• Journal of Chest Surgery
• /
• v.9 no.1
• /
• pp.102-106
• /
• 1976

The management of infection occurring in the space which remains after removal of lung presents considerable difficulties both for the patient and the surgeon. We have during the past one year, developed a procedure, which has been successfully applied in 4 cases of postpneumonectomy empyema by modification of Clagett's procedure. All their underlying lung diseases were pulmonary tuberculosis. The diagnosis of postpneumonectomy empyema was confirmed by thoracentesis, and bacteriologic cultures were obtained in all patients. Dead space thoracis was irrigated with various kind of antiseptic Solutions and debrided, filled with antibiotics saline solutions. The patients had a smooth post operative course except one case and was discharged from the hospital on the 3 weeks post-operative day in average. The failure case was wound leaking & redeveloped empyema, & waiting for reoperation.

• BMB Reports
• /
• v.47 no.11
• /
• pp.599-608
• /
• 2014

As the prevalence of obesity has increased explosively over the last several decades, associated metabolic disorders, including type 2 diabetes, dyslipidemia, hypertension, and cardiovascular diseases, have been also increased. Thus, new strategies for preventing and treating them are needed. The nuclear peroxisome proliferator-activated receptors (PPARs) are involved fundamentally in regulating energy homeostasis; thus, they have been considered attractive drug targets for addressing metabolic disorders. Among the PPARs, $PPAR{\gamma}$ is a master regulator of gene expression for metabolism, inflammation, and other pathways in many cell types, especially adipocytes. It is a physiological receptor of the potent anti-diabetic drugs of the thiazolidinediones (TZDs) class, including rosiglitazone (Avandia). However, TZDs have undesirable and severe side effects, such as weight gain, fluid retention, and cardiovascular dysfunction. Recently, many reports have suggested that $PPAR{\gamma}$ could be modulated by post-translational modifications (PTMs), and modulation of PTM has been considered as novel approaches for treating metabolic disorders with fewer side effects than the TZDs. In this review, we discuss how PTM of $PPAR{\gamma}$ may be regulated and issues to be considered in making novel anti-diabetic drugs that can modulate the PTM of $PPAR{\gamma}$.

• Food Science and Biotechnology
• /
• v.15 no.6
• /
• pp.902-907
• /
• 2006

Freeze dried crude bacteriocin was encapsulated within an acid-soluble coating material, Eudragit EPO, using a surface modification technique through a hybridization system. The pH and titratable acidity of control yoghurt were 3.92 and 1.56%, respectively, after 24 hr of fermentation at $42^{\circ}C$, whereas yoghurt containing 500 AU/mL encapsulated bacteriocin exhibited a higher pH (4.37) and lower titratable acidity (1.2%). Yoghurt containing encapsulated bacteriocin had significantly lower titratable acidity when the duration of fermentation (to pH 4.5) and subsequent refrigerated storage ($4^{\circ}C$) was longer than 20 days. There were no significant differences in the viability of lactic acid bacteria after 15 hr of fermentation. This suggests that microencapsulated bacteriocin has the potential to control the excessive growth of yoghurt starters caused by temperature abuse or post-acidification.

• Asian-Australasian Journal of Animal Sciences
• /
• v.26 no.5
• /
• pp.609-624
• /
• 2013

The objective of this study was to get a comprehensive understanding of how genes in chicken shell gland modulate eggshell strength at the early stage of active calcification. Four 32-week old of purebred Xianju hens with consistent high or low shell breakage strength were grouped into two pairs. Using Affymetrix Chicken Array, a whole-transcriptome analysis was performed on hen's shell gland at 9 h post oviposition. Gene ontology enrichment analysis for differentially expressed (DE) transcripts was performed using the web-based GOEAST, and the validation of DE-transcripts was tested by qRT-PCR. 1,195 DE-transcripts, corresponding to 941 unique genes were identified in hens with strong eggshell compared to weak shell hens. According to gene ontology annotations, there are 77 DE-transcripts encoding ion transporters and secreted extracellular matrix proteins, and at least 26 DE-transcripts related to carbohydrate metabolism or post-translation glycosylation modification; furthermore, there are 88 signaling DE-transcripts. GO term enrichment analysis suggests that some DE-transcripts mediate reproductive hormones or neurotransmitters to affect eggshell quality through a complex suite of biophysical processes. These results reveal some candidate genes involved with eggshell strength at the early stage of active calcification which may facilitate our understanding of regulating mechanisms of eggshell quality.

• Asian-Australasian Journal of Animal Sciences
• /
• v.22 no.2
• /
• pp.282-288
• /
• 2009

The present paper describes the effects of pressure and post-mortem aging treatments on in situ proteasome activity in rabbit and bovine skeletal muscles. Synthetic peptide hydrolyzing activity of rabbit proteasomes remained in the muscle after exposure to pressures up to 100 MPa. However, when a pressure of 400 MPa or more was applied, proteasomes were markedly inactivated. The extraction of proteasomes from excessively pressurized muscle appeared to be difficult. Proteasomes in aged muscle remained relatively stable throughout the aging process, with activity after 168 h (7 days) being 35%, 48%, 53% and 31% of the 0 h post-mortem LLVY, LSTR, AAF and LLE total hydrolyzing activities, respectively. The synthetic peptide hydrolyzing activities of bovine muscle proteasomes were similar to those of rabbit skeletal muscle proteasomes. The results suggest that synthetic peptide hydrolyzing activity remains in muscle exposed to relatively low pressures. Furthermore, it is known that high-pressure treatment induces fragmentation of myofibrils, modification of actin-myosin interaction and activation of intramuscular proteinases, cathepsins and calpains. Thus, proteasomes are probably involved in the tenderization process in combination with other intramuscular proteinases under high-pressure conditions. Our findings confirmed that proteasomes play a role in meat tenderization induced by high-pressure treatment or aging.

• Korean Journal of Microbiology
• /
• v.37 no.2
• /
• pp.109-113
• /
• 2001

As the complete genomic sequences accumulate, the use of global techniques became possible. DNA microarray is a powerful technology for measuring global mRNA levels. This method, however, does not provide information on post-translational modifications of proteins. In addition, mRNA levels do not strictly correlate with protein concentrations, especially for lower-abundance proteins. Therefore, studies at the level of transcription are not sufficient to understand cellular activity. Proteomic techniques to analyze protein expression and function at the large-scale have been developed and used. This review introduces a simple explanation for proteomic analysis and examples of how proteomics is applied in cell biology.

• Journal of Biomedical Engineering Research
• /
• v.16 no.4
• /
• pp.471-480
• /
• 1995

The modification of Nl -N2 Audiograms before and after loudtone exposure onto the tympanic membrane of guinea pigs was examined. The exposure was a loud continuous tone of 106 dB SPL intensity at 10 KHz for 10 minutes. The comparison between Wl-H2 Audiograms pre and post loudtone exposure indicates that the exposed cochlea permanently lost its normal hearing function. The largest threshold shifts wore found between 12-13.2KHz. The functional change of the cochlea after the loudtone exposure depends on the state of the cochlea before the trauma. More sensitive cochlea is more critically damaged in its function after the trauma than less sensitive cochlea.

• BMB Reports
• /
• v.41 no.8
• /
• pp.587-592
• /
• 2008

Cotesia vestalis is an endoparasitoid of Plutella xylostella larvae and injects a polydnavirus (CvBV) into its host during oviposition. In this report we characterize the gene, CvBV3307, and its products. CvBV3307 is located on segment S33 of the CvBV genome, is 517 bp, and encodes a putative protein of 122 amino acids, including a serine-rich region. The expression pattern of CvBV3307 in parasitized larvae and the subcellular localization of CvBV3307 only in granulocytes indicated that it might be involved in early protection of parasitoid eggs from host cellular encapsulation and in manipulating the hormone titer and developmental rhythm of host larvae. Western blot analysis showed that the size of the immunoreactive protein (about 55 kDa) in parasitized hosts at 48 hours post parasitization (h p.p.) is much larger than the predicted molecular weight of 13.6 kDa, which suggests that CvBV3307 undergoes extensive post-translational modification in hosts.

• Textile Coloration and Finishing
• /
• v.17 no.6 s.85
• /
• pp.27-35
• /
• 2005

Conventional pad- dry-cure(thermo-fixation) process usually produces functional performance on both sides of a fabric. UV curing technique was applied to impart water- and oil-repellent finish effective only on the face of a PET knitted fabric. The preferential one-side coating, by virtue of the limited penetration of UV light, was achieved by W curing after padding of a fluorocarbon agent without special coating or printing equipments. The difference in the functional property of face and back sides was examined by measuring water and oil repellency at each side of the treated fabric. The influence of pre/post-irradiation dose and agent concentration on the performance of the finished fabrics were investigated. While increase in both resin concentration and post-irradiation did not have significant effect on the finish, UV pre-irradiation of PET fabrics caused remarkable influence presumably due to appropriate surface modification of PET fabrics required for facile wetting of the resin. The dimensional stability and color change of the UV cured fabrics measured by FAST and reflectance spectrophotometry showed significantly decreased color difference and increased percent extension compared with the samples pre-irradiated without agent application.

• Journal of the Korean Wood Science and Technology
• /
• v.41 no.2
• /
• pp.123-133
• /
• 2013

This paper presents experimental and numerical tests on a recently developed timber column concealed base joint. This joint was designed to replace the wood-wood connection found in the post-and-beam structure of Hanok, the traditional Korean timber house. The use of metallic connectors provides an increased ductility and energy dissipation for a better performance under reversed loading, especially seismic. In this study, we investigate the performance of the joint under pseudo-static reversed cyclic moment loading through the study of its ductility and energy dissipation. We first perform experimental tests. Results show that the failure occurs in the metallic connector itself because of stress concentrations, while no brittle fracture of wood occur. Subsequent numerical simulations using a refined finite element model confirm these conclusions. Then, using a practical modification of the joint configuration with limited visual impact, we improve the ductility and energy dissipation of the joint while retaining a same level of rotational strength as the originally designed configuration. We conclude that the joint has a satisfying behavior under reversed moment loading for use in earthquake resistant timber structure in low to moderate seismicity areas like Korea.