• Journal of Life Science
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• v.30 no.11
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• pp.939-946
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• 2020

Stroke is one of the leading causes of neurological disability worldwide and stroke patients exhibit a range of motor, cognitive, and psychiatric impairments. GPR88 is an orphan G protein-coupled receptor (GPCR) that is highly expressed in striatal medium spiny neurons; its deletion results in poor motor coordination and motor learning. There are currently no studies on the involvement of GPR88 in stroke or in post-stroke brain function recovery. In this study, we found a decrease in GPR88 protein and mRNA expression levels in an ischemic mouse model using Western blot and real-time PCR, respectively. In addition, we observed that, among the three types of cells derived from the brain (brain microvascular endothelial cells, BV2 microglial cells, and HT22 hippocampal neuronal cells), the expression of GPR88 was highest in HT22 neuronal cells, and that GPR88 expression was downregulated in HT22 cells under oxygen-glucose deprivation (OGD) conditions. Moreover, pretreatment with RTI- 13951-33 (10 mg/kg), a brain-penetrant GPR88 agonist, ameliorated brain injury following ischemia, as evidenced by improvements in infarct volume, vestibular-motor function, and neurological score. Collectively, our results suggest that GPR88 could be a potential drug target for the treatment of central nervous system (CNS) diseases, including ischemic stroke.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.3
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• pp.1228-1233
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• 2013

The purposes of this study were to assess the reliability of the Korean version of Montreal Cognitive Assessment (MoCA-K) and Korean version of Mini-Mental State Examination (MMSE-K) and correlation between MoCA-K and MMSE-K to detect cognitive impairment following stroke determined by a neuropsychological battery. Fifty-two chronic stroke patients participated in this study. They divided two group, normal cognitive group (20 patients) with stroke and cognitive impairment group with stroke (32 patients), and research period was from October 2th. to November 30th., 2013. The subjects were measured K-MoCA and K-MMSE. Normal cognitive group showed the moderate reliability in K-MoCA and K-MMSE. Cognitive group showed the more moderate relaibility in K-MoCA and K-MMSE. There was positive correlation as the more score of K-MoCA gain, the more score of K-MMSE showed in both group. The results suggest that both clinical measures are moderately sensitive and proper feasibility to chronic post-stroke cognitive impairment. The both clinical tools suggest to use in research and clinical settings by a neuropshchological battery.

• Journal of Korea Entertainment Industry Association
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• v.14 no.8
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• pp.437-445
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• 2020

The purpose of this study was to examine the effects of whole-body vibration exercise for life care on balance and walking ability in the fall risk stroke patients. 30 stroke patients were divided into two groups, Otago exercise group(n=15) and whole-body vibration exercise group(n=15). Walking ability were measured by 10m walking and Tinetti mobility test with stroke patients and balance ability were measured by BBS and TUG on comparative analysis of pre, post exercise. To compare differences before and after the program, a paired t-test was used and to compare differences between both groups, an independent samples t-test was used. As a result of the test, it was discovered that BBS and Tinetti mobility test of the both groups were increased statistically significantly and TUG and 10m walking test of the both groups were decreased statistically significantly. balance ability and walking ability of the whole-body vibration exercise group were improved statistically significantly in comparison with those of the Otago exercise group. The results of this study showed that whole-body vibration exercise for the improvement of life care had significant effects on improving BBS, TUG, 10m walking and Tinetti mobility test of fall risk stroke patients. Therefore, it is recommended to apply whole-body vibration exercise to improve life-care through improving balance ability and walking ability of fall risk stroke patients.

• Journal of Korean Neurosurgical Society
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• v.67 no.3
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• pp.333-344
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• 2024

Objective : Markers of neuroinflammation during ischemic stroke are well characterized, but additional markers of neural damage are lacking. The study identified associations of behavioral disorders after stroke with histologic neural damage and molecular biological change. Methods : Eight-week-old, 25 g male mice of the C57BL/6J strain were subjected to middle cerebral artery occlusion (MCAO) to induce ischemic stroke. The control group was a healthy wild type (WT), and the experimental group were designed as a low severity MCAO1 and a high severity MCAO2 based on post-stroke neurological scoring. All groups underwent behavioral tests, realtime polymerase chain reaction, triphenyltetrazolium chloride (TTC) staining and Hematoxylin and Eosin staining. One-way analysis of variance was used to analyze statistical significance between groups. Results : In TTC staining, MCAO1 showed 29.02% and MCAO2 showed 38.94% infarct volume (p<0.0001). The pro-inflammatory cytokine interleukin (IL)-1β was most highly expressed in MCAO2 (WT 0.44 vs. MCAO1 2.69 vs. MCAO2 5.02, p<0.0001). From the distance to target in the Barnes maze test, WT had a distance of 178 cm, MCAO1 had a distance of 276 cm, and MCAO2 had a distance of 1051 (p=0.0015). The latency to target was 13.3 seconds for WT, 27.9 seconds for MCAO1, and 87.9 seconds for MCAO2 (p=0.0007). Prospero homeobox 1 (Prox1) was most highly expressed in MCAO2 (p=0.0004). Doublecortin (Dcx) was most highly expressed in MCAO2 (p<0.0001). Conclusion : The study demonstrated that histological damage to neural cells and changes in brain mRNA expression were associated with behavioral impairment after ischemic stroke. Prox1 and Dcx may be biomarkers of neural damage associated with long-term cognitive decline, and increased expression at the mRNA level was consistent with neural damage and long-term cognitive dysfunction.

• 대한예방의학회:학술대회논문집
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• 2002.10a
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• pp.493-494
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• 2002

• International Journal of Contents
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• v.11 no.3
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• pp.7-13
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• 2015

One of the biggest problems of skeletonization is the occurrence of distortions at the junction point of the final binary image. At the junction area, a single point usually becomes a small stroke, and the corresponding trajectory task, as well as the OCR, consequently becomes more complicated. We therefore propose an adaptive post-processing method that uses an adaptive threshold technique to correct the distortions. Our proposed method transforms the distorted segments into a single point so that they are as similar to the original image as possible, and this improves the static handwriting images after the skeletonization process. Further, we attained promising results regarding the usage of the enhanced skeletonized images in other applications, thereby proving the expediency and efficiency of the proposed method.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.1
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• pp.220-226
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• 2012

The purpose of this study was to identify the effect of treadmill gradient training on lower limb muscle activity in chronic stroke patients. The subject were 32 stroke patients.. Subjects were randomly divided into three group which were control group($0^{\circ}$treadmill training(n=10)) and experimental group($5^{\circ}$treadmill training (n=10) and $10^{\circ}$treadmill training(n=12)). Three groups received treadmill gradient training for 30 minutes while 3 times per week for 6 weeks in addition to conventional physical therapy. Muscle strength was measured by EMG on rectus femoris, biceps femoris, tibialis anterior and gastrocnenius for muscle activities. In comparison of activity of rectus femoris and gastrocnemius between pre and post value, the activity of rectus femoris was significant in the experimental and control group(p<.05) and the activity of biceps femoris was significant in the $5^{\circ}$treadmill gait training group and $10^{\circ}$treadmill gait training group(p<.05). The activity of tibialis anterior was significant in the $5^{\circ}$treadmill gait training group and $10^{\circ}$treadmill gait training group(p<.05). In comparison of the difference of activity of rectus femoris among 3 groups, there was a significant difference between the $5^{\circ}$treadmill gait training group and control group(p<.05). and difference of activity of tibialis anterior was significant difference between the $10^{\circ}$treadmill gait training group and control group(p<.05). These findings suggest that $5^{\circ}$treadmill gait training group and $10^{\circ}$treadmill gait training group can be used to improve lower limb muscle activity in chronic stroke patient. In conclusion, these treadmill gradient training helped improving function of gait ability in chronic stroke patient.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.11
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• pp.5730-5736
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• 2013

The study was conducted to determine the effect of trunk pattern training in proprioceptive neuromuscular facilitation(PNF) and weight-shift training on the muscle activity and static balance in patients with hemiplegia due to stroke. Twenty patients with hemiplegia due to stroke were assigned to the trunk pattern training in PNF group(n=10) or weight-shift training group(n=10). Both groups were executed conventional treatment for 5 times per week for 6 weeks 30 minutes per session. Each group performed additional training for 20 minutes. Post training, compared to the weight-shift training group, trunk pattern training in PNF group showed significantly increased on muscle activity of rectus femoris, gastrocnemius and static balance(p<.05). These results support the perceived benefits of trunk pattern training in PNF to augment on the static balance and muscle activity of stroke patients. Therefore, trunk pattern training in PNF is feasible and suitable for stroke patients.

• Journal of Korean Physical Therapy Science
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• v.19 no.4
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• pp.47-52
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• 2012

Purpose : The purpose of the study was to examine if a respiratory muscle strengthening training in patients with stroke can improve their pulmonary function. Methods : Volunteers were included for the study if a patient diagnosed stroke more than 6 months and had 24 points or higher in MMSE-K scores. Twenty-eight subjects participated in this study and were randomly divided into two groups; a breathing exercise group(n=14) and a control group(n=14). The intervention for all subjects was conducted for 20minutes, three times a week for 4 weeks. Subjects for the breathing exercise group had the respiratory muscle strengthening training using spiro-tigers, where-as subjects in the control group got their usual treatment ie a postural training. The six-minute walking test(6MWT) and the pulmonary function tests(FVC, $FEV_1$, $FEV_1$/FVC, VC, Vt, IRV and ERV) were employed to assess treatment effects at baseline and after their intervention. Results : Twenty-four subjects finished their 4-week treatment programs. The general characteristics between groups were found to be similar (p>0.05). The pulmonary function between groups were also observed no difference across groups at the baseline measurement (p>0.05). In the post treatment group comparison, subjects in the breathing exercise group showed an increase in lung function with VC ($2.73{\pm}0.80{\ell}$) and Vt ($0.87{\pm}0.38{\ell}$) than those in the control group ($1.91{\pm}0.80{\ell}$ and $0.48{\pm}0.22{\ell}$ respectively) (p<0.05). However, there was no difference found in 6MWT, FVC, $FEV_1$, $FEV_1$/FVC, IRV, and ERV across groups (p>0.05). Conclusion : A significant increase in VC and Vt was found in subjects with stroke, who had four-week training on respiratory muscle strengthening. However, respiratory muscle strengthening showed no effect on walking speed and FVC, $FEV_1$, $FEV_1$/FVC, IRV, and ERV in patients with stroke.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.6
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• pp.1491-1496
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• 2009

Stroke is usually associated with the cerebral blood flow of the central nervous system. However, studies concerning the effects of neurologic sysmptoms induced from stroke on the peripheral blood flow has not taken place sufficiently. To ascertain the feasibility of a blood flow meter adopting to use doppler ultrasonogrphy, under the prospect that hemiparesis induced from stroke may have effect on the peripheral blood flow, the peripheral blood flow velocity was observed. The control group made up of healthy people without any factors capable of effecting the peripheral blood flow velocity, and patient group which consist of hemiparetic people induced from stroke, were recruited. Volumes of recruitment are 21 persons in the patient group, and 29 persons in the control group, but the final numbers of people are 17 and 21 respectively because of the inconsistancy in the method of the test. The non-invasive method of Doppler effect of Ultrasound was used to measure the blood flow velocity. The blood flow velocity in the peripheral part of left and right fourth fingers:dorsal branches of proper palmar digital artery to dorsum of distal phalanges, was measured in the control group and patient group through Doppler Ultrasound. In comparison of the control group and the patient group, the systolic blood flow velocity from the peripheral part of the upper extremity was lower in the patient group than that of the control group. According to such results, it is concluded that hemiparesis induces the reduction of the peripheral blood flow velocity in the systolic phase.