• Title/Summary/Keyword: Post-Heat treatment

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Inhibitory Effects of a Korean Strain Gpf01 Identified as Pseudomonas fluorescens on Cucumber mosaic virus

  • Ipper, Nagesh S.;Kim, Jung-Eun;Koo, Jun-Hak;Hur, Jang-Hyun;Lim, Chun-Keun
    • The Plant Pathology Journal
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    • v.21 no.3
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    • pp.262-269
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    • 2005
  • An antiviral producing bacterial strain was isolated from a ginseng rhizosphere in Kangwon province of Republic of Korea. In order to identify the bacterial strain, microbiological, physiological and biochemical tests were performed, along with RAPD, 16S rRNA, 16S-23S rRNA ITS (intergenic spacer region) and DNA-DNA hybridization analyses. The bacterium was found to be a strain of Pseudomonas fluorescens, which was designated as Gpf01. The strain was grown in Muller-Hinton (MH) broth, and the culture supernatant obtained was filtered through a $0.45{\mu}l$ filter. It was further boiled at $100^{\circ}C$ and tested in two experiments for its ability to control a yellow strain of Cucumber mosaic virus (CMV-Y). In the first experiment, boiled culture filtrate (RCF) was treated on one half of the leaves of Chenopodium amaranticolor followed by CMV- Y inoculation on both halves. In the second experiment, BCF was treated on the lower leaves of Nicotiana tobacum var. Xanthi-nc, with the CMV-Y mechanically inoculated onto the upper untreated leaves. In the first experiment, BCF treatment was able to considerably reduce the number of viral lesion, and in the second experiment, plants treated with BCF showed no visible viral symptoms compared to the Muller-Hinton (MH) media treated controls 15 days post inoculation (dpi), and remained symptomless throughout the study period. Thus, Gpf01, identified as P. fluorescence, was able to produce an antiviral component in the culture filtrate, which was found to be heat stable, non-phytotoxic and effective in local as well as systemic hosts of CMV.

Specific Capacitance Characteristics of Electric Double Layer Capacitors with Phenol Based Activated Carbon Fiber Electrodes and Aqueous Electrolytes (페놀계 활성탄소섬유 전극과 수용성 전해질을 사용하는 전기이중층 캐패시터의 비축전용량 특성)

  • Kim, Jong Huy;An, Kay Hyeok;Shin, Kyung Hee;Ryoo, Min Woong;Kim, Dong Kuk
    • Applied Chemistry for Engineering
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    • v.10 no.6
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    • pp.814-821
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    • 1999
  • The specific capacitance characteristics of the electric double layer capacitors(ELDC) which were made of phenol based activated carbon fiber(ACF) electrodes. Also the effect of aqueous electrolytes on the cell performance has been investigated with respect to different specific surface areas of electrodes and different kinds of aqueous electrolytes. It has been shown that larger surface area and pore size, higher conductivity of electrodes, and higher ion mobility of electrolytes have better specific capacitances. It has been found that heat treatment at $1200^{\circ}C$ and $CO_2$ post-activation at $900^{\circ}C$ of the electrode are effective to improve the specific capacitance over 145F/g and 165F/g, respectively. The EDLC showed high efficiency and long cycle life over 30000 cycles.

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Effects of composition of preannealed Y-Ba-Cu-0 thin films deposited by sputtering on the superconducting properties and microstructure of post-annealed thin films (스퍼터링 증착된 Y-Ba-Cu-O계 박막의 열처리 전 조성이 열처리 후 박막의 초전도특성 및 미세구조에 미치는 영향)

  • Cho, Hae-Seok;Kim, Hyeong-Jun
    • Korean Journal of Materials Research
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    • v.1 no.1
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    • pp.54-61
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    • 1991
  • YBCO films deposited on MgO(100) and Si(100) by rf-magnetron sputtering using stoichiometric single target were annealed under oxygen atmosphere at $880^{\circ}C$ for 1 hr. The microstructure and superconducting properties of YBCO thin films depended on the composition of pre-annealed thin films. Basal planesuperconducting particles grown on MgO(100) substrate had small and rod-like shade due to preferred orientation, while superconducting film grown on the basal plane-superconducting particles showed round-shape particles. If pre-annealed thin film had nonstoichiometric composition, liquid phase was formed during the heat treatment, which made it easy for particles to grow in the preferred orientation and thus to form textured structure. But the thin films with the textured structure did not show good superconducting properties, especially $T_c$, since the liquid phase transformed into second phase in the grain boundary during the cooling. The effect of the second phase on $T_{c, \;zero}$ was greater than that on $T_{c, \;on}$.

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AES Analysis of Au, Au/Cr, Au/Ni/Cr and Au/Pd/Cr Thin Films by the Change of Substrate Temperature and Annealing Temperature (기판온도와 열처리온도의 변화에 따른 Au/Cr, Au/Ni/Cr 및 Au/Pd/Cr 다층박막의 AES 분석)

  • Yoo, Kwang Soo;Jung, Hyung Jin
    • Analytical Science and Technology
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    • v.6 no.2
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    • pp.217-223
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    • 1993
  • Thin films of the Au/Cr, Au/Ni/Cr and Au/Pd/Cr systems were deposited on alumina substrates at ambient temperature and $250^{\circ}C$ in a high-vacuum resistance heating evaporator and annealed at $300^{\circ}C$, $450^{\circ}C$ and $600^{\circ}C$ for 1 hour in air, respectively. The film thicknesses of Au, Ni(or pd), and Cr were $1000{\AA}$, $300{\AA}$, and $50{\AA}$, respectively. The substrate temperature during deposition and the post-deposition annealing temperature affected the sheet resistance of thin-films due to the inter-diffusion of each layer. As a result of Auger depth profile analysis, in the Au/Cr system Cr already diffused out to Au surface during deposition at the substrate temperature of $250^{\circ}C$ and Au distribution changed after heat treatment. In the Au/Ni/Cr and Au/Pd/Cr systems, diffusion phenomena of Ni and Pd were found and especially Ni (approximately 45 at.%) diffused out to Au surface and oxidized.

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Biological and Physicochemical Properties of Canine Parvovirus lated from the Dogs with Diarrhea (설사증 나환견으로 부터 분리한 Canine Parvovirus의 성상에 관한 연구)

  • 최해연;정운선;전무형;박성국;민원기
    • Korean Journal of Veterinary Service
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    • v.13 no.2
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    • pp.162-183
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    • 1990
  • From 1988 to 1989, 8 strains of canine parvovirus-2 (CPV-2) were isolated from the fecal specimens from the dogs that were clinically diagnosed as canine parvoviral enteritis in the veterinary hospitals located in the regions of Taejon and Chungbuk province. Studios on biological and physicochemical properties for the isolates were carried out. The results obtained by experiments are summarized as follows. 1. Among 62 fecal samples collected from the dogs with enteric diseases, 24 (38.7%) showed the haemagglutinating activity against porcine erythrocyte, ranging from 16 to 16, 384 of HA titers. 2. When 8 fecal specimens with high HA titer over 1, 000 were inocultated into CRFX cells, intranuclear inclusion bodies were obseverd in all of eight specimens, of w)lick three specimens showed cytoplasmic inclusions concurrently with the intranuclear inclusion bodies. 3. In study on species-specificity of haemagglutinating activity of the isolates, TJ-89-1 and TJ-89-2, it was found that the isolates revealed the highest haemagglutinating activity with porcine erythrocytes, showing the relatively lower haemagglutination titers with the erythrocytes from cat and rabbit. None of erythrocytes from other animals reacted with the isolates. 4. By the cross-haemagglutination inhibition test of the Isolates with reference viruses and sera, the Isolates were evidently identified as the strains of canine parvovirus-2. 5. In Physicochemical property test, it was evident that the isolates were stable in, lipid solvent, pH and heat treatment at $56^{\circ}C$ for 30 min. and contain DNA genome. 6. When seven puppies were inoculated intraorally with the isolate at HA titer of 8, 192, all of the puppies showed the symptoms of anorexia, vomiting, diarrhea and died at the 5th to 10th days post inoculation(pi). The fecal samples from all of the puppies revealed significantly high HA titers afterward the 5th days pi. Body temperature and the number of total leucocytes were slightly increased at the early stage of infection. but extremely decreased at the stage of collapse. HI titers of the sera started to increase at the 2nd to 3rd days pi reaching 512 to 1, 024 at the 4th to 5th day pi.

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The Latest Technology Development Trends of Flux Cored Wire (Flux Cored Wire의 최신 기술 개발 동향)

  • Im, Hee-Dae;Choi, Chang-Hyun;Jung, Jae-Heon;Kil, Woong
    • Journal of Welding and Joining
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    • v.34 no.6
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    • pp.1-10
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    • 2016
  • Flux Cored Wire is the most widely used welding material for Flux Cored Arc Welding these days. This paper introduces the technical aspects of manufacturing FCW and the development trend of FCW for each type of steel and metal. The studies are ongoing to lower the production cost of seamless-type FCW since it has not been generally used in welding shops so far because of it high cost even though the seamless-type FCW has various advantages than folded-type FCW in terms of manufacturing technology. Meanwhile, a technical research has been carried out to develop a rutile type of FCW products which satisfies high toughness after post heat treatment. In addition, for high-speed fillet welding, there has been a development of welding materials which can be welded in Single Auto-Carriage 100 cpm or more and up to Twin Tandem 200 cpm without occurring any welding defect in order to improve the welding productivity. As Zn coated steel is being used recently to improve the corrosion resistance of the automotive parts, a research and development for Metal Cored Wire has been conducted to reduce the Si island produced in welding operation than those produced when using the former solid wires. A development of welding material that guarantees CTOD performance beyond $-40^{\circ}C$ CTOD to $-60^{\circ}C$ is underway by different steel grades, and FCW for super austenitic stainless steel is being developed as the corrosion resistant steel has been upgraded.

Analysis of the Corrosion Behavior According to the Characteristics of Sigma Phase Formed in Super Austenitic Stainless Steel (슈퍼 오스테나이트계 스테인리스강의 시그마상 특성에 따른 부식거동 분석)

  • Kim, Ye Eun;Park, Jin-seong;Cho, Dong Min;Hong, Seung Gab;Kim, Sung Jin
    • Corrosion Science and Technology
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    • v.19 no.4
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    • pp.203-210
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    • 2020
  • The corrosion behavior of super austenitic stainless steel was studied by examining the characteristics of the sigma phase formed in the steel. A range of experimental and analytical methods was employed, including potentiodynamic polarization tests, critical pitting temperature tests, transmission electron microscopy, and energy-dispersive spectroscopy. Three steel samples with different sigma phase levels were obtained by intentionally adjusting the manufacturing process. The results showed that the corrosion resistance of the samples was strongly dependent upon the size and distribution of the sigma phase precipitated in the samples. The larger the size of the sigma phase, the higher the Mo content in the sigma phase and the higher the depletion level of Mo at the interface between the matrix/sigma phase, the more samples with a coarse-sized sigma phase were susceptible to localized pitting corrosion at the interface. These results suggest that various manufacturing processes, such as welding and the post-heat treatment of the steel, should be optimized so that both the size and fraction of the sigma phase precipitated in the steel are small to improve the resistance to localized corrosion.

Thermal Process Effects on Grain Size and Orientation in (Bi1La1)4Ti3O12 Thin Film Deposited by Spin-on Method (스핀 코팅법으로 증착한 (Bi1La1)4Ti3O12 박막의 후속 열공정에 따른 입자 크기 및 결정 방향성 변화)

  • Kim, Young-Min;Kim, Nam-Kyeong;Yeom, Seung-Jin;Jang, Gun-Eik;Ryu, Sung-Lim;Sun, Ho-Jung;Kweon, Soon-Yong
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.20 no.7
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    • pp.575-580
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    • 2007
  • A 16 Mb 1T1C FeRAM device was integrated with BLT capacitors. But a lot of cells were failed randomly during the measuring the bit-line signal distribution of each cell. The reason was revealed that the grain size and orientation of the BLT thin film were severely non-uniform. And the grain size and orientation were severely affected by the process conditions of post heat treatment, especially nucleation step. The optimized annealing temperature at the nucleation step was $560^{\circ}C$. The microstructure of the BLT thin film was also varied by the annealing time at the step. The longer process time showed the finer grain size. Therefore, the uniformity of the grain size and orientation could be improved by changing the process conditions of the nucleation step. The FeRAM device without random bit-fail cell was successfully fabricated with the optimized BLT capacitor and the sensing margin in bit-line signal distribution of it was about 340 mV.

A Study on Microstructures and Cryogenic Mechanical Properties of Electron Beam Welds between Cast and Forged Inconel 718 Superalloys for Liquid Rocket Combustion Head (액체로켓 연소기용 Inconel 718 주조 및 단조 합금의 전자빔 용접부 미세조직 및 극저온 특성)

  • Hong, Hyun-Uk;Bae, Sang-Hyun;Kwon, Soon-Il;Lee, Je-Hyun;Do, Jeong-Hyeon;Choi, Baig-Gyu;Kim, In-Soo;Jo, Chang-Yong
    • Journal of Welding and Joining
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    • v.31 no.6
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    • pp.50-57
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    • 2013
  • Characterization of microstructures and cryogenic mechanical properties of electro beam (EB) welds between cast and forged Inconel 718 superalloys has been investigated. Optimal EBW condition was found in the beam current range of 36~39 mA with the constant travel speed of 12 mm/s and arc voltage of 120 kV for 10 mm-thick specimens. Electron beam current lower than 25 mA caused to occur the liquation microfissuring in cast-side heat affected zone (HAZ) of EB welds. The HAZ liquation microfissure was found on the liquated grain boundaries with resolidified ${\gamma}/Laves$ and ${\gamma}/NbC$ eutectic constituents. EBW produced welds showing a fine dendritic structure with relatively discrete Laves phase due to fast cooling rate. After post weld aging treatment, blocky Laves phase and formation of ${\gamma}^{\prime}+{\gamma}^{{\prime}{\prime}}$ strengtheners were observed. Presence of primary strengthener and coarse Laves particles in PWHT weld may cause to reduce micro-plastic zone ahead of a crack, leading to a significant decrease in Charpy impact toughness at $-196^{\circ}C$. Fracture initiation and propagation induced by Charpy impact testing were discussed in terms of the dislocation structures ahead of crack arisen from the fractured Laves phase.

Photocatalytic Properties of WO3 Thin Films Prepared by Electrodeposition Method (전기증착법으로 제조된 WO3 박막의 광촉매 특성)

  • Kang, Kwang-Mo;Jeong, Ji-Hye;Lee, Ga-In;Im, Jae-Min;Cheon, Hyun-Jeong;Kim, Deok-Hyeon;Nah, Yoon-Chae
    • Journal of Powder Materials
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    • v.26 no.1
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    • pp.40-44
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    • 2019
  • Tungsten trioxide ($WO_3$) is a promising candidate as a photocatalyst because of its outstanding electrical and optical properties. In this study, we prepare $WO_3$ thin films by electrodeposition and characterize the photocatalytic degradation of methylene blue using these films. Depending on the voltage conditions (static and pulse), compact and porous $WO_3$ films are fabricated on a transparent ITO/glass substrate. The morphology and crystal structure of electrodeposited $WO_3$ thin films are investigated by scanning electron microscopy, atomic force microscopy, and X-ray diffraction. An application of static voltage during electrodeposition yields a compact layer of $WO_3$, whereas a highly porous morphology with nanoflakes is produced by a pulse voltage process. Compared to the compact film, the porous $WO_3$ thin film shows better photocatalytic activities. Furthermore, a much higher reaction rate of degradation of methylene blue can be achieved after post-annealing of $WO_3$ thin films.