• Proceedings of the PSK Conference
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• 2000.10a
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• pp.247.1-247.1
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• 2000

• KSBB Journal
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• v.18 no.3
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• pp.165-169
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• 2003

The antioxidative activity of Portulaca oleracea was tested using in vitro experimental models. Antioxidative activities were determined by measuring DPPH radical scavenging activity and lipid peroxide using 2-thiobarbituric and (TBA). The crude extract was sequentially partitioned with n-hexane, 15% aq. MeOH, EtOAc, n-BuOH, $H_2O$. A remarkable antioxidative effect was observed in the EtOAc and n-BuOH fractions. The DPPH radical scavenging effect ($IC_{50}$=17.90 $\mu\textrm{g}$/ml) of the n-BuOH soluble fraction was comparable with that of the natural antioxidant, $\alpha$-tocopherol ($IC_{50}$=6.99 $\mu\textrm{g}$/ml) and the inhibition effect of lipid peroxidation in mouse liver homogenate was similar to that of the natural antioxidant, L-ascorbic acid at a concentration of 1.0 mg/ml to 5 mg/ml.

• Journal of Oriental Neuropsychiatry
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• v.24 no.3
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• pp.271-280
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• 2013

Objectives : The development of natural drugs with antidepressant effects is important and needed. This study was performed to investigate the antidepressant-like effects of the distilled water extract of Portulaca oleracea L. (POL) in a mouse model and to investigate the role of ${\alpha}$-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) receptors in producing these antidepressant-like effects. Methods : The forced swim test (FST) and tail suspension test (TST) were used to investigate the behavioral anti-depressive-like effects of POL in mice. Additional behavioral experiments with 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline-2,3-dione, an AMPA receptor antagonist, were undertaken to determine the involvement of the antidepressant-like properties of POL in AMPA receptor throughput. Results : Oral administration of the POL extract (100 mg/kg) 1 h prior to testing significantly reduced the immobility times in the FST and TST. The antidepressant-like effects of the POL extract were not increased in a dose-dependent manner. Pre-treatment with NBQX significantly attenuated the reduction in immobility time induced by the POL extract in the FST. Conclusions : The distilled water extract of POL has antidepressant-like effects, which may be related to AMPA receptor. Pre-treatment with NBQX significantly attenuates the reduction in immobility time induced by the POL extract in the FST.

• KSBB Journal
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• v.24 no.4
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• pp.397-402
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• 2009

The Portulaca oleracea (P. oleracea) is a popular herbal medicine in East Asia that was known to possess detoxification, antifebrile and antifungal effects. In the present study, we examined the biological activities of ethanol extracts of P. oleracea under various conditions with NIH3T3, B16F10, and MCF-7 cell line model systems. Extracts of P. oleracea (0.5 mg/ml) showed inhibition of expression of tyrosinase, but does not suppress either of TYRP-1 or DCT expression on B16F10 cells. Extracts of P. oleracea (2 mg/ml) showed anti-inflammatory effects on TNF-$\alpha$-stimulated NIH3T3/$NF{\kappa}B$-Luc cells and increase of the synthesis of collagen on NIH3T3 (wild type) cells. These results suggest that extracts of P. oleracea could be used as a functional biomaterial in developing a skin whitening agent and having the anti-inflammatory, anti-wrinkle, and anti-aging activities.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.7
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• pp.277-284
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• 2020

This study was undertaken to determine the effects of dietary supplementation with herbal extracts of Portulaca oleracea L. (POL), on the reproductive performance, blood profiles, immune response, and fecal microbial population in multiparous sows. On the basis of initial body weight, a total of 20 multiparous sows (Landrace×Yorkshire; 229.2±1.15 kg) were randomly allotted to 2 treatment groups, each including 10 replicates. The dietary treatments were administered during the lactation period, and included a corn-soybean meal-based diet (control group) and diet supplemented with 0.05% POL. Throughout the experimental period, no significant differences were observed between the two diet groups for body weight, backfat thickness, and reproductive performance. Blood profile analysis revealed significantly decreased lymphocyte concentrations and IL-1β levels (p<0.05), and increased serum IgG levels (p=0.051), of sows consuming the POL supplemented diet. Furthermore, coliform counts tended to decrease in sows consuming diet supplemented with POL (p=0.063). Taken together, our results indicate that POL supplemented diets exert beneficial effects on blood profiles, immune response, and fecal microflora of multiparous sows, without any negative effect on the reproductive performance.

• The Korean Journal of Ecology
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• v.4 no.3_4
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• pp.114-123
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• 1981

Diurnal acid fluctuations, stomatal resistance, and morphology and anatomy were investigated in leaves and stems P. oleracea L. growing under the natural environmental condition. A CAM-like pattern of acid fluctuation was exhibited not in leaves of the young purslane but in its stems. Defoliated stems showed a decreased in diurnal changes of total acidity as compared with normal stems. Excised stems stored in continuous darkness exhibited diurnal acid rhythms, and they showed light deacidifications for three days. Kranz-type arrangement was observed in leaves, but not in stems. Micrography of cross sections of stems showed cells with relatively large vaculoles and a few chloroplasts. The number of stomata was 3,275cm-2 in leaves, while the stomata could not be observed in stems. Stomatal resistance was high at night and low in daytime in leaves of the young purslane, and the range of its value was 5~40 sec.$\textrm{cm}^{-1}$. But stomatal resistance in leaves of the water-stressed plant was comparratively high in day time, and its value was 30 sec.$\textrm{cm}^{-1}$. The result of these studies showed the possibility that the stem of P.oleracea L. possesses CAM under certain stressed conditions.

• Current Research on Agriculture and Life Sciences
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• v.9
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• pp.115-125
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• 1991

The medicinal plants of 33 species with symptoms of mosaic, necrosis, line pattern, malformation (fine leaf), stripe, and ring spot infected by cucumber mosaic virus spontaneously, were collected in Korea and Japan. Among them, 2 mosaic diseases of portulaca oleracea L. and Celocia argentea L., 2 necrotic mosaic diseases of Aristolochia debilis Sieb. and Tetragonia exponsa Murr., 6 mottle diseases of Cassia torosa Cav., Bupleurum falcantum L., Angelica acutioloba Kitagawa, A. keiskei Koidz., Peucedanum japonicum Thunb., and Foeniculum vulgare Mill., and ringspot disease of Basella rubra L., were named newly in these studies.

• Nutrition Research and Practice
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• v.12 no.3
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• pp.183-190
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• 2018

BACKGROUND/OBJECTIVE: This study was designed to investigate how a Portulaca oleracea L. extract (POE) stimulates insulin secretion in INS-1 pancreatic ${\beta}-cells$. MATERIALS/METHOD: INS-1 pancreatic ${\beta}-cells$ were incubated in the presence of various glucose concentrations: 1.1 or 5.6, 16.7 mM glucose. The cells were treated with insulin secretagogues or insulin secretion inhibitor for insulin secretion assay using an insulin ELISA kit. In order to quantify intracellular influx of $Ca^{2+}$ caused by POE treatment, the effect of POE on intracellular $Ca^{2+}$ in INS-1 pancreatic ${\beta}-cells$ was examined using Fluo-2 AM dye. RESULTS: POE at 10 to $200{\mu}g/mL$ significantly increased insulin secretion dose-dependently as compared to the control. Experiments at three glucose concentrations (1.1, 5.6, and 16.7 mM) confirmed that POE significantly stimulated insulin secretion on its own as well as in a glucose-dependent manner. POE also exerted synergistic effects on insulin secretion with secretagogues, such as L-alanine, 3-isobutyl-1-methylxanthine, and especially tolbutamide, and at a depolarizing concentration of KCl. The insulin secretion caused by POE was significantly attenuated by treatment with diazoxide, an opener of the $K{^+}_{ATP}$ channel (blocking insulin secretion) and by verapamil (a $Ca^{2+}$ channel blocker). The insulinotropic effect of POE was not observed under $Ca^{2+}$-free conditions in INS-1 pancreatic ${\beta}-cells$. When the cells were preincubated with a $Ca^{2+}$ fluorescent dye, Fluo-2 (acetoxymethyl ester), the cells treated with POE showed changes in fluorescence in red, green, and blue tones, indicating a significant increase in intracellular $Ca^{2+}$, which closely correlated with increases in the levels of insulin secretion. CONCLUSIONS: These findings indicate that POE stimulates insulin secretion via a $K{^+}_{ATP}$ channel-dependent pathway in INS-1 pancreatic ${\beta}-cells$.

• Korean Journal of Agricultural Science
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• v.11 no.2
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• pp.190-193
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• 1984

Extracts of common purslane(Portulaca oleracea L.) showed to possess some antifungal substances which inhibited the mycelial growth of the phytopathogenic fungi tested;Valsa mali, Alternaria kikuchiana and Pyricularia oryzae. These antifungal substances were found to be soluble in methanol and were regarded as kinds of lipid. In order to isolate the antifungal substances, the extracts of common purslane were concentrated by evaporation under reduced pressure and extracted with methanol The methanol solution was subjected to silica gel-florisil column and divided into lipid and non-lipid fractions. Lipid fractions only showed antifungal activity against the fungi tested. The effective substances contained in the extracts of common purslane inhibited not only the mycelial growth but also the spore germination of the fungi.

• Korean Journal of Weed Science
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• v.9 no.1
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• pp.76-79
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• 1989

As a result of screening search for biologically active substances to weed seeds among higher plants, MeOH extract from Rhathiolepis ovata Briat was found to inhibit germination of test weeds considerably. In the course of purifying the active substances, pinoresinol which showed very similar behavior with the active fraction on various chromatographies, was isolated from the same source, spectrally identified and bioassayed. Pinoresinol exhibited germination inhibitory activity against the common purslane (Portulaca oleracea L.) only ; the inhibitory effect was about 55% at concentration of 5 mg/ml.