• 기술사
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• 제32권1호
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• pp.90-98
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• 1999

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.97-98
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• 2003

• 환경위생공학
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• 제10권1호
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• pp.126-131
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• 1995

Chitinase, a potential pathogensis- related portein, was induced in the leaves of 30 day - old bean( phaseolus vulgaris ) plant with the treatment of 10 $\mu $ /mℓ ethylen for 30hrs. Chitinase was purified from the mature tissue of bean leaves( phaseolus vulgaris ) by ammonium sulfate precipitation followed by affinity chromatography on a regenerated chitin and sephadex G-75 chromatography. The purified chitinase gave a single band SDS- PAGE to be 32,000 Dalton. In order to elucidate the three- dimensional structure of chitinase and to shed light on the functional mechanism of this class of enzymes, the enzyme was tried to crystallize( Sitting Drop Method). Crystals grew at room temperature to their final size within two weeks(0.2mm $\times $0.2mm $\times $ 0.1 mm ), This enzyme are still continuing to crystallize for the study of X- ray.

• Toxicological Research
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• 제14권3호
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• pp.365-370
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• 1998

To evaluate the renal toxicity of the antitumor agent, p-{N,N,-Bis(2-chloroethyl)amino}-4-phenyl acetyl-amino-2,6-piperidinedione(CK-15), rats were treated with CK-15 (acute: 50mg/kg. i.p., single and subacute: 5mg/kg, i.p., daily for 7 days). The changes in the body weight, water consumption, kidney weights and urine volume after and during the treatment were observed. The concentrations of urinary creatinine and portein, the activities of N-acetyl-${\beta}$-D-glucosaminidase (NAG), alanine aminopeptidase (AAP), ${\gamma}$-glutamyl transpeptidase (${\gamma}$-GT) and lactate dehydrogenase (LDH) in 24hr urine were also determined. The body weight, water consumption, and urine volume were decreased after the acute and subacute administration. However the weights of kidney were not changed after the treatments. The excretion of creatinine was significantly decreased 1 day after acute administration but, returned to the control value. In subactute administration, the excretion of creatinine was gradually decreased. However, the protein excretion did not changed in both treatment. Those indicate that CK-15 might decrease the metabolic rate of muscle. THe urinary activities of NAG, AAP, ${\gamma}$-GT, and LDH were significantly affected bythe drug treatment. The urinary activities of NAG, AAP and ${\gamma}$-GT were significantly increased 1 day after the acute administration and then returned to the control value. However, the urinary activities of LDH were not changed in acute treatment. In subacute treatment, although the urinary activities of NAG were not changed, those of AAP and ${\gamma}$-GT were significantly increased 2.3 times at 3 days during the subacute administration. Also the urinary activities of LDH were significantly increased at 7 day after the administration. These results indicate that the high and subacute administration might induce a damage in the kidney cells. Furthermore the present results suggest that the toxic effects of CK-15 might be due to the accumulation of the metabolites.

• Journal of Nutrition and Health
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• 제19권1호
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• pp.32-40
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• 1986

The present study conducted to examine the effects of proteins from different sources on Ca excretion in 6 healthy young adult Korean women. The subjects were given meat protein diet for 5 days and soy protein diet for subsequent 5 days. the two diets were similar in protein and Ca contents. Urinary and fecal samples were collected for the last 2 days of each diet period and were analyzed for Ca and P. The results were as following ; 1) Mean daily urinary calcium excretion was 126.5$\pm$22.70mg for meat protein diet and 83.7$\pm$17.19mg for soy protein diet and the difference was significant (P<0.025). 2) Fecal Ca excretion did not show significant difference between two experimental preiod ; 466.9$\pm$73.68 mg of meat portein diet 284.4$\pm$54.96mg for soy protein diet. 3) Three out of six subjects showed negative balance on meat protein diet, but only one showed negative balance on soy protein diet. The averageof the balances on meat protein diet was -65.4 $\pm$68.19 and that of soy protein diet was 155.3$\pm$52.28 ; the difference was significant(P<0.025). 4) Urinary P excretion tended to be higher on meat protein diet but was not significant compared to soy protein diet . Fecal P excretion was significantly higher (P<0.001) on soy protein diet. Overall P balances for meat protein diet and soy protein diet were 219.94 mg and 229.46mg respectively (P<0.05). The above results show that urinary Ca excretion was significantly higher on meat protein diet but fecal excretion did not show significant difference between meat protein diet and soy protein diet. The overall Ca balance was significantly higher on Soy protein diet compared to meat protein diet.

• Preventive Nutrition and Food Science
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• 제4권1호
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• pp.47-51
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• 1999

In order to evaluate the dietary regulation of cysteine sulfinic acid decarboxylase (EC 4.1.1.29) in cats, acitivity and protein content of CSAD were assessed in the liver and kidney of cats fed different levels of dietary protein, with and without taurine. Four groups of cats were fed one of the follow diets for 5 weeks ; 20% protein and taurine- free diet(LP0T) ; 20% protein and 0.15% taurine diet(LPNT) ; 60% protein and taurine-free diet(HP0T); and 60% protein and 0.15% taurine diet (HPNT). CSAD activity was determined in the liver and kidney of cats by measuring 14C2 released form [1-14C]-L cysteine sulfinic acid. CSAD protein was quantified using an immunochemical method. CSAD activity was extremely low in cat tissues, among which kidney showed the highest activity which was 0.118$\pm$0.050, and 0.377$\pm$0.056 nmol.min-1.mg soluble portein-1 iin animals fed LP0T and HP0T, respectively. Even though renal CSAD protein content was 18~55% of the hepatic CSAD protein content, renal CSAD acitivity was 1.3~6.5 times of the hepatic CSAD activity . Renal CSAD acitivities of cats fed 60% protein were about 1.6~3.2 times those of animals fed 2.% protein , and hepatic CSAD activity was not significantly affected by the dietary level of protein. Taurine depletion significantly elevated both hepatic and renal CSAD activities above the values for cats having normal taurine status most probably as an adaptive response.

• 생약학회지
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• 제49권2호
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• pp.182-187
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• 2018

Many plant derived phytochemicals have been considered as the main therapeutic strategy against Alzheimer's disease (AD). AD is a progressive neurodegenerative disorder, and the most predominant cause of dementia in the elderly. Cholinergic deficit, senile plaque/${\beta}$-amyloid ($A{\beta}$) peptide deposition and oxidative stress have been identified as three main pathogenic pathways which contribute to the progression of AD. We screened many different plant species for their effective use in both modern and traditional system of medicines. In this study, we tested that MeOH extract of the stem bark of Hopea chinensis (Merr.) Hand.-Mazz. (HCM) affects on the processing of Amyloid precursor portein (APP) from the APPswe over-expressing Neuro2a cell line. We showed that HCM reduced the secretion level of $A{\beta}42$ and $A{\beta}40$ in a dose dependent manner. We found that HCM increased over 1.5 folds of the secretion level of $sAPP{\alpha}$, a metabolite of ${\alpha}$-secretase. Furthermore, we found that HCM inhibited acetylcholinesterase activity in vitro. We suggest that the stem bark of Hopea chinensis may be a useful source to develop a therapeutics for AD.

• 한국초지조사료학회지
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• 제12권3호
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• pp.185-192
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• 1992

This experiment was conducted to compare the productivity of five silage corn varieties. Corn varieties were Pioneer 3144(P 3144), 3160(P 3160), 3282(P 3282), 3352(P 3352) and Suweon 19(S 19) and were seeded on May 2 and harvested on August 15. Ratios of stalk, leaf and ear, chemical composition and yields of nutrients were investigated in respective corn part. Experimental design was accepted by Latin square arrangement with five replicates and each plot had twenty two plants. The results obtained were as follows: 1. Crude portein, crude ash and nitrogen free extract(NFE) contents of whole plant ranged from 6.32 to 7.18%, 5.16 to 7.43% and 52.62 to 57.90%, respectively, and there were no significant(P>.Ol) differences between varieties. Crude protein and NFE content were the highest in ear between corn parts. 2. Crude fiber conent of whole plant ranged from 18.98 % to 24.01 %, and was the highest in suweon 19 and the lowest in P 3352(P<.01). Crude fiber content was the highest in stem and the lowest in ear, However, crude fat content(2.03-3.66 %) vice versa. 3. Yields of organic matter, crude protein, crude fat, NFE and TDN per 10a were the greatest(P< .01) in P 3282, however crude fiber yield was the highest(P<.Ol) in P 3352. 4. Ratios of different part to total dry matter yield ranged from 12.7 %(P 3352) to 17.8 %(P 3160) for leaf, 44.5(P 3352) to 66.9 %(P 3160) for stem and husks, and 16.9 %(P 3282) to 42.8 %(P 3352) for ear, and there were significant differences between corn varieties(P<.Ol). Consequently, P 3352 has the greatest nutrient yields, particularly by ear, and P 3282 has a great nutrient yield by stem. This result suggested that could be appear a better character of silage corn by hybridization of this two corn varieties.

• 대한영상의학회지
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• 제84권2호
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• pp.498-503
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• 2023

고환 핵단백질 정중선 암은 매우 드물고 분화가 나쁜, 악성 편평세포암의 한 하위 종양으로서 공격적으로 분화하여 예후가 좋지 않다. 특히 폐에서 발생하는 고환 핵단백질 정중선 암은 현재까지 국내에 보고된 증례가 많지 않고 다양한 조직학적 형태를 보일 수 있어 다른 암과 혼동될 수 있다. 영상의학적 소견의 보고는 전 세계적으로 매우 드문 편이다. 저자들은 25세 여성에서 발생한 폐 고환 핵단백질 정중선 암의 영상의학적 소견을 병리학적인 소견과 함께 보고하고자 한다.

• Reproductive and Developmental Biology
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• 제35권3호
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• pp.273-280
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• 2011

It was conducted the experiment, divided into three groups as normal, poor and polycystic ovary syndrome, to detect the change of protein patterns in follicular fluid on ovarian response following controlled ovarian hyperstimulation for human IVF outcome. In the normal group, it was confirmed reproducible 57 spots in the detected total 81 spots. Then 1 spot was not found in the other groups. In the poor responder group, it was found reproducible 53 spots in the detected total 98 spots. 6 spots were down-regulation and 7 spots were up-regulation comparable with normal group. There were not 5 spots in poor responder group comparable with other groups. In the polycystic ovary syndrome group, it was expressed reproducible 53 spots in the detected total 80 spots and 3 spots were just expressed in this group. However, 4 spots were not found in polycystic ovary syndrome. 9 spots were up-regulation comparable with normal group. Significant up and down-regulation spots among the each groups were identified. The results were a cytosolic carboxypeptidase, a signal-induced proliferation-associated protein 1, a ceruloplasmin, a keratin(type II cytoskeletal 1), a polypeptide N-acetylgalactosantinyltransferase 2, a serine/threonine-protein phosphatase 4 regulatory subunit 4. It was identified that 8 spots, 6 kinds of protein are corresponded with NCBInr database research, but 10 spots were failed in the identification. In conclusion, it has been confirmed change and expression of protein on the ovarian response following COH of human.