caused by combined application of silicate and butachlor was recovered 50 days after herbicide application. Growth reduction from butachlor was not influenced by pH level and also degradation behaviors of butachlor in submerged soil was not altered by silicate fertilization. Adsorbed amount of butachlor on rice root was increased with addition of $SiO_2$ and its amount in Seokwang byeo was higher than that of Jinju byeo. Butachlor absorption by Seokwang byeo was accelerated by 150 ppm of $SiO_2$ applied simultaneously, but those effect was not encountered in Jinju byeo. Butachlor absorption of rice seedlings was also increased by 150 ppm of $K_2O$, while CaO hindered the absorption and $Na_2O$ had no effect on the absorption. Residual level of butachlor in Seokwang byeo treated with combined solution of butachlor and $SiO_2$ was continued higher than that with butachlor alone during 10 days after transplantation to culture solution.

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