• Computers and Concrete
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• 제22권6호
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• pp.527-538
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• 2018

Buckling of longitudinal bars is a brittle failure mechanism, often recorded in reinforced concrete (RC) structures after an earthquake. Studies in the literature highlights that it often occurs when steel is in the post elastic range, by inducing a modification of the engineered stress-strain law of steel in compression. A proper evaluation of this effect is of fundamental importance for correctly evaluating capacity and ductility of structures. Significant errors can be obtained in terms of ultimate bending moment and curvature ductility of an RC section if these effects are not accounted, as well as incorrect evaluations are achieved by non-linear static analyses. This paper presents a numerical investigation aiming to evaluate the engineered stress-strain law of reinforcing steel in compression, including second order effects. Non-linear FE analyses are performed under the assumption of local buckling. A role of key parameters is evaluated, making difference between steel with strain hardening or with perfectly plastic behaviour. Comparisons with experimental data available in the literature confirm the accuracy of the achieved results and make it possible to formulate recommendations for design purposes. Finally, comparisons are made with analytical formulations available in the literature and based on obtained results, a modification of the stress-strain law model of Dhakal and Maekawa (2002) is proposed for fitting the numerical predictions.

• Mass Spectrometry Letters
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• 제13권4호
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• pp.139-145
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• 2022

Protein glycosylation is a common post-translational modification by non-template-based biosynthesis. In fungal biotechnology, which has great applications in pharmaceuticals and industries, the importance of research on fungal glycoproteins and glycans is accelerating. In particular, the importance of quantitative analysis of fungal glycans is emerging in research on the production of filamentous fungal proteins by genetic modification. Reliable mass spectrometry-based techniques for quantitative glycomics have evolved into chemical, enzymatic, and metabolic stable isotope labeling methods. In this study, we intend to expand quantitative glycomics by metabolic isotope labeling of glycans in Aspergillus niger, a filamentous fungus model, by the MILPIG method. We demonstrate that incubation of filamentous fungi in a culture medium with carbon-13 labeled glucose (1-¹³C₁) efficiently incorporates carbon-13 into N-linked glycans. In addition, for quantitative validation of this method, light and heavy glycans are mixed 1:1 to show the performance of quantitative analysis of various N-linked glycans simultaneously. We have successfully quantified fungal glycans by MILPIG and expect it to be widely applicable to glycan expression levels under various biological conditions in fungi.

• 생물정신의학
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• 제22권2호
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• pp.55-62
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• 2015

Objectives Social workers can suffer from occupational stress resulting from dealing with clients, which might lead to metal health problems. We aimed to investigate the association of duty-related traumatic experiences and stress coping strategies with post-traumatic stress symptoms among social workers in public sector. Methods A total of 110 social workers in public sector (men 30.9%, $36.5{\pm}7.6$ yrs) participated in this study. All subjects were evaluated the frequency and the impact of duty-related traumatic events. Additionally, they completed questionnaires including the Impact of Event Scale-Revised for post-traumatic stress symptoms, the Beck Depression Inventory-II for depressive symptoms, the Scale for Suicidal Ideation for suicide symptoms and the Ways of Coping Checklist for stress coping strategies. Results The most frequent traumatic events were "Violent or abusive language from a client" (95.0%) and "Client made a fuss" (94.5%). The most distressing traumatic event was "Seeing a dead body on duty" (7.6 out of 10), which predicted post-traumatic stress symptoms (odds ratio 4.04 ; 95% confidence interval, 1.79-9.11). Among 4 types of stress coping strategies, the emotion-focused coping was positively correlated with post-traumatic stress symptoms after controlling age and sex (${\beta}$ = 0.50, p < 0.001). Conclusions Social workers in public sector showed high level of post-traumatic stress symptoms. Duty-related traumatic events and the emotional-focused coping strategies were associated with the severity of post-traumatic stress symptoms. The modification of stress coping strategies would alleviate post-traumatic stress symptoms in social workers in public sector.

• 자연과학논문집
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• 제17권1호
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• pp.13-29
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• 2006

산화-환원 활성 단백질중에 하나인 설피레독신과의 결합 단백질을 효모 Two-hybrid 기법을 이용하여 탐색한 결과, 알파-만노시다제가 설피레독신과 특이적으로 결합함을 밝혔다. 알파-만노시다제는 D-만노스 당을 비환원성 말단으로부터 유리시키는 가수분해 효소로서, 세포 원형질에 다량체 형태로 존재한다. 본 연구에서는 설피레독신과 알파-만노시다제간의 단백질결합을 설피레독신의 새로운 생리기능 관점에서 토의했다.

• 보건교육건강증진학회지
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• 제24권3호
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• pp.1-19
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• 2007

Objectives: The purpose of the this study was to evaluate lifestyle intervention program for the treatment of the metabolic syndrome. Methods: Subjects of this study were 700 adults with metabolic syndrome who took health examinations in health promotion centers of Korea Association of Health Promotion between May 1 and June 30, 2006. Subjects were randomly assigned to an experimental group and a comparison group. Participants in the experimental group received intensive 3-month lifestyle modification intervention and participants in the comparison group received minimal information on lifestyle modification. Pre test and Post test were carried out to evaluate the effectiveness of the intervention program. Results: After the intensive intervention on lifestyle modification(healthy diet, physical activity, moderate drinking, stress management, and smoking cessation), the levels of blood pressure, waist circumference, fasting blood glucose and triglycerides were significantly reduced for those in experimental group(P<0.001). For those in comparison group, the levels of blood pressure(P<0.001), waist circumference(P<0.001), and triglycerides(P<0.01) were significantly reduced after the intervention. No change in the levels of high-density lipoprotein cholesterol were observed in both groups. After 3-month intervention, the prevalence of metabolic syndrome was reduced to 35.7% in experimental group and 48.5% in comparison group. Conclusion: This study has demonstrated the efficacy of therapeutic lifestyle intervention for the management of metabolic syndrome.

• 한국기계가공학회지
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• 제19권8호
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• pp.94-103
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• 2020

This study investigated the effects of ultrasonic nanocrystal surface modification (UNSM) on the deteriorated surface of AISI SUS316L additively manufactured (AM) using the powder bed fusion (PBF) technique. Specifically, the effects of UNSM conditions on surface topology, hardness, and anti-corrosion were examined. Before UNSM treatment, the stainless steel 316L powder was processed via the PBF machine to prepare a substrate. We observed surface changes due to UNSM treatments in PBF SUS316L substrates and examined the correlation between topology changes, roughness, hardness, and anti-corrosion. After UNSM treatment, the coarse as-built surface was refined, and a regular micro-profile was implemented. Compared to the non-treated PBF sample, the waviness and roughness of the surfaces after UNSM treatment decreased by up to 56.0% and 94.5%, respectively, and decreased further as the interval decreased. The hardness improved by up to 63.0% at a maximum depth of 500 ㎛ from top surface by the UNSM treatment. The results of the corrosion test showed that the corrosion resistance of the UNSM specimens was moderately improved compared to that of the untreated surface. This study confirmed that UNSM is an effective post-processing technique for additively manufactured parts.

• Molecules and Cells
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• 제45권6호
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• pp.425-434
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• 2022

The post-translational modification (e.g., phosphorylation) of estrogen receptor α (ERα) plays a role in controlling the expression and subcellular localization of ERα as well as its sensitivity to hormone response. Here, we show that ERα is also modified by UFM1 and this modification (ufmylation) plays a crucial role in promoting the stability and transactivity of ERα, which in turn promotes breast cancer development. The elevation of ufmylation via the knockdown of UFSP2 (the UFM1-deconjugating enzyme in humans) dramatically increases ERα stability by inhibiting ubiquitination. In contrast, ERα stability is decreased by the prevention of ufmylation via the silencing of UBA5 (the UFM1-activating E1 enzyme). Lys171 and Lys180 of ERα were identified as the major UFM1 acceptor sites, and the replacement of both Lys residues by Arg (2KR mutation) markedly reduced ERα stability. Moreover, the 2KR mutation abrogated the 17β-estradiol-induced transactivity of ERα and the expression of its downstream target genes, including pS2, cyclin D1, and c-Myc; this indicates that ERα ufmylation is required for its transactivation function. In addition, the 2KR mutation prevented anchorage-independent colony formation by MCF7 cells. Most notably, the expression of UFM1 and its conjugating machinery (i.e., UBA5, UFC1, UFL1, and UFBP1) were dramatically upregulated in ERα-positive breast cancer cell lines and tissues. Collectively, these findings implicate a critical role attributed to ERα ufmylation in breast cancer development by ameliorating its stability and transactivity.

• 대한간호학회지
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• 제34권7호
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• pp.1277-1287
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• 2004

Purpose: This study was to evaluate the effects of a short term comprehensive life style modification program on glycemic metabolism, lipid metabolism and body composition in type 2 diabetes mellitus patients. Method: A nonequivalent control group with a pre post test was designed. Data collection was done from October 2003 to June, 2004 at a hospital. Glycemic metabolism was measured by a.c., p.c. and $HbA_{1}c$, and lipid metabolism was measured by cholesterol, HDL cholesterol, LDL cholesterol and triglycerides. Body Composition was measured by body weight, body mass index, waist, measurement waist per hip ratio, body fat, muscle weight and abdominal fat tissue(intra abdominal distance). The Experimental group, which was composed of 29 participants, was educated based on a life style modification protocol at a weekly meeting for 12 weeks and carried out exercise, diet along individual parameters and self monitoring, while 24 participants in the control group received only diet education. Result: 1. The experimental group showed a significant lower a.c.(t=2.11, p=.04) and $HbA_{1}c$(t=2.65, p=.01) compared to those of the control group. 2. The experimental group showed a significant lower LDL than the results of the control group(t=2.42, p=.02). 3. The experimental group showed a significant lower weight(t=3.09, p=.00), BMI(t=3.01, p=.00), body fat(t=2.94, p=.01) and abdominal fat tissue(t=3.05, p=.01) than those of the control group. Conclusion: The results provided evidence for the effectiveness of a short term comprehensive life style modification program composed of exercise, diet, support, self efficacy elevation and self monitoring in type 2 diabetes mellitus.

• 한국주거학회:학술대회논문집
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• 한국주거학회 2011년도 춘계학술발표대회 논문집
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• pp.172-175
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• 2011

Financial supports for housing for the disabled is limited since a certain amount of budget needs to be distributed for a number of disabled families. Therefore, it is important to fund renovation for the disabled effectively and properly. Financial and administrative supports for the renovation which intended to improve housing conditions in terms of accessibility (barrier free) and comfort needs to be implemented with larger outcome and smaller budget. The purpose of this study was to investigate various factor such as renovation cost and renovation elements, influence satisfaction of residents with disabilities. Participants of this study are limited to the recipients of financial supports for housing renovation and refurbishment from City of Seoul and Ministry of Heal and Welfare. A total of 155 cases were analyzed using post occupancy survey and analysis of project cost data. Overall, more cost were used for modification for housing condition improvement than the cost for removal of architectural barriers. Renovation cost differences were not apparent among the type and extent of an individual's disabilities. Instead existing housing type and physical condition for the housing affects cost for renovation more significantly. Overall the renovation cost is positively related to resident's satisfaction using regression analysis.

• 한국항공우주학회지
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• 제41권11호
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• pp.865-873
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• 2013

본 논문에서는 유한요소해석에 의한 막재료의 주름 해석 기법에 관하여 연구하였다. 삼각형 세일 형상에 대해 멤브레인 요소와 쉘 요소를 사용하여 주름해석을 수행하였다. 멤브레인 요소를 이용한 기법에서는 주름을 벌칙매개변수에 의한 물성치를 수정하는 알고리즘을 상용프로그램 내 사용자 서브루틴을 통하여 구현하였다. 쉘 요소에 의한 기하학적 비선형 후좌굴 기법에서는 면외방향의 좌굴을 발생시키기 위하여 모델의 메쉬에 작은 크기의 기하학적 결함을 심는 방법을 사용하였다. 쉘 방법에서는 내연 및 외연해석 기법을 고려하였다. 요소수의 증가에 따른 수렴성과 결과의 정확도의 관점에서 멤브레인 요소법과 쉘 요소법의 효율성을 비교하였다.