• Title/Summary/Keyword: Positive sequence

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Isolation of the Threonine Dehydratase Gene from a Tylosin-Producing Strain of Streptomyces fradiae

  • Lee, Sang Hee;Kye Joon Lee
    • Journal of Microbiology and Biotechnology
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    • v.5 no.5
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    • pp.305-308
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    • 1995
  • From the plasmid library made from Sstl and San-digested genomic DNA of Streptomyces fradiae NRRL 2702, four positive clones were selected using an oligodeoxynucleotide probe from the N-terminal amino acid sequence of purified threonine dehydratase. The cloned gene for threonine dehydratase was a 2.0 kilo-base pair DNA fragment. The deduced amino acid sequence of PCR product (PCR245) was matched to that of the N-terminal part of threonine dehydratase from S. fradiae and this showed a high similarity to the threonine dehydratases of other organisms. This indicated that amino acid sequences of threonine dehydratases were highly conserved and the polypeptide product of the PCR245 was likely to be involved in the deamination of threonine.

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345/154 kV Transmission Line model choice and calculation using TMLC (TMLC용 345, 154kV 송전선로 모델 작성 및 계산)

  • Choi, H.K.;Moon, Y.H.;Yoon, J.Y.;Choo, J.B.;Yun, Y.B.;Kim, Y.H.
    • Proceedings of the KIEE Conference
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    • 2001.07a
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    • pp.336-339
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    • 2001
  • Transmission line data are very important for studying loadflow. Short circuit data(positive sequence, zero sequence) of 345kV and 154kV line were calulated and compared with KEPCO's line characteristics data. This Paper presents method of verification and complement of line data in PSS/E loadflow data using TMLC (Transmission Line Characteritics) program.

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A Control Strategy to Obtain Sinusoidal Input Currents of Matrix Converter under Unbalanced Input Voltages

  • Nguyen, Thanh-Luan;Lee, Hong-Hee
    • Proceedings of the KIPE Conference
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    • 2018.07a
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    • pp.114-116
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    • 2018
  • This paper presents a control strategy to achieve the balanced sinusoidal output currents, as well as sinusoidal input currents for the matrix converter (MC) under unbalanced input voltages. By regulating the modulation index of the converter according to the instantaneous input voltages, the output currents are kept balanced and sinusoidal. In order to obtain sinusoidal input currents, the input power factor angle should be dynamically calculated based on the positive and negative sequence components of the input voltages. This paper proposes a simple method to construct the expected input power factor angle without the complicated sequence component extraction of input voltages. Simulation results are given to validate the effectiveness of the proposed control strategy.

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Fault Detection Relaying for Transmission line Protection using ANFIS (적응형 퍼지 시스템에 의한 송전선로보호의 고장검출 계전기법)

  • 전병준
    • Journal of the Korean Institute of Intelligent Systems
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    • v.9 no.5
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    • pp.538-544
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    • 1999
  • In this paper, we propose a new fault detection algorithm for transmission line protection using ANFIS(Adaptive Network Fuzzy Inference System). The developed system consists of two subsystems: fault type classification, and fault location estimation. We use rms value, zero sequence component and positive sequence of current, and then using learning method of neural network, premise and consequent parameters are tuned properly. To prove the performance of the proposcd system, generated data by EMTP(Electr0- Magnetic Transient Program) sin~ulationi s used. It is shown that the proposed relaying classifies fault types accurately and advances fault location estimation.

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Analysis of Dynamic Characteristics of Three Phase Induction Motor Applied by Various Unbalanced Voltage (다양한 불평형 전압이 인가된 3상 유도전동기의 동적특성 분석)

  • Kim, Tae-Han;Jwa, Chong-Keun
    • Proceedings of the KIEE Conference
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    • 2003.07b
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    • pp.810-812
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    • 2003
  • The dynamic characteristics of a three phase induction motor which is applied by various unbalanced voltages are simulated and analyzed in this paper. The voltage equations and torque equation in the dq stationary reference frame for the three phase star connected induction motor are used in this analysis. MATLAB and SIMULINK are employed as a simulation tool. The dynamic characteristics of speed and torque are simulated for various unbalanced voltages, that is, (1) cases with the same unbalance voltage factor but different unbalanced voltages, (2) cases with only one unbalanced voltages but different degree of unbalance, and (3) cases with the same positive sequence voltage but different negative sequence voltages. The simulated results are compared and analyzed with each other, and also with the results of balanced voltage.

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An Application of Proportional-Resonant Controller in MMC-HVDC System under Unbalanced Voltage Conditions

  • Quach, Ngoc-Thinh;Ko, Ji-Han;Kim, Dong-Wan;Kim, Eel-Hwan
    • Journal of Electrical Engineering and Technology
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    • v.9 no.5
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    • pp.1746-1752
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    • 2014
  • This paper presents an application of proportional-resonant (PR) current controllers in modular multilevel converter-high voltage direct current (MMC-HVDC) system under unbalanced voltage conditions. The ac currents are transformed and controlled in the stationary reference frame (${\alpha}{\beta}$-frame). Thus, the complex analysis of the positive and negative sequence components in the synchronous rotating reference frame (dq-frame) is not necessary. With this control method, the ac currents are kept balanced and the dc-link voltage is constant under the unbalanced voltage fault conditions. The simulation results based on a detailed PSCAD/EMTDC model confirm the effectiveness of the proposed control method.

Fast Extraction of Symmetrical Components from Distorted Three-Phase Signals Based on Asynchronous-Rotational Reference Frame

  • Hao, Tianqu;Gao, Feng;Xu, Tao
    • Journal of Power Electronics
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    • v.19 no.4
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    • pp.1045-1053
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    • 2019
  • A symmetrical component decomposition scheme utilizing the characteristics of the asynchronous rotational reference frame transformation is proposed in this paper for the extraction of the positive and negative sequence components of distorted three-phase grid voltages. The undesired frequency component can be removed using a specially designed series coordinate transformation and half-cycle delays, where the delay can be controlled by adjusting the frequency of the rotating reference frame. The extracted symmetrical component can then be compensated based on the applied coordinated transformation. The dynamic response of the proposed algorithm is improved when compared to that of conventional methods. The effectiveness of the proposed algorithm is verified by simulation and experimental results.

Whole genome sequence analysis of Ligilactobacillus agilis C7 isolated from pig feces revealed three bacteriocin gene clusters

  • Jeong Min, Yoo;Remilyn M., Mendoza;In-Chan, Hwang;Dae-Kyung, Kang
    • Journal of Animal Science and Technology
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    • v.64 no.5
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    • pp.1008-1011
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    • 2022
  • We here report the whole genome sequence of Ligilactobacillus agilis C7 with anti-listerial activity, which was isolated from pig feces. The genome size of L. agilis C7 (~ 3.0 Mb) is relatively larger compared with other L. agilis strains. L. agilis C7 carries three bacteriocin gene clusters encoding garvicin Q, salivaricin A, and Blp family class II bacteriocin. Garvicin Q and salivaricin A are reported to be active against Listeria monocytogenes and Micrococcus luteus, respectively, as well as against other Gram-positive bacteria. Meanwhile, the bacteriocin encoded in the blp cassette was shown to be active against pneumococci, mediating intraspecies competition. This report highlights the potential of L. agilis C7 for the production of bacteriocins inhibiting pathogenic bacteria.

Effects of Lay Rationalism, Attitude Dimension and Involvement Type on Intent to Purchase Hedonic Product

  • CHOI, Nak-Hwan;CAI, Yunwei;LI, Zhonghua
    • Journal of Distribution Science
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    • v.17 no.8
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    • pp.45-56
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    • 2019
  • Purpose - This study aimed at investigating the mediation roles of attitude dimensions in the effects of involvement type on hedonic product purchase intention and moderation role of lay rationalism in the effects of involvement type on attitude dimensions. Research design, data, and Methodology - "Wenjuanxing" was used online to make questionnaire, which was loaded on Wechat and QQ. 125 data were collected online in China. The Process macro model 58 including moderation of the two paths in the causal sequence was used to verify hypotheses. Results and Conclusions - First, cognitive (affective) involvement had positive effect on the utilitarian (hedonic) dimension of consumer attitude and the purchase intention. Second, hedonic dimension of attitude had positive effects on purchase intention, but utilitarian dimension of attitude had not significant positive effects on purchase intention. Third, Lay rationalism did decrease (did not increase) the positive effects of affective (cognitive) involvement on hedonic (utilitarian) dimension of attitude. Therefore Marketing managers should understand the differences between the cognitive involvement and affective involvement, and develop the ways by which they attract consumers to choose their hedonic product. And they should give affective (cognitive) information to the customers with low (high) rationalism consumers when they do marketing for their hedonic product.

Prevalence of negative frequency-dependent selection, revealed by incomplete selective sweeps in African populations of Drosophila melanogaster

  • Kim, Yuseob
    • BMB Reports
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    • v.51 no.1
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    • pp.1-2
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    • 2018
  • Positive selection on a new beneficial mutation generates a characteristic pattern of DNA sequence polymorphism when it reaches an intermediate allele frequency. On genome sequences of African Drosophila melanogaster, we detected such signatures of selection at 37 candidate loci and identified "sweeping haplotypes (SHs)" that are increasing or have increased rapidly in frequency due to hitchhiking. Based on geographic distribution of SH frequencies, we could infer whether selective sweeps occurred starting from de novo beneficial mutants under simple constant selective pressure. Single SHs were identified at more than half of loci. However, at many other loci, we observed multiple independent SHs, implying soft selective sweeps due to a high beneficial mutation rate or parallel evolution across space. Interestingly, SH frequencies were intermediate across multiple populations at about a quarter of the loci despite relatively low migration rates inferred between African populations. This invokes a certain form of frequency-dependent selection such as heterozygote advantage. At one locus, we observed a complex pattern of multiple independent that was compatible with recurrent frequency-dependent positive selection on new variants. In conclusion, genomic patterns of positive selection are very diverse, with equal contributions of hard and soft sweeps and a surprisingly large proportion of frequency-dependent selection in D. melanogaster populations.