• Speech Sciences
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• v.7 no.3
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• pp.229-247
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• 2000

The purpose of this study is to examine the improvement effect of articulation of the fricative consonant /s/(ㅅ), when applying the Articulation Modification Program (AMP) to hearing disordered students. Three hearing disordered students were selected for this study by the consonant picture and Korean Articulation Sentence tests. They have no defect in their emotion, behavior and sight. This program applied the multiple baseline design across subjects to examine the improvement effect of articulation of fricative consonant /s/(ㅅ). Also I designed th$\sim$s program with an advanced pre-test and post-test in order to research the improvement difference of articulation by articulation position and language unit. It was executed 32 sessions over three months, four sessions a week, one session taking forty minutes. The results according to the study subject are as follow; First, as result of AMP, articulation of all students was developed after applying this program. Second, as result of AMP, articulation in fore-word was one-hundred percent efficiency and articulation in middle-word was 97.7% efficiency by position. Third, as result of AMP, articulation in the word reached 98.7% efficiency, in phrase 97.7% efficiency, in the sentence 97.7% efficiency and in the story was 98.3% efficiency by language unit.

• BMB Reports
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• v.49 no.1
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• pp.45-50
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• 2016

Salsolinol (SAL), a compound derived from dopamine metabolism, is the most probable neurotoxin involved in the pathogenesis of Parkinson's disease (PD). In this study, we investigated the modification and inactivation of human ceruloplasmin (hCP) induced by SAL. Incubation of hCP with SAL increased the protein aggregation and enzyme inactivation in a dose-dependent manner. Reactive oxygen species scavengers and copper chelators inhibited the SAL-mediated hCP modification and inactivation. The formation of dityrosine was detected in SAL-mediated hCP aggregates. Amino acid analysis post the exposure of hCP to SAL revealed that aspartate, histidine, lysine, threonine and tyrosine residues were particularly sensitive. Since hCP is a major copper transport protein, oxidative damage of hCP by SAL may induce perturbation of the copper transport system, which subsequently leads to deleterious conditions in cells. This study of the mechanism by which ceruloplasmin is modified by salsolinol may provide an explanation for the deterioration of organs under neurodegenerative disorders such as PD. [BMB Reports 2016; 49(1): 45-50]

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.12 no.5
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• pp.2348-2365
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• 2018

QR codes as public patent are widely used to acquire the information in various fields. However, it faces security problem when delivering the privacy message by QR code. To overcome this weakness, we propose a secret hiding scheme by improving exploiting modification direction to protect the private message in QR code. The secret messages will be converted into octal digit stream and concealed to the cover QR code by overwriting the cover QR code public message bits. And the private messages can be faithfully decoded using the extraction function. In our secret hiding scheme, the QR code public message still can be fully decoded publicly from the marked QR codes via any standard QR Code reader, which helps to reduce attackers' curiosity. Experiments show that the proposed scheme is feasible, with high secret payload, high security protection level, and resistant to common image post-processing attacks.

• Fashion & Textile Research Journal
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• v.14 no.1
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• pp.64-74
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• 2012

The purpose of this study was to find purchase appraisal standard and post-purchase satisfaction for natural dyeing products. The data used for this study were collected from the women in their from 20's to 50's who are living in the regions of Busan, Ulsan, and Gyeongnam in Korea. The questionnaire of 537 copies was used for a statistical analysis. To analyze data, 2-test, t-test, Cronbach's a test, factor analysis, ANOVA, Duncan test, regression analysis, etc. were carried out. A used statistical package was SPSS 14.0. The analysis results were as follows. The purchase appraisal standards for natural dyeing products were identified as practicality and reliability, suitability of use, symbolic property of status, design property, and convenience of buying. As the analysis result for purchase appraisal standard according to the demographic characteristics, the purchase appraisal standard according to most demographic characteristics showed significant difference. The factors of post-purchase satisfaction for natural dyeing products were verified as product modification and durability, design and price, hygienic property and product quality. As the analysis results of post-purchase satisfaction for natural dyeing products according to demographic characteristics, the post-purchase satisfaction according to demographic characteristics showed significant difference. The factors affecting post-purchase satisfaction were verified as product quality, kinds of item, color change, product lifespan, color change, etc. Consequently, because the purchase appraisal standard of natural dyeing product is very various, it is necessary to enhance post-purchase satisfaction of natural dyeing products by reflecting these factors in advance at the product development stage.

• Journal of Welding and Joining
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• v.33 no.6
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• pp.21-26
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• 2015

Recently, application of UHSS(Ultra High Strength Steels) whose tensile strength is over 1000MPa to car body structure are growing due to great needs for light weighting and improved crash worthiness. However, their poor weldability is one of obstacles to expand selecting to car body. In this study, effect of Phosphorus contents on resistance spot weldability of high elongation DP980 steel whose Si content is over 1% was investigated. The cross tension strength (CTS) was decreased showing partial interface fracture as Phosphorus content increase because of solidification segregation of Phosphorus. In order to improve resistance spot weldability by modification of welding condition, in-situ post-weld heating pulse was introduced after main pulse. The optimum cooling time between main and post pulse and post-pulse current condtion were determined through FEM welding simulation and DOE tests. The CTS was increased about 1.5 time showing plug fracture. The decrease of Phosphorus segregation was found to be a major reason for weld ductility and CTS improvement.

• Journal of Chest Surgery
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• v.33 no.10
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• pp.806-811
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• 2000

Background : The authors have modified the method of Ravitch technique. Material and Method ; This technique was applied to 6 patients out of 18 patients who underwent corrective surgery from May 1987 to July 1999. The technique is quite different from that of Ravitch. We did not divide the intercostal muscle bundles from the laterals of sternum and the Akin's struts were placed retrosternally crossing the chest horizontally to prevent flail motion during immediate post-operative period and retraction of the sternum afterwards. Anterior sternal osteotomy instead of the posterior one was performed for the latest 3 cases which made operative procedure more simple and easy. The struts were removed one year later. Result : Compared to the hospital stay of the patients who received standard Ravitch method that of the six cases who received our modification was definitely shortened from 13.1 days to 8.3 days(p<0.0000). Flail motion was not noted in any patient and chest wall stability was obtained more easily with this technique. Conclusion : Our modification is recommendable for correction of funnel chest in regards to shorter operation time better chest wall stability shorter hospital stay and less complication.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.55-55
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• 1993

Farnesyl Protein transferase(FPT)는 발암유전자 ras의 단백질 산물인 p$^{21}$의 post-translational modification의 첫 단계인 ras-farnesylation에 관여하는 효소로 본 연구에서는 정제된 FPT와 E. coli에서의 발현 system을 이용하여 FPT의 구조와 기능을 밝히고 이를 FPT 방해제의 설계에 이용하고자 한다. Bovine testis에 존재하는 FPT를 30%-50%의 Ammonium sulfate로 fractionation하고, DEAE-Sephacel, Sephacryl S-300 column을 통과시킨 후 peptide(KKCVIM) affinity column을 이용하여 순수 정제하였다. 정제된 효소의 분자량은 gel-filtration에 의해 100KDa으로 추정되었고 SDS-PAGE 결과 49KDa과 46KDa의 두 subunit로 구성되었음이 확인되었다. 효소활성에는 $Mg^{2＋}$ 와 $Zn^{2＋}$가 필수적이며 최적 pH는 7.0이었다. Yeast의 FPT의 두 subunit 유전자는 Yeast genomic DNA를 template로 사용하고 각 subunit에 specific한 합성된 primer들과 vent polymerase를 이용하여 Polymerase chain reaction을 통하여 얻었다. 두 유전자를 pBluescriptII SK＋ vector를 변형시킨 두 vector, pBSK＋4와 pBChl＋4에 재조합 시킨 후 E.coli에 transformation시켜 발현시켰다. 현재 정제된 Bovine FPT와 E. coli에서 발현된 Yeast FPT의 chemical modification과 site-directed mutagenesis를 통하여 FPT의 active site와 substrate binding site에 관한 연구를 진행시키고 있다.

• Journal of Energy Engineering
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• v.28 no.4
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• pp.13-18
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• 2019

The high energy penalty in amine-based post-combustion CO₂ capture process is hampering its industrial scale application. An advanced process is designed by intensive heat integration within the conventional process to reduce the stripper duty. The study presents the technical feasibility for stripper duty reduction by intensive heat integration in CO₂ capture process. A rigorous rate-based model has been used in Aspen Plus® to simulate conventional and advanced process for a 300 MW coal-based power plant. Several design and operational parameters like split ratio, stripper inter-heater location and flowrate were studied to find the optimum values. The results show that advanced configuration with heat integration can reduces the stripper heat by 14%.

• Animal cells and systems
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• v.16 no.1
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• pp.1-10
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• 2012

Most living organisms synchronize their physiological and behavioral activities with the daily changes in the environment using intrinsic time-keeping systems called circadian clocks. In mammals, the key molecular features of the internal clock are transcription- and translational-based negative feedback loops, in which clock-specific transcription factors activate the periodic expression of their own repressors, thereby generating the circadian rhythms. CLOCK and BMAL1, the basic helix-loop-helix (bHLH)/PAS transcription factors, constitute the positive limb of the molecular clock oscillator. Recent investigations have shown that various levels of posttranslational regulation work in concert with CLOCK/BMAL1 in mediating circadian and cellular stimuli to control and reset the circadian rhythmicity. Here we review how the CLOCK and BMAL1 activities are regulated by intracellular distribution, posttranslational modification, and the recruitment of various epigenetic regulators in response to circadian and cellular signaling pathways.

• The Korean Journal of Zoology
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• v.8 no.2
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• pp.33-36
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• 1965

We have carried out a study on the modification of the frequency of X-ray induced lethal and slow growing mutations by colchincine treatment before and after X-ray irradiation in Paramecium aurelia. 1. Lethal and slow-growing mutation induced by X-ray in Paramecium aurelia were reduced by colchicine treatment. 2. The effects of colchicine on the X-ray induced mutations were remarkable in the radiosensitive stages of cell division. 3. The pre-irradiation treatment with colchicine showed no significant influence on the frequency of mutations. 4. It is believed that the reduction of mutation induced by X-ray after treatment with colchicine was due to the fact that the delay of the cell division allowed more time for the pre-mutational damage to recover.