• Journal of the Korea Concrete Institute
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• v.21 no.1
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• pp.105-116
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• 2009

Reinforced concrete (RC) structures have been most widely used because of their good economic efficiency. However, it is very weak in tensile stresses and difficult to control deflection due to the heavy self-weight of concrete. On the other hand, it is generally known that prestressed concrete structures can be the most effective to overcome the demerits of RC structures by using various tendon lay-out and its amount. In the prestressed concrete members, the inflection points of tendons should be placed effectively for the deflection control and the moment reduction. Therefore, in this study, the equations of tendon profiles are derived in terms of polynomials that satisfy essential conditions of tendon geometries such as inflection points and natural curved shapes of tendons placed in continuous members, from which vertical components of prestressing forces can be also calculated. The derived high order polynomial expression for the distributed shape of the upward and downward forces was transformed to an simplified equivalent uniform vertical force in order to improve the applicability in the calculation of member deflection. The influences of vertical forces by tendons to deflection and moment in a continuous slab were also considered depending on the distance from column face to the location of tendons. The applicability of the proposed method was examined by an example of deflection calculation for the cases of slabs with and without tendons, and the efficiency of deflection control by tendons was also quantitatively estimated.

• Journal of the Korea Concrete Institute
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• v.24 no.3
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• pp.285-292
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• 2012

Fixed connection is generally used for beam and column connections of concrete structures, but significant damages at the connection due to severe earthquakes have been reported. In order to reduce damages of the connection and improve seismic performance of the connection, several innovative connections have been suggested. One newly proposed connection type allows a rotation of the connection for applications in rotating or rocking beams, columns, and shear walls. Such structural elements would provide a nonlinear lateral force-displacement response since their contact depth developed during rotation is gradually reduced and the stress across the sections of the elements is non-linearly distributed around a contact area, which is called an elastic hinge region in the present study. The purpose of the present study is to define the elastic hinge region or length for the rocking columns, through investigating the cross-sectional stress distribution during their lateral behavior. Performing a finite element analysis (FEA), several parameters are considered including axial load levels (5% and 10% of nominal strength), different boundary conditions (confined-ends and cantilever types), and slenderness ratios (length/depth = 5, 7, 10). The FEA results showed that the elastic hinge length does not directly depend on the parameters considered, but it is governed by a contact depth only. The elastic hinge length started to develop after an opening state and increased non-linearly until a rocking point(pre-rocking). However, the length did not increase any more after the rocking point (post-rocking) and remained as a constant value. Half space model predicting the elastic hinge length is adapted and the results are compared with the numerical results.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.46 no.6
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• pp.28-37
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• 2009

In this paper, we propose an obstacle localization method using a road feature based on a V-disparity map binarized by a maximum frequency value. In a conventional method, the detection performance is severely affected by the size, number and type of obstacles. It's especially difficult to extract a large obstacle or a continuous obstacle like a median strip. So we use a road feature as a new decision standard to localize obstacles irrespective of external environments. A road feature is proper to be a new decision standard because it keeps its rough feature very well in V-disparity under environments where many obstacles exist. And first of all, we create a binary V-disparity map using a maximum frequency value to extract a road feature easily. And then we compare the binary V-disparity map with a median value to remove noises. Finally, we use a linear interpolation for rows which have no value. Comparing this road feature with each column value in disparity map, we can localize obstacles robustly. We also propose a post-processing technique to remove noises made in obstacle localization stage. The results in real road tests show that the proposed algorithm has a better performance than a conventional method.

• Journal of Microbiology and Biotechnology
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• v.16 no.10
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• pp.1529-1536
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• 2006

We cloned a gene of ompH(D:4) from pigs infected with P. multocida D:4 in Korea [16]. The gene is composed of 1,026 nucleotides coding 342 amino acids (aa) with a signal peptide of 20 aa (GenBank accession number AY603962). In this study, we analyzed the ability of the ompH(D:4) to induce protective immunity against a wild-type challenge in mice. To determine appropriate epitope(s) of the gene, one full and three different types of truncated genes of the ompH(D:4) were constructed by PCR using pET32a or pRSET B as vectors. They were named ompH(D:4)-F (1,026 bp [1-1026] encoding 342 aa), ompH(D:4)-t1 (693 bp [55-747] encoding 231 aa), ompH(D:4)-t2 (561 bp [187-747] encoding 187 aa), and ompH(D:4)-t3 (540 bp [487-1026] encoding 180 aa), respectively. The genes were successfully expressed in Escherichia coli BL21(DE3). Their gene products, polypeptides, OmpH(D:4)-F, -t1, -t2, and -t3, were purified individually using nickel-nitrilotriacetic acid (Ni-NTA) affinity column chromatography. Their $M_rs$ were determined to be 54.6, 29, 24, and 23.2 kDa, respectively, using SDS-PAGE. Antisera against the four kinds of polypeptides were generated in mice for protective immunity analyses. Some $50{\mu}g$ of the four kinds of polypeptides were individually provided intraperitoneally with mice (n=20) as immunogens. The titer of post-immunized antiserum revealed that it grew remarkably compared with pre-antiserum. The lethal dose of the wild-type pathogen was determined at $10{\mu}l$ of live P. multocida D:4 through direct intraperitoneal (IP) injection, into post-immune mice (n=5, three times). Some thirty days later, the lethal dose ($10{\mu}l$) of live pathogen was challenged into the immunized mouse groups [OmpH(D:4)-F, -t1, -t2, and -t3; n=20 each, two times] as well as positive and negative control groups. As compared within samples, the OmpH(D:4)-F-immunized groups showed lower immune ability than the OmpH(D:4)-t1, -t2, and -t3. The results show that the truncated-OmpH(D:4)-t1, -t2, and -t3 can be used for an effective vaccine candidate against swine atrophic rhinitis caused by pathogenic P. multocida (D:4) isolated in Korea.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.17 no.4
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• pp.266-274
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• 2024

In this paper, we designed a low-area 32-bit PF (Poly-fuse) OTP IP, a non-volatile memory that stores data required for analog circuit trimming and calibration. Since one OTP cell is constructed using two PFs in one select transistor, a 1cell-2bit multibit PF OTP cell that can program 2bits of data is proposed. The bitcell size of the proposed 1cell-2bit PF OTP cell is 1/2 of 12.69㎛ × 3.48㎛ (=44.161㎛2), reducing the cell area by 33% compared to that of the existing PF OTP cell. In addition, in this paper, a new 1 row × 32 column cell array circuit and core circuit (WL driving circuit, BL driving circuit, BL switch circuit, and DL sense amplifier circuit) are proposed to meet the operation of the proposed multbit cell. The layout size of the 32bit OTP IP using the proposed multibit cell is 238.47㎛ × 156.52㎛ (=0.0373㎛2) is reduced by about 33% compared that of the existing 32bit PF OTP IP using a single bitcell, which is 386.87㎛ × 144.87㎛ (=0.056㎛2). The 32-bit PF OTP IP, designed with 10 years of data retention time in mind, is designed with a minimum programmed PF sensing resistance of 10.5㏀ in the detection read mode and of 5.3 ㏀ in the read mode, respectively, as a result of post-layout simulation of the test chip.

• Journal of Korean Society of Steel Construction
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• v.20 no.5
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• pp.617-624
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• 2008

Well-designed steel moment connections will undergo local buckling before they exhaust their available rotation capacity, and inelastic post-buckling deformation plays a major role in defining the connection rotation capacity. An approximate analytical method to model strength degradation and failure of beam plastic hinges due to local buckling and estimation of the seismic rotation capacity of fully restrained beam-column connections in special steel moment-resisting frames under both monotonic and cyclic loading conditions is proposed in this study. This method is based on the plastic mechanism and a yield line plastic hinge (YLPH) model whose geometry is determined using the shapes of the buckled plastic hinges observed in experiments. The proposed YLPH model was developed for the improved WUF-W and RBS connections and validated in comparison with experimental data. The effects of the beam section geometric parameters on the rotation capacity were discussed in the companion paper (parametric studies).

• Journal of Korean Society of Steel Construction
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• v.21 no.2
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• pp.155-164
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• 2009

In this study, a computationally efficient parallel axial-flexural plastic hinge model is proposed for nonlinear dynamic progressive collapse analysis of welded steel moment frames. To this end, post-yield flexural behavior and the interaction of bending moment and axial force of the double-span beams in the column's missing event was first investigated by using material and geometric nonlinear parametric finite element analysis. A piece-wise linear parallel point hinge model that captures the moment-axial tension interaction was then proposed and applied to nonlinear dynamic progressive collapse analysis of welded steel moment frames with the use of the OpenSees Program. The accuracy as well as the efficiency of the proposed model was verified based on the inelastic dynamic finite element analysis results. The importance of including the catenary action effects for proper progressive collapse resistant analysis and design was also emphasized.

• Journal of Korean Neurosurgical Society
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• v.41 no.2
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• pp.77-81
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• 2007

Objective : In a variety of thoracolumbar diseases, corpectomy followed by interbody bone graft and anterior instrumentation has allowed direct neural decompression and reconstruction of the weight-bearing column by short segments fusion. In this study, we compared spinal stability of the two different anterior thoracolumbar instruments : Z-plate and Kaneda device representing plate and two-rods type, respectively. Methods : A retrospective review was performed for all the patients with thoracolumbar diseases or traumas treated with anterior corpectomy, autologous iliac bone graft, and fixation with instruments from 1996 to 2000. For the anterior instrumentation, Z-plate or Kaneda device was used for 24 [M:F=5:9, average age=37] and 12 [M:F=9:3, average age=41] patients, respectively. The plain AP and lateral flexion-extension films were taken immediately after surgery and at each follow-up. The sagittal and coronal Cobb's angles at the operation segments were used to observe the change of initial fixation status. The surgical time length and bleeding amount of the two groups were compared. Intra-operative and post-operative instrument associated complications were evaluated. Student t-test was used for statistical analysis and p-value less than 0.05 was considered to be significant. Results : Mean follow-up durations for Z-plate and Kaneda device were 24 and 21 months, respectively. The fusion rate was 91% for Z-plate and 100% for Kaneda device. Two cases of Z-plate group showed instrumentation failure during the follow up period, in which additional surgery was necessary. The mean differences of sagittal Cobb's angles among the AP images immediate after surgery and at follow-up were 7 and 2 degrees for Z-plate and Kaneda device, respectively [p<0.05]. The mean differences of coronal Cobb's angles were 5 and 2 degrees for Z-plate and Kaneda device, respectively [p<0.05]. No Intra-operative complication has occurred in both groups. There was no difference in surgery time and bleeding amount between two groups. Conclusion : We think that Kaneda device [rod type] is stronger than Z-plate [plate type] to keep the spinal stability after anterior thoracolumbar surgery.

• Journal of Microbiology and Biotechnology
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• v.18 no.6
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• pp.1081-1089
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• 2008

A novel ginsenoside-hydrolyzing ${\beta}$-glucosidase was purified from Paecilomyces Bainier sp. 229 by a combination of Q-Sepharose FF, phenyl-Sepharose CL-4B, and CHT ceramic hydroxyapatite column chromatography. The purified enzyme was a monomeric protein with a molecular mass estimated to be 115 kDa. The optimal enzyme activity was observed at pH 3.5 and $60^{\circ}C$. It was highly stable within pH 3-9 and at temperatures lower than $55^{\circ}C$. The enzyme was specific to ${\beta}$-glucoside. The order of enzyme activities against different types of ${\beta}$-glucosidic linkages was ${\beta}$-(1-6)>${\beta}$-(1-2)>${\beta}$-(1-4). The enzyme converted ginsenoside Rb1 to CK specifically and efficiently. An 84.3% amount of ginsenoside Rb1, with an initial concentration of 2 mM, was converted into CK in 24 h by the enzyme at $45^{\circ}C$ and pH 3.5. The hydrolysis pathway of ginsenoside Rb1 by the enzyme was $Rb1{\to}Rd{\to}F2{\to}CK$. Five tryptic peptide fragments of the enzyme were identified by a newly developed de novo sequencing method of post-source decay (PSD) matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. By comparing the five identified peptide sequences with the NCBI database, this purified ${\beta}$-glucosidase proves to be a new protein that has not been reported before.

• Analytical Science and Technology
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• v.33 no.1
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• pp.42-48
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• 2020

Bromate is a disinfection by-product generated mainly from the oxidation of bromide during the ozonation and disinfection process in order to remove pathogenic microorganism of drinking water, and classified as a possible human carcinogen by International Agency for Research of Cancer (IARC) and World Health Organization (WHO). For the purpose of determining the trace level concentration of bromate, several sensitive techniques are applied mostly based on suppressed conductivity detection and UV/Visible detection after postcolumn reaction (PCR). In this study, the suppressed conductivity detection method and the PCR-UV/Visible detection method through the triiodide reaction were compared to analyze the trace bromate in water samples and estimated for the availability of these analytical methods. In addtion, the state-of-the-art techniques was applied for the determination of trace level bromate in various water matrices, i.e., soft drinking water, hard drinking water, mineral water, swimming pool water, and raw water. In comparison of two analytical methods, it was found that the conductivity detection had the suitable advantage to simultaneously analyze bromate and inorganic anions, however, the bromate might not be precisely quantified due to the matrix effect especially by chloride ion. On the other hand, the trace bromate was analyzed effectively by the method of PCR-UV/Visible detection through triiodide reaction to satisfactorily minimize the matrix interference of chloride ion in various water samples, showing the good linearity and reproducibility. Furthermore, the method detection limit (MDL) and recovery were 0.161 ㎍/L and 101.0-108.1 %, respectively, with a better availability compared to conductivity detection.