• Structural Engineering and Mechanics
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• v.65 no.4
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• pp.477-490
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• 2018

Reinforced concrete (RC) columns, as the primary load-bearing structural components in buildings, may need to be strengthened due to material deteriorations, changes in usage, new building codes or new design requirements. The use of post compressed plates (PCP) to strengthen existing RC columns has been proven experimentally and practically to be effective in solving stress-lagging effects between the original column and the new strengthening jacket caused by the pre-existing loads. This paper presents a comprehensive summary and review of PCP strengthening techniques to strengthen preloaded RC columns. The failure mode, deformability, and ductility of the strengthened RC columns are reviewed.

• Steel and Composite Structures
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• v.19 no.5
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• pp.1237-1258
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• 2015

This paper proposes a simple inelastic analysis approach to efficiently map out the complete nonlinear post-collapse (strain-softening) response and the maximum load capacity of axially loaded concrete encased steel composite columns (stub and slender). The scheme simultaneously incorporates the influences of difficult instabilizing phenomena such as concrete confinement, initial geometric imperfection, geometric nonlinearity, buckling of reinforcement bars and local buckling of structural steel, on the overall behavior of the composite columns. The proposed numerical method adopts fiber element discretization and an iterative M${\ddot{u}}$ller's algorithm with an additional adaptive technique that robustly yields solution convergence. The accuracy of the proposed analysis scheme is validated through comparisons with various available experimental benchmarks. Finally, a parametric study of various key parameters on the overall behaviors of the composite columns is conducted.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.6
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• pp.1109-1115
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• 1997

Hydrocarbons were analyzed in irradiated beef, pork, dried and seasoned beef, dried anchovy, dried squid, dried shrimp, and fish paste to determine whether the hydrocarbons can be used as markers for detecting post-irradiation of the imported meat and fish products. The samples were irradiated at 0.5, 1, 3, and 6 kGy. Fat was extracted with hexane, and hydrocarbons were separated from the fat through Florisil column. The hydrocarbons were analyzed with GC. Hydrocarbons 15:0, 16:1, 17:1, 16:2, 17:2, and 16:3 in beef and pork, 17:1, 16:2, and 17:2 in dried and seasoned beef, 16:2 in dried anchovy, 16:1 and 17:1 in dried squid, 16:1, 17:1, and 16:2 in dried shrimp, and 16:1, 16:2, and 16:3 in fish paste were detected in the irradiated samples, but not in the unirradiated, so that the hydrocarbons may be used as makers for detecting post-irradiation of each item.

• Bulletin of the Korean Chemical Society
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• v.34 no.12
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• pp.3817-3824
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• 2013

Various separation modes in HPLC, such as anion exchange (AE), reversed-phase (RP), and affinity (AF) chromatography were examined for the separation of selenium species in human blood serum and urine. While RP- and AE-HPLC were mainly used for the separation of small molecular selenium species, double column AF-HPLC achieved the separation of selenoproteins in blood serum efficiently. Further, the effluent of AF-HPLC was enzymatically hydrolyzed and then analyzed with RP HPLC for selenoamino acid study. The versatility of the hybrid technique makes the in-depth study of selenium species possible. For quantification, post column isotope dilution (ID) with $^{78}Se$ spike was performed. ORC ICP/MS (octapole reaction cell inductively coupled plasma/mass spectrometry) was used with 4 mL $min^{-1}$ Hydrogen as reaction gas. In urine sample, inorganic selenium and SeCys were identified. In blood serum, selenoproteins GPx, SelP and SeAlb were detected and quantified. The concentration for GPx, SelP and SeAlb was $22.8{\pm}3.4\;ng\;g^{-1}$, $45.2{\pm}1.7\;ng\;g^{-1}$, and $16.1{\pm}2.2\;ng\;g^{-1}$, respectively when $^{80}Se/^{78}Se$ was used. The sum of these selenoproteins ($84.1{\pm}4.4\;ng\;g^{-1}$) agrees well with the total selenium concentration measured with the ID method of $87.0{\pm}3.0\;ng\;g^{-1}$. Enzymatic hydrolysis of each selenium proteins revealed that SeCys is the major amino acid for all three proteins and SeMet is contained in SeAlb only.

• Journal of environmental and Sanitary engineering
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• v.23 no.4
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• pp.37-44
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• 2008

In this study we developed the analytical methods for the determination of three neurotoxin; anatoxin-a, saxitoxin and neosaxitoxin using HPLC/FLD system and this analytical methods were applied to real sample; algae culture and algae extracts. For the HPLC/FLD analysis of anatoxin-a samples were concentrated on WCX(Weak Cation Exchanger) SPE and then anatoxin-a in concentrate was derivatized with NBD-F solution. Supernatant was injected on HPLC system. For the HPLC/FLD analysis of saxitoxin and neosaxitoxin samples were separated on the column and then derivatizied by post column reactor for fluorescen detection. For post column reaction of saxitoxin we feed two kinds of reaction solution; Oxidizing Reagent of which composition was periodic acid(7mM) in 50mM potassium phosphate buffer, pH 9 and acidifying reagent of which Composition was 0.5M acetic acid. The LOD value for anatoxin-a, saxitoxin and neosaxitoxin in HPLC/FLD method was 24.3 ng. $35{\mu}g/L$, $27{\mu}g/L$ respectively. We determined the anatoxin-a content of lyophilized anabaena flos-aquae and $20{\mu}g/g$ d.w. of anatoxin-a was detected. We analyzed saxitoxin and neosaxitoxin in algae culture media and extracts of lypopyllized algal cell cultured and that of Deachung reservior. Saxitoxin and neosaxitoxin in real sample were below the limit of detection. Although there are various water treatment processes for removing neurotoxins were suggested no process give simultaneous and complete removal of neurotoxins. It was cocluded that nanofiltration which reject material by size can be a process for removal of neurotoxins.

• Journal of the Korea Concrete Institute
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• v.22 no.4
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• pp.441-450
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• 2010

This is a preliminary study on the development of precast beam-column joints for dry construction methodology. Precast beam column joints with thru-connectors (BCJ_TC) using high strength bars or PS strands were developed and their seismic performance including strength degradation, stiffness degradation and energy dissipation capacity was experimentally evaluated. Test results showed that compressive failures at the end blocks of PC beam members occurred dominantly while PC columns including panel zones were free from any damage. However, the connections confined with CFRP at the end block showed much improved seismic performance than that of the unconfined connections. Connections with neoprene pad fillers between beam and column interfaces were better than the other connections in all the seismic performances except initial stiffness. To improve the seismic performances of BCJ_TC, compressive strength of the concrete at the end block need to be increased to compensate for the additional compressive stresses due to unbonded connectors and deformation of connectors should be controlled respectively.

• Korean Journal of Food Science and Technology
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• v.45 no.2
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• pp.241-247
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• 2013

AOAC Mouse Bioassay Analysis (MBA) has been the gold standard for the analysis of paralytic shellfish poisoning toxin (PSP toxin) for more than 50 years. However, this method has inaccurate limit of quantification and cannot be used to determine toxic profiles. An HPLC method (PCOX) was optimized for Korean shellfish to establish an alternative or supplementary method for PSP analysis and was intended to be used for the official monitoring and regulation of food. The recovery rate of the PCOX method was 83.5-112.1% and the limit of quantification for total toxin was about $8.6{\mu}g$/100 g. A long-term comparison study showed a good correlation of the PCOX results with the AOAC MBA results: the correlation factors were 0.9534 and 0.9109 for oyster and mussel matrices, respectively. The PCOX method may be used as an alternative or supplementary method for AOAC MBA to monitor the occurrence of PSP and to analyze PSP toxin profile in oysters and mussels.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.31 no.3
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• pp.473-480
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• 2021

To achieve the high-speed implementation of post-quantum cryptography, primitive operations should be tailored to the architecture of the target processor. In this paper, we present the optimized implementation of multiplier operation on RISC-V processor for post-quantum cryptography. Particularly, the column-wise multiplication algorithm is optimized with the primitive instruction of RISC-V processor, which improved the performance of 256-bit and 512-bit multiplication by 19% and 8% than previous works, respectively. Lastly, we suggest the instruction extension for the high-speed multiplication on the RISC-V processor.

• Journal of Korean Society of Steel Construction
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• v.18 no.4
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• pp.491-502
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• 2006

This paper summarizes full-scale test results on CFT column-to- flat plate connections has gained wide acceptance subjected to gravity loading. CFT construction has gained wide acceptance in a relatively short time in domestic building construction practice due to its various structural and construction advantages. However, efficient details for CFT column to flat plate connections have not been proposed yet. Based on the strategies that maximize economical field construction, several connecting schemes were proposed and tested. Test results showed strength and connection stiffness exceeding those of R/C flat p late counterparts. A semi-analytical procedure is presented to model the behavior of CFT column-to-flat plate connections. The five parameters to model elastic to post-punching catenary action range are calibrated based on the limited test data of t to progressive collapse prevention design is also illustrated.

• YAKHAK HOEJI
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• v.59 no.3
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• pp.113-119
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• 2015

Protein phosphorylation is one of most important post-translational modifications (PTMs) and plays an important role in regulation of protein function. Here we develop a method for a global identification of phosphopeptides and phosphorylation sites using nano-LC MS/MS. We compared two separation methods, C4 and strong cation ion exchange (SCX). Before phosphopeptides enrichment with $TiO_2$, total proteins from Rat 1 cells have been separated using C4 column or tryptic peptides of proteins from the cells have been separated using SCX column. Finally, we have detected 52 phosphorylation sites on 41 proteins from SCX method and 375 phosphorylation sites on 252 proteins from C4 method, and determined the function and localization of identified phosphoproteins using DAVID software. In particular, we showed new phosphorylation sites from membrane proteins related to various cell signaling mechanisms. This method may contribute to study global signal networks induced by various signals including ligands and drugs.